ABSTRACT
The aim of this paper is to quantify the adhered fibronectin (FN; by adsorption and/or grafting) and the exposure of its cell adhesive motifs (RGD and FNIII7-10) on poly(ethyl acrylate) (PEA) copolymers whose chemical composition has been designed to increase wettability and to introduce acid functional groups. FN was adsorbed to PEA, poly(ethyl acrylate-co-hydroxyethyl acrylate), poly(ethyl acrylate-co-acrylic acid), and poly(ethyl acrylate-co-methacrylic acid) copolymers, and covalently cross-linked to poly(ethyl acrylate-co-acrylic acid) and poly(ethyl acrylate-co-methacrylic acid) copolymers. Amount of adhered FN and exhibition of RGD and FNIII7- 10 fragments involved in cell adhesion were quantified with enzyme-linked immunosorbent assay tests. Even copolymers with a lower content of the hydrophilic component showed a decrease in water contact angle. In addition, FN was successfully fixed on all surfaces, especially on the hydrophobic surfaces. However, it was demonstrated that exposure of its cell adhesion sequences, which is the key factor in cell adhesion and proliferation, was higher for hydrophilic surfaces.
Subject(s)
Acrylic Resins/chemistry , Coated Materials, Biocompatible/chemistry , Fibronectins/chemistry , Acrylates/chemistry , Adsorption , Amino Acid Motifs , Cell Adhesion , Cells, Cultured , Humans , Materials Testing , Methacrylates/chemistry , Oligopeptides/chemistry , Surface Properties , WettabilityABSTRACT
The properties of the biomaterials used to constitute lenses are important factors choosing a lens for human implantation because these can influence in posterior clinical evolutions of patients. In this study, different characteristics of intraocular lenses such as chemical composition, surface roughness and lens design have been investigated in terms of their influence into a pathological environment. Eight commercial lenses were tested by optical profiling, Infrared spectra with Fourier transformation (FTIR), water-material contact angle and scanning electron microscope (SEM) to know their chemical composition and structural characteristics. These lenses were then exposed to infectious conditions in order to evaluate their responses to the bacterial environment.
