Giant-cell tumor in soft parts in a patient with osseous Paget's disease: diagnosis by fine-needle aspiration.

In this report, the cytological features and differential diagnosis of a case of giant-cell tumor (GCT) in soft tissues in a patient with osseous Paget's disease (PD) are described, with histological confirmation. Characteristic cytological findings include a diffuse cellular population of neoplastic cells composed of two types: one group of polygonal or round mononuclear small cells, exhibiting a thin-rim, dense cytoplasm and single hyperchromatic nuclei; and the second group of multinucleated, osteoclast-type giant cells with dozens of round nuclei, showing occasional micronucleoli. A prominent feature is the presence of numerous capillary structures surrounded by tumor cells. The differential diagnosis includes a number of other neoplastic and reactive processes in which giant cells may be abundant. The cytological features of GCT appear to be characteristic enough to allow a suggestive diagnosis. However, the final diagnosis should be made only after the lesion has been studied histologically.

Fluorescence In Situ Hybridization: Trisomy 12 Follow-up In Hairy Cell Leukemia.

Peripheral white blood cells from five patients with hairy cell leukemia (HCL) were studied applying fluorescence in situ hybridization (FISH) using heparinized peripheral blood, biotin-labeled chromosome 12-specific α satellite DNA probe and biotin fluorescein-labeled avidin to detect hybridization. This methodology is ideal to investigate the incidence of numerical chromosomal abnormalities of both interphase and metaphase cells. Blood samples from 28 normal subjects were included as control samples. Trisomy 12 was considered to be present when ≥4% of cells had three hybridization spots. Trisomy 12 was detected in three of the five patients at different stages of their evolution. Trisomy 12 was also evident in two patients upon relapse of the HCL. When treatment with interferon alfa (IFN) and steroids was started, clinical and analytical remission were achieved and trisomy 12 disappeared. In one patient trisomy 12 persisted regardless of treatment with IFN and a good clinical evolution. Our results show that trisomy 12 was detected in HCL with FISH at a higher frequency than that previously reported by cytogenetic analysis in either peripheral blood or in cultivated cell lines. These results indicate that the presence of trisomy 12 may be a useful marker for the presence of HCL cells, to check the percentage of trisomic cells and that trisomy 12 is also a useful marker to indicate the activity of the disease.

Fine needle aspiration biopsy diagnosis of adenoid cystic carcinoma of the breast. A case report.

A case of adenoid cystic carcinoma arising in the breast is presented. The diagnosis was established on a fine needle aspiration (FNA) biopsy sample with histopathologic corroboration. The aspirate yielded a typical smear composed of round, multilayered clusters of uniform epithelial cells arranged around cores of homogeneous, acellular material. Both the conclusiveness of FNA biopsy in diagnosing this rare mammary neoplasm and its resulting in immediate treatment are stressed.

[Short rib-polydactyly (author's transl)]. / Síndrome costilla corta-polidactilia. Variedad Saldino-Noonan. Aportación de un caso y revisión de la literatura.

A new case of short rib-polydoctyly, Saldino-Noonan type is described. The authors make a differential diagnosis between another forms of neonatal chondrodysplasias and point-out principal features of this entity.

[Cytology of urine in eosinophilic cystitis. Apropos of 19 cases]. / Estudio citológico de la orina en la cistitis eosinofila. A propósito de 19 casos.

A retrospective, cytological study has been carried out on the urine of 19 patients diagnosed by means of an endoscopic biopsy as suffering from eosinophilic cystitis. The authors have assessed the erythrocytes, the urothelial cells and the inflammatory ones, by means of a percentage count on 1,000 of the latter. In all the cases there was found to be a percentage rate of polynuclear neutrophils of more than 90% as well as the constant presence of eosinophils although in a very limited percentage and the conclusion is reached that it is impossible to diagnose eosinophil cystitis by means of the cytological study of the urine alone.