Lamb wave based damage imaging under nonlinear chirp excitation.

Considering a trade-off between temporal-spatial resolution and multi-mode nature of Lamb waves, tone bursts with short durations are usually used as excitations in Lamb wave based damage detection. A short-duration excitation usually requires a large amplitude to carry sufficient energy so as to obtain response signals with enough signal-to-noise ratio and cover a large inspection area. In this paper, an alternative Lamb wave damage imaging method using nonlinear chirp (nonlinear frequency modulation, NLFM) excitation with a long duration and a small amplitude is proposed. The signal processing techniques of pulse compression and dispersion compensation are adopted to compress the long-duration wave packets of response signals into short ones. Compared with conventional tone burst excitations with short durations and small amplitudes, due to the long duration of the nonlinear chirp excitation and the use of pulse compression, sufficient energy can be applied to transducers under small amplitude excitations so the image contrast in imaging will not degrade. Furthermore, as large amplitude excitations are no longer required, high voltage amplifiers are not necessary so the hardware of the Lamb wave testing system is simplified. Experiments on a carbon steel plate with an artificial crack are carried out and Lamb wave signals are collected using a linear array consisting of nine PZTs. Experimental results under the NLFM signal and conventional tone bursts are provided. Experimental results show that under the condition of the same excitation amplitude, the proposed method under the NLFM excitation can achieve better imaging quality compared with methods under conventional tone bursts.

Improvement in the performance of an autoantibody panel in combination with heat shock protein 90a for the detection of early­stage lung cancer.

The early diagnosis of lung cancer is closely associated with the decline of mortality. A panel consisting of seven lung cancer-related autoantibodies (7-AABs) has been shown to be a reliable and specific indicator for the early detection of lung cancer, with a specificity of ~90% and a positive predictive value of ~85%. However, its low sensitivity and negative predictive value limit its wide application. To improve its diagnostic value, the diagnostic efficiencies of 7-AABs in combination with non-specific tumor markers were retrospectively investigated for the detection of early-stage lung cancer. A total of 217 patients with small lung nodules who presented with ground-glass opacity or solid nodules as well as 30 healthy controls were studied. The concentrations of 7-AABs and heat shock protein 90a (HSP90a) were assessed using ELISA. Automated flow fluorescence immune analysis was used for the assessment of CEA, CYFRA21-1, CA199 and CA125 levels. The results showed that 7-AABs + HSP90a possessed a remarkably improved diagnostic efficiency for patients with small pulmonary nodules or for patients with lung nodules of different types, which suggested that 7-AABs in combination with HSP90a could have a high clinical value for the improvement of the diagnostic efficiency of early-stage lung cancer.

Combination of Circulating Cell-Free DNA and Positron Emission Tomography to Distinguish Non-Small Cell Lung Cancer from Tuberculosis.

OBJECTIVE: Non-small cell lung cancer (NSCLC) holds high metabolic tumor burden and circulating cell-free DNA (cfDNA) levels, and the relationship between metabolic tumor burden and cfDNA in NSCLC and the underlying mechanism of their interaction therein remain poorly characterized. Our aim was to evaluate the clinical value of cfDNA and metabolic tumor burden by positron emission tomography-computed tomography (PET/CT) for NSCLC differential diagnosis from tuberculosis in patients with solitary pulmonary nodules. METHODS: Metabolic tumor burden values in humans (subjects with NSCLC, subjects with tuberculosis, and healthy control subjects) and relevant mouse models were detected by preoperative 18F-fluorodeoxyglucose PET (18F-FDG PET/CT) and [3H]-2-deoxy-DG uptake, respectively. The cfDNA levels were detected by quantifying serum cfDNA fragments from the ALU (115 bp) gene using reverse transcription-polymerase chain reaction. RNA sequence was performed to determine the underlying target genes and knocked down or inhibited the target genes in vivo and in vitro to determine the mechanism therein. RESULTS: Metabolic tumor burden correlated with serum cfDNA levels in NSCLC subjects but not in tuberculosis subjects or healthy controls. Mouse models showed a similar phenomenon. In addition, the RNA sequence showed that glucose transporter 1 (GLU1), factor-related apoptosis ligand (FasL), caspase 8, and caspase 3 were significantly increased in NSCLC mouse tumors compared with those in tuberculosis mouse masses. Inhibiting the metabolic tumor burden by blocking or knocking down GLU1 markedly reduced the expression of FasL, the phosphorylation of caspase 8/caspase 3, and serum cfDNA levels/apoptosis percentage in vivo and in vitro. Furthermore, the use of a combination of cfDNA and metabolic tumor burden allowed better ability to distinguish NSCLC subjects from those with tuberculosis or healthy controls than either method used alone. CONCLUSION: Metabolic tumor burden promotes the formation of circulating cfDNA through GLU1-mediated apoptosis in NSCLC, and the combination of cfDNA and metabolic tumor burden could be valuable for distinguishing NSCLC from tuberculosis.

Blood vessel assessment using computed tomography : Effects of ephedrine on uterine artery.

BACKGROUND: Ephedrine increased blood pressure due to the contractile properties of resistance vessels. Excessive contraction of the uterine arteries might cause fetal distress. This study was to determine the diameter of the uterine artery of female New Zealand rabbits after the administration of different doses of ephedrine using CT. METHODS: Thirty-two rabbits were randomly divided into a control group (Group C), low dosage group (Group L), medium dosage group (Group M) and high dosage group (Group H). Normal saline and doses corresponding to the human dose of 7.5, 15 and 30 mg of ephedrine were injected respectively. The marginal ear and uterine artery diameters were measured 5, 10, 15, 30, and 45 min after injection using CT, and the hemodynamic changes were recorded. RESULTS: The increase in mean arterial pressure in group M (p = 0.009), and H (p = 0.013) was higher than that in group C. Compared with group C, substantial contraction of the marginal ear artery was observed at the three doses of ephedrine. There were no differences in the uterine artery diameter among groups L, M and C, However, in Group H, a significant contraction of the uterine artery compared with the other groups (p < 0.001) was observed. DISCUSSION: CT can be used to evaluate the effects of drugs on organs and blood vessels. Ephedrine can not only constrict the peripheral blood vessels but also do not affect the uterine artery at a dose of 15 mg or less. However, the dose should not exceed 30 mg, which may cause severe uterine artery depression.

Characterization of interfacial property of a two-layered plate using a nonlinear low-frequency Lamb wave approach.

This work investigates the feasibility of using a nonlinear low-frequency Lamb wave approach for characterizing the interfacial property of a two-layered plate. Compared with the case of the exact phase-velocity matching, the approximate phase-velocity matching in the low-frequency region can still guarantee the cumulative second-harmonic generation (SHG) of primary Lamb wave propagation, which overcomes the drawbacks arising from the inherent dispersion and multimode features of Lamb wave propagation. For a given two-layered plate, the appropriate mode pair at low frequency consisting of primary Lamb wave and double-frequency Lamb wave (DFLW), which satisfies the approximate phase-velocity matching and nonzero energy flux, is selected to ensure that the amplitude of the generated second harmonic grows within the maximum cumulative distance (MCD). Meanwhile, the numerical analyses indicate that the variation of the SHG efficiency is maximized at the MCD during the interfacial degradation. Using the nonlinear ultrasonic measurement-based experimental setup, the time-domain signal of the second harmonic generated at different propagation distances is conveniently extracted, and then the relative nonlinear acoustic parameter curve consistent with the theoretical prediction is obtained. For examining the influence of interfacial property on the SHG effect of low-frequency Lamb wave propagation, the different annealing cycles of the thin adhesive layer (acrylics) are used to simulate minor changes in the interfacial property of the given two-layered plate. It is found that the relative nonlinear acoustic parameter at the MCD decreases monotonically and sensitively with the increment of annealing cycle number, which verifies the quantitative correlation between the SHG efficiency of low-frequency Lamb wave propagation and the degree of the interfacial degradation. The consistency between the numerical analysis and the experimental measurement shows the potential of using the SHG effect of low-frequency Lamb wave propagation to characterize a minor change in the interfacial properties of a layered composite plate.

Experimental observation of static component generation by Lamb wave propagation in an elastic plate.

The experimental observation of acoustic radiation induced static component (SC) generation by Lamb wave propagation in an elastic plate is first reported. An experimental approach to directly detect the generated SC of primary Lamb wave propagation in an elastic plate is proposed, where a low-frequency wedge transducer is used to detect the SC pulse generated by primary Lamb wave tone-burst that is excited by a high-frequency wedge transducer. The basic requirements are that the group velocity of primary Lamb wave needs to be equal to that of the s0 mode at zero frequency (i.e., the SC), and that the central frequency of the low-frequency wedge transducer needs to be near the center of the main lobe frequency range of the generated SC pulse. For this purpose, the S4 mode whose group velocity is equal to that of the generated SC is selected as the primary Lamb wave, and then the specific mode pair S4-s0 is focused and analyzed. Accompanying propagation of the S4 mode tone-burst, the generated SC pulse detected with the low-frequency wedge transducer is clearly observed. Through respectively measuring the amplitudes of the S4 mode and the generated SC for different spatial separation between the transmitting and receiving wedge transducers, the quantitative relationship of the relative nonlinear acoustic parameter with propagation distance is obtained. The experimental results show that the proposed approach can be used to directly detect the generated SC of Lamb wave propagation, and that the said SC does have a cumulative growth effect with propagation distance.

Modeling and simulation of static component generation of Lamb wave propagation in a layered plate.

Modeling of the acoustic-radiation-induced static component (SC) generation of primary Lamb wave tone burst propagating in a layered plate is conducted. Accompanying the propagation of primary Lamb wave tone burst, there are the finite-duration SC bulk driving force in the interior of the layered plate, and the finite-duration SC traction stress on each surface/interface. According to the modal analysis approach for waveguide excitation, the function of the finite-duration SC bulk driving force and traction stress is to generate the finite-duration SC of primary Lamb wave tone burst. Compared with the second harmonic (SH) generation of Lamb wave propagation in a layered plate, the phase velocity matching is no longer required for the generation of the SC with a cumulative growth effect. Based on modeling of the finite-duration SC generation, it is found that the integrated amplitude of the finite-duration SC generated by propagation of the primary Lamb wave tone burst does grow with propagation distance. Meanwhile, the numerical analyses and the finite element (FE) simulations are conducted to investigate the effect of the said SC generation. It is found that, although the interfacial layer of the layered plate considered is quite thin compared with the upper and lower layers, the numerical analyses indicate that the influence of the interfacial property on the cumulative growth effect of the SC of primary Lamb wave is significant. Furthermore, the FE simulations demonstrate that the cumulative SC of primary Lamb wave tone burst will exhibit a monotonic and sensitive response to the degree of interfacial degradation. This investigation provides an physical insight not previously available into the process of the SC generation of Lamb wave propagation in a layered plate.

Modeling and simulation of frequency mixing response of two counter-propagating Lamb waves in a two-layered plate.

This work investigates modeling of the frequency mixing response (FMR) induced by two counter-propagating Lamb waves with different frequencies in a two-layered plate, and then numerically simulates and analyzes the influences of interfacial properties on the effect of FMR. Based on a perturbation approach and a normal-mode-expansion technique for waveguide excitation, the second-order bulk driving forces and surface/interface stresses at the mixing frequency, originated from the interaction of two counter-propagating Lamb waves within the wave mixing zone, can be regarded as the excitation sources for generation of a series of combined harmonics. It is found that, under the internal resonance condition including the phase matching and nonzero energy flux, the magnitude of the combined harmonic generated increases with increase in the length of mixing zone of the two counter-propagating Lamb waves, and tends to be stable outside the wave mixing zone. Due to the relatively short mixing zone of the two counter-propagating Lamb waves, the effect of FMR has attracted considerable attention because it can enhance the accuracy of location of the local interfacial degradation in the given layered plate. Both the numerical analyses and finite element (FE) simulations performed show that the local interfacial degradation in the two-layered plate may be assessed and located by spatial scanning of wave mixing zone of the two counter-propagating Lamb waves. Through modeling and FE simulations, this paper provides an insight into the physical process of FMR of the two counter-propagating Lamb waves in a two-layered plate, and meanwhile shows a potential for assessment and location of the local interfacial degradation by using the effect of FMR of the two counter-propagating Lamb waves.

A report of clinical diagnosis and treatment of nine cases of coronavirus disease 2019.

Coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 has become an important public health issue in the world. More than 118 000 cases were confirmed around the world. The main clinical manifestations were respiratory symptoms and occasional gastrointestinal symptoms. However, there is no unified standard for the diagnosis and treatment of COVID-19. In the retrospective analysis, we report nine cases of COVID-19, describe the history of contact, clinical manifestations, the course of diagnosis and clinical treatment before, during and after treatment.

Mode pair selection of circumferential guided waves for cumulative second-harmonic generation in a circular tube.

The appropriate mode pairs of primary and double-frequency circumferential guided waves (CGWs) have been investigated and selected for generation of the cumulative second harmonics, which are applicable for quantitative assessment of damage/degradation in a circular tube. The selection criteria follow the requirements: the higher efficiency of cumulative second-harmonic generation (SHG) of primary CGW propagation, and the larger response sensitivity of cumulative SHG to material damage/degradation [characterized by variation in the third-order elastic (TOE) constants]. The acoustic nonlinearity parameter ß of CGW propagation and the change rate of normalized ß versus the TOE constants of tube material are, respectively, used to describe the efficiency of SHG and its response sensitivity to damage/degradation. Based on the selection criteria proposed, all the possible mode pairs of primary and double-frequency CGWs satisfying the phase velocity matching have been numerically examined. It has been found that there are indeed some mode pairs of CGW propagation with the larger values both of ß and the change rate of normalized ß versus the TOE constants. The CGW mode pairs found in this paper are of practical significance for quantitative assessment of damage/degradation in the circular tube.

Assessment of accumulated damage in circular tubes using nonlinear circumferential guided wave approach: A feasibility study.

The feasibility of using the nonlinear effect of primary Circumferential Guided Wave (CGW) propagation for assessing accumulated damage in circular tubes has been investigated. For a given circular tube, an appropriate mode pair of fundamental and double frequency CGWs is chosen to enable that the second harmonic of the primary wave mode can accumulate along the circumferential direction. After the given circular tube is subjected to compression-compression repeated loading for different numbers of loading cycles, the corresponding ultrasonic measurements are conducted. It is found that there is a direct correlation between the acoustic nonlinearity parameter measured with CGWs propagating through one full circumference and the level of accumulated damage in the circular tube. The experimental result obtained validates the feasibility for quantitative assessment of the accumulated damage in circular tubes using the effect of second-harmonic generation by CGW propagation.