[Pathomorphology of experimental ornithosis pneumonia]. / Patomorfologiia éksperimental'noi ornitoznoi pnevmonii.

Model experiments in white mice reproduced chronic ornithosis pneumonia morphological features of which included early outgrowth of connective tissue cells of the type of carnification, considerable accumulation of tropoglycogen, and disturbed process of collagen fibers formation at a minimal number of leukocytes. Such foci occur in the lungs very early, before the onset of the clinical signs of the disease which appears to be a typical feature of ornithosis pneumonia and may explain causes and mechanisms of frequent occurrence of chronic forms of this disease. In cases of significant multiplication of the ornithosis agent there is mass migration of leukocytes into the focus of lesion followed by the development of acute inflammation. Being an obligatory intracellular parasite, the ornithosis agent affects both large and small alveolar cells. Multiplying by binary division, it stays free in the cytoplasm without forming a common vacuole. Ultrastructurally typical various forms of the ornithosis agent reflecting the stages of its complicated life cycle have been detected.

[Pathological anatomy and pathogenesis of small intestine lesions due to opportunistic enterobacteria (Proteus and Klebsiella)]. / Patologicheskaia anatomiia i patogenez porazhenii tonkoi kishki, vyzvannykh uslovno-patogennymi énterobakteriiami (proteem i klebsielloi).

Infection with opportunistic agents was reproduced in rabbits by introduction of Proteus and Klebsiella bacteria into a section of the narrowed (but not ligated) small intestine. The presence of infection was documented by clinical signs, isolation of the causative agents, and immunological changes. Light and electron microscopy revealed an inflammatory process of the type of catarrhal enteritis with predominant involvement of the epithelial sheet ("epithelitis") and reactive changes in the base of the mucous membrane and submucous layer. Morphological differences of the infections caused by Proteus and Klebsiella were demonstrated. The level and intensity of pathological changes appear to be associated with the degree of conditionality of the pathogenic properties of enterobacteria.

[Study of the process of interaction of the causative agent with the cells of the body and with a macrophage culture in experimental typhoid infection and carrier state]. / Issledovanie protsessa vzaimodeistviia vobuditelia s kletkami organizma i kul'turoi makrofagov pri éksperimental'noi briushnotifoznoi infektsii i nositel'stve

Experiments were conducted on 117 rabbits and cells of the macrophage cultures in vitro by the methods of clinico-laboratory, quantitative microbiological, immunological, electron microscopic and microcinematographic examination; a study was made of the interaction of the typhoid causative agent with the cells of the organism and the macrophage cultures and also of some aspects of the immune response during acute typhoid infection and carrier state. Infection was modelled by the enteral, subconjunctival and intrabonemarrow infection with 24-hour culture of the typhoid bacillus (strain Ty2 4446). Experiments demonstrated that structural reconstruction of both the causative agent and of the cells of the organism, of the culture macrophages and their organoids occurred in the course of the first hour after the infection. Homogenates of the lymphoid and myeloid tissues and also of the macrophages and polymorphonuclears possessed bactericidal activity against S. typhi. The degree of this activity largely depended on the pH of the medium. It was also shown that under conditions of the macrophage culture sodium aside inhibited the bactericidal activity of macrophages obtained from the intact and immune animals.