ABSTRACT
PURPOSE: The study aimed to analyze whether adding Cissus quadrangularis (CQ) extract and the extracellular matrix of ovine tendon (TENDON) increases the regenerative potential of mesenchymal stem cells produced in hyaluronic acid (HA) scaffolds for tenogenesis. MATERIALS AND METHODS: Fifty grams of powdered CQ was mixed with 250 mL of ethanol to prepare the extract. Two grams of hyaluronic acid powder was added to 100 mL of distilled water to make the HA solution. The ovine tendon was decellularized using a mixture of 10% phosphate-buffered saline (PBS), sodium dodecyl sulfate (SDS), and Triton-X. The hydrogel samples were prepared by mixing the extracellular matrix of tendon, HA, and CQ, after which they were divided into study groups such as HA, HA + CQ, HA + TENDON, and HA + CQ + TENDON. Scanning electron microscopy (SEM) analysis, swelling analysis, differentiation analysis, compression test, compatibility assay, and tenogenesis assay were later conducted. RESULTS: The morphology of the samples was analyzed using SEM. Low levels of swelling of the hydrogels were observed. Cells were found to be viable and showed good differentiation and tenogenesis. Optimal compression levels were observed, and the properties of the prepared hydrogels were satisfactory. CONCLUSION: The results suggest that the addition of CQ considerably increases the tenogenic potential of the extracellular matrix/HA scaffold. Hence, it can be used as a regenerative material for periodontal tissue regeneration.
ABSTRACT
Background Ocimum sanctum (OS) is a medicinal plant with antioxidant, anti-cancer, anti-diabetic, antimicrobial, and anti-inflammatory activities. Extracellular matrix (ECM) maintains the structural stability of tissues. Hyaluronic acid (HA), which is used in hydrogel fabrication and osteochondral regeneration, increases cell viability and the expression of marker genes. Periodontal ligament stem cells (PDLSC), which are a type of mesenchymal stem cells (MSC), have self-renewing capacity and they prevent teratoma formation and promote tendon (TEN) regeneration. The aim of this study is to incorporate the phytochemical effects of Ocimum sanctum into hyaluronic acid hydrogel scaffolds made with the MSCs in tendon ECM for increased tissue regeneration. Materials & methods Ocimum sanctum extract and methacrylated hyaluronic acid (HA-MA) were prepared. An ovine tendon sample was decellularised to obtain the ECM. The study groups of HA, TEN, HA_OS, HA_TEN, and HA_OS_TEN were prepared. The presence of tendon cells was confirmed by picrosirius red staining and the hydrogel scaffolds were analysed using scanning electron microscopy (SEM), swelling, differentiation, compression, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) compatibility analyses. Results The morphology of the samples was analysed by SEM analysis. The HA_OS_TEN sample showed the highest rate of tenogenesis, lowest swelling, high cell viability and differentiation, and optimal compression rates. Conclusion This study showed that hyaluronic acid combined with Ocimum sanctum and tendon ECM is a very good conjugation for the preparation of hydrogel scaffolds for tendon tissue regeneration using MSCs.
ABSTRACT
Addition silicone is a newer type of silicone impression material which has high dimensional stability, accuracy, and flexibility. They are mostly used for fixed restorations such as crowns and bridges. They have high tear strength; however, this strength may be altered upon disinfection. Disinfection of addition silicone prior use is important to reduce the microbial load. The aim of this study is to check whether there is any change in tear strength of addition silicone on disinfection with two commercially available disinfectants. Zhermack Elite HD + Putty material was tested in this study. Base and catalyst material was mixed and the material was cut into strips of dimension 70 mm × 10 mm × 2 mm. Five strips were immersed in sterillium and five strips were immersed in glutaraldehyde solution. These strips were then tested for their tear strength using Instron E3000 Universal Testing Machine. The values obtained were recorded in SPSS software version 22 (IBM SPSS Statistics for Windows, Version 23.0. Armonk, NY: IBM Corp.) for analysis and the P was obtained. The mean tear strength of Group 1, addition silicone immersed in sterillium disinfecting solution was found to be 7.148 ± 2.654 kN/m. The mean tear strength of Group 2, addition silicone immersed in glutaraldehyde disinfectant solution was found to be 7.326 ± 4.062 kN/m. An unpaired Student's t-test was done and P was found to be 0.235. The tear strength values between the groups was statistically not significant. Hence the tear strength of addition silicone was found to be greater in the glutaraldehyde disinfectant solution group.
ABSTRACT
The surface area determination has a vital role in measuring the properties of liquid and solid substances. Fluoride is a potent remineralizing agent of the teeth. The surface area is measured using a goniometer. The main aim of this study is to estimate the wettability of topical fluoride gel on the anterior and posterior teeth surfaces. Topical acidulated phosphate fluoride (APF) gel was taken in small quantities. Anterior and posterior teeth were collected, and their superficial unaltered enamel sections were used as the surface where the contact angle (CA) is determined. The surface wettability is determined using Ossila goniometer to measure CA. Each stage was mounted on a mechanical stage of the goniometer. A calibrated microsyringe was used to dispense drops of the APF gel over the surface of the specimen Group A (anterior tooth cut section) and Group B (posterior tooth cut section). This procedure is repeated for all specimen samples. The mean of CAs of the anterior teeth (88.41°) was greater when compared with the mean of posterior teeth (80.36°) CA. P value found using the paired t-test of SPSS software is found to be 0.271 (>0.05), hence statistically not significant. The greater the CA, the lower is the degree of wettability on the teeth surfaces. The mean CA of the topical fluoride APF gel on the anterior tooth surface is greater when compared to the posterior tooth surface. This signifies that greater wettability is seen on posterior tooth surfaces.
ABSTRACT
Composite is usually a mixture of two phases. The composites used in dentistry have high durability. The individual properties of each phase are important to increase the mechanical property of the composite. This study aimed to determine the surface roughness changes of two composite resin restorative materials after thermocycling. Two of the commercially available composites were chosen (RestoFill and SwissTEC). The composite disks of dimension 10 mm diameter and 3 mm height were prepared using silicone molds, and every increment was light cured for 30 s. A stylus profilometer was used to assess the surface roughness prethermocycling, and then, the disks were subjected to an integrated thermocycler (T.S-4.4) for 1000 cycles. The postsurface roughness was obtained after the thermocycling process using the same stylus profilometer. The surface parameter values before and after thermocycling of the SwissTEC sample is less than that of RestoFill. There was a significant difference between the Rz and Rq values of the two different commercially available composite materials. Thus, the present study concludes that thermocycling influenced the surface roughness of composite resin and increased the surface roughness of both the RestoFill and SwissTEC composites.
ABSTRACT
Glass ionomer cement (GIC) is a material used for restoration in dentistry. The stress in a material right before it yields in a flexure test is referred to as flexural strength. The aim of this study was to evaluate the flexural strength of two different glass ionomers after immersion in fruit juices. Five samples of D-Tech and Shofu GIC were prepared from each for the in vitro study. The eight samples were subjected to a flexural strength process and two were left as control which was followed by the determination of maximum force and displacement, then the collected data were used to determine flexural strength and analyzed using SPSS software (IBM SPSS Statistics for Windows, Version 22.0. Armonk, NY: IBM Corp). The mean value of flexural strength of the D-Tech GIC immersed in fruit juices is 4.4375 and the mean value of Shofu GIC immersed in fruit juices was 8.4975. Paired t-test was done between the two groups and the P value was 0.00, which is considered highly statistically significant. From this study, it is evident that the GIC brand Shofu has higher flexural strength compared to D-Tech. Flexural strength was decreased for both the GIC groups when immersed in grape juice.
ABSTRACT
Glass ionomer cement (GIC) releases fluorides and has good biocompatibility, carbonated drinks, sometimes known as fizzy drinks, are carbonated liquids that contain dissolved carbon dioxide, sweeteners, and natural or artificial flavoring. The aim of our study is to determine the flexural strength of GIC after immersing in carbonated beverages. Twelve samples of GIC filling material were used among which six samples prepared were from the Dtech brand and six were prepared from the Shofu brand for the in vitro study. Bar-shaped specimens were prepared from each group with the dimension of 2 mm × 2 mm × 25 mm. They were immersed in Sprite, fizz drinks, and in distilled water as a control group. The immersion period was 7 days. Then, the determination of maximum force and displacement was done using INSTRON E3000 (ElectroPuls) universal testing machine, then the collected data were used to determine flexural strength. The mean flexural strength of Dtech GIC was 24.84 ± 6.523 Mpa. The mean flexural strength of Dtech GIC was 18.57 ± 11.60 Mpa. The independent sample t-test showed that P = 0.247 (>0.05) which was statistically not significant. The flexural strength of GIC material decreased after being immersed in Sprite and fizz drinks.
ABSTRACT
Surgical sutures are the most well-known surgical biomaterial device for ligating blood vessels. The primary goal of wound closure is to align wound margins to provide a closed and stable environment. Sutures with lesser tensile strength are susceptible to break throughout the healing process due to edema. To evaluate the tensile strength of absorbable and nonabsorbable suture materials after immersion in fruit juices. In this in vitro study, eight samples of commercially available sutures such as black silk and vicryl were divided into two groups: sample -1 were immersed in grape juice and sample -2 were immersed in lemon juice for 1 week. Universal testing machine INSTRON E300 UTM was used to test the tensile strength of various suture materials. The data are statistically analyzed using an independent t-test. The P < 0.05 was considered to be statistically significant. The mean of vicryl suture after immersion in grape and lemon juice was found to be 34.445 and 43.39; the mean value of black silk after immersion in grape and lemon juice was found to be 36.95 and 33.1. The tensile strength of black silk was slightly lower than the vicryl. Independent sample t-test showed that P = 0.561 (>0.05) which is statistically insignificant. Vicryl suture tested to have the highest tensile strength along with excellent knot holding capacity than black silk suture after immersion in fruit juices.
ABSTRACT
Carbonated beverages have side effects on oral health and general health. Flexural strength is a prime mechanical property of restorative material. The aim of this study is to know the effect of carbonated beverages on the flexural strength of composite restorative material. Two types of composites (Ivoclar and Restofill) were used as samples for comparative evaluation of flexural strength. The sample size is 10 where 5 of each type of composites are included. The obtained samples were immersed in carbonated beverages. One sample of each type was used as a control. After 24 h, the flexural strength of all the 10 samples was evaluated using the formula (3FL/2bd2), and the values were tabulated. Average values and graphs were done using SPSS software. The results showed the changes in flexural strength of both Ivoclar and Restofill materials when immersed in carbonated beverages. The mean value of all the samples was taken, and the standard deviation was calculated. After immersion in carbonated liquids, the mean value of Ivoclar and Restofill samples is 58.9 and 35.01, respectively. P = 0.718 (>0.05), which was not statistically significant. After immersion in Coke, the Restofill group exhibits more flexural strength than the Ivoclar group composite; however, the strength reduces when immersed in Sprite as compared to the Ivoclar group composite.
ABSTRACT
Soft denture liners evenly distribute functional loads over denture-bearing tissues. The liners aid in more evenly distributing the pressures of mastication to the underlying tissues by absorbing some of the masticatory forces. The study aimed to evaluate the brushing simulation influence on the surface roughness property of soft-tissue liners. A total of eight samples of Avue brand soft-tissue liners with the composition of varnish base and varnish catalyst were suspended into a standard template extracted and numbered sequentially and surface roughness was calculated using a stylus profilometer. A total of 30,000 cycles brushing were done, where the first group samples were brushed with Colgate toothpaste and the second group brushed with Dabur Red toothpaste using a toothbrush simulator (ZM3.8 SD Mechatronik). The data of both pre- and postbrushing values were recorded manually and statistically uploaded on SPSS software version 22 and values were represented in clustered bar graph forms. The significance value of Ra was 0.321. The significance value of Rq was 0.211. The significance value of Rz was 0.354, hence statistically, insignificant. In the present study, the surface roughness of soft-tissue liners is reduced to a minimal extent after brushing simulation.
ABSTRACT
Resin-based composites have been widely used to reconstruct anterior and posterior teeth, as dentistry has progressed and there has been an increasing interest in the creation of suitable materials to replace damaged tooth tissue. The aim of the study is to compare the effect of fruit juices on the surface roughness of bulk fill and flowable composite restorative material. A total of 8 bulk fill composite and flowable composite resin disc samples were made. These eight samples were tested for surface roughness following immersion in three juices which were pineapple juice, lime juice, orange juice, and distilled water as the control group. They were immersed at room temperature for 7 days. Surface roughness was evaluated by the Mitutoyo SJ-310 contact profilometer. It showed that there was a decrease in the Ra value of flowable composite when compared to bulk fill composite. Rq and Rz values were more for the flowable composite resin group. Flowable composite resin samples had more surface roughness than bulk fill dental composite restorative material after exposure to pineapple, lime, and orange juices.
ABSTRACT
Resin-based composites are increasingly employed in dental restorations due to their esthetics. Flexural strength is the characteristic feature of a substance, and it can be described as the stress present in the material just before it renders in a flexure test. The purpose of this study was to determine the flexural strength of a bulk-fill composite following immersion in fruit juices. Ten samples of bulk-fill type composite filling material were created, with four samples from Restofill (Group 1) and four samples from Ivoclar Vivadent (Group 2). Out of which, four specimens of each brand were included for immersion in fruit juices; two specimens were kept as control. The eight samples were put through a flexural strength test, with four remaining as controls, before being measured for maximum force and displacement on the Instron E3000 universal testing equipment. SPSS version 23 (IBM SPSS Statistics for Windows, Version 23.0. Armonk, NY: IBM Corp.) was used to examine the calculated flexural strength. The composite Ivoclar has a mean flexural strength of 20.52, whereas the composite Restofill has a mean flexural strength of 20.55. The P value for the independent sample t-test between the two groups, Ivoclar and Restofill, was 0.089 (>0.05), which is statistically insignificant. It is clear from this research that the bulk-fill composite resin Ivoclar has a low flexural strength when compared to the sample Restofill after immersion in fruit juices.
ABSTRACT
Flexural strength is the bend strength or stress which causes failure in bending of the material. Composite is the widely used restorative material in the era of esthetic dentistry as it provides better esthetic and mechanical support for both posterior and anterior teeth. The term "thermocycling" refers to the process of changing temperatures in the mouth. Thus, the aim of the study was to determine the effect of thermocycling on flexural strength in two different composite filling materials. Twelve samples of packable type composite filling material, among which four samples prepared were from Restofill l (Group 1) and four were prepared from Ivoclar Vivadent (Group 2) for the study. Bar-shaped composite resin specimens were prepared with dimensions of 2 mm × 2 mm × 25 mm. Then, the eight samples were subjected to a thermocycling process and four left as control. This was followed by the determination of maximum force by a universal testing machine, and then, the collected data were used to determine flexural strength using the formula. The determining flexural strength was analyzed using SPSS version 23.0 by Independent sample t-test. The mean flexural strength of thermocycled Restofill composite filling material is 6.73, whereas the nonthermocycled Restofill sample showed 5.58 and the mean flexural strength of thermocycled Ivoclar Vivadent composite filling material was 5.08. The difference was not statistically significant with P = 0.978 >0.05. The current study concludes that the flexural strength of the Restofill composite (Group 1) was higher than the Ivoclar composite (Group 2). Hence, thermocycling affects the flexural strength of composite resin.
ABSTRACT
Tooth discoloration is considered as one of the most common dental problems among people, and in dentistry, the most common cases are claimed after the restoration. Therefore, in this research, we aim to evaluate the effect of carbonated beverages on the color stability of bulk and flowable composite resin. For the study, 12 composite disc samples were made using the standard dimensions of 10 mm diameter and 2 mm thick. To find the color stability, we used a VITA Easyshade spectrophotometer. We used two different composites of bulk fill and flowable composite resin; the composite brand we used was Tetric ecom plus; as an immersion medium, we used two different carbonated beverages, and the chosen beverages were Appy Fizz and 7Up. 24-h and 7-day postimmersion color stability was evaluated. In the results of postimmersion, we have found the Delta E value for 24 h immersion of flowable and bulk fill composite as 5.8115 and 7.4378, respectively; similarly, the Delta E value for 7 days immersion of flowable and bulk fill composite was 9.9559 and 10.1028, respectively. Using the independent "t"-test, we found that the significance is 0.633 and 0.328, which was statistically not significant. In the present study, when immersed in Appy Fizz juice and 7Up juice, bulk fill composite resins have shown greater discoloration when compared to flowable composite resin material. Thus, the flowable composite resin samples were more color stable.
ABSTRACT
Tooth loss has become one of the most raising issues to be brought up in dentistry, by both patients and dentists. Teeth are considered to be important in case of function and esthetics. Compromise to the dentition would raise social disturbances for the individual. Dental implants are surgical fixtures or artificial tooth roots that are placed into the jawbone. It is one of the best methods of tooth replacement. When considering implants, the dentist must approach it with a multidisciplinary treatment plan. They should consider factors such as age, gender, habit, systemic diseases, oral hygiene, placement site and technique, bone width and bone height, bone density, etc. complications for implant surgery would include bleeding, infections, pain, breakage, etc. A single center retrospective study was done in an institutional setting. The data for the study was retrieved from the college's patient records management software. All patients undergoing implant surgery in the posterior mandibular region were considered. The patients age, gender, implant site, bone width, and bone height were taken into account as variables. The data received was analyzed using SPSS. A total of 737 patients were considered in the study, 383 being male patients and remaining 354 being female patients. the right and left molar teeth were more commonly replaced in comparison to premolars. The association between the primary stability and bone density was statistically significant (p < 0.05). Within the limitations of the study, males had better bone density than females and D2 bone density was predominantly seen in both males and females and between the age group between 26 to 40 years. D2 bone density is seen in both left and right molar regions. Primary stability was associated with the implant length, width and bone density.
