Pollutants removal and connections among sludge properties, metabolism potential and microbial characteristics in aerobic granular sequencing batch reactor for petrochemical wastewater treatment.

Petrochemical wastewater contains inhibitory compounds such as aromatics that are toxic to microorganisms during biological treatment. The compact and layered structure and the high amount of extracellular polymeric substances (EPS) in aerobic granular sludge (AGS) can contribute to protecting microorganisms from the harsh environment. This study evaluated the changes in the granule properties, pollutants removal, microbial metabolic potential and molecular microbial characteristics of the AGS process for petrochemical wastewater treatment. Granules treating petrochemical wastewater had a higher SVI30/SVI5 value (0.94) than that treating synthetic wastewater. An increase in bioactivity and EPS secretion with higher bio-polymer composition, specifically the functional groups such as hydroxyl, alkoxy and amino in protein, was observed, which promoted biomass aggregation. The granules also had more than 2-fold higher specific oxygen utilization rate. The AGS-SBR process obtained an average COD removal of 93% during petrochemical wastewater treatment and an effluent bCOD of below 1 mg L-1. No obvious inhibition of nitrification and denitrification activity was observed in the processes attributed to the layered structure of AGS. The average effluent NH4+-N of 5.0 mg L-1 was obtained and TN removal efficiencies of over 80.0% was achieved. Molecular microbial analysis showed that abundant functional genera Stenotrophomonas and Pseudoxanthomonas contributed to the degradation of aromatics and other petroleum organic pollutants. They were enriched with the variation of group behavior while metabolisms of amino acids and carboxylic acids by the relevant functional genera (e.g., Cytophagia) were significantly inhibited. The enrichment of Flavobacterium and Thermomonas promoted nitrification and denitrification, respectively. This research revealed the rapid start-up, enhanced granule structural strength, high inhibition resistance and considerable performance of AGS-SBR for petrochemical wastewater treatment.

Periodic step polarization accelerates electron recovery by electroactive biofilms (EABs).

Relatively low rate of electron recovery is one of the factors that limit the advancement of bioelectrochemical systems. Here, new periodic polarizations were investigated with electroactive biofilms (EABs) enriched from activated sludge and Geobacter sulfurreducens biofilms. When representative anode potentials (Ea ) were applied, redox centers with midpoint potentials (Emid ) higher than Ea were identified by localized cyclic voltammetry. The electrons held by these redox centers were accessible when Ea was raised to 0.4 V (vs. Ag/AgCl). New periodic polarizations that discharge at 0.4 V recovered electrons faster than normal periodic and fixed-potential polarizations. The best-performing periodic step polarization accelerated electron recovery by 23%-24% and 12%-76% with EABs and G. sulfurreducens biofilms, respectively, compared to the fixed-potential polarization. Quantitative reverse transcription polymerase chain reaction showed an increased abundance of omcZ mRNA transcripts from G. sulfurreducens after periodic step polarization. Therefore, both the rate of energy recovery by EABs and the performance of bioelectrochemical systems can be enhanced by improving the polarization schemes.

Optimization of biofilm conductance measurement with two-electrode microbial electrochemical cells (MECs).

This study was conducted to develop a standardized and consistent method for biofilm conductance measurement for an improved comprehension of extracellular electron transfer. Biofilm conductance (2.12 ± 0.25 × 10-4 S) with and without a fixed anode potential did not show significant difference. The conductance showed a sigmoidal relationship with anode potential. The current-voltage profile of the tested biofilm at applied voltage larger than 100 mV showed deviation from Ohm's law. Up to 69% decrease in biofilm conductance and deviation from Ohm's law were observed in the current-voltage profile when the measurement time increased. By choosing the voltage range (0- 100 mV) and step (25 mV), measurement time (100-s at each voltage step), and anode control mode, these operation settings were found more suitable for consistent and accurate biofilm conductance measurement in the 2-Au MEC system. This represents the first study that comprehensively evaluated the environmental and instrumental parameters for biofilm conductance measurement.

A critical review of wastewater quality variation and in-sewer processes during conveyance in sewer systems.

In-sewer physio-biochemical processes cause significant variations of wastewater quality during conveyance, which affects the influent to a wastewater treatment plant (WWTP) and arguably the microbial community of biological treatment units in a WWTP. In wet weather, contaminants stored in sewer deposits can be resuspended and migrate downstream or be released during combined sewer overflows to the urban water bodies, posing challenges to the treatment facilities or endangering urban water quality. Therefore, in-sewer transformation and migration of contaminants have been extensively studied. The compiled results from representative research in the past few decades showed that biochemical reactions are both cross-sectionally and longitudinally organized in the deposits and the sewage, following the redox potential as well as the sequence of macromolecule/contaminant degradation. The sewage organic contents and sewer biofilm microorganisms were found to covary but more systematic studies are required to examine the temporal stability of the feature. Besides, unique communities can be developed in the sewage phase. The enrichment of the major sewage-associated microorganisms can be explained by the availability of biodegradable organic contents in sewers. The sewer deposits, including biofilms, harbor both microorganisms and contaminants and usually can provide longer residence time for in-sewer transformation than wastewater. However, the interrelationships among contaminant transformation, microorganisms in the deposits/biofilms, and those in the sewage are largely unclear. Specifically, the formation and migration of FOG (fat, oil, and grease) deposits, generation and transport of contaminants in the sewer atmosphere (e.g., H2S, CH4, volatile organic compounds, bioaerosols), transport and transformation of nonconventional contaminants, such as pharmaceuticals and personal care products, and wastewater quality variation during the biofilm rehabilitation period after damages caused by rains/storms are some topics for future research. Moreover, systematic and standardized field analysis of real sewers under dynamic wastewater discharge conditions is necessary. We believe that an improved understanding of these processes would assist in sewer management and better prepare us for the challenges brought about by climate change and water shortage.

Early phase effects of silicate and orthophosphate on lead (Pb) corrosion scale development and Pb release.

Orthophosphate is widely used to control lead (Pb) release in drinking water distribution systems, but phosphorus addition is not sustainable. Alternative corrosion control treatments are needed, and sodium silicate is one possibility. Here, pre-corroded Pb coupons-with and without free chlorine-were used to examine early-phase corrosion scale development after silicate addition, with orthophosphate as a reference corrosion inhibitor. Scale development was evaluated in terms of total Pb release, phase transformation, electrochemical impedance, morphological changes, Pb dissolution kinetics, and short-term Pb-Cu galvanic corrosion. Elevated Pb release occurred for approximately one month after silicate addition, and total Pb release peaked at 1968.1 µg/L and 1176.9 µg/L from systems with and without free chlorine, respectively. In contrast, orthophosphate-treated coupons exhibited fewer, less pronounced spikes in Pb release. By day 354, the median total Pb release from orthophosphate-treated coupons with and without free chlorine had decreased to 3.7 and 5.0 µg/L, respectively, while the median total Pb release from corresponding silicate-treated coupons was much higher, at 44.9 µg/L and 34.3 µg/L. Calcium lead apatite (Ca0.56Pb3.77(PO4)3OH0.67) was identified in orthophosphate-treated scales, with hydroxylpyromorphite (Pb5(PO4)3OH) present in the absence of free chlorine. Plattnerite occurred on coupons in all chlorinated systems. Pb silicate compounds were not detected, but Ca2SiO4 and Na2Ca2(SiO3)3 were identified by X-ray powder diffraction. The charge transfer: film resistance ratio characterizing the orthophosphate-treated coupons decreased slowly while that of the silicate-treated coupons increased after silicate was added. These variations suggest orthophosphate provided better corrosion control than silicate did. Silicate treatment generally caused degradation of the top Pb scale layer, resulting in elevated Pb release, while orthophosphate encouraged the growth of more structured, generally thicker, corrosion scales that were effective in limiting Pb release.

Induction of cathodic voltage reversal and hydrogen peroxide synthesis in a serially stacked microbial fuel cell.

We developed an innovative strategy to address the inhibition of anode-respiring bacteria due to voltage reversal in serially stacked microbial fuel cells by inducing cathodic voltage reversal and H2O2 production. When platinum-coated carbon (Pt/C) cathodes were employed (stacked MFCPt/C) and the MFC was operated with acetate medium, the last unit (MFC 4) caused a voltage reversal of -0.8 V with a substantial anode overpotential of 1.22 V. After replacing the Pt/C cathode with a Pt-free carbon gas diffusion electrode in MFC 4, an electrode overpotential, approximately 0.5 V, was shifted from the anode to the cathode, inducing cathodic voltage reversal. Under cathodic voltage reversal, MFC 4 generated H2O2 at a production rate of 117 mg H2O2/m2-h. Hence, under cathodic voltage reversal induced by Pt-free cathodes, due to less anode polarization, the anode-respiring activity can largely be sustained in a stacked MFC that treats organic wastewater consistently and the quality of treated wastewater may be improved with energy-efficient and on-site generated H2O2.

Manganese Increases Lead Release to Drinking Water.

Lead and manganese are regulated in drinking water due to their neurotoxicity. These elements have been reported to co-occur in drinking water systems, in accordance with the metal-scavenging properties of MnO2. To the extent that manganese is a driver of lead release, controlling it during water treatment may reduce lead levels. We investigated transport of lead and manganese at the tap in a full-scale distribution system: consistent with a cotransport phenomenon, the two metals were detected in the same colloidal size fraction by size-exclusion chromatography with multielement detection. We also studied the effect of manganese on lead release using a model distribution system: increasing manganese from 4 to 215 µg L-1 nearly doubled lead release. This effect was attributed primarily to deposition corrosion of lead by oxidized phases of manganese, and we used 16S rRNA sequencing to identify bacteria that may be relevant to this process. We explored the deposition corrosion mechanism by coupling pure lead with either MnO2-coated lead or pure lead exposed to MnO2 in suspension; we observed galvanic currents in both cases. We attributed these to reduction of Mn(IV) under anaerobic conditions, and we attributed the additional current under aerobic conditions to oxygen reduction catalyzed by MnO2.

Understanding the Impact of Extracellular Polymeric Substances on Lead Release in Drinking Water Systems.

Lead release in a lead (Pb, anode)-iron oxide (α-Fe2O3, cathode) galvanic system was studied under the influence of synthetic extracellular polymeric substances (sEPS). Sodium alginate, bovine serum albumin (BSA), and cytochrome c represented extracellular polysaccharides, proteins, and electrochemically active components, respectively. Microbiologically influenced corrosion was investigated using sEPS and pelleted and resuspended Pseudomonas aeruginosa cells. Relative to the anaerobic inorganic control, Pb release increased by 156, 202, and 198 µg/L when sEPS was present on the cathode side at 200 mg/L (100 mg/L alginate + 100 mg/L BSA), 400 mg/L (200 mg/L alginate + 200 mg/L BSA), and 200 mg/L with 123.84 mg/L cytochrome c, respectively, under anaerobic conditions. When the cathode was aerated, Pb release increased by 75, 260, and -71 µg/L under the aforementioned conditions, all relative to the aerated inorganic control. When sEPS was instead present on the anode side, sEPS caused localized corrosion on Pb and resulted in higher Pb release than predicted by electric current. P. aeruginosa generally enhanced corrosion; when cells were dosed in the anode side, part of the oxidized Pb was immobilized by cells or organic compounds adhered to the electrodes.

Anaerobic oxidation of methane coupled with extracellular electron transfer to electrodes.

Anaerobic oxidation of methane (AOM) is an important process for understanding the global flux of methane and its relation to the global carbon cycle. Although AOM is known to be coupled to reductions of sulfate, nitrite, and nitrate, evidence that AOM is coupled with extracellular electron transfer (EET) to conductive solids is relatively insufficient. Here, we demonstrate EET-dependent AOM in a biofilm anode dominated by Geobacter spp. and Methanobacterium spp. using carbon-fiber electrodes as the terminal electron sink. The steady-state current density was kept at 11.0 ± 1.3 mA/m2 in a microbial electrochemical cell, and isotopic experiments supported AOM-EET to the anode. Fluorescence in situ hybridization images and metagenome results suggest that Methanobacterium spp. may work synergistically with Geobacter spp. to allow AOM, likely by employing intermediate (formate or H2)-dependent inter-species electron transport. Since metal oxides are widely present in sedimentary and terrestrial environments, an AOM-EET niche would have implications for minimizing the net global emissions of methane.

Quantification of the methane concentration using anaerobic oxidation of methane coupled to extracellular electron transfer.

A biofilm anode acclimated with growth media containing acetate, then acetate+methane, and finally methane alone produced electrical current in a microbial electrochemical cell (MxC) fed with methane as the sole electron donor. Geobacter was the dominant genus for the bacterial domain (93%) in the biofilm anode, while methanogens (Methanocorpusculum labreanum and Methanosaeta concilii) accounted for 82% of the total archaeal clones in the biofilm. Fluorescence in situ hybridization (FISH) imaging clearly showed a biofilm of mixed bacteria and archaea, suggesting a syntrophic interaction between them for performing anaerobic oxidation of methane (AOM) in the biofilm anode. Measured cumulative coulombs were linearly correlated to the methane-gas concentration in the range of 10-99.97% (R2≥0.99) when the measurement was sustained for at least 50min Thus, cumulative coulombs over 50min could be used to quantify the methane concentration in gas samples.

Microbial fuel cells as discontinuous portable power sources: syntropic interactions with anode-respiring bacteria.

For microbial fuel cells (MFCs) to work as portable power sources used in a discontinuous manner, anode-respiring bacteria (ARB) should survive for at least several days in the absence of exogenous electron donors, and immediately generate current upon addition of an electron donor. Here, we proved that biopolymer-accumulating bacteria provide substrate (fuel) for ARB to generate current in lack of exogenous electron donor in 4 days, which allows MFCs to be used as portable power sources.

Syntrophic interactions between H2-scavenging and anode-respiring bacteria can improve current density in microbial electrochemical cells.

High current density of 10.0-14.6A/m(2) and COD removal up to 96% were obtained in a microbial electrochemical cell (MEC) fed with digestate at hydraulic retention time (HRT) of 4d and 8d. Volatile fatty acids became undetectable in MEC effluent (HRT 8d), except for trivial acetate (4.16±1.86mgCOD/L). Accumulated methane only accounted for 3.42% of ΔCOD. Pyrosequencing analyses showed abundant fermenters (Kosmotoga spp.) and homoacetogens (Treponema spp.) in anolytes. In anode biofilm, propionate fermenters (Kosmotoga, and Syntrophobacter spp.), homoacetogens (Treponema spp.), and anode-respiring bacteria (ARB) (Geobacter spp. and Dysgonomonas spp.) were dominant. These results imply that syntrophic interactions among fermenters, homoacetogens and ARB would allow MECs to maintain high current density and coulombic efficiency.

Separation of competitive microorganisms using anaerobic membrane bioreactors as pretreatment to microbial electrochemical cells.

Anaerobic membrane bioreactors (AnMBRs) as pretreatment to microbial electrochemical cells (MECs) were first assessed for improving energy recovery. A dual-chamber MEC was operated at hydraulic retention time (HRT) ranging from 1 to 8d, while operating conditions for an AnMBR were fixed. Current density was increased from 7.5 ± 0 to 14 ± 1A/m(2) membrane with increasing HRT. MEC tests with AnMBR permeate (mainly propionate and acetate) and propionate medium confirmed that propionate was fermented to acetate and hydrogen gas, and anode-respiring bacteria (ARB) utilized these fermentation products as substrate. Membrane separation in the AnMBR excluded fermenters and methanogens from the MEC, and thus no methane production was found in the MEC. The lack of fermenters, however, slowed down propionate fermentation rate, which limited current density in the MEC. To symphonize fermenters, H2-consumers, and ARB in biofilm anode is essential for improving current density, and COD removal.

Adsorption of clofibric acid and ketoprofen onto powdered activated carbon: effect of natural organic matter.

The adsorption of two acidic pharmaceutically active compounds (PhACs), clofibric acid and ketoprofen, onto powdered activated carbon (PAC) was investigated with a particular focus on the influence of natural organic matter (NOM) on the adsorption of the PhACs. Suwannee River humic acids (SRHAs) were used as a substitute for NOM. Batch adsorption experiments were conducted to obtain adsorption kinetics and adsorption isotherms with and without SRHAs in the system. The adsorption isotherms and adsorption kinetics showed that the adsorption ofclofibric acid was not significantly affected by the presence of SRHAs at a concentration of 5 mg (as carbon) L(-1). An adsorption capacity of 70 to 140 mg g(-1) was observed and equilibrium was reached within 48 h. In contrast, the adsorption of ketoprofen was markedly decreased (from about 120 mg g(-1) to 70-100 mg g(-1)) in the presence of SRHAs. Higher initial concentrations of clofibric acid than ketoprofen during testing may explain the different behaviours that were observed. Also, the more hydrophobic ketoprofen molecules may have less affinity for PAC when humic acids (which are hydrophilic) are present. The possible intermolecular forces that could account for the different behaviour of clofibric acid and ketoprofen adsorption onto PAC are discussed. In particular, the relevance of electrostatic forces, electron donor-acceptor interaction, hydrogen bonding and London dispersion forces are discussed