Phytoremediation potential of three terrestrial plant species for removal of atrazine, azoxystrobin, and imidacloprid.

Pesticides can move off-target resulting in contamination of sensitive water bodies and causing adverse effects on inhabiting species. Through best management practices, such as the implementation of vegetative buffer strips, off-target movement of pesticides can be decreased, and compound degradation can be increased via phytoremediation. In this study, blueflag iris (Iris versicolor), broomsedge (Andropogon virginicus) and switchgrass (Panicum virgatum) were planted in soil treated with one of three commonly used pesticides. At 28, 56 and 112 days after treatment (DAT), plants were destructively harvested and analyzed for pesticide residue in soil and above-ground and below-ground vegetation using high-performance liquid chromatography (HPLC). Relative to the amount of pesticide found in planted pots compared to non-planted pots, I. versicolor was found to reduce greater atrazine in soil compared to non-planted pots at 112 DAT by 58.7%. I. versicolor was also the most capable of reducing azoxystrobin, by 86.9% compared to non-planted pots, from the soil at 112 DAT. At the same sampling time, I. versicolor and P. virgatum reduced greater imidacloprid from soil by 62.5% and 64.3% compared to non-planted pots, respectively. This information supports the recommendation for establishment of diverse plant species for optimization of phytoremediation capacities. Novelty statement While research has found that plants can absorb and remediate synthetic chemicals, this practice is only sustainable if used with native plants that require low maintenance and are tolerant to the applied substances. Various previous studies observe plants that are fast-growing, tolerant to environmental conditions, require low-maintenance, and are hardy. However, these plant species are not always suitable for any location and are often considered invasive and/or weed-like. The present research initiates a list of plant species which can be used within the southeastern United States and similar areas to phytoremediate commonly used pesticides atrazine, azoxystrobin, and imidacloprid and prevent off-target movement.

Characterization and Aggressiveness of Take-All Root Rot Pathogens Isolated from Symptomatic Bermudagrass Putting Greens.

Take-all root rot is a disease of ultradwarf bermudagrass putting greens caused by Gaeumannomyces graminis (Gg), Gaeumannomyces sp. (Gx), Gaeumannomyces graminicola (Ggram), Candidacolonium cynodontis (Cc), and Magnaporthiopsis cynodontis (Mc). Many etiological and epidemiological components of this disease remain unknown. Improving pathogen identification and our understanding of the aggressiveness of these pathogens along with growth at different temperatures will advance our knowledge of disease development to optimize management strategies. Take-all root rot pathogens were isolated from symptomatic bermudagrass root and stolon pieces from 16 different golf courses. Isolates of Gg, Gx, Ggram, Cc, and Mc were used to inoculate 'Champion' bermudagrass in an in planta aggressiveness assay. Each pathogen was also evaluated at 10, 15, 20, 25, 30, and 35°C to determine growth temperature optima. Infected plant tissue was used to develop a real-time PCR high-resolution melt assay for pathogen detection. This assay was able to differentiate each pathogen directly from infected plant tissue using a single primer pair. In general, Ggram, Gg, and Gx were the most aggressive while Cc and Mc exhibited moderate aggressiveness. Pathogens were more aggressive when incubated at 30°C compared with 20°C. While they grew optimally between 24.4 and 27.8°C, pathogens exhibited limited growth at 35°C and no growth at 10°C. These data provide important information on this disease and its causal agents that may improve take-all root rot management.

Phytoremediation of azoxystrobin and imidacloprid by wetland plant species Juncus effusus, Pontederia cordata and Sagittaria latifolia.

Azoxystrobin (strobilurin fungicide) and imidacloprid (neonicotinoid insecticide) have been detected in surface waters near treated agricultural, urban, and mixed landscapes. The hazards of pesticide runoff can be prevented through best management practices, including the establishment of diverse wetland plant barriers that can phytoremediate the chemicals in which they come into contact with. In this study, the wetland plant species softrush (Juncus effusus), pickerelweed (Pontederia cordata), and arrowhead (Sagittaria latifolia) were planted in sandy soil containers that were then placed in azoxystrobin or imidacloprid treated water. Every week for 2 months, water samples were collected for pesticide residue analysis using high-performance liquid chromatography (HPLC). At 14, 28, and 56 days after initiation, plants were destructively harvested and analyzed for pesticide residue in soil, above-ground vegetation, and below-ground vegetation. Results from this study report P. cordata reduced greater azoxystrobin (51.7% reduction compared to treated non-planted containers) compared to J. effusus and S. latifolia (24.9% and 28.7% reduction from non-planted containers) at 56 days. However, S. latifolia reduced greater imidacloprid (79.3% reduction compared to non-planted containers) compared to J. effusus and P. cordata (36.0% and 37.1% reduction from non-planted containers) at 56 days.Novelty statement: While research has found that wetland plants can absorb and remediate synthetic chemicals, this practice is only sustainable if used with native plants that require low maintenance and are tolerant to the applied substances. Various previous studies observe plants that are fast-growing, tolerant to environmental conditions, require low-maintenance, and are hardy. However, these plant species are not always suitable for any location and are often considered invasive and/or weed-like. The present research initiates a list of plant species which can be used within the southeastern United States and similar areas to phytoremediate commonly used pesticides azoxystrobin and imidacloprid and prevent off-target movement into sensitive water systems.

Influence of post-application irrigation and mowing timing on fungicide fate on a United States Golf Association golf course putting green.

Fungicides are routinely applied to golf course putting greens throughout the growing season. Gaining a better understanding of fungicide fate can improve fungicide use and stewardship. Therefore, optimizing fungicide applications with post-application management practices may enhance fungicide movement and limit potential off-target effects. Two field studies were initiated on a golf course putting green to evaluate the influence of post-fungicide application irrigation and mowing timing on fungicide movement into the soil profile and removal in turfgrass clippings. Plots were treated with a single application of either pyraclostrobin, triadimefon, or penthiopyrad and received 0.64 cm post-application irrigation immediately or 6 h after application or received no post-application irrigation. Clippings were collected 0, 1, and 3 d after treatment (DAT). Cores were harvested 0, 1, 3, 5, 7, and 14 DAT and dissected into the remaining aboveground vegetation (RAV; verdure/thatch; 0-to-2.5-, 2.5-to-5.1-, and 5.1-to-7.6-cm soil subsections). A small amount of fungicide (<3.6%) was removed with clippings regardless of mowing and irrigation treatment. Post-application irrigation treatment influenced fungicide movement; however, >50% of fungicide remained restricted to the RAV for the first 3 DAT. Less fungicide remained restricted to the RAV, and more fungicide was detected in deeper soil depths when plots were irrigated immediately after application. Fungicide was only detected at the 5.1-to-7.6-cm depth when plots were irrigated immediately. Applying post-application irrigation immediately may result in more fungicide moving down to soilborne targets. Irrigating 6 h after application facilitated moderate fungicide movement compared with irrigating immediately but was better than no post-application irrigation.

Conservation and divergence in sorgoleone production of sorghum species.

Sorgoleone-358 is an important allelochemical of the oily droplets exuded from root hairs of various species in the Sorghum genus. Due to its hydrophobic nature, sorgoleone-358 can be strongly adsorbed onto soil organic matter, resulting in increased sorgoleone soil persistence. Because of the herbicidal activity of sorgoleone on many small-seeded weeds, concerns have been raised that sorghum residues may have a detrimental effect on emergence of wheat used as a double crop in the southeastern United States. Laboratory experiments were conducted to evaluate root exudate production and its sorgoleone-358 content for 36 cultivated sorghum cultivars as well as eight shattercane [Sorghum bicolor (L.) Moench ssp. arundinaceum (Desv.) de Wet & Harlan] accessions and one johnsongrass [S. halepense (L.) Pers.] accession. Using a capillary growing mat system, root exudate was extracted with dichloromethane and subjected to chromatography analysis to determine sorgoleone-358 content. Root biomass of 7- to 12-d-old seedlings averaged 18.8 mg g-1 seed, and root exudate production ranged from 0.2 and 4.8 mg g-1 root fresh weight (RFW). The amount of sorgoleone produced varied greatly among sorghum accessions. Sorgoleone-358 amount in the root exudate averaged 0.5 mg g-1 RFW and varied from 0.13 to 1.05 mg g-1 for shattercane cultivar S7 and cultivated sorghum cultivar 992123, respectively. Regarding volume of root biomass, sorgoleone-358 levels averaged 0.49 mg g-1 (range, 0.06-1.46 mg g-1 ) for sorghum cultivar AAS3479 and shattercane cultivar S2, respectively. Segregation of commercial sorghum cultivars according to their maturity group did not show any difference in root biomass and dry extract production, but early-maturing cultivars produced on average 18% less sorgoleone-358 compared with medium- and late-maturing cultivars. These results suggest that sorgoleone production may be genetically constitutive because sorghum growing conditions were identical across cultivars.

Integration of remote-weed mapping and an autonomous spraying unmanned aerial vehicle for site-specific weed management.

BACKGROUND: Unmanned aerial vehicles (UAVs) have been used in agriculture to collect imagery for crop and pest monitoring, and for decision-making purposes. Spraying-capable UAVs are now commercially available worldwide for agricultural applications. Combining UAV weed mapping and UAV sprayers into an UAV integrated system (UAV-IS) can offer a new alternative to implement site-specific pest management. RESULTS: The UAV-IS was 0.3- to 3-fold more efficient at identifying and treating target weedy areas, while minimizing treatment on non-weedy areas, than ground-based broadcast applications. The UAV-IS treated 20-60% less area than ground-based broadcast applications, but also missed up to 26% of the target weedy area, while broadcast applications covered almost the entire experimental area and only missed 2-3% of the target weeds. The efficiency of UAV-IS management practices increased as weed spatial aggregation increased (patchiness). CONCLUSION: Integrating UAV imagery for pest mapping and UAV sprayers can provide a new strategy for integrated pest management programs to improve efficiency and efficacy while reducing the amount of pesticide being applied. The UAV-IS has the potential to improve the detection and control of weed escapes to reduce/delay herbicide resistance evolution. © 2019 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

A Global Meta-Analysis to Predict Atrazine Sorption from Soil Properties.

Atrazine (2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine) is one of the most widely used herbicides worldwide, and groundwater contamination is of concern, especially in heavily used regions and in edaphic conditions prone to leaching. Soil sorption plays an essential role in atrazine environmental fate, yet consistent atrazine risk prediction remains limited. A quantitative meta-analysis was conducted to characterize the effect of soil properties on atrazine sorption, using 378 previous observations in 48 publications from 1985 to 2015 globally, which included data on soil properties and sorption parameters. A supplemental regional study was conducted to test the derived meta-analysis models. The meta-analysis indicated that percentage organic C (OC) was the most important parameter for estimating atrazine sorption, followed by percentage silt, soil pH, and percentage clay. Meta-analysis and supplemental study models were developed for Freundlich sorption coefficients () and sorption distribution coefficients () as a function of OC. The global meta-analysis models generated positive linear trends for OC with and ( = 0.197 and 0.205, respectively). Organic C was highly correlated with and in supplemental experimental study models ( = 0.93 and 0.92, respectively), indicating accurate prediction of sorption within the evaluated region. Continental models were investigated, which improved the goodness of fit. Models developed via meta-analysis may be used to predict atrazine sorption over wide ranges of data, whereas more accurate and refined prediction can be achieved by specific regional models through experimental studies. However, such models could be improved if standardized agroclimatic conditions, soil classification, and other key variables were more widely reported.

Impact of soil organic carbon on monosodium methyl arsenate (MSMA) sorption and species transformation.

Monosodium methyl arsenate (MSMA), a common arsenical herbicide, is a major contributor of anthropogenic arsenic (As) to the environment. Uncertainty about controls on MSMA fate and the rates and products of MSMA species transformation limits effective MSMA regulation and management. The main objectives of this research were to quantify the kinetics and mechanistic drivers of MSMA species transformation and removal from solution by soil. Laboratory MSMA incubation studies with two soils and varying soil organic carbon (SOC) levels were conducted. Arsenic removal from solution was more extensive and faster in sandy clay loam incubations than sand incubations, but for both systems, As removal was biphasic, with initially fast removal governed by sorption, followed by slower As removal limited by species transformation. Dimethylarsinic acid was the dominant product of species transformation at first, but inorganic As(V) was the ultimate transformation product by experiment ends. SOC decreased As removal and enhanced As species transformation, and SOC content had linear relationships with As removal rates (R2 = 0.59-0.95) for each soil and reaction phase. These results reveal the importance of edaphic conditions on inorganic As production and overall mobility of As following MSMA use, and such information should be considered in MSMA management and regulatory decisions.

Protocols for Quantifying Transferable Pesticide Residues in Turfgrass Systems.

Plant canopies in established turfgrass systems can intercept an appreciable amount of sprayed pesticides, which can be transferred through various routes onto humans. For this reason, transferable pesticide residue experiments are required for registration and re-registration by the United States Environmental Protection Agency (USEPA). Although such experiments are required, limited specificity is required pertaining to experimental approach. Experimental approaches used to assess pesticide transfer to humans including hand wiping with cotton gloves, modified California roller (moving a roller of known mass over cotton cloth) and soccer ball roll (ball wrapped with sorbent strip) over three treated turfgrass species (creeping bentgrass, hybrid bermudagrass and tall fescue maintained at 0.4, 5 and 9 cm, respectively) are presented. The modified California roller is the most extensively utilized approach to date, and is best suited for use at low mowing heights due to its reproducibility and large sampling area. The soccer ball roll is a less aggressive transfer approach; however, it mimics a very common occurrence in the most popular international sport, and has many implications for nondietary pesticide exposure from hand-to-mouth contact. Additionally, this approach may be adjusted for other athletic activities with limited modification. Hand wiping is the best approach to transfer pesticides at higher mowing heights, as roller-based approaches can lay blades over; however, it is more subjective due to more variable sampling pressure. Utility of these methods across turfgrass species is presented, and additional considerations to conduct transferable pesticide residue research in turfgrass systems are discussed.

Comparing Dislodgeable 2,4-D Residues across Athletic Field Turfgrass Species and Time.

2,4-dimethylamine salt (2,4-D) is an herbicide commonly applied on athletic fields for broadleaf weed control that can dislodge from treated turfgrass. Dislodge potential is affected by numerous factors, including turfgrass canopy conditions. Building on previous research confirming herbicide-turfgrass dynamics can vary widely between species, field research was initiated in 2014 and 2015 in Raleigh, NC, USA to quantify dislodgeable 2,4-D residues from dormant hybrid bermudagrass (Cynodon dactylon L. x C. transvaalensis) and hybrid bermudagrass overseeded with perennial ryegrass (Lolium perenne L.), which are common athletic field playing surfaces in subtropical climates. Additionally, dislodgeable 2,4-D was compared at AM (7:00 eastern standard time) and PM (14:00) sample timings within a day. Samples collected from perennial ryegrass consistently resulted in greater 2,4-D dislodgment immediately after application (9.4 to 9.9% of applied) compared to dormant hybrid bermudagrass (2.3 to 2.9%), as well as at all AM compared to PM timings from 1 to 3 d after treatment (DAT; 0.4 to 6.3% compared to 0.1 to 0.8%). Dislodgeable 2,4-D did not differ across turfgrass species at PM sample collections, with ≤ 0.1% of the 2,4-D applied dislodged from 1 to 6 DAT, and 2,4-D detection did not occur at 12 and 24 DAT. In conclusion, dislodgeable 2,4-D from treated turfgrass can vary between species and over short time-scales within a day. This information should be taken into account in human exposure risk assessments, as well as by turfgrass managers and athletic field event coordinators to minimize 2,4-D exposure.

Persistence in and Release of 2,4-D and Azoxystrobin from Turfgrass Clippings.

Research has shown that pesticide residue in clippings from previously treated turfgrass may become bioavailable as grass decomposes, adversely affecting off-target organisms. We conducted a field study to quantify 2,4-D (2,4-dichlorophenoxyacetic acid) and azoxystrobin (methyl(E)-2-{2[6-(2-cyanophenoxy)pyrmidin-4-yloxy]phenyl}-3-methoxyacrylate) residues in turfgrass clippings collected from hybrid bermudagrass [ (L.) Pers. × Burtt-Davy], tall fescue [ (Schreb.) S.J. Darbyshire], and zoysiagrass ( Steud.). A subsequent greenhouse experiment was conducted to measure pesticide release from clippings into water. 2,4-D (1.6 kg a.i. ha) and azoxystrobin (0.6 kg a.i. ha) were applied to field plots at 32, 16, 8, 4, 2, 1, or 0 d before collection of the clippings. Clippings were collected from each experimental unit to quantify pesticide release from clippings into water. Both 2,4-D and azoxystrobin were detected when turfgrass was treated over the 32-d experimental period, suggesting that clipping management should be implemented for an extended period of time after application. Pesticide residue was detected in all water samples collected, confirming 2,4-D and azoxystrobin release from turfgrass clippings; however, pesticide release varied between compounds. Two days after clippings were incorporated in water, 39 and 10% of 2,4-D and azoxystrobin were released from clippings, respectively. Our research supports the currently recommended practice of returning clippings to the turfgrass stand when mowing because removal of 2,4-D and azoxystrobin in clippings may reduce pest control and cause adverse off-target impacts.

Factors Influencing Dislodgeable 2, 4-D Plant Residues from Hybrid Bermudagrass (Cynodon dactylon L. x C. transvaalensis) Athletic Fields.

Research to date has confirmed 2,4-D residues may dislodge from turfgrass; however, experiments have not been conducted on hybrid bermudagrass (Cynodon dactylon L. x C. transvaalensis), the most common athletic field turfgrass in subtropical climates. More specifically, previous research has not investigated the effect of post-application irrigation on dislodgeable 2,4-D residues from hybrid bermudagrass and across turfgrass species, research has been nondescript regarding sample time within a d (TWD) or conducted in the afternoon when the turfgrass canopy is dry, possibly underestimating potential for dislodgement. The effect of irrigation and TWD on 2,4-D dislodgeability was investigated. Dislodgeable 2,4-D amine was reduced > 300% following irrigation. From 2 to 7 d after treatment (DAT), ≤ 0.5% of applied 2,4-D was dislodged from irrigated turfgrass, while ≤ 2.3% of applied 2,4-D was dislodged when not irrigated. 2,4-D dislodgeability decreased as TWD increased. Dislodgeable 2,4-D residues declined to < 0.1% of the applied at 1 DAT- 13:00, and increased to 1 to 3% of the applied 2 DAT- 5:00, suggesting 2,4-D re-suspended on treated turfgrass vegetation overnight. In conclusion, irrigating treated turfgrass reduced dislodgeable 2,4-D. 2,4-D dislodgeability increased as TWD decreased, which was attributed to non-precipitation climatic conditions favoring turfgrass canopy wetness. This research will improve turfgrass management practices and research designed to minimize human 2,4-D exposure.

Management considerations to minimize environmental impacts of arsenic following monosodium methylarsenate (MSMA) applications to turfgrass.

Monosodium methylarsenate (MSMA) is an organic arsenical herbicide currently utilized in turfgrass and cotton systems. In recent years, concerns over adverse impacts of arsenic (As) from MSMA applications have emerged; however, little research has been conducted in controlled field experiments using typical management practices. To address this knowledge gap, a field lysimeter experiment was conducted during 2012-2013 to determine the fate of As following MSMA applications to a bareground and an established turfgrass system. Arsenic concentrations in soil, porewater, and aboveground vegetation, were measured through one yr after treatment. Aboveground vegetation As concentration was increased compared to nontreated through 120 d after initial treatment (DAIT). In both systems, increased soil As concentrations were observed at 0-4 cm at 30 and 120 DAIT and 0-8 cm at 60 and 365 DAIT, suggesting that As was bound in shallow soil depths. Porewater As concentrations in MSMA-treated lysimeters from a 30-cm depth (22.0-83.8 µg L(-1)) were greater than those at 76-cm depth (0.4-5.1 µg L(-1)). These results were combined with previous research to devise management considerations in systems where MSMA is utilized. MSMA should not be applied if rainfall is forecasted within 7 DAIT and/or in areas with shallow water tables. Further, disposing of MSMA-treated turfgrass aboveground vegetation in a confined area - a common management practice for turfgrass clippings - may be of concern due to As release to surface water or groundwater as the vegetation decomposes. Finally, long-term MSMA use may cause soil As accumulation and thus downward migration of As over time; therefore, MSMA should be used in rotation with other herbicides.

Integrated field lysimetry and porewater sampling for evaluation of chemical mobility in soils and established vegetation.

Potentially toxic chemicals are routinely applied to land to meet growing demands on waste management and food production, but the fate of these chemicals is often not well understood. Here we demonstrate an integrated field lysimetry and porewater sampling method for evaluating the mobility of chemicals applied to soils and established vegetation. Lysimeters, open columns made of metal or plastic, are driven into bareground or vegetated soils. Porewater samplers, which are commercially available and use vacuum to collect percolating soil water, are installed at predetermined depths within the lysimeters. At prearranged times following chemical application to experimental plots, porewater is collected, and lysimeters, containing soil and vegetation, are exhumed. By analyzing chemical concentrations in the lysimeter soil, vegetation, and porewater, downward leaching rates, soil retention capacities, and plant uptake for the chemical of interest may be quantified. Because field lysimetry and porewater sampling are conducted under natural environmental conditions and with minimal soil disturbance, derived results project real-case scenarios and provide valuable information for chemical management. As chemicals are increasingly applied to land worldwide, the described techniques may be utilized to determine whether applied chemicals pose adverse effects to human health or the environment.

Arsenic Retention in Foliage and Soil after Monosodium Methyl Arsenate (MSMA) Application to Turfgrass.

Monosodium methyl arsenate (MSMA) is a commonly used herbicide for weed control in turfgrass systems. There is concern that arsenic from applied MSMA could leach to groundwater or run off into surface water, thereby threatening human and ecosystem health. The USEPA has proposed a phase-out of the herbicide but is seeking additional research about the toxicity and environmental impacts of MSMA before establishing a final ruling. Little research has systematically investigated MSMA in field-based settings; instead, risks have been inferred from isolated field measurements or model-system studies. Accordingly, the overall goal of this study was to quantify the fate of arsenic after MSMA application to a managed turfgrass system. After MSMA application to turfgrass-covered and bareground lysimeters, the majority of arsenic was retained in turfgrass foliage and soils throughout year-long experiments, with 50 to 101% of the applied arsenic recovered in turfgrass systems and 55 to 66% recovered in bareground systems. Dissolved arsenic concentrations from 76.2-cm-depth pore water in the MSMA-treated soils were consistently <2 µg L, indistinguishable from background concentrations. As measured by adsorption isotherm experiments, MSMA retention by the sandy soil from our field site was markedly less than retention by a washed sand and a clay loam. Collectively, these results suggest that under aerobic conditions, minimal arsenic leaching to groundwater would occur after a typical application of MSMA to turfgrass. However, repeated MSMA application may pose environmental risks. Additional work is needed to examine arsenic cycling near the soil surface and to define arsenic speciation changes under different soil conditions.