Subject(s)
Hypersensitivity , Iodine , Nuclear Medicine , Contrast Media , Humans , Radionuclide ImagingABSTRACT
Thin-layer radiochromatography is used for carrying out radiochemical purity control of gamma-emitter radiopharmaceuticals by using thin-layer radiochromatography scanners. It has been developed and implemented a simple method for validating the proper functioning of these instruments. This method helps to ensure accurate results in the radiochemical purity controls of gamma-emitter radiopharmaceuticals.
Subject(s)
Chromatography, Thin Layer/methods , Chromatography, Thin Layer/standards , Drug Contamination/prevention & control , Radiopharmaceuticals/analysis , Radiopharmaceuticals/chemistry , Quality Control , Radiochemistry , Reproducibility of Results , Sensitivity and SpecificityABSTRACT
This work describes the development of a software application for reporting patient radiation dosimetry following radiopharmaceutical administration. The resulting report may be included within the patient's medical records. The application was developed in the Visual Basic programming language. The dosimetric calculations are based on the values given by the International Commission on Radiological Protection (ICRP). The software is available in both Spanish and English and can be downloaded at no cost from www.radiopharmacy.net.
Subject(s)
Documentation/methods , Electronic Health Records/organization & administration , Information Storage and Retrieval/methods , Radiometry/classification , Radiopharmaceuticals/classification , Software , Internet , User-Computer InterfaceABSTRACT
OBJECTIVE: To test a new system for the biotyping of Streptococcus mutans, based on the measurement of enzyme activity, and to investigate the relationship between biotype and in vitro susceptibility to seven clinically useful antibiotics. METHODS: In total, 160 oral isolates of S. mutans were classified into different biotypes with the APIZYM test for enzyme activity, excluding results that were positive or negative in >80% of the strains. The susceptibility of all 160 strains to amoxycillin, cefazolin, erythromycin, clindamycin, vancomycin, teicoplanin and imipenem was tested by dilution in a solid medium. Statistical analysis of susceptibility (mean minimum inhibitory concentrations (MICs)) was based on chi-squared tests. RESULTS: Eight different biotypes (1-8) were identified on the basis of three kinds of enzyme activity: valine aryl amidase, acid phosphatase and alpha-galactosidase. Biotype 5 was found to be the most common. The mean MIC values showed strains belonging to biotype 4 to be the most susceptible to amoxycillin, cefazolin and erythromycin, whereas biotype 1 was the least susceptible to teicoplanin. CONCLUSIONS: The proposed biotyping method, which is relatively fast and simple to perform, provided reproducible results, and may contribute to clinically effective treatment of S. mutans infections.
