Electrostatically directed assembly of two-dimensional ultrathin Co2Ni-MOF/Ti3C2Tx nanosheets for electrocatalytic oxygen evolution.

Hydrogen production from water electrolysis is severely restricted by the poor reaction kinetics of oxygen evolution reaction (OER). In this work, a series of two-dimensional (2D) composites MOF/Ti3C2Tx (the MXene phase) were fabricated by electrostatically directed assembly and used as catalysts for OER. The obtained composite materials exhibit enhanced electrocatalytic properties, thanks to the ultrathin 2D/2D heterostructure with abundant active sites in Co2Ni-MOF and the high electronic conductivity of Ti3C2Tx. Among all the catalysts, Co2Ni-MOF@MX-1 achieved the best oxygen evolution performance with the lowest Tafel slope (51.7 mV dec-1) and the lowest overpotential (265 mV on carbon paper) at the current density of 10 mA cm-2. These results demonstrated that the synthesis of 2D composite materials by electrostatically directed assembly could be a feasible and promising method for the preparation of 2D heterostructure catalysts.

Design of an amperometric glucose oxidase biosensor with added protective and adhesion layers.

Enzymes have demonstrated great potential in the development of advanced electroanalysis devices due to their unique recognition and catalytic properties. However, unsatisfactory stability and limited electron communication of traditional enzyme sensors seriously hinder their large-scale application. In this work, a simple and effective method is proposed to improve the stability of enzyme sensors by using sodium hyaluronate (SH) as a protective film, MXene-Ti3C2/Glucose oxidase (GOD) as the reaction layer, and chitosan (CS) /reduced graphene oxide (rGO) as the adhesion layer. Results demonstrate that the repeatability of the designed sensor increased by 73.3% after improving the adhesion between the reaction layer and the current collector and that its response ability was greatly enhanced. Moreover, the long-term stability of the electrode surface with SH protective film proved to be superior than that without protective film, which suggests that this design can effectively improve the overall performance of the enzyme biosensor. This work proposed a multi-tier synergistic approach for improving the reliability of enzyme sensors. Graphical abstract Our proposed protective and adhesion layer can greatly improve the stability of enzyme sensor and realize the rapid detection of glucose in serum sample.

In-situ sludge reduction performance and mechanism in an anoxic/aerobic process coupled with alternating aerobic/anaerobic side-stream reactor.

Activated sludge process with anaerobic side-stream reactors (SR) in the sludge recirculation can achieve in-situ sludge reduction, but sludge reduction efficiency is limited with the low hydraulic retention time (HRT) of SR. An anoxic/aerobic (AO) process, AO coupled with anaerobic SR and AO coupled with alternating aerobic/anaerobic side-stream reactor (AO-OASR) were operated to investigate enhancing effects of alternative aerobic and anaerobic condition (AltOA) in SR on sludge reduction and pollutants removal performance. The AltOA was firstly proposed into SR with a low HRT during the long-term continuous operation. The results showed that AO-OASR presented a lower effluent COD concentration (29.6%) with no adverse effect on nitrogen removal, compared to AO, owing to the intensified refractory carbon reuse in the mainstream aerobic tank. The sludge yield in AO-OASR (0.240 g SS/g COD) was 39.7% lower than that in AO. The OASR accelerated sludge lysis and particle organic matter hydrolysis due to the weakened network strength of flocs, leading to an enhanced increase (17.3 mg/L) of dissolved organic matter (DOM), especially for the fraction of molecular weight (MW) < 25 kDa. The OASR reduced the adenosine triphosphate (ATP) content for heterotrophic anabolism in the mainstream reactor by 42.9%, compared to the ASR. MW < 25 kDa of DOM caused the disturbance of oxidative phosphorylation with a decreasing ATP synthase activity under high-level electronic transport system, leading to ATP dissipation. The cooperation interaction of predator (norank_Chitinophagales), hydrolytic/fermentative bacteria (unclassified_Bacteroidia and Delftia), and slow grower (Trichococcus) played a key role in improving the sludge reduction and carbon reuse in AO-OASR. The results provided an efficient and cost-saving technology for sludge reduction with modified SR under low HRT, which is meaningful to overcome the present bottleneck of deficient reduction efficiency for application in wastewater treatment plants.

Clinical features of hypophosphatemic osteomalacia induced by long-term low-dose adefovir dipivoxil.

OBJECTIVE: To investigate the predictors of hypophosphatemic osteomalacia induced by adefovir dipivoxil (ADV) and to monitor for early detection. PATIENTS AND METHODS: Hospitalized patients who were diagnosed with ADV-related hypo-phosphatemic osteomalacia were recruited and retrospectively analyzed in our hospital from January 2012 to December 2016. A telephone interview was conducted at 1, 3, 6, 9, 12, and 24 months after cessation of ADV. RESULTS: In the 8 patients enrolled in the study, the hypophosphatemic osteomalacia symptoms developed at an average of 5.14 (4-7) years since ADV treatment (10 mg/d). The average alkaline phosphatase (ALP) level was 279.50 (137-548) U/L, which was significantly higher than the normal level (45-125 U/L). The serum phosphorus level was an average of 0.59 (0.43-0.69) mmol/L, which was lower than the normal range (2.06-2.60 mmol/L). Serum calcium levels of the enrolled patients remained within normal limits. Reduced estimated glomerular filtration rate (eGFR <29 mL/min/1.73 m2) was seen in 4 cases. The clinical manifestations were mainly progressive systemic bone and joint pain, frequent fractures, trouble in walking, height reduction (4-6 cm), and so on. After cessation of ADV, symptoms like bone pain resolved gradually. Serum phosphorus level restored to normal in 4.5 months after the withdrawal of ADV. However, in 4 patients, renal function failed to return to normal in 24 months. CONCLUSION: More attention should be paid to the duration of ADV treatment. The level of serum phosphorus and ALP, as well as renal function, should be monitored for early detection of potential adverse drug reactions.