ABSTRACT
OBJECTIVE: Diminished cholesterol efflux activity of apolipoprotein B (apoB)-depleted serum is associated with prevalent coronary artery disease, but its prognostic value for incident cardiovascular events is unclear. We investigated the relationship of cholesterol efflux activity with both prevalent coronary artery disease and incident development of major adverse cardiovascular events (death, myocardial infarction, or stroke). APPROACH AND RESULTS: Cholesterol efflux activity from free cholesterol-enriched macrophages was measured in 2 case-control cohorts: (1) an angiographic cohort (n=1150) comprising stable subjects undergoing elective diagnostic coronary angiography and (2) an outpatient cohort (n=577). Analysis of media from cholesterol efflux assays revealed that the high-density lipoprotein fraction (1.063Subject(s)
Cardiovascular Diseases/epidemiology
, Cholesterol/blood
, Macrophages/metabolism
, ATP Binding Cassette Transporter 1
, ATP-Binding Cassette Transporters/metabolism
, Aged
, Animals
, Apolipoprotein A-I/blood
, Apolipoproteins B/blood
, Biomarkers/blood
, Cardiovascular Diseases/blood
, Cardiovascular Diseases/mortality
, Case-Control Studies
, Cell Line
, Cholesterol, HDL/blood
, Coronary Angiography
, Coronary Artery Disease/blood
, Coronary Artery Disease/epidemiology
, Female
, Humans
, Immunoprecipitation
, Incidence
, Logistic Models
, Male
, Mice
, Middle Aged
, Multivariate Analysis
, Myocardial Infarction/blood
, Myocardial Infarction/epidemiology
, Ohio/epidemiology
, Predictive Value of Tests
, Prognosis
, Proportional Hazards Models
, Risk Assessment
, Risk Factors
, Serum Albumin/metabolism
, Serum Albumin, Human
, Stroke/blood
, Stroke/epidemiology
, Time Factors
