Estimating canopy nitrogen concentration of sugarcane crop using in situ spectroscopy.

Estimating nitrogen (N) concentration in situ is fundamental for managing the fertilization of the sugarcane crop. The purpose of this work was to develop estimation models that explain how N varies over time as a function of three spectral data transformations in two stages (plant cane and first ratoon) under variable rates of N application. A randomized complete-block experimental design was applied, with four levels of N fertilization: 0, 80, 160, and 240 kg N ha-1. Six sampling events were carried out during the rapid growth stage, where the canopy reflectance spectra with a hyperspectral sensor were measured, and tissue samples for N determination in plant cane and first ratoon were taken, from 60 days after emergence (DAE) and 60 days after harvest (DAH), respectively, until days 210 DAE and 210 DAH. To build the models, partial least squares regression analysis was used and was trained by three transformations of the spectral data: (i) average reflectance spectrum (R), (ii) multiple scatter correction and Savitzky-Golay filter MSC-SG) reflectance spectrum, and (iii) calculated vegetation indices (VIs).

Surface functionalizing of a lipid nanosystem to promote brain targeting: step-by-step design and physico-chemical characterization.

The use of lipid nanosystems as drug delivery to the central nervous system may be advantageous over the current strategies. The aim of this study was to develop and characterize functionalized liposomes for treatment of brain diseases. The covalent method of coupling IgG to liposomes via the derivatized lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[4-(p-maleimidophenyl)butyramide](MPB-PE) was investigated. Optimized coupling conditions are shown to result in the efficient conjugation of IgG to liposomes containing low concentrations of MPB-PE (3/1 SH:IgG). The qualitative analysis has shown that after the extrusion process, more homogeneous populations of vesicles have been obtained with a nanometric size suitable to be effective to further anchor the protein. Negative values of zeta potential demonstrate that they are stable systems. Lyophilization was used to maintain the stability of the formulation. These very interesting results encourage further investigations to formulate peptide- and protein-loaded immunoliposomes, making targeting of liposomes as an attractive approach for brain drug delivery.