ABSTRACT
OBJECTIVE: To explore the effect of cell proliferation, c-met and vascular endothelial growth factor (VEGF) expression by liposomal quercetin in the Eca109/9706 cells induced by liposomal quercetin. METHODS: The suppressing rate of cultured Eca109/9706 cells was detected by MTT assay. After 48-hour-exposure to liposomal quercetin, the expression and cellular localization of c-met and VEGF in Eca109/9706 cells were examined by using immunohistochemistry assay and Western blotting. RESULTS: The suppressing rate of Eca109/9706 cells was in the order of LQ2 group > LQ1 group > nLQ group > control group (P < 0.05). The protein expression of c-met and VEGF was found in Eca109/9706 cells cytoplasma as well as in nuclei by using immunohistochemistry assay. The Immuno-reaction intensity of c-met and VEGF was in the order of control group > nLQ group > LQ1 group > LQ2 group (P <0.05). The western blotting showed the intensity of immunoblotting signals for c-met-IR and VEGF-IR was in the order of control group > nLQ group > LQ1 group > LQ2 group (P < 0.05). CONCLUSION: The liposomal quercetin could suppress the proliferation of culture Eca109/9706 cells, which was about to be related with the suppression high expression of c-met and VEGF.