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1.
Pflugers Arch ; 465(8): 1171-9, 2013 Aug.
Article in English | MEDLINE | ID: mdl-23475395

ABSTRACT

Extracellular ATP promotes an indirect contraction of airway smooth muscle via the secondary release of thromboxane A2 (TXA2) from airway epithelium. Our aim was to evaluate if common contractile agonists modify this response to ATP. Tracheas from sensitized guinea pigs were used to evaluate ATP-induced contractions before and after a transient contraction produced by histamine, carbachol, or serotonin. Epithelial mRNA for COX-1 and COX-2 was measured by RT-PCR and their expression assessed by immunohistochemistry. Compared with the initial response, ATP-induced contraction was potentiated by pretreatment with histamine, carbachol, or serotonin. Either suramin (antagonist of P2X and P2Y receptors) plus RB2 (antagonist of P2Y receptors) or indomethacin (inhibitor of COX-1 and COX-2) annulled the ATP-induced contraction, suggesting that it was mediated by P2Y receptor stimulation and TXA2 production. When COX-2 was inhibited by SC-58125 or thromboxane receptors were antagonized by SQ-29548, just the potentiation was abolished, leaving the basal response intact. Airway epithelial cells showed increased COX-2 mRNA after stimulation with histamine or carbachol, but not serotonin, while COX-1 mRNA was unaffected. Immunochemistry corroborated this upregulation of COX-2. In conclusion, we showed for the first time that histamine and carbachol cause hyperresponsiveness to ATP by upregulating COX-2 in airway epithelium, which likely increases TXA2 production. Serotonin-mediated hyperresponsiveness seems to be independent of COX-2 upregulation, but nonetheless is TXA2 dependent. Because acetylcholine, histamine, and serotonin can be present during asthmatic exacerbations, their potential interactions with ATP might be relevant in its pathophysiology.


Subject(s)
Adenosine Triphosphate/metabolism , Carbachol/pharmacology , Cyclooxygenase 2/metabolism , Histamine/pharmacology , Serotonin/pharmacology , Trachea/drug effects , Animals , Cyclooxygenase 1/genetics , Cyclooxygenase 1/metabolism , Cyclooxygenase 2/genetics , Cyclooxygenase 2 Inhibitors/pharmacology , Cyclooxygenase Inhibitors/pharmacology , Epithelial Cells/drug effects , Epithelial Cells/metabolism , Guinea Pigs , Male , Muscle Contraction/drug effects , Muscle Contraction/genetics , Muscle, Smooth/drug effects , Muscle, Smooth/metabolism , Purinergic P2X Receptor Antagonists/pharmacology , Purinergic P2Y Receptor Antagonists/pharmacology , RNA, Messenger/genetics , Receptors, Purinergic P2X/genetics , Receptors, Purinergic P2X/metabolism , Receptors, Purinergic P2Y/genetics , Receptors, Purinergic P2Y/metabolism , Respiratory Mucosa/drug effects , Respiratory Mucosa/metabolism , Thromboxane A2/genetics , Thromboxane A2/metabolism , Trachea/metabolism , Up-Regulation/drug effects , Up-Regulation/genetics
2.
Pflugers Arch ; 462(4): 573-85, 2011 Oct.
Article in English | MEDLINE | ID: mdl-21800025

ABSTRACT

In airway smooth muscle (ASM), adenosine 5'-triphosphate (ATP) induces a relaxation associated with prostaglandin production. We explored the role of K(+) currents (I (K)) in this relaxation. ATP relaxed the ASM, and this effect was abolished by indomethacin. Removal of airway epithelium slightly diminished the ATP-induced relaxation at lower concentration without modifying the responses to ATP at higher concentrations. ATPγS and UTP induced a concentration-dependent relaxation similar to ATP; α,ß-methylene-ATP was inactive from 1 to 100 µM. Suramin or reactive blue 2 (RB2), P2Y receptor antagonists, did not modify the relaxation, but their combination significantly reduced this effect of ATP. The relaxation was also inhibited by N-ethylmaleimide (NEM; which uncouples G proteins). In myocytes, the ATP-induced I (K) increment was not modified by suramin or RB2 but the combination of both drugs abolished it. This increment in the I (K) was also completely nullified by NEM and SQ 22,536. 4-Amynopyridine or iberiotoxin diminished the ATP-induced I (K) increment, and the combination of both substances diminished ATP-induced relaxation. The presence of P2Y(2) and P2Y(4) receptors in smooth muscle was corroborated by Western blot and confocal images. In conclusion, ATP: (1) produces relaxation by inducing the production of bronchodilator prostaglandins in airway smooth muscle, most likely by acting on P2Y(4) and P2Y(2) receptors; (2) induces I (K) increment through activation of the delayed rectifier K(+) channels and the high-conductance Ca(2+)-dependent K(+) channels, therefore both channels are implicated in the ATP-induced relaxation; and (3) this I (K) increment is mediated by prostaglandin production which in turns increase cAMP signaling pathway.


Subject(s)
Muscle, Smooth/physiology , Potassium Channels/physiology , Receptors, Purinergic P2Y/physiology , 4-Aminopyridine/pharmacology , Adenine/analogs & derivatives , Adenine/pharmacology , Adenosine Triphosphate , Animals , Ethylmaleimide/pharmacology , Guinea Pigs , Male , Muscle Relaxation/drug effects , Peptides/pharmacology , Prostaglandins , Suramin/pharmacology , Trachea/physiology , Triazines/pharmacology
3.
Synapse ; 61(9): 732-40, 2007 Sep.
Article in English | MEDLINE | ID: mdl-17568409

ABSTRACT

We recorded whole-cell ion currents induced by gamma-aminobutyric acid (I(GABA)) and serotonin (I(5-HT)) to investigate and characterize putative interactions between GABA(A) and 5-HT(3) receptors in myenteric neurons from the guinea pig small intestine. I(GABA) and I(5-HT) were inhibited by bicuculline and ondansetron, respectively. Currents induced by the simultaneous application of both, GABA and 5-HT (I(GABA+5-HT)) were significantly lower than the sum of I(GABA) and I(5-HT), indicating the existence of a current occlusion. Such an occlusion was observed when GABA(A) and 5-HT(3) receptors are virtually saturated. Kinetics, and pharmacological properties of I(GABA+5-HT) indicate that they are mediated by activation of both, GABA(A) and 5-HT(3) channels. GABA did not alter I(5-HT) in neurons without GABA(A) channels, in the presence of bicuculline (a GABA(A) receptor antagonist) or at the reversal potential for I(GABA). Similarly, 5-HT did not modify I(GABA) in neurons in which 5-HT(3) channels were absent, after inhibiting 5-HT(3) channels with ondansetron (a 5-HT(3) receptor antagonist) or at the reversal potential for I(5-HT). Current occlusion was observed as soon as GABA(A) and 5-HT(3) channels were being activated, in the absence of Ca(2+), at low temperature (11 degrees C), and after adding staurosporine (a protein kinase inhibitor) to the pipette solution. Our proposal is that GABA(A) and 5-HT(3) channels are organized in clusters and within these, both channels can cross-inhibit each other, likely by allosteric interactions between these proteins.


Subject(s)
Myenteric Plexus/cytology , Neurons/physiology , Receptors, GABA-A/physiology , Receptors, Serotonin, 5-HT3/physiology , Animals , Cells, Cultured , Drug Interactions , Female , GABA Agents/pharmacology , Guinea Pigs , Male , Membrane Potentials/drug effects , Membrane Potentials/physiology , Membrane Potentials/radiation effects , Patch-Clamp Techniques/methods , Serotonin/pharmacology , Serotonin Agents/pharmacology , gamma-Aminobutyric Acid/pharmacology
4.
Eur J Neurosci ; 23(12): 3259-68, 2006 Jun.
Article in English | MEDLINE | ID: mdl-16820016

ABSTRACT

Inhibitory interactions between GABA(A)[induced by gamma-aminobutyric acid (GABA)] and P2X [activated by adenosine 5'-triphosphate (ATP)] receptors of myenteric neurones from the guinea pig small intestine were characterized using whole-cell recordings. Currents induced by GABA (I(GABA)) or ATP (I(ATP)) were inhibited by picrotoxin or pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid, respectively. Currents induced by GABA + ATP (I(GABA+ATP)) were only as large as the current induced by the most effective transmitter, revealing current occlusion. This occlusion requires maximal activation of at least one of these receptors. Sequential applications of neurotransmitters, and kinetic and pharmacological properties of I(GABA+ATP) indicate that they are carried through both GABA(A) and P2X channels. ATP did not affect I(GABA) in neurones: (i) in which P2X channels were not present; (ii) after inhibiting P2X channels with Ca2+ (iii) in the presence of pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid, a P2X receptor antagonist; (iv) after P2X receptor desensitization or (v) at I(ATP) reversal potential. Similarly, GABA did not affect P2X-mediated currents in neurones: (i) in which GABA(A) channels were not present; (ii) in the presence of picrotoxin, a GABA(A) channel blocker; (iii) after GABA(A) receptor desensitization or (iv) at the I(GABA) reversal potential. Current occlusion occurred as fast as current activation and it was still present in the absence of Ca2+, at 11 degrees C, after adding to the pipette solution a cocktail of protein kinase inhibitors (staurosporine + genistein + K-252a), after substituting the GTP in the pipette with GDP-beta-S and after treating the cells with N-ethylmaleimide. Taken together, all of these results are consistent with a model of cross-inhibition between GABA(A) and P2X.


Subject(s)
Myenteric Plexus/cytology , Neurons/metabolism , Receptors, GABA-A/metabolism , Receptors, Purinergic P2/metabolism , Adenosine Triphosphate/metabolism , Animals , Calcium/metabolism , Female , GABA Antagonists/metabolism , GTP-Binding Proteins/metabolism , Guinea Pigs , Male , Neurons/cytology , Patch-Clamp Techniques , Picrotoxin/metabolism , Pyridoxal Phosphate/analogs & derivatives , Pyridoxal Phosphate/metabolism , Receptors, Purinergic P2X , gamma-Aminobutyric Acid/metabolism
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