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1.
Mikrobiologiia ; 72(1): 131-5, 2003.
Article in Russian | MEDLINE | ID: mdl-12698804

ABSTRACT

A new method employing succession analysis and extremely high-frequency (EHF) irradiation is proposed for the selective isolation of actinomycetes from soil. Total actinomycetes were efficiently isolated from soil suspensions irradiated in the wavelength band 4.6-5.8 mm on the 14th and 45th days of succession initiated by soil wetting and from soil suspensions irradiated in the wavelength band 8-11.5 mm on the 7th day of succession. The rare actinomycete genera Actinomadura, Micromonospora, Nonomuraea, Microbispora, Amycolatopsis, Pseudonocardia, Saccharothrix, Streptosporangium, Actinosynnema, Nocardioides, and Saccharopolyspora were isolated by either of the two approaches (succession analysis and EHF irradiation); however, the range of isolated rare actinomycetes was considerably wider when the combination of the two approaches was used. For instance, actinomycetes of the rare genera Actinocorallia, Promicromonospora, Actinoplanes, and Kibdelosporangium were isolated only when EHF irradiation was employed at the early stages of succession.


Subject(s)
Actinobacteria/isolation & purification , Soil Microbiology , Actinobacteria/growth & development , Actinobacteria/radiation effects , Colony Count, Microbial , Radio Waves , Time Factors
2.
Mikrobiologiia ; 71(1): 119-22, 2002.
Article in Russian | MEDLINE | ID: mdl-11910800

ABSTRACT

A new method employing extremely high frequencies (EHFs) is proposed for the selective isolation of actinomycetes from soil. The pretreatment of soil suspensions with EHF wavelengths of 5.6 and 7.1 mm led to a nonselective isolation of actinomycetes. At the same time, the irradiation of soil suspensions within wavelength bands of 3.8-5.8 and 8-11.5 mm considerably augmented the total number of isolated actinomycetes and increased the fraction of the isolated rare genera by 2 and 7 times, respectively. The rare actinomycete genera were represented by Actinomadura, Microtetraspora, Nonomuraea, Micromonospora, Amycolatopsis, Pseudonocardia, Saccharotrix, and Streptosporangium.


Subject(s)
Actinomycetales/isolation & purification , Radio Waves , Soil Microbiology , Microbiological Techniques
3.
Crit Rev Biomed Eng ; 28(5-6): 40-51, 2000.
Article in English | MEDLINE | ID: mdl-11211984

ABSTRACT

The effect of microwaves on liquid water was investigated by using biosensors and physiochemical techniques, such as nuclear magnetic resonance (NMR) and spectrophotometry. The results obtained provided evidence for a nonthermal effect of microwaves on liquid water.


Subject(s)
Microwaves , Water/chemistry , Biophysical Phenomena , Biophysics , Biosensing Techniques , Chemical Phenomena , Chemistry, Physical , Chlorophyta/chemistry , Hydrogen Bonding , Magnetic Resonance Spectroscopy , Spectrophotometry
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