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1.
Rev. iberoam. fertil. reprod. hum ; 33(1): 15-26, ene.-mar. 2016. tab, graf
Article in Spanish | IBECS | ID: ibc-149932

ABSTRACT

La infertilidad, es un problema que afecta a una gran cantidad de parejas. Una de sus causas es la disminución de la calidad seminal debido, por ejemplo, a tratamientos gonadotóxicos. La criopreservación seminal es la técnica que permite conservar y almacenar espermatozoides sin que pierdan su capacidad fecundante; siendo esta una herramienta fundamental en reproducción asistida. El objetivo de este trabajo ha sido optimizar la técnica de criopreservación. Para ello se llevó a cabo un estudio, sobre muestras de pacientes en estudio por problemas de fertilidad, en el que se compararon dos medios de criopreservación (SpermCryo™All-round y CryoSperm™) y la aplicación o no de un baño en nitrógeno líquido a las muestras (previo a su almacenamiento); así como el efecto del tiempo que transcurre desde la eyaculación hasta el procesado sobre la calidad de la muestra. Las posibles variaciones fueron estudiadas con un analizador automático, mediante la realización de test pre- y post-congelación para comprobarla movilidad espermática


Infertility is a problem that affects a lot of couples. One of its causes is a decreased semen quality due to, for example, gonadotoxic treatments. The cryopreservation of human semen is the technique that allows sperm preserving and storing without losing their fertilizing capacity; being a fundamental tool in assisted reproduction. The aim of this study was to optimize the cryopreservation technique. To this end, a study carried out on samples of patients under study by fertility problems, in which two cryoprotectant media (SpermCryo™ All-round and CryoSperm™) and the execution or non-execution of an immersion of the samples in liquid nitrogen (before storage) were compared; and the effect of the time between ejaculation and the processing on the quality of the sample. Variations were studied with an automatic analyzer by performing pre- and post-thaw sperm motility tests. The results show no difference between the two cryoprotectants media, but seems to have a tendency to obtain better postthaw mobility with either depending on sample characteristics. Moreover, the liquid nitrogen bath had no apparent effects on post-thaw results. However, we must highlight the importance of time in the processing of semen samples once liquefied, to avoid decreased sperm quality. To improve post-thaw outcomes the key lies in the necessity to adjust the freezing protocol to the sample characteristics and a correct implementation of the protocol cryopreservation (selection and addition of cryoprotectant media...); favoring the management of infertility and the success of assisted reproduction techniques


Subject(s)
Humans , Male , Adult , Middle Aged , Sperm Motility/physiology , Infertility, Male/epidemiology , Semen Analysis/methods , Cryoprotective Agents/analysis , Cryoprotective Agents/therapeutic use , Cryopreservation/methods , Cryopreservation , Sperm Immobilizing Agents/therapeutic use , Sperm Transport/physiology
2.
Rev. iberoam. fertil. reprod. hum ; 32(4): 35-44, oct.-dic. 2015. graf, tab
Article in Spanish | IBECS | ID: ibc-147130

ABSTRACT

La infertilidad, es un problema que a día de hoy afecta a una gran cantidad de parejas. Una de las posibles causas es la disminución a nivel mundial de la calidad seminal debido a factores como pueden ser la alimentación, el consumo de alcohol y tabaco, altos niveles de estrés o factores ambientales. Además, el aumento de la incidencia de enfermedades tumorales en pacientes menores de 35 años, pone de manifiesto la necesidad de preservar la fertilidad de estos pacientes mediante técnicas de criopreservación. Es importante, que ésta se realice antes de que reciban ningún tipo de tratamiento (quimioterapia y radioterapia). En este trabajo, se llevará a cabo un estudio comparativo entre las muestras de varones que acuden al Hospital Materno Infantil por problemas de fertilidad y aquellos pacientes oncológicos que antes de iniciar el tratamiento realizan una criopreservación de la muestra seminal analizando las posibles variaciones de estas últimas muestras, mediante un test de post-congelación para comprobar la viabilidad de las muestras criopreservadas. Los parámetros que se van a analizar son: edad del paciente, volumen de la muestra, concentración espermática, porcentaje de espermatozoides móviles e inmóviles, móviles progresivos y espermatozoides tipo 'a'


The infertility, is a problem affecting a large number of couples today. One of the possible causes is decreasing around the world of the seminal ability due to factors such as food, alcohol and tobacco consumption, high levels of stress or environmental factors. In addition, the increase in the incidence of tumour diseases in patients younger than 35 years, highlights the need to preserve the fertility of these patients through cryopreservation techniques. It is important that this be done before they receive any kind of treatment (chemotherapy and radiotherapy). In this work, we will take place a comparative study between the samples of male couples who come to the mother and child Hospital for fertility problems and cancer patients before treatment carried out a Cryopreservation of spermatozoa by analysing possible variations of these latest samples, using a test of post-congelacion to check the viability of samples criopreserved. The parameters will be analyzed are: the patient's age, volume of sample, sperm concentration, percentage of progressive mobile and stationary, mobile sperm and sperm type 'a'


Subject(s)
Humans , Male , Adult , Infertility, Male/genetics , Infertility, Male/pathology , Neoplasms/metabolism , Neoplasms/pathology , Semen Analysis/methods , Tumor Burden/genetics , Spermatozoa/pathology , Clinical Laboratory Techniques/methods , Infertility, Male/congenital , Infertility, Male/complications , Neoplasms/drug therapy , Neoplasms/radiotherapy , Semen Analysis/standards , Tumor Burden/physiology , Spermatozoa/metabolism , Clinical Laboratory Techniques/instrumentation
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