The Immune Response of OAS1, IRF9, and IFI6 Genes in the Pathogenesis of COVID-19.

COVID-19 is characterized by a wide range of clinical manifestations, where aging, underlying diseases, and genetic background are related to worse outcomes. In the present study, the differential expression of seven genes related to immunity, IRF9, CCL5, IFI6, TGFB1, IL1B, OAS1, and TFRC, was analyzed in individuals with COVID-19 diagnoses of different disease severities. Two-step RT-qPCR was performed to determine the relative gene expression in whole-blood samples from 160 individuals. The expression of OAS1 (p < 0.05) and IFI6 (p < 0.05) was higher in moderate hospitalized cases than in severe ones. Increased gene expression of OAS1 (OR = 0.64, CI = 0.52-0.79; p = 0.001), IRF9 (OR = 0.581, CI = 0.43-0.79; p = 0.001), and IFI6 (OR = 0.544, CI = 0.39-0.69; p < 0.001) was associated with a lower risk of requiring IMV. Moreover, TGFB1 (OR = 0.646, CI = 0.50-0.83; p = 0.001), CCL5 (OR = 0.57, CI = 0.39-0.83; p = 0.003), IRF9 (OR = 0.80, CI = 0.653-0.979; p = 0.03), and IFI6 (OR = 0.827, CI = 0.69-0.991; p = 0.039) expression was associated with patient survival. In conclusion, the relevance of OAS1, IRF9, and IFI6 in controlling the viral infection was confirmed.

Differential expression of antiviral and immune-related genes in individuals with COVID-19 asymptomatic or with mild symptoms.

COVID-19 is characterized by a wide range of symptoms where the genetic background plays a key role in SARS-CoV-2 infection. In this study, the relative expression of IRF9, CCL5, IFI6, TGFB1, IL1B, OAS1, and TFRC genes (related to immunity and antiviral activity) was analyzed in upper airway samples from 127 individuals (97 COVID-19 positive and 30 controls) by using a two-step RT-PCR. All genes excepting IL1B (p=0.878) showed a significantly higher expression (p<0.005) in COVID-19 cases than in the samples from the control group suggesting that in asymptomatic-mild cases antiviral and immune system cells recruitment gene expression is being promoted. Moreover, IFI6 (p=0.002) and OAS1 (p=0.044) were upregulated in cases with high viral loads, which could be related to protection against severe forms of this viral infection. In addition, a higher frequency (68.7%) of individuals infected with the Omicron variant presented higher viral load values of infection when compared to individuals infected with other variants (p<0.001). Furthermore, an increased expression of IRF9 (p<0.001), IFI6 (p<0.001), OAS1 (p=0.011), CCL5, (p=0.003) and TGFB1 (p<0.001) genes was observed in individuals infected with SARS-CoV-2 wildtype virus, which might be due to immune response evasion of the viral variants and/or vaccination. The obtained results indicate a protective role of IFI6, OAS1 and IRF9 in asymptomatic -mild cases of SARS-CoV-2 infection while the role of TGFB1 and CCL5 in the pathogenesis of the disease is still unclear. The importance of studying the dysregulation of immune genes in relation to the infective variant is stand out in this study.

Expression of angiogenic and lymphangiogenic genes in primary cutaneous melanoma: relationship with angiolymphatic invasion and disease-free survival.

Melanoma is one of the most common cancers in the world. The main routes of tumor progression are related to angiogenesis and lymphangiogenesis. These routes can occur by local invasion, which is called angiolymphatic invasion (ALI). In this study, we assess gene expression of relevant biomarkers of angiogenesis and lymphangiogenesis in 80 FFPE melanoma samples to determine a molecular profile that correlates with ALI, tumor progression, and disease-free survival. The results were enhanced by a posttranscriptional analysis by an immunofluorescence assay. Three SNPs in the VEGFR-2 gene were genotyped in 237 malignant melanoma (MM) blood DNA samples by qPCR. A significant correlation was found for LYVE -1 and ALI, qualitative ( P  = 0.017) and quantitative ( P  = 0.005). An increased expression of protein LIVE-1 in ALI samples supported these results ( P  = 0.032). VEGFR2 was lower in patients who showed disease progression ( P  = 0.005) and protein VEGFR2 posttranscriptional expression decreased ( P  = 0.016). DFS curves showed differences ( P  = 0.023) for VEGFR2 expression detected versus the absence of VEGFR2 expression. No significant influence on DFS was detected for the remaining analyzed genes. Cox regression analysis suggested that VEGFR2 expression has a protective role (HR = 0.728; 95% CI = 0.552-0.962; P  = 0.025) on disease progression. No significant association was found between any of the studied SNPs of VEGFR2 and DFS or progression rate. Our main results suggest that LYVE-1 gene expression is closely related to ALI; the relationship with the development of metastases in MM deserves further studies. Low expression of VEGFR2 was associated with disease progression and the expression of VEGFR2 correlates with an increased DFS.

Fungal Pathogens Associated with Aerial Symptoms of Avocado (Persea americana Mill.) in Tenerife (Canary Islands, Spain) Focused on Species of the Family Botryosphaeriaceae.

Fungi of the family Botryosphaeriaceae are considered responsible for various symptoms in avocado such as dieback, external necrosis of branches and inflorescences, cankers on branches and trunks, or stem-end rot of fruits. In recent years, these problems are becoming more frequent in avocado orchards in the Canary Islands (Spain). This work includes the characterization of fungal species involved in these diseases, which were isolated from avocado crops in Tenerife Island between 2018 and 2022. A total of 158 vegetal samples were collected, from which 297 fungal isolates were culture-isolated. Fifty-two of them were selected according to their morphological features as representative isolates of Botryosphaeriaceae, and their molecular characterization was carried out, sequencing the ITS1-2 region as well as the ß-tubulin and the elongation factor 1-alpha genes. Five species of Botryosphaeriaceae were isolated, including Neofusicoccum australe, N. cryptoaustrale/stellenboschiana, N. luteum, N. parvum, and Lasiodiplodia brasiliensis. This is the first time that L. brasiliensis has been associated with avocado dieback and that N. cryptoaustrale/stellenboschiana has been cited in avocado causing symptoms of dieback and stem-end rot. However, it was not possible to assign our isolates unequivocally to N. cryptoaustrale or N. stellenboschiana even additionally using the rpb2 marker for their molecular characterization. Botryosphaeriaceae family seem to be involved in avocado dieback, in the premature fall of fruits during their development in the field and in post-harvest damage in Tenerife, but further studies are needed to clarify the fungal pathogens associated with symptoms in relation to phenological plant growth stages or less frequently observed.

VEGFC Gene Expression Is Associated with Tumor Progression and Disease-Free Survival in Cutaneous Squamous Cell Carcinoma.

Cutaneous squamous cell carcinoma (CSCC) is one of the most common cancers in the skin. CSCC belongs to the non-melanoma skin cancers, and its incidence is increasing every year around the world. The principal routes of tumor progression are related to angiogenesis and lymphangiogenesis. In this study, we assess the gene expression of the relevant biomarkers of both routes in 49 formalin-fixed paraffin-embedded (FFPE) CSCC samples in an attempt to determine a molecular profile that correlates with the tumor progression and disease-free survival (DFS). The results were enhanced by a posttranscriptional analysis using an immunofluorescence assay. Overexpression of the vascular endothelial growth factor C (VEGFC) gene was found in patients with tumor progression (p = 0.022) and in patients with perineural invasion (p = 0.030). An increased expression of protein VEGFC in samples with tumor progression supported these results (p = 0.050). In addition, DFS curves showed differences (p = 0.027) for tumors with absent-low VEGFC expression versus those with high levels of VEGFC expression. No significant influence on DFS was detected for the remaining analyzed genes. VEGFC expression was found to be a risk factor in the disease progression (HR = 2.675; 95% CI: 1.089-6.570; p = 0.032). Our main results suggest that VEGFC gene expression is closely related to tumor progression, DFS, and the presence of perineural invasion.

Ozone Eliminates SARS-CoV-2 from Difficult-to-Clean Office Supplies and Clinical Equipment.

(1) Background: Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) continues to cause profound health, economic, and social problems worldwide. The management and disinfection of materials used daily in health centers and common working environments have prompted concerns about the control of coronavirus disease 2019 (COVID-19) infection risk. Ozone is a powerful oxidizing agent that has been widely used in disinfection processes for decades. The aim of this study was to assess the optimal conditions of ozone treatment for the elimination of heat-inactivated SARS-CoV-2 from office supplies (personal computer monitors, keyboards, and computer mice) and clinical equipment (continuous positive airway pressure tubes and personal protective equipment) that are difficult to clean. (2) Methods: The office supplies and clinical equipment were contaminated in an area of 1 cm2 with 1 × 104 viral units of a heat-inactivated SARS-CoV-2 strain, then treated with ozone using two different ozone devices: a specifically designed ozonation chamber (for low-medium ozone concentrations over large volumes) and a clinical ozone generator (for high ozone concentrations over small volumes). SARS-CoV-2 gene detection was carried out using quantitative real-time polymerase chain reaction (RT-qPCR). (3) Results: At high ozone concentrations over small surfaces, the ozone eliminated SARS-CoV-2 RNA in short time periods-i.e., 10 min (at 4000 ppm) or less. The optimum ozone concentration over large volumes was 90 ppm for 120 min in ambient conditions (24 °C and 60-75% relative humidity). (4) Conclusions: This study showed that the appropriate ozone concentration and exposure time eliminated heat-inactivated SARS-CoV-2 RNA from the surfaces of different widely used clinical and office supplies, decreasing their risk of transmission, and improving their reutilization. Ozone may provide an additional tool to control the spread of the COVID-19 pandemic.

Ozone treatment effectively eliminates SARS-CoV-2 from infected face masks.

The current COVID-19 pandemic is causing profound health, economic, and social problems worldwide. The global shortage of medical and personal protective equipment (PPE) in specialized centers during the outbreak demonstrated the need for efficient methods to disinfect and recycle them in times of emergency. We have previously described that high ozone concentrations destroyed viral RNA in an inactivated SARS-CoV-2 strain within a few minutes. However, the efficient ozone dosages for active SARS-CoV-2 are still unknown. The present study aimed to evaluate the systematic effects of ozone exposure on face masks from hospitalized patients infected with SARS-CoV-2. Face masks from COVID-19 patients were collected and treated with a clinical ozone generator at high ozone concentrations in small volumes for short periods. The study focused on SARS-CoV-2 gene detection (assessed by real-time quantitative polymerase chain reaction (RT-qPCR)) and on the virus inactivation by in vitro studies. We assessed the effects of different high ozone concentrations and exposure times on decontamination efficiency. We showed that high ozone concentrations (10,000, 2,000, and 4,000 ppm) and short exposure times (10, 10, and 2 minutes, respectively), inactivated both the original strain and the B.1.1.7 strain of SARS-CoV-2 from 24 contaminated face masks from COVID-19 patients. The validation results showed that the best condition for SARS-CoV-2 inactivation was a treatment of 4,000 ppm of ozone for 2 minutes. Further studies are in progress to advance the potential applications of these findings.

Gene Expression Studies in Formalin-Fixed Paraffin-Embedded Samples of Cutaneous Cancer: The Need for Reference Genes.

Formalin-fixed paraffin-embedded (FFPE) tumour samples may provide crucial data regarding biomarkers for neoplasm progression. Analysis of gene expression is frequently used for this purpose. Therefore, mRNA expression needs to be normalized through comparison to reference genes. In this study, we establish which of the usually reported reference genes is the most reliable one in cutaneous malignant melanoma (MM) and cutaneous squamous cell carcinoma (CSCC). ACTB, TFRC, HPRT1 and TBP expression was quantified in 123 FFPE samples (74 MM and 49 CSCC biopsies) using qPCR. Expression stability was analysed by NormFinder and Bestkeeper softwares, and the direct comparison method between means and SD. The in-silico analysis with BestKeeper indicated that HPRT1 was more stable than ACTB and TFRC in MM (1.85 vs. 2.15) and CSCC tissues (2.09 vs. 2.33). The best option to NormFinder was ACTB gene (0.56) in MM and TFRC (0.26) in CSCC. The direct comparison method showed lower SD means of ACTB expression in MM (1.17) and TFRC expression in CSCC samples (1.00). When analysing the combination of two reference genes for improving stability, NormFinder indicated HPRT1 and ACTB to be the best for MM samples, and HPRT1 and TFRC genes for CSCC. In conclusion, HPRT1 and ACTB genes in combination are the most appropriate choice for normalization in gene expression studies in MM FFPE tissue, while the combination of HPRT1 and TFRC genes are the best option in analysing CSCC FFPE samples. These may be used consistently in forthcoming studies on gene expression in both tumours.

Persistence of SARS-CoV-2 infection on personal protective equipment (PPE).

BACKGROUND: SARS-CoV-2 stability and infection persistence has been studied on different surfaces, but scarce data exist related to personal protective equipment (PPE), moreover using realist viral loads for infection. Due to the importance for adequate PPE management to avoid risk of virus infection, RNA stability was evaluated on PPE. METHODS: Persistence of SARS-CoV-2 infection and detection of genomic RNA in PPE (gowns and face masks) were determined by in-vitro assays and RT-qPCR, respectively. Samples were infected with a clinical sample positive for SARS-CoV-2 (Clin-Inf), and with a heat-inactivated SARS-CoV-2 strain sample (Str-Inf) as a control. RESULTS: PPE samples infected with Clin-Inf were positive for the 3 viral genes on gowns up to 5 days post-infection, whereas these overall genes were detected up to 30 days in the case of face masks. However, gowns and FFP2 masks samples contaminated with Clin-Inf showed a cytopathic effect over VERO cells up to 5-7 days post-infection. CONCLUSIONS: SARS-CoV-2 RNA was detected on different PPE materials for 5 to 30 days, but PPE contaminated with the virus was infectious up to 5-7 days. These findings demonstrate the need to improve PPE management and to formulate strategies to introduce viricidal compounds in PPE fabrics.

Effects of Ozone Treatment on Personal Protective Equipment Contaminated with SARS-CoV-2.

BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is causing profound health, economic, and social problems worldwide. Management of personal protective equipment (PPE) and its potential limited availability have created concerns about the increased risks for healthcare professionals at hospitals and nursing homes. Ozone is a powerful oxidant agent. The objectives of this study were to examine the effects of ozone treatment on PPE contaminated with SARS-CoV-2, and to explore whether relative humidity could modify those effects. METHODS: PPE contaminated by heat-inactivated SARS-CoV-2 were treated with different ozone concentrations, exposure times, and relative humidity conditions. SARS-CoV-2 gene amplification was assessed by real-time polymerase chain reaction. RESULTS: There was no amplification of SARS-CoV-2 in PPE after the following ozone exposures: 30 s at 10,000 ppm (20 g/m3), 5 min at 4000 ppm, and 10 min at 2000 ppm. At lower ozone concentrations, 4-12 ppm (0.008-0.024 g/m3), the effects were highly dependent on the relative humidity conditions. CONCLUSIONS: Oxidative stress induced by ozone exposure eliminated heat-inactivated SARS-CoV-2 in different PPE components under appropriate exposure times, ozone concentrations, and relative humidity conditions. These findings could have implications in decreasing the risk of contamination associated with personal protective equipment management and in increasing its availability. Further research in the original SARS-CoV-2 strain is guaranteed.

Teacher as Social Context (TASC) Questionnaire in the Spanish Setting: Teacher Version / El cuestionario Teacher as Social Context (TASC) en España: versión para el profesorado

This study pioneered the construction or adaptation of a Spanish version of the Teacher as Social Context (TASC) Questionnaire. The current study included a sample of 410 secondary education teachers in Spain. A confirmatory factor analysis was performed using the maximum likelihood estimation; the factorial invariance of the TASC-Spanish teacher measure across genders was analyzed using a multi-group confirmatory factor analysis. Finally, correlational analyses between the three TASC scales (involvement, structure, and autonomy support) and behavioral and emotional engagement were run. The results of this research confirmed the structure of the original instrument maintaining the three basic domains. The measurement model also proved to be invariant across gender. Besides, this study supports the assumption that teachers' perceptions of their own activity to fulfil students' basic psychological needs show a relationship with students’ emotional and behavioural engagement, being teachers' involvement the most determinant domain

Este estudio ha sido pionero en la construcción o adaptación de una versión en español del cuestionario Teacher as Social Context (TASC). El estudio actual incluyó una muestra de 410 profesores de educación secundaria en España. Se realizó un análisis factorial confirmatorio utilizando la estimación de máxima verosimilitud; la invarianza factorial de la versión española del cuestionario TASC entre géneros se analizó utilizando un análisis factorial confirmatorio multigrupo. Por último, se realizaron análisis correlacionales entre las tres escalas TASC (participación, estructura y apoyo a la autonomía) y el compromiso conductual y emocional. Los resultados de esta investigación confirmaron la estructura del instrumento original manteniendo las tres dimensiones básicas. El modelo de medición también demostró ser invariable en función del género. Además, este estudio sustenta la suposición de que la percepción de los profesores sobre su propia actividad en relación con la satisfacción de las necesidades psicológicas básicas de los estudiantes muestra una relación con el compromiso emocional y conductual de estos, siendo la implicación de los profesores la dimensión más determinante

Validation of the Dependency-Oriented and Achievement-Oriented Psychological Control Scale (DAPCS) in a Spanish-speaking late adolescent sample / Validación de la Escala de Control Psicológico Orientado a la Dependencia y al Logro (DAPCS) en una Muestra de adolescentes tardíos que hablan español

Parental psychological control refers to those parental behaviors that interfere with the child's thoughts and feelings and are characterized by excessive use of manipulative techniques, such as inducing guilt or shame and love-withdrawal. This study was aimed at validating the Spanish version of the Dependency-oriented and Achievement-oriented Psychological Control Scale (DAPCS), a new measure designed to assess an adolescent's perception of psychological control. Another objective was to determine the influence of perceived parental psychological control on adolescents' self-concept. We evaluated the factor structure, reliability, and convergent validity in a sample of Spanish late adolescents (N = 310, mean age = 19.75). Exploratory and confirmatory factor analyses showed that the DAPCS factor structure is best described by a 2-factor solution, for both paternal and maternal ratings, indicating the distinction between dependency-oriented and achievement-oriented psychological control. High indices of internal consistency indicated that DAPCS produced reliable scores. Moreover, convergent validity was confirmed by consistent associations between the DAPCS and other measures of psychological control and other parenting dimensions. Hierarchical regression also showed that DAPCS predicted late adolescents' self-concept. The results of this study indicate that the Spanish version of the DAPCS is a useful instrument for studying both types of psychological control

El control psicológico parental se refiere a los comportamientos de los padres que interfieren con los Pensamientos y sentimientos del niño y se caracterizan por el uso excesivo de técnicas de manipulación, Como la inducción de culpa o vergüenza y la retirada del afecto. Este estudio tuvo como objetivo validar. La versión en español de la Dependency-oriented and Achievement - oriented Psychological Control Scale (DAPCS) (Escala de control psicológico orientado a la dependencia y control psicológico orientado Al logro), una nueva medida diseñada para evaluar la percepción del control psicológico de un adolescente. Otro objetivo fue determinar la influencia del control psicológico parental percibido sobre el Autoconcepto de los adolescentes. Evaluamos la estructura factorial, la fiabilidad y la validez convergente en una muestra de adolescentes tardíos españoles (N=310, edad media = 19.75). Los análisis de factores Exploratorios y confirmatorios mostraron que la estructura factorial de la escala DAPCS se describe mejor con una solución de 2 factores, tanto para padres como para madres, lo que indica la distinción entre control psicológico orientado a la dependencia y el orientado al logro. Los altos índices de consistencia interna indicaron que la escala DAPCS produjo óptimas puntuaciones. Además, la validez convergente fue confirmada por la correlación entre el DAPCS y otras medidas de control psicológico, así como con otras medidas del análisis de la crianza. La regresión jerárquica también mostró que DAPCS predijo. El autoconcepto de los adolescentes tardíos. Los resultados de este estudio indican que la versión en Español del DAPCS es un instrumento útil para estudiar ambos tipos de control psicológico

Student Perceptions of Secondary Education Teaching Effectiveness: General Profile, the Role of Personal Factors, and Educational Level.

The purpose of this study was to examine student perceptions of teaching behavior. Additionally the aim was to examine if teacher characteristics (educational level, gender, and teaching experience) could explain differences in student perceptions of their teachers. Teaching behavior was studied from the research on teaching and teacher effectiveness perspective. Secondary students (N = 7,114), taught by 410 teachers in Spain, participated in the study. Survey data were analyzed using non-parametric tests, Kruskal-Wallis, U Mann-Whitney with Bonferroni correction, and the analysis of effect sizes. Furthermore, a regression model was applied. Results showed that teaching behavior was perceived as sufficient to good, depending on the teaching behavior domain. Results indicated interesting differences between lower secondary education, upper secondary education and vocational education and training teachers. The effect size values (r U statistic) ranged between 0.43 and 0.63, highlighting the significant effect of teachers' teaching experience on the six teaching skills domains: learning climate, efficient classroom management, clarity of instruction, activating teaching, differentiation, and teaching learning strategies. Those teachers with less teaching experience were the ones who showed higher scores. Findings from the regression model showed that educational level had a significant predictive effect on the six teaching skills domains, mainly for male teachers. However, in several domains female teachers were perceived by students to outperform their male counterparts.

New validity evidence of the parent parq/control scale of parental educational styles / Nuevas evidencias de validez de la escala Parent PARQ/control sobre estilos de educación parental

BACKGROUND: The Parent PARQ/Control (short version) for mothers and fathers is an inventory of 29 items where parents record their acceptance-rejection and control behaviors towards their children. Despite vast research on IPARQTheory, it has yet to be vali-dated for a Spanish population. The goal of this study is to analyze the psychometric properties of the instrument and its factorial structure. METHOD: Participants were 4,168 parents from the Principality of Asturias (2,166 mothers and 2,002 fathers). Mean age for mothers was 39.50 years and 41.90 years for fathers. Following preliminary studies, we carried out exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) for mothers and for fathers separately. RESULTS: Analysis of mothers' responses reveal an instrument comprising 15 items with a three factor-structure: warmth/affection, hostility/aggression and control. For fathers, the structure of the inventory gave a 23 item instrument with four factors: warmth/affection, hostility/aggression, indifference/neglect and control. CONCLUSIONS: Overall, these results show that warmth/affection and control are the main dimensions in parental educational styles, and as hypothesized, mothers and fathers have different educational styles

ANTECEDENTES: el Parent PARQ/Control (versión corta, madre y padre) es un inventario de 29 ítems en el cual los progenitores reflejan las conductas de aceptación-rechazo y control que ejercen hacia los hijos e hijas. A pesar de la investigación existente en relación a la IPARQTheory, este instrumento no ha sido validado en población española. El objetivo de este estudio fue analizar las propiedades psicométricas y la estructura factorial del instrumento. MÉTODO: los participantes fueron 4.168 padres y madres del Principado de Asturias (2.166 madres y 2.002 padres) con una edad media en las madres de 39,50 y en los padres de 41,90. Siguiendo los estudios previos, se realizó un análisis factorial exploratorio y análisis factorial confirmatorio, para las madres y para los padres. RESULTADOS: en relación a la versión de la madre, se obtuvo una estructura factorial de tres factores (15 ítems): afecto, hostilidad y control. En la versión de los padres, el instrumento (23 ítems) con una estructura factorial de cuatro dimensiones: afecto, hostilidad, indiferencia y control. CONCLUSIONES: los resultados muestran que las dimensiones de afectividad y control son las principales, y que las madres y padres presentan estructuras diferentes en los estilos de educación parental

New validity evidence of the Parent PARQ/Control scale of Parental Educational Styles.

BACKGROUND: The Parent PARQ/Control (short version) for mothers and fathers is an inventory of 29 items where parents record their acceptance-rejection and control behaviors towards their children. Despite vast research on IPARQTheory, it has yet to be vali-dated for a Spanish population. The goal of this study is to analyze the psychometric properties of the instrument and its factorial structure. METHOD: Participants were 4,168 parents from the Principality of Asturias (2,166 mothers and 2,002 fathers). Mean age for mothers was 39.50 years and 41.90 years for fathers. Following preliminary studies, we carried out exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) for mothers and for fathers separately. RESULTS: Analysis of mothers’ responses reveal an instrument comprising 15 items with a three factor-structure: warmth/affection, hostility/aggression and control. For fathers, the structure of the inventory gave a 23 item instrument with four factors: warmth/affection, hostility/aggression, indifference/neglect and control. CONCLUSIONS: Overall, these results show that warmth/affection and control are the main dimensions in parental educational styles, and as hypothesized, mothers and fathers have different educational styles.