ABSTRACT
1. The objective of this study was to evaluate the prediction potential of a computer tomography (CT) data collection protocol for determining total body composition used for analysis of tibiotarsal bone quality features. 2. The CT image acquisition was performed on 54 healthy TETRA SL genotype laying hens at 90 weeks of age as well as in the 69th week of the egg production period in vivo and their tibiotarsal bones, ex vivo. 3. Breaking strengths and ash content of the tibiotarsal bones were estimated based on the calculated mineral density of skeletal and tibiotarsal bones by means of CT with an estimation accuracy R2 0.963 and 0.975, respectively. 4. In conclusion, the current investigation demonstrated that the acquisition protocol of CT for total-body composition analysis has a good potential for measuring the mineral status and breaking strength of the reference bone in laying hen.
Subject(s)
Bone Density , Chickens/physiology , Tarsus, Animal/physiology , Tibia/physiology , Animals , Body Composition , Female , Minerals/analysis , Reproduction , Tarsus, Animal/growth & development , Tibia/diagnostic imaging , Tomography, X-Ray Computed/veterinaryABSTRACT
The extensively investigated serine/threonine kinase, B-RAF, is a member of the RAS/RAF/MEK/ERK pathway. It plays important role in the regulation of cell growth, differentiation and survival. The mutation of B-RAF occurs frequently in melanomas and colon tumors; therefore, it is considered as an outstanding therapeutic target. In recent years a great number of B-RAF inhibitors have been reported and this number is expected to increase. The aim of our work was to compare the structures and binding mode of the published B-RAF inhibitors, and then to apply the correlations found for the explanation of our experimental results. In the first part of this paper we describe the main pharmacophore features of the co-crysallized B-RAF inhibitors published in the literature, focusing on the binding modes and common structural elements. In the second part we present and characterize our recently developed B-RAF inhibitor family by application of in silico methods and in vitro kinetic profiling. The inhibitory activity of these compounds was determined in biochemical kinase- and cell-based assays. The docking and assay results support our conclusion that the presented compound family belongs to the type I 1/2 subgroup, they inhibit B-RAF and B-RAF(V600E) mutant in a sub-micromolar range and most of them show selectivity towards B-RAF(V600E) mutant expressing cell lines with equal or even better IC50 values than sorafenib.
Subject(s)
Protein Kinase Inhibitors/chemistry , Protein Kinase Inhibitors/pharmacology , Proto-Oncogene Proteins B-raf/antagonists & inhibitors , Small Molecule Libraries/chemistry , Small Molecule Libraries/pharmacology , Animals , Cell Line, Tumor , Humans , Molecular Docking Simulation , Mutation , Protein Binding , Proto-Oncogene Proteins B-raf/genetics , Proto-Oncogene Proteins B-raf/metabolismABSTRACT
Magnetic resonance imaging (MRI) was tested for evaluation of the soft tissue structures of the equine digit in 16 limbs, derived from three adult warmblood horses and two newborn warmblood foals. The following measuring sequences were used in sagittal, transversal and coronal planes: spin echo, gradient echo, inversion recovery. The images were made with a 1.5 Tesla Siemens scanner in a CP-Helmholtz circular coil. To compare the visualization of the same tissue structures in adult and in newborn cases the limbs were imaged with the routinely used MRI sequences that are used for mature tissues. In newborn foals the bursa podotrochlearis could not be determined with the used sequences. For both the adult and newborn limbs the most visualization of the same tissue structures including the fluid spaces and growth plates were taken by the inversion recovery sequence. T2 sequence was very informative in adult cases but moderately in newborn foals.
Subject(s)
Horses/anatomy & histology , Magnetic Resonance Imaging/veterinary , Tarsal Bones/anatomy & histology , Animals , Animals, Newborn/anatomy & histology , Female , Forelimb/pathology , Hindlimb/pathology , Horse Diseases/pathology , Lameness, Animal/pathology , Male , Predictive Value of Tests , Tarsal Bones/growth & developmentABSTRACT
The cardiovascular capacity of turkeys is considerably affected by the selection procedure for meat production. To determine the body weight-related changes of some quantitative characteristics of the turkey heart function, serial measurements were carried out. BUT Big 6 turkeys (both sexes) were examined using whole-body magnetic resonance imaging (MRI) and spiral computer tomography (CT). Based on dynamic MRI examinations the following significant age- and sex-dependent quantitative differences were found. Means of the left ventricular stroke volumes were measured at the ages of 12 wk (males, 4.18 mL; females 2.89 mL), 16 wk (males, 6.56 mL; females, 4.14 mL), and 20 wk (males, 8.17 mL; females, 5.16 mL). Average cardiac output (CO) values were 0.76, 1.14, and 1.33 L/min for males and 0.58, 0.79,0.93 L/min for females. A statistically unproven age-dependent increasing tendency in the estimated relative CO value (skeletal muscle tissue volume measured by CT/CO unit) of male and female turkeys was found under sedentary conditions. These changes in the relative CO value and also the determined decrease of CO value related to body surface may, in theory, indicate a disadvantageous process that is more pronounced in males. The combined application of CT and MRI can be a powerful approach for studying the relationship between skeletal muscle development and heart capacity.
