ABSTRACT
An increasing amount of evidence demonstrates the beneficial role of oxytocin (OT) in the cardiovascular system. Similar actions are attributed to genistein, an isoflavonic phytoestrogen. The treatment with genistein activates the OT system in the aorta of ovariectomized (OVX) Sprague-Dawley (SD) rats. The objective of this study was to determine the effects of low doses of genistein on the OT-induced effects in rat hypertension. The hypothesis tested was that treatment of OVX spontaneously hypertensive rats (SHRs) with genistein improves heart structure and heart work through a mechanism involving the specific OT receptor (OTR). OVX SHRs or SD rats were treated with genistein (in microg/g body wt sc, 10 days) in the presence or absence of an OT antagonist (OTA) [d(CH(2))(5), Tyr(Me)(2), Orn(8)]-vasotocin or a nonspecific estrogen receptor antagonist (ICI-182780). Vehicle-treated OVX rats served as controls. RT-PCR and Western blot analysis demonstrated that left ventricular (LV) OTR, downregulated by ovariectomy, increased in response to genistein. In SHRs or SD rats, this effect was blocked by OTA or ICI-182780 administration. The OTR was mainly localized in microvessels expressing the CD31 marker and colocalized with endothelial nitric oxide synthase. In SHRs, the genistein-stimulated OTR increases were associated with improved fractional shortening, decreased blood pressure (12 mmHg), decreased heart weight-to-body weight ratio, decreased fibrosis, and lowered brain natriuretic peptide in the LV. The prominent finding of the study is the detrimental effect of OTA treatment on the LV of SHRs. OTA treatment of OVX SHRs resulted in a dramatic worsening of ejection fractions and an augmented fibrosis. In conclusion, these results demonstrate that cardiac OTRs are involved in the regulation of cardiac function of OVX SHRs. The decreases of OTRs may contribute to cardiac pathology following menopause.
Subject(s)
Cardiomegaly/prevention & control , Genistein/pharmacology , Hypertension/drug therapy , Myocardium/metabolism , Ovariectomy , Receptors, Oxytocin/antagonists & inhibitors , Vasotocin/analogs & derivatives , Ventricular Function, Left/drug effects , Ventricular Remodeling/drug effects , Animals , Atrial Natriuretic Factor/metabolism , Cardiomegaly/etiology , Cardiomegaly/metabolism , Cardiomegaly/physiopathology , Disease Models, Animal , Dose-Response Relationship, Drug , Estradiol/analogs & derivatives , Estradiol/pharmacology , Estrogen Antagonists/pharmacology , Female , Fibrosis , Fulvestrant , Hypertension/complications , Hypertension/metabolism , Hypertension/physiopathology , Myocardial Contraction/drug effects , Myocardium/pathology , Natriuretic Peptide, Brain/metabolism , RNA, Messenger/metabolism , Rats , Rats, Inbred SHR , Rats, Sprague-Dawley , Receptors, Estrogen/antagonists & inhibitors , Receptors, Estrogen/metabolism , Receptors, Oxytocin/genetics , Receptors, Oxytocin/metabolism , Vasotocin/pharmacology , Ventricular Pressure/drug effectsABSTRACT
While an increasing amount of evidence demonstrates the homeostatic functions of the cardiac oxytocin (OT) system, less is known about the role of this hormone in the injured heart. The current study examined the effect of OT infusion on cell apoptosis, expression of proliferating cell nuclear antigen (PCNA) and inflammation in the acute and subacute phases of myocardial infarction (MI). Prior MI male Sprague-Dawley rats were infused subcutaneously with OT 25 or 125 ng/(kg h) for 3 or 7 days. Saline-treated MI and sham-operated rats served as controls. Echocardiography and analysis of cardiac sections were used to disclose OT actions. Left ventricular fractional shortening, estimated to be 46.0 +/- 1.8% in sham controls, declined to 21.1 +/- 3.3% in vehicle-treated MI rats and was improved to 34.2 +/- 2.1 and to 30.9 +/- 2.5% after treatment with OT 25 and 125 ng/(kg h), respectively. OT infusion resulted in: (1) increase of cells expressing PCNA in the infarct zone, diminished cell apoptosis and fibrotic deposits in the remote myocardium; (2) suppression of inflammation by reduction of neutrophils, macrophages and T lymphocytes; (3) depression of the expression of proinflammatory cytokines tumor necrosis factor-alpha and interleukin-6 with promotion of transforming growth factor-beta. OT treatment reduced expression of atrial and brain natriuretic peptides in the infarcted ventricle, as well as the concentration of both peptides in the circulation. These results indicate that continuous OT delivery reduces inflammation and apoptosis in infarcted and remote myocardium, thus improving function in the injured heart.
Subject(s)
Myocardial Infarction/drug therapy , Myocarditis/prevention & control , Oxytocics/therapeutic use , Oxytocin/therapeutic use , Animals , Apoptosis/drug effects , Cytokines/metabolism , Echocardiography , Heart Function Tests , Leukocytes/pathology , Male , Myocardial Infarction/diagnosis , Myocardial Infarction/immunology , Myocardial Infarction/metabolism , Myocarditis/etiology , Myocarditis/metabolism , Myocardium/metabolism , Myocardium/pathology , Natriuretic Peptides/metabolism , Nitric Oxide Synthase Type III/metabolism , Oxytocics/pharmacology , Oxytocin/pharmacology , Proliferating Cell Nuclear Antigen/metabolism , Rats , Rats, Sprague-Dawley , Troponin T/metabolismABSTRACT
BACKGROUND: Cardiopulmonary bypass (CPB), a procedure often used during cardiac surgery, is associated with an inflammatory process that leads to lung injury. We hypothesized that inhaled nitric oxide (INO), which has anti-inflammatory properties, possesses the ability to modulate lung cell apoptosis and prevent CPB-induced inflammation. METHODS: Twenty male pigs were randomly classified into four groups: sham, sham plus INO, CPB, and CPB plus INO. INO (20 ppm) was administered for 24 h after anesthesia. CPB was performed 90 min into INO treatment. BAL fluid and blood were collected at time 0 (before CPB), at 4 h after beginning CPB, and 24 h after beginning CPB (T24). RESULTS: At T(24), BAL interleukin (IL)-8 levels and neutrophil percentages were elevated significantly in the CPB group. At T(24), INO reduced IL-8 concentrations and attenuated the increase of neutrophil percentage in the CPB-plus-INO group. Nitrite-plus-nitrate (NOx) concentrations were decreased significantly in groups without INO. Moreover, animals treated with INO showed higher rates of pulmonary apoptosis compared to their respective control groups except for the sham-plus-INO group, in which they were diminished. CONCLUSION: These results demonstrate that NOx production is reduced after CPB, and that INO acts as an anti-inflammatory agent by decreasing neutrophil numbers and their major chemoattractant, IL-8. INO also increases cell apoptosis in the lungs during inflammatory conditions, which may explain, in part, how it resolves pulmonary inflammation.
Subject(s)
Cardiopulmonary Bypass/adverse effects , Inflammation/drug therapy , Nitric Oxide/therapeutic use , Administration, Inhalation , Animals , Bronchoalveolar Lavage Fluid/cytology , Disease Models, Animal , Inflammation/etiology , Instillation, Drug , Male , Nitric Oxide/administration & dosage , Postoperative Complications/drug therapy , SwineABSTRACT
Nitric oxide (NO) is an important bioregulatory molecule in the nervous, immune and cardiovascular systems. NO participates in the regulation of the daily activities of cells as well as in cytotoxic events. It possesses a controversial effect on cell viability by acting both as a protection against apoptogenic stimuli, or by inducing apoptosis when produced at elevated concentrations. The mechanisms of NO in regulating these biological functions can be either through cyclic guanylate cyclase (cGMP)-dependent or cGMP-independent pathways. The purpose of this review is to highlight the implication of NO in cell signalling, synaptic transmission, and cell death. We focus also on the protective role as well as the toxicity of NO. Finally, the adverse effects of inhaled nitric oxide are also depicted in this review.
