Evaluación de la estabilidad de parámetros hematológicos en muestras pediátricas bajo diferentes condiciones de almacenamiento determinados en un analizador Sysmex XN / Stability of hematological parameters in pediatric samples under different storage conditions using the Sysmex XN analyser / Avaliação de estabilidade de parâmetros hematológicos em amostras pediátricas sob diferentes condições de armazenamento determinados em um analisador Sysmex XN

Resumen Conocer la estabilidad de las muestras biológicas es esencial para obtener resultados confiables en el laboratorio de análisis clínicos. La Norma IRAM-ISO 15189:2023 establece que cada laboratorio debe estandarizar las condiciones de almacenamiento de las muestras. El objetivo de este trabajo fue determinar el tiempo y la temperatura óptimos para conservar muestras sanguíneas para la determinación de parámetros hematológicos en un analizador Sysmex XN 1000. Se procesaron muestras de sangre entera conservadas hasta 48 horas a temperatura ambiente y hasta 72 horas en heladera. De acuerdo a los resultados, para la determinación del hemograma, las muestras almacenadas a temperatura ambiente deben procesarse dentro de las 6-8 horas posextracción; los parámetros limitantes son aquellos influenciados por cambios en el volumen eritrocitario. En las muestras conservadas en heladera el análisis puede retrasarse hasta 48 horas y el recuento de plaquetas es el parámetro menos estable.

Abstract Knowing sample stability is essential to obtain reliable results in the clinical laboratory. The IRAM-ISO 15189:2023 Standard establishes that each laboratory must standardise the storage conditions of biological samples. This study was conducted to investigate the stability of haematological parameters using a Sysmex XN 1000 in samples stored for up to 48-72 hours at different temperatures. According to these results, the hematologic analytes should be processed in this laboratory within 6-8 hours after extraction if they are stored at room temperature. The limiting parameters are those influenced by changes in the erythrocyte volume. If storage is in a refrigerator, the analysis can be delayed up to 48 hours, being platelet count the limiting parameter.

Resumo Conhecer a estabilidade das amostras biológicas é essencial para obter resultados confiáveis no laboratório de exames clínicas. A Norma IRAM-ISO 15189:2023 estabelece que cada laboratório deve padronizar as condições de armazenamento das amostras. O objetivo deste trabalho foi determinar o tempo e a temperatura ideais para preservar amostras de sangue para a determinação de parâmetros hematológicos em um analisador em um Sysmex XN 1000. Para isso, foram processadas amostras de sangue total armazenadas por até 48 horas em temperatura ambiente e por até 72 horas em geladeira. De acordo com os resultados obtidos, para a determinação do hemograma, as amostras armazenadas em temperatura ambiente devem ser processadas dentro de 6-8 horas após a extração, sendo os parâmetros limitantes aqueles influenciados por alterações no volume eritrocitário. Nas amostras armazenadas em geladeira, a análise pode demorar até 48 horas, sendo a contagem de plaquetas o parâmetro menos estável.

Effects of work-to-family enrichment on psychological distress and family satisfaction: A dyadic analysis in dual-earner parents.

Resources that individuals acquire at work can have significant effects on their own well-being and family satisfaction, and on those of their closest family relations. Following the conservation of resources theory, this study examined the effects of work-to-family enrichment (WFE) on psychological distress (depression, anxiety, and stress) and family satisfaction (SWFaL) in different-gender dual-earner parents. The sample consisted of 473 couples - mother and father - from Temuco, Chile, who answered the Work-Family Interface scale, the Depression, Anxiety and Stress Scale, and the Satisfaction with Family Scale. The analysis was conducted using the Actor-Partner Interdependence Model (APIM). Results showed that WFE was positively associated with SWFaL for both mothers and fathers (actor effects), whereas higher WFE was linked to lower psychological distress only in fathers. For mothers and fathers, psychological distress was negatively associated with their own (actor effects) and the other parent's SWFAL (partner effects). The mediating role of psychological distress was found only for fathers' WFE and their SWFaL. These results indicate that resources from work can increase family satisfaction for both fathers and mothers in dual-earner couples with adolescent children. However, in these couples, fathers acquire affective resources (positive mood) that reduce their own psychological distress, while mothers acquire skills (managing time at home more efficiently) that they invest directly in the family domain.

Influencia de los anticoagulantes EDTAK² y EDTAK³ en los resultados del hemograma / Influence of the EDTAK² and EDTAK³ anticoagulants on hematology testing results / Influência dos anticoagulantes EDTAK² e EDTAK³ nos resultados do hemograma

El International Council for Standardization in Haematology (ICSH) recomienda el uso de ácido etilendiaminotretaacético dipotásico (EDTAK2) como anticoagulante para el hemograma, mientras que el Clinical and Laboratory Standards Institute (CLSI) EDTAK2 o ácido etilendiaminotetraacético tripotásico (EDTAK3). El objetivo de este trabajo fue evaluar la influencia del tipo de sal de EDTA utilizado en la variación de los resultados de los parámetros hematológicos. Se recolectaron 24 muestras por venopunción, de cada una se cargó una alícuota en EDTAK3 y otra en EDTAK2. De los 23 parámetros evaluados en el autoanalizador Sysmex XN1000 (Roche Diagnostics), sólo los de la serie roja presentaron diferencias estadísticamente significativas en las muestras pareadas (p<0,05). El sesgo superó las especificaciones de calidad de Variabilidad Biológica Mínima para hematocrito (2,9%) y concentración de hemoglobina corpuscular media (1,7%), de Variabilidad Biológica Deseable para el volumen corpuscular medio (1,7%) y Variabilidad Biológica Óptima para hemoglobina (1,2%) y recuento de eritrocitos (1,2%). No se hallaron diferencias significativas en los parámetros de la serie blanca, ni en los plaquetarios y tampoco en los reticulocitarios. Para disminuir el error preanalítico, cada laboratorio debe estandarizar la extracción de los hemogramas empleando un único tipo de anticoagulante.

The International Council for Standardization in Haematology (ICSH) currently supports de use of ethylenediaminetetraacetic acid dipotassium (EDTAK2) anticoagulant for hematology testing, while the Clinical and Laboratory Standards Institute (CLSI) recommends EDTAK2 or ethylenediaminetetraacetic acid tripotassium (EDTAK3). The objective of this study was to evaluate the influence of the EDTA formulations in the results of hematologic parameters. A total of 24 samples were collected by venous puncture and two aliquots were loaded: one in EDTAK3 and another in EDTAK2. Of the 23 hematologic parameters tested with the use of Sysmex XN1000 (Roche diagnostics) hematologic analyser, only those in the red series showed statistically significant differences in the paired samples (p < 0.05). The bias exceeded the quality specifications of Minimum Biological Variability for hematocrit (2.9%) and mean corpuscular hemoglobin concentration (1.7%), Desirable Biological Variability for the mean corpuscular volume (1.7%) and Biological Variability Optimal for hemoglobin (1.2%) and erythrocyte count (1.2%). No significant differences were found in the parameters of white series, neither platelets, nor reticulocytes. To reduce the preanalytical error, each laboratory should standardize the extraction for complete blood cell count using a single type of anticoagulant.

A International Council for Standardization in Haematology (ICSH) recomenda a utilização do ácido etileno diamino treta-acético dipotássico (EDTAK2) como anticoagulante para hemograma, enquanto que a Clinical and Laboratory Standards Institute (CLSI) EDTAK2 ou ácido etileno diamino treta-acético tripotássico (EDTAK3). O objetivo deste trabalho foi avaliar a influência do tipo de sal de EDTA utilizado na variação dos resultados dos parâmetros hematológicos. Foram recolhidas 24 amostras por venopunção, de cada uma se carregou uma alíquota de EDTAK3 e outra de EDTAK2. Dos 23 parâmetros avaliados no autoanalisador Sysmex XN1000 (Roche Diagnostics), apenas aqueles da série vermelha apresentaram diferenças estatisticamente significativas nas amostras emparelhadas (p<0,05). O viés superou as especificações de qualidade da Variabilidade Biológica Mínima para o hematócrito (2,9%) e concentração de hemoglobina corpuscular média (1,7%), de Variabilidade Biológica Desejável para o volume corpuscular médio (1,7%) e Variabilidade Biológica Ótima para a hemoglobina (1,2%) e contagem de eritrócitos (1,2%). Não foram encontradas diferenças significativas nos parâmetros da série branca, nem nos plaquetários, nem nos reticulocitários. Para reduzir o erro pré-analítico, cada laboratório deve padronizar a extração dos hemogramas usando um único tipo de anticoagulante.

Conectividad cerebral en la regulación emocional: la solución de problemas emocionales / Brain connectivity in emotional regulation: the emotional solving problems

La solución de problemas emocionales es un mecanismo de regulación emocional que utiliza estrategias cognitivo-lingüísticas para reducir el impacto afectivo de una situación y lograr un objetivo propuesto. Es considerado un mecanismo saludable junto con la revaluación, a diferencia de la supresión y la rumiación. Estudios con técnicas de neuroimágenes han propuesto áreas cerebrales asociadas al funcionamiento de los diferentes mecanismos de regulación emocional. En este trabajo se propuso investigar los gradientes de conectividad cerebral de sustancia blanca asociados a la solución de problemas emocionales, con el objetivo de proponer una red de conectividad neuronal de este mecanismo de regulación emocional. Para evaluar la efectividad de la solución de problemas emocionales se utilizó una tarea conductual desarrollada recientemente basada en la recuperación semántica esforzada con estímulos de alto impacto afectivo. Participaron del estudio 32 mujeres jóvenes (M= 24,34; DS=5,24). Se obtuvieron coeficientes de correlación de Pearson entre las medidas del porcentaje de acierto de la tarea conductual y la anisotropía fraccional de los fascículos de sustancia blanca de interés. Los resultados proponen un red neuronal de solución de problemas emocionales en la que participarían principalmente el Fascículo Arqueado y Fronto Occipital Inferior del hemisferio izquierdo, cruciales en la implementación de estrategias de carácter léxico y semántico para la disminución del impacto afectivo (AU)

Emotional solving problems is an emotional regulation mechanism that implements cognitive-linguistics strategies to reduce the affective impact in a situation and to accomplish a proposed objective. Together with the reappraisal, it is considered a healthy mechanism, in difference with suppression and rumination. Researches that have used neuroimaging techniques have proposed cerebral areas associated to the functioning of the different emotional regulation mechanisms. The objective of this research was to study the brain connectivity gradients of white matter associated to emotional solving problems, to propose a brain connectivity network of this emotional regulation mechanism. The efficacy of emotional solving problems was measured with a behavioral task recently developed, based on semantic effortful retrieval with high affective impact stimulus. 32 young women (M= 24,34; DS=5,24) participated of the study. Correlations of Pearson were performed between accuracy measures of the behavioral task and fractional anisotropy of the white matter fascicles of interest. The results propose a brain connectivity network of emotional solving problems with the main participation of the Arcuate and Fronto Occipital Inferior fascicles of left hemisphere, both crucial in the implementation of lexical and semantic strategies to the reduction of affective impact (AU)

Oriented immobilisation of anti-pneumolysin Fab through a histidine tag for electrochemical immunosensors.

Orientation of reagents is a key step in the construction of immunosensors. When the immunoreagent is a recombinant protein, this can be achieved by employing hexahistidine tags. The orientation of recombinant histidine-tagged Fab fragments of monoclonal anti-pneumolysin antibodies on gold films is evaluated. Using histidine as a chelator of Ni or employing an anti-polyhistidine antibody for capturing the His6 residue is considered. Measurements are based in the signal of indigo, which comes from the hydrolysis of 3-indoxylphosphate by alkaline phosphatase (AP). The attachment of the enzyme occurs through the interaction of biotin with AP-labelled streptavidin or employing AP-conjugated immunoreagents. In the case of the interaction Ni-histidine, for the study of the self-assembling process a His-tagged and biotinylated protein (His6-GST-B) was employed. General conditions were studied and non-specific adsorption was avoided with the use of 1-hexanethiol. Improvements of the signal compared with the direct adsorption were only achieved by the use of histidine capturing antibodies. With an optimised ratio anti-polyhis:His6-Fab the signal increases approximately a 100%. Precision is adequate and the response is linear with the concentration of pneumolysin between 0.1 and 10 ng/mL.