A device to apply user-specified strains to biomaterials in culture.

An apparatus was developed to apply user-specified displacements to biomaterial samples in culture. The device allowed cyclic waveforms of bandwidth 0 Hz to 20 Hz to be applied under physiologic thermal (37.5 degrees C) and [CO2] (5%) conditions. For a 0 Hz to 20 Hz bandwidth signal similar in shape to a ventricular pressure waveform, the mean displacement error was 0.26% of the full-scale output. The maximum overshoot was 0.700%. Environmental system evaluation tests demonstrated a specimen cartridge temperature of 37.20 +/- 0.15 degrees C during cyclic loading and 37.23 +/- 0.21 degrees C during static conditions. [CO2] was 5.29 +/- 0.54% during cyclic loading and 5.25 +/- 0.61% during static conditions. Laminar flow applied at the loading rod entrances to the specimen cartridge ensured the sample remained sterile during testing. As a preliminary evaluation, polyurethane samples were seeded with fetal foreskin fibroblasts and subject to intermittent cyclic displacements. Results demonstrated enhanced cell proliferation and increased [PGE2] for samples subjected to 10% strain compared with unstrained controls. A next step will be to evaluate cell response sensitivity to strain magnitude, duration, direction, and frequency. The long-term intent is to establish mechanical loading configurations that induce acceptable or adaptation-inducing responses for use in implant design and tissue engineering applications.

The Bloch equations in high-gradient magnetic resonance force microscopy: theory and experiment.

We report theory and observations of paramagnetic resonance in a measured field gradient of 44,000 T per meter by the technique of magnetic resonance force microscopy (MRFM). Resonance was induced in a dilute solid solution of diphenylpicrylhydrazyl in polystyrene at 77 and 10 K by an amplitude-modulated microwave field. This modulated the force between resonant sample spins and a micrometer-scale SmCo magnetic tip on a force microscope cantilever. The force signals were typically of order 10 fN, and were detected above a thermal noise floor of 80 aN per root hertz at 10 K, equivalent to a magnetic moment noise of 200 micro(B) per root hertz of bandwidth. Resonance saturation was readily observed. Starting with the Bloch equations, we derived simple analytic expressions for the predicted cantilever signal amplitudes and T(1)-dependent phase lags, valid at low microwave power levels. For power levels below saturation, the data were in good agreement with the Bloch equation predictions, while above saturation the measured force increased more slowly with power than predicted. Several ESR mechanisms which might lead to non-Bloch dynamics in the MRFM environment are reviewed. Spin-relaxation mechanisms are also reviewed. A detailed description of the experimental apparatus is offered.

A bidirectional load applicator for the investigation of skin response to mechanical stress.

Instrumentation was developed to apply controlled biaxial (normal and shear) forces to the skin of a human or animal subject. The instrument mimicked any reference waveform within the constraints of a bandwidth of 15 Hz, a maximum force of 20 N, and displacement ranges of 15 mm for the normal direction and 18 mm for the shear direction. Two shaker motors, positioned with their axes parallel, were used with a low effective mass linkage and small-angle rotational joints to deliver the force. A digital feedback controller independently controlled the instantaneous normal and shear forces and recorded the resultant displacements. Evaluations on human and animal (pig) subjects demonstrated mean absolute errors between the applied and reference waveforms of less than 1.2% full-scale output for both the normal and shear directions. No degradation in performance was apparent over the course of a 1-h loading session. The instrument is to be used for the investigation of skin adaptation to mechanical stress, information that could be used to design new therapeutic methods to encourage skin load-tolerance.

Robotic assistance in orthopaedic surgery. A proof of principle using distal femoral arthroplasty.

The term "robot" refers to a precision mechanical device that is accurately controlled by a computer using intelligent software. The term "robotic assistance" refers to the use of such a device to aid a surgeon in the optimal conduct of a procedure, particularly one requiring specified geometrical relationships. The authors have been exploring the application of robotic assistance in situations in which accuracy and precision are required in orthopaedic surgery. The initial application concerned the planning, positioning, and orientation cuts and holes of the bone required for the femoral component of a total knee arthroplasty. A three-dimensional digitizing template allowed the surgeon to specify the desired position and orientation of the component's articular surfaces in relation to the distal femur. The robotic system used this spatial relationship, along with its knowledge of the geometry of the component selected by the surgeon, to plan the precise location of the required bone cuts and holes. Finally, the robotic assistant sequentially positioned saw and drill guides with respect to the distal femur so that the surgeon made these cuts and holes in the locations necessary for optimal component fit, position, and orientation. The robotic assistant functioned easily in the operating room environment; increased the accuracy; and decreased the time, equipment, and personnel required for the conduct of the geometrical part of this surgical procedure.

A geometric theory of the equilibrium mechanics of fibers in ligaments and tendons.

This paper presents a theoretical analysis of the equilibrium mechanics of bending and twisting fiber geometries in ligaments and tendons. The theory predicts that the bending of loaded fibers is necessarily accompanied by large transverse pressures and pressure gradients. The predicted pressures are especially large at the bone tunnel entries of ligament grafts, where they can equal or exceed the applied tensile loads. Experimental measurements of internal pressures confirm these predictions.

Ligament length relationships in the moving knee.

This article presents an investigation of potential ligament attachment sites for surgical reconstruction of the anterior and posterior cruciate ligaments as well as for the lateral extraarticular iliotibial band tenodesis. Our methodology was based on quantitative measurements of knee anatomy and motion in fresh cadavers, not on biomechanical modeling. Using computer search techniques, we located all the ligament insertion sites that were nearly isometric for motion of the intact knee.