Reduction of Backward Scatterings at the Low-Coherence Kunwu Laser Facility.

We report the first experimental observation on the reduction of backward scatterings by an instantaneous broadband laser with 0.6% bandwidth in conditions of interest for inertial confinement fusion at the low-coherence Kunwu laser facility. The backscatter of stimulated Brillouin scattering (SBS) was robustly reduced by half at intensities of 1-5×10^{14} W/cm^{2} with the 0.53-µm broadband laser in comparison with the monochromatic laser. As SBS dominates energy loss of laser-plasma interactions, the reduction of that demonstrates the enhancement of laser-target coupling by the use of broadband laser. The mitigation of filamentation leads to the reduction of stimulated Raman backscattering at low intensities. In addition, the three-halves harmonic emission was reduced with the broadband laser as well.

Laser cooling by over 7†K in Yb-doped ZBLAN fibers with high-power pumping at atmospheric pressure.

Anti-Stokes fluorescence (ASF) cooling has been demonstrated to be a viable method for balancing the waste heat produced in gain materials. In addition, radiation-balanced fiber lasers and amplifiers at atmospheric pressure have recently been developed. Here, we evaluate the cooling characteristics in a long section of a Yb-doped ZBLAN fiber with high pump power. The fiber has a 200-µm-diameter core and is doped with 3 wt. % Yb3+. As indicated by a thermal camera, cooling by over 7 K below ambient temperature was achieved by core pumping at 1030 nm. The temperature drop distribution at multiple measurement points in the fiber was evaluated with a maximum pump power of tens of watts. The results demonstrate the excellent ASF cooling performance of Yb-doped ZBLAN fibers. This study has great significance for the development of high-power radiation-balanced fiber lasers.

Green synthesis of silica-coated magnetic nanocarriers for simultaneous purification-immobilization of ß-1,3-xylanase.

Tailoring magnetic nanocarriers with tunable properties is of great significance for the development of multifunctional candidate materials in numerous fields. Herein, we report a one-pot biomimetic silicification-based method for the synthesis of silica-coated magnetic nanoparticles. The synthesis process was mild, low cost, and highly efficient, which took only about 21 min compared with 4.5-120 h in other literature. Then, the carriers had been characterized by VSM, SEM, TEM, XRD, FT-IR, and EDS to confirm their function. To evaluate the usefulness of the carriers, they were adopted to couple the purification and immobilization of ß-1,3-xylanase from the cell lysate in a single step with high immobilization yield (92.8 %) and high activity recovery (82.4 %). The immobilized enzyme also retained 58.4 % of the initial activity after 10 cycles and displayed good storage properties, and improved thermal stability, which would be promising in algae biomass bioconversion as well as other diverse applications.

Low coherence laser pulse amplification theory for rare earth ions doped glass medium.

A new theory for the low coherence laser amplification in rare ions doped glass has been proposed. Based on one-dimensional continuous energy level assumption and independent response assumption, the theory can describe the amplification of low coherence laser pulses with any time scale and any bandwidth. By the new theory, McCumber formula can be obtained, and a complete low coherence optical pulse amplification model in neodymium glass is established. Computation shows that at high fluences, inhomogeneous broadening will severely limit energy extraction of narrowband high coherence laser, therefore the extraction of broadband low coherence laser will exceed that of narrowband high coherence laser. In addition, the portion of long-wave of the output spectrum is slightly larger than that predicted by the homogeneous model. The new theory could be beneficial for the studies of low coherence pulse amplification in rare earth doped medium and other laser mediums.

Fast time-evolving random polarization beam smoothing for laser-driven inertial confinement fusion.

We propose a random polarization smoothing method for low-coherence laser to obtain focal spot with random polarization that evolves rapidly in sub-picosecond timescales. Random polarization smoothing is realized by a half-aperture wave plate with sufficient thickness. The degree of polarization and polarization evolution of the focal spot are studied theoretically. The calculation results show that random polarization smoothing can make the polarization of focal spot evolve rapidly and randomly in time and space. Experimentally, the polarization of the focal spot of low-coherence laser with random polarization smoothing is measured by a single-shot polarimeter. The measurement results show that the degree of polarization of the focal spot is reduced to 0.22 on average, which proves the effectiveness of random polarization smoothing. The random polarization smoothing technique on low-coherence laser is expected to reduce the laser plasmas instability through its multi-dimensional random evolution properties.

Full-aperture random polarization smoothing for a low-coherence laser facility.

Two new random polarization smoothing methods using full-aperture elements are proposed on low-coherence lasers, one using birefringent wedge and one using flat birefringent plate. By designing the crystal axis direction and wedge angle of the birefringent plates, the methods can selectively introduce time delay and spatial displacement, so as to obtain fast random evolution of transient polarization by utilizing low spatiotemporal coherence of the laser focal field. Both methods avoid the near field discontinuity and can be used under high fluence. The method using birefringent wedge can slightly improve focal spot uniformity, and the method using flat birefringent plate can obtain non-polarization with DOP lower than 2%. Theoretical studies show that the resulting focal polarization evolves rapidly on sub-picosecond timescales and rapidly covers the entire Poincaré sphere. The method using birefringent wedge is achieved in experiment. The results show that the degree of polarization of the focal spot is reduced from 1 to 0.27, which proves the effectiveness of the full-aperture random polarization smoothing. The full-aperture random polarization smoothing can generate a focal field very close to unpolarized thermal light, which is expected to suppress the laser plasmas instability.

Comparison of mitochondrial genome and development of specific PCR primers for identifying two scuticociliates, Pseudocohnilembus persalinus and Uronema marinum.

BACKGROUND: Pseudocohnilembus persalinus and Uronema marinum (Ciliophora, Scuticociliatia), as parasitic scuticociliatid ciliates, were isolated from Scophthalmus maximus and Takifugu rubripes, respectively, in our previous studies. These ciliates are morphologically very similar; hence, it is difficult to identify specific scuticociliate species using traditional classification methods for performing taxonomic research and disease control studies. METHODS: We annotated the mitochondrial genomes of these two scuticociliates on the basis of previous sequencing, including analyses of nucleotide composition, codon usage, Ka/Ks, and p-distance. We also compared the nucleotide and amino acid similarity of the mitochondrial genomes of P. persalinus, U. marinum, and other 12 related ciliates, and a phylogenetic tree was constructed using 16 common genes. We chose the nad4 and nad7 genes to design specific PCR primers for identification. RESULTS: P. persalinus and U. marinum were found to have a close evolutionary relationship. Although codon preferences were similar, differences were observed in the usage of codons such as CGA, CGC, and GTC. Both Ka/Ks and p-distance were less than 1. Except for yejR, ymf57, ymf67, and ymf75, the amino acid sequence similarity between P. persalinus and U. marinum was greater than 50%. CONCLUSIONS: The mitochondrial genomes of P. persalinus and U. marinum were thoroughly compared to provide a reference for disease prevention and control. The specific PCR primers enabled us to identify P. persalinus and U. marinum rapidly and accurately at the molecular level, thus providing a basis for classification and identification.

High-power, low-coherence laser driver facility.

We report the first (to the best of our knowledge) high-power, low-coherence Nd:glass laser delivering kilojoule pulses with a coherent time of 249 fs and a bandwidth of 13 nm, achieving the 63%-efficiency second-harmonic conversion of the large-aperture low-coherence pulse and good beam smoothing effect. It provides a new type of laser driver for laser plasma interaction and high energy density physics research.

Experiment and theory of beam smoothing using induced spatial incoherence with a lens array.

The smoothing effect of induced spatial incoherence combined with a lens array on a large-bandwidth and short-coherence-time laser is reported. A theoretical model based on statistical optics is developed to describe the spatial and temporal characteristics of the focal spot. Theoretical simulation is consistent with the experimental results. A method was proposed to remove or reduce the residual interference fringes of the experimental focal spot, and both the simulation and analysis show that this method does not affect the smoothing speed of the focal spot.

High-efficiency second-harmonic generation of low-temporal-coherent light pulse.

The nonlinear frequency conversion of low-temporal-coherent light holds a variety of applications and has attracted considerable interest. However, its physical mechanism remains relatively unexplored, and the conversion efficiency and bandwidth are extremely insufficient. Here, considering the instantaneous broadband characteristics, we establish a model of second-harmonic generation (SHG) of a low-temporal-coherent pulse and reveal its differences from the coherent conditions. It is found that the second-harmonic spectrum distribution is proportional to the self-convolution of that of a fundamental wave. Because of this, we propose a method for realizing low-temporal-coherent SHG with high efficiency and broad bandwidth, and experimentally demonstrate a conversion efficiency up to 70% with a bandwidth of 3.1 THz (2.9 nm centered at 528 nm). To the best of our knowledge, this is the highest efficiency and broadest bandwidth of low-temporal-coherent SHG to date. Our research opens the door for the study of low-coherent nonlinear optical processes.

Beam smoothing by a diffraction-weakened lens array combining with induced spatial incoherence.

The smoothing scheme combining a diffraction-weakened lens array with the induced spatial incoherence method is proposed and demonstrated to be an efficient smoothing scheme for broadband laser systems. In our simulation, the RMS illumination nonuniformity of the target spot is reduced to 2% after sufficient smoothing time. The temporal characteristics and spatial power spectral density of the scheme are theoretically analyzed. When the incident light has intensity fluctuations, the uniformity of the target spot is stable, which means a robust smoothing scheme, and which predicts practical applications to the smoothing of broadband laser systems.

The complete mitochondrial genome sequencing of Anisakis simplex isolated from Anoplopoma fimbria.

In this study, zoonotic Anisakis simplex was isolated and identified from the outermost layer of the stomach of diseased Anoplopoma fimbria at an industrial farm in Liaoning, North China (122.1842 E, 39.2616 N). With the completion of A. simplex mitochondrial genome sequencing, the full-length mitochondrial genome of A. simplex was assembled and analyzed. All results indicate that the complete mitochondrial genome of A. simplex was 13,899 bp. There were 20 tRNAs and 12 protein-coding genes (PCGs), and two rRNA all located at the heavy (H) strand. Besides, the phylogenetic tree of 19 A. simplex isolated from different host species was constructed. The results showed that A. simplex isolated from A. fimbria was clustered with Oncorhynchus nerka isolate in a clade. To sum up, our research results would further provide essential data for systematics and evolution study of A. simplex.

Ginkgo biloba Exocarp Extract Inhibits the Metastasis of B16-F10 Melanoma Involving PI3K/Akt/NF-κB/MMP-9 Signaling Pathway.

In recent years, interest in natural plant extracts for cancer treatment is growing in the drug development field. Ginkgo biloba exocarp extract (GBEE) is known for possessing inhibitory effects on various mouse and human cancer cells. And no adverse reactions were observed during its clinical application to cancer patients. The aim of this study is to investigate the inhibitory effect of GBEE on the metastasis of B16-F10 melanoma and its related mechanisms. The B16-F10 melanoma lung metastasis model was established in C57BL/6J mice. It was found that GBEE inhibited the growth and pulmonary metastasis of B16-F10 melanoma transplanted tumor and downregulated the level of MMP-9 protein. Meanwhile, the B16-F10 cells were used to study in vitro. The results showed that GBEE inhibited the proliferation and migration of B16-F10 cells. Simultaneously, it suppressed the heterogeneous adhesion of B16-F10 cells to human umbilical vein endothelial cells (HUVEC) in a concentration-dependent manner. In addition, the levels of p-PI3K, p-Akt, NF-κB, and MMP-9 were decreased, while the PI3K and Akt were not significantly changed. These results indicate that GBEE can inhibit the metastasis of B16-F10 melanoma via multiple links and the molecular mechanism involved the regulation of PI3K/Akt/NF-κB/MMP-9 signaling pathway.

Mitochondrial genome sequencing and analysis of scuticociliates (Uronema marinum) isolated from Takifugu rubripes.

Scuticociliates are dangerous parasitic pathogens for in worldwide mariculture. Scuticociliates cause high mortality to marine fish. After an outbreak of scuticociliatosis in Takifugu rubripes in Liaoning Province, northern China, Uronema marinum, a scuticociliate, was identified. In this study, using Illumina MiSeq next-generation sequencing, the mitochondrial genome of U. marinum was assembled and analysed phylogenetically using mitochondrial genomes of other scuticociliates. The complete U. marinum mitochondrial genome was 39,845 bp; it contained two rRNAs, six tRNAs, and 39 protein-coding genes (PCGs). From the 39 PCGs, 15 PCGs were located on the heavy strand, and 24 PCGs on the light strand of U. marinum mitogenome. The phylogenetic tree showed that there were two main clades, Oligohymenophorea and Spirotrichea. Nine ciliate species were clustered together within Oligohymenophorea; Uronema marinum was a separate cluster sharing a relatively close ancestry with Hymenostomatida. The results of this study will help advance the systematics, and studies of evolution and molecular epidemiology of scuticociliates.

Mitochondrial genome sequencing of notorious scuticociliates (Pseudocohnilembus persalinus) isolated from Turbot (Scophthalmus maximus L.).

Previously, a pathogenic ciliate was isolated from the surface ulcer of a diseased Turbot (Scophthalmus maximus L.) at an aquaculture farm in North China. After morphological and molecular biological identification based on 18rRNA, the ciliated was identified as the notorious scuticociliates (Pseudocohnilembus persalinus). In this study, the whole sequence of the mitochondrial genomic gene of P. persalinus was carried out. The sequencing results showed that the complete sequence of P. persalinus mitogenome was 38,375 bp. There were 2 rRNAs, 4 tRNAs, and 34 protein-coding genes (PCGs), respectively, located on both the heavy strand and the light stand. 15 PCGs were on the heavy strand, and 19 PCGs on the light strand. Besides, phylogenetic trees among 11 ciliates were constructed based on the sequences of 17 PCGs located in mitogenome using BI methods. The results of clustering showed that P. persalinus and Uronema marinum was the first cluster belonging to the order Scuticociliatida. Our research results will further provide primary data for evolution and classified study of scuticociliates.

Laser-damage characteristics of Nd:glass active mirrors.

The active-mirror architecture is widely used in high-power laser systems. In this study, the laser-damage characteristics of Nd:glass active mirrors are investigated. They are exposed to nanosecond 1064 nm laser incident from the Nd:glass. The laser-induced damage thresholds (LIDTs) of the coated sides are lower than those of the uncoated sides. The LIDT of the active mirror whose substrate is manually scrubbed is lower than that of one whose substrate is ultrasonically cleaned. Analysis shows that the absorbing surface defects on the manually scrubbed Nd:glass surface are responsible for the lower LIDT of the active mirror prepared via manual scrubbing, while the subsurface defects in the ultrasonically cleaned Nd:glass substrate are the main reason for the damage of the active mirror prepared via ultrasonic cleaning. The strong standing-wave electric field near the interface between the coating and the Nd:glass substrate is another factor affecting the damage of the active mirror.

Ginkgo biloba exocarp extracts induces apoptosis in Lewis lung cancer cells involving MAPK signaling pathways.

ETHNOPHARMACOLOGICAL RELEVANCE: A fruit of Ginkgo biloba L. is known as Ginkgo nuts. It is an edible traditional Chinese medicine, and could be used for the treatment of cancer thousands of years ago in China. The extracts prepared from the exocarp of Ginkgo biloba (Ginkgo biloba exocarp extracts, GBEE) has the effects of anti-cancer, immune promotion, anti-aging and etc. AIM OF STUDY: To study the effects of GBEE inducing apoptosis in Lewis lung cancer (LLC) cells and the role of Mitogen-activated protein kinase(MAPK) signaling pathways in it. MATERIALS AND METHODS: The LLC solid tumor model was established in C57BL/6J mice. The tumor-bearing mice were randomly divided into 5 groups. A normal control group without tumor cells was established additionally. There were 10 mice in each group, and they were dosed 24h after inoculation. The GBEE (50, 100, 200mg/kg b.w.) groups were dosed by intragastric gavage (i.g.). The mice in positive control group were intraperitoneal (i.p.) injected with cyclophosphamide (CPA) at a dose of 20mg/kg (b.w.). The model control group and the normal control group were both given normal saline (NS) by i.g.. All the groups were dosed at a volume of 0.1mL/10g (b.w.), once a day for 18d. The day after the last administration, the transplanted tumors was stripped and weighed, and the inhibition rate was calculated. In vitro experiments, MTT method was applied to detect the effects of GBEE on LLC cells and primary cultured mouse lung cells. Annexin V-FITC/PI method was used to detect the apoptosis rate of LLC cells. Rhodamine 123 method was used to detect the Mitochondrial transmembrane potential (MTP). Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to detect the levels of Fas mRNA. Western Blot was used to detect the expression of Bax, Bcl-2, Cyt C, cleaved Caspase-3 and MAPK proteins in the corresponding parts of LLC cells. RESULTS: GBEE (50-200mg/kg) inhibited the growth of LLC transplanted tumors with a dose-effect relationship. GBEE (5-160µg/mL) inhibited the proliferation of LLC cells in vitro with the half maximal inhibitory concentration (IC50) value of 162.43µg/mL, while it had no significant inhibitory effects on the primary cultured mouse lung cells. After GBEE (10, 20 and 40µg/mL) acted on the LLC cells, the apoptosis rate was increased and the MTP was decreased. The ratio of Bax/Bcl-2 was increased in the cells. Meanwhile, it also promoted the translocation of Bax/Bcl-2 in mitochondrial membrane and the release of Cyt C from mitochondria to cytosol. In addition, it up-regulated the cleaved-Caspase-3 protein expression. The mRNA levels of Fas and the protein levels of Fas, FasL and p-p38 in the cells were both increased. The levels of p-ERK1/2 and p-JNK1/2 protein were down-regulated but the p38, ERK1/2 and JNK1/2 were not significantly changed. CONCLUSIONS: GBEE induces apoptosis in LLC cells via mitochondrial-mediated intrinsic pathway and death receptor-mediated extrinsic pathway, which may be closely relevant to the regulation of MAPK signaling pathways.

Spectral phase effects and control requirements of coherent beam combining for ultrashort ultrahigh intensity laser systems.

Based on the premise that further improvements to the size and damage threshold of large-aperture optical components are severely limited, coherent beam combining (CBC) is a promising way to scale up the available peak power of pulses for ultrashort ultrahigh intensity laser systems. Spectral phase effects are important issues and have a significant impact on the performance of CBC. In this work, we analyze systematically factors such as spectral dispersions and longitudinal chromatism, and get the general spectral phase control requirements of CBC for ultrashort ultrahigh intensity laser systems. It is demonstrated that different orders of dispersion influence intensity shape of the combined beam, and high-order dispersions affect the temporal contrast of the combined beam, while the number of the channels to be combined has little impact on the temporal Strehl ratio (SR) of the combined beam. In addition, longitudinal chromatism should be controlled effectively since it has a detrimental effect on the combined beam at the focal plane, both temporally and spatially.

Investigation of phase effects of coherent beam combining for large-aperture ultrashort ultrahigh intensity laser systems.

Large-aperture ultrashort ultrahigh intensity laser systems are able to achieve unprecedented super-high peak power. However, output power from a single laser channel is not high enough for some important applications and it is difficult to improve output power from a single laser channel significantly in the near future. Coherent beam combining is a promising method which combines many laser channels to obtain much higher peak power than a single channel. In this work, phase effects of coherent beam combining for large-aperture ultrashort laser systems are investigated theoretically. A series of numerical simulations are presented to obtain the requirements of spatial phase for specific goals and the changing trends of requirements for different pulse durations and number of channels. The influence of wavefront distortion on coherent beam combining is also discussed. Some advice is proposed for improving the performance of combining. In total, this work could help to design a practical large-aperture ultrashort ultrahigh intensity laser system in the future.

Investigations on the catastrophic damage in multilayer dielectric films.

HfO2/SiO2 coatings are always fluence-limited by a class of rare catastrophic failures induced by a nanosecond laser with a wavelength of 1053 nm. The catastrophic damage in HfO2/SiO2 coatings behaves as the damage growth with repeated laser irradiation, and thus eventually limits the mirror performance. Understanding the damage processes and mechanisms associated with the catastrophic damage are important for reducing the occurrence of the catastrophic failure and allowing the HfO2/SiO2 coatings to survive at the high fluence required by high laser systems. The rough damage behavior of the catastrophic failure at the proper critical fluence is present. The pit and delamination in the catastrophic failure are investigated to find the possible reasons leading to the catastrophic failure. The experimental results indicate that nodular defect originated from the substrate easily incurs the catastrophic damage. The electric field enhancements of the pit and the substrate impurities may contribute to this phenomenon. The delamination is always present on the left of the pit when laser irradiates from left to right at oblique incidence, which may be related to the plasma plume toward the laser incidence.