Influence of Manual and Ultrasonic Scaling on Surface Roughness of Four Different Base Materials Used to Elevate Proximal Dentin-Cementum Gingival Margins: An In Vitro Study.

AIM: To evaluate and compare the effects of both manual and ultrasonic scaling on surface roughness of four different base materials, used for elevating dentin/cementum gingival margins of proximal cavities. METHODS AND MATERIALS: Eighty human upper molars with compound Class II mesial cavities, with gingival margins 1 mm below the cemento-enamel junction (CEJ), were divided into four different groups according to the type of the base material used; resin-modified glass ionomer (RMGI), glass hybrid (HV-GIC), flowable bulk-fill resin composite (Bulk Flow) and bioactive ionic resin (Activa). This was followed by completing the restorations with the same resin composite. All materials were used according to the manufacturers' instructions. All groups were further subdivided into two subgroups according to the scaling technique: manual (hand) or ultrasonic. All restorative and scaling procedures were performed after fixation of specimens with acrylic beside neighboring teeth to simulate natural contact. The mean surface roughness (Ra, µm) of all specimens was measured quantitatively and qualitatively by a three-dimensional (3D) surface analyzer system at two stages; (1) after thermal cycling for 5000 cycles without scaling and (2) after scaling. Data were statistically analyzed using analysis of variance (ANOVA), Tukey post hoc tests, and paired sample t-tests (at α=0.05). RESULTS: For baseline readings, the Bulk Flow group had the lowest Ra values, while HV-GIC group had the highest. RMGI and Activa groups had no statistical significant difference between their Ra values (p>0.05). For post scaling readings, hand scaling had significantly lower Ra values than ultrasonic scaling in all the material groups (p<0.05), except in the Bulk Flow group, where both scaling methods were not significantly different from each other (p>0.05). CONCLUSION: Bulk Flow had the smoothest surfaces when cured against a matrix band compared with the other tested base materials. When hand and ultrasonic scaling methods were compared, the latter technique had more detrimental effect on the surface texture of the four tested base materials.

Minimally Invasive Multidisciplinary Restorative Approach to the Esthetic Zone Including a Single Discolored Tooth.

OBJECTIVES: The case report describes a minimally invasive, multidisciplinary approach to a single discolored anterior tooth, with internal bleaching using traditional Japanese paper (Washi), a gingivoplasty with a three-dimensional (3D) printed surgical guide, and ultrathin feldspathic porcelain veneers. CLINICAL CONSIDERATION: The patient's primary concern was improving her smile. After clinical evaluation, internal tooth bleaching for the discolored tooth and gingivoplasty with restoration of the maxillary anterior six teeth and first premolars was recommended. The internal tooth whitening was accomplished with sodium perborate mixed with 30% hydrogen peroxide impregnated in Washi and sealed in the root canal with glass ionomer. Once the tooth bleaching was completed, the 3D printed surgical guide was placed in the patient's maxillary anterior region and used to guide soft tissue recontouring. After 6 months, ultrathin feldspathic porcelain veneers were placed. CONCLUSION: Well-planned restorative procedures combining internal tooth bleaching using Washi, gingivoplasty performed with electrosurgery using a 3D printed surgical guide, and ultrathin feldspathic porcelain veneers can achieve the desired results in the esthetic zone and remain successful for 4 years.

Evaluation of Polishing Systems for CAD/CAM Polymer-Infiltrated Ceramic-Network Restorations.

OBJECTIVE: The purpose of this study was to evaluate the effectiveness of five different polishing systems on a computer-aided design and computer-aided manufacturing (CAD/CAM) polymer-infiltrated ceramic-network restoration with nanoscale assessment using atomic force microscopy (AFM) and visual assessment performed by dental school senior students and faculty members. METHOD: Forty-eight full coverage crowns were milled out of polymer-infiltrated ceramic-network CAD/CAM blocks (Vita Enamic) for polishing with one company proprietary, two ceramic and two composite polishing systems. The prepared crowns were divided into six groups: (1) no polishing (control); (2) polishing with Vita Enamic Polishing Kit (VEna); (3) polishing with Shofu Porcelain Laminate Polishing Kit (SCer); (4) polishing with Brasseler Dialite Feather lite All-Ceramic Adjusting & Polishing System (BCer); (5) polishing with Shofu Composite Polishing Kit (SCom); and (6) polishing with Brasseler Composite Polishing Kit (BCom). The polished crown surface topography was observed, and surface roughness and area were measured with AFM. In addition, polished crowns were visually assessed by 15 senior dental students and 15 dental school faculty members. RESULTS: All polishing treatments significantly reduced the surface roughness and area of the crown compared with the control. SCom and BCom showed significantly higher surface area than VEna, and the SCer and BCer groups were intermediate, showing no significant difference from either VEna or SCom and BCom. There were no significant differences in surface roughness between any of the systems. Dental students and faculty members classified the groups polished with VEna, SCer, and BCer groups as clinically acceptable, and they selected BCer group as the best polished restorations and the control group as the least polished restorations. CONCLUSIONS: Ceramic and composite polishing systems produced similar polishing results as that observed using a company proprietary polishing system. However, effectiveness for polishing using a company proprietary and ceramic polishing system tends to be higher than composite polishing systems.

Effect of Cervical Lesions on Fracture Resistance and Failure Mode of Maxillary Central Incisors Restored with Fiber Posts and Complete Crowns.

PURPOSE: To investigate the effect of a cervical cavity extending 1 mm apical to the cemento-enamel junction (CEJ) on fracture resistance and failure mode of maxillary central incisors that have been treated endodontically, present with complete and incomplete ferrules, and are restored with and without a fiber post. METHODS AND MATERIALS: 50 intact human maxillary central incisors were divided into five groups (n=10): CG (control group) 6-mm fer-rule height, no cervical cavity, and without post; (CO) 6-mm ferrule height without post, with a cervical cavity (access to root canal and cervical cavity restored with composite resin), cervical cavity; and post with ferrule heights of 1 mm (CP1), 2 mm (CP2), and 6 mm (CP6) restored with fiberglass post and composite resin core. After complete metal crowns were cemented on all specimens, they were subjected to thermal cycling (6000 cycles, 5°C/55°C), followed by immediate testing of fracture resistance. After failure, the specimens were sectioned buccolingually to evaluate and identify the mode of failure. The data were analyzed with an analysis of variance (ANOVA) and the Student-Newman-Keuls multiple comparison tests (α =0.05). RESULTS: A 1-mm ferrule height (CP1) fracture resistance was significantly lower (531±125 N) compared to the 6-mm ferrule height (CP6) (769±175 N) (p<0.05). With respect to the groups with similar residual dentin, with and without a cervical cavity, CG (667±119 N) and CO (668±119 N), the presence of a post (CP6) increased the resistance to fracture, although no statistically significant difference was demonstrated. Partial decementation was observed in all specimens of CG and CP6, in nine of CP1 and CP2, and in three in CO. Root fractures occurred in 23 specimens. The root surface was exposed 2 mm below the CEJ to simulate bone level. Propagation of subosseous cracks occurred in four specimens in CG and CP2, in seven specimens in CP6, in two specimens in CP1, and in six specimens in CO. All were considered catastrophic failures. CONCLUSIONS: Within the limitations of this study it is suggested that, when restoring an endodontically treated maxillary central incisor that has a cervical lesion and needs to be restored with a complete crown, a fiber post is cemented to improve fracture resistance.

Effect of Layering Techniques on Polymerization Shrinkage Stress of High- and Low-viscosity Bulk-fill Resins.

CLINICAL RELEVANCE: The use of layering techniques is still advisable with many bulk-fill resins and should be the default unless a particular resin is known to not need it. SUMMARY: Objective: The purpose of this study was to investigate how layering techniques affect polymerization shrinkage stresses of high-and low-viscosity bulk-fill resins.Method: Six high-viscosity and six low-viscosity bulk-fill resins were evaluated. Aluminum blocks with a mesial-occlusal-distal (MOD) cavity were machined and randomly divided into groups for different filling techniques (bulk-fill vs horizontal layering vs oblique layering) and further subdivided according to type of resin (high- vs low-viscosity). The cuspal deflection resulting from the polymerization of bulk-fill resin bonded to a MOD cavity within an aluminum block was measured with a digimatic micrometer. Scanning electron microscopy analyses of tested resins were also conducted.Results: In the high-viscosity bulk-fill resins, cuspal deflection of the MOD cavity ranged from 11.2 to 18.2 µm with the bulk-filling technique, from 10.7 to 15.5 µm with the horizontal layering technique, and from 10.9 to 15.2 µm with the oblique layering technique. In the low-viscosity bulk-fill resins, cuspal deflection of the material ranged from 9.2 to 19.8 µm with the bulk-filling technique, from 8.2 to 15.7 µm with the horizontal layering technique, and from 8.4 to 16.4 µm with the oblique layering technique.Conclusion: Cuspal deflections for some high-and low-viscosity bulk-fill resins were significantly reduced by using layering techniques, but the resultant improvement of layering techniques was not applicable to all the bulk-fill resins used in this study.

Salivary parameters and oral health status amongst adolescents in Mexico.

BACKGROUND: In the last years an increased interest in the use of salivary parameters in connection with caries experience and caries prediction has been shown. In schoolchildren investigations are known, where the relationship between caries prevalence and salivary parameters has been assessed, but in the adolescent population studies are scarce. The aim of the study was evaluate of the association among salivary parameters, oral health status and caries experience in adolescents in Mexico. METHODS: A cross-sectional study was conducted on 256 (DMFT≥5) and 165 (DMFT< 5) 12-to- 14-year-old adolescents. From all the adolescents, unstimulated mid-morning saliva samples were collected, after which the salivary flow rate was calculated, and the salivary pH and buffer capacity was measured. The caries was evaluated via the application of the DMFT score. Clinical variables such as oral hygiene and dental calculus were examined in the adolescent's oral cavity. The adolescents provided data on their personal characteristics by completing a questionnaire, while socioeconomic data were collected from their parents. Descriptive, bivariate and logistic regression model analyses were performed. RESULTS: The prevalence of caries was 61.1% (DMFT≥5) in permanent dentition, with 72.7% of subjects presenting poor oral hygiene. The mean levels of salivary flow rate, pH, and buffer capacity was significantly lower (p < 0.05) in adolescents with caries score of DMFT≥5 than in those with caries score of DMFT < 5. Salivary flow and buffer capacity were higher in boys than in girls. The logistic regression model applied showed that adolescents with a salivary flow rate < 1 ml per min were more likely to present caries [OR = 1.58 (CI95% 1.04-2.40); p = 0.033] than adolescents with a flow rate ≥ 1 ml per-min, and that, for each unit of increased pH, the probability of presenting caries reduced by 76% [OR = 0.24 (CI95% 0.10-0.55); p = 0.001]. CONCLUSION: Significant association was found in salivary flow rate, pH and buffer capacity in adolescents with caries (DMFT≥5). In addition to differences of these parameters by sex, the results suggest saliva parameters may act as indicators of caries in adolescents.

The effect of two composite placement techniques on fracture resistance of MOD restorations with various resin composites.

OBJECTIVE: The aim of this in vitro study was to compare the effect of two restorative placement techniques, centripetal incremental technique (CIT) and bulk-fill technique (BT) on the fracture resistance of Class II MOD restorations with various resin composites in molar teeth. MATERIALS AND METHODS: Fifty-six extracted, caries free third molars were prepared with MOD preparations and restored with resin composites. The specimens were divided into two groups by placement technique, centripetal incremental technique (CIT) and bulk-fill technique (BT). Each group was subdivided into four groups according to resin composite: hybrid (Aelite LS), nano-hybrid (Virtuoso Universal), bulk fill (Filtek One Bulk Fill) and the micro-hybrid (Herculite XRV) as the control. RESULTS: Two-way analysis of variance test (ANOVA) followed by the multiple comparison procedure, Student-Newman-Keuls Method showed no a statistically significant difference between placement techniques and fracture resistance of Class II resin composite restorations (P > 0.05). Herculite XRV resisted a significantly higher load before fracture than the other three materials at a 0.05 level of significance, while Virtuoso Universal scored the lowest load. CONCLUSIONS: There was no significant effect of the two placement techniques on the fracture resistance of Class II resin composite restorations CLINICAL SIGNIFICANCE: Resin composite restorations in Class II MODs using a simplified bulk fill placement technique showed no significant difference in fracture resistance with the centripetal technique in molar teeth.

Silver-loaded nanoparticles affect ex-vivo mechanical behavior and mineralization of dentin.

BACKGROUND: The aim was to evaluate the effect of silver loaded nanoparticles (NPs) application on the triboscopic, crystallographic and viscoelastic properties of demineralized dentin. Polymethylmetacrylate-based NPs and Ag loaded NPs were applied on demineralized dentin. MATERIAL AND METHODS: Treated and untreated surfaces were probed by a nanoindenter to test viscoelasticity, and by atomic force microscopy to test nanoroughness and collagen fibril diameter. X-ray diffraction and transmission electron microscopy through selected area diffraction and bright-field imaging were also used. RESULTS: Dentin treated with Ag-NPs attained the lowest complex modulus, and the highest tan delta values after 7 days of storage. Dentin treated with undoped-NPs achieved the lowest nanoroughness and the greatest collagen bandwidths among groups. Crystals were identified as hydroxyapatite with the highest crystallographic maturity and crystallite size in dentin treated with undoped-NPs. Texture increased in all samples from 24 h to 7 d, except in dentin surfaces treated with Ag-NPs at 310 plane. Polyhedral, block-like, hexagonal or plate-like shaped apatite crystals constituted the bulk of minerals in dentin treated with Ag-NPs, after 7 d. Polyhedral or rounded/drop-like, and polymorphic in strata crystal apatite characterized the minerals when undoped-NPs were used, with more crystalline characteristics after 7 d than that found when Ag-NPs were applied. Ag-NPs application did not improve the mechanical performance of dentin and did not produce dentin remineralization. However, energy was dissipated through the dentin without showing stress concentration; contrary was occurring at dentin treated with undoped-NPs, that provoked bridge-like mineral deposits at the dentin surface. CONCLUSIONS: Ag-NPs application did not enhance the mechanical properties of cervical dentin, though the energy dissipation did not damage the dentin structure. Remineralization at dentin was not produced after Ag-NPs application, though improved crystallinity may lead to increase stability of the apatite that was generated at the dentin surface.

The role of angiogenesis in implant dentistry part I: Review of titanium alloys, surface characteristics and treatments.

BACKGROUND: Angiogenesis plays an important role in osseointegration process by contributing to inflammatory and regenerative phases of surrounding alveolar bone. The present review evaluated the effect of titanium alloys and their surface characteristics including: surface topography (macro, micro, and nano), surface wettability/energy, surface hydrophilicity or hydrophobicity, surface charge, and surface treatments of dental implants on angiogenesis events, which occur during osseointegration period. MATERIAL AND METHODS: An electronic search was performed in PubMed, MEDLINE, and EMBASE databases via OVID using the keywords mentioned in the PubMed and MeSH headings regarding the role of angiogenesis in implant dentistry from January 2000-April 2014. RESULTS: Of the 2,691 articles identified in our initial search results, only 30 met the inclusion criteria set for this review. The hydrophilicity and topography of dental implants are the most important and effective surface characteristics in angiogenesis and osteogenesis processes. The surface treatments or modifications of dental implants are mainly directed through the enhancement of biological activity and functionalization in order to promote osteogenesis and angiogenesis, and accelerate the osseointegration procedure. CONCLUSIONS: Angiogenesis is of great importance in implant dentistry in a manner that most of the surface characteristics and treatments of dental implants are directed toward creating a more pro-angiogenic surface on dental implants. A number of studies discussed the effect of titanium alloys, dental implant surface characteristic and treatments on agiogenesis process. However, clinical trials and in-vivo studies delineating the mechanisms of dental implants, and their surface characteristics or treatments, action in angiogenesis processes are lagging.

The role of angiogenesis in implant dentistry part II: The effect of bone-grafting and barrier membrane materials on angiogenesis.

BACKGROUND: In implant dentistry, bone substitute materials and barrier membranes are used in different treatments including guided bone regeneration (GBR), socket preservation, alveolar ridge augmentation, maxillary sinus elevation, and filling bony defects around the inserted dental implant. One of the most important factors in prognosis of treatments using these materials is the growth of new blood vessels in applied areas. Present review was performed to evaluate the effect of the bone-grafting and barrier membrane materials on angiogenesis events. MATERIAL AND METHODS: An electronic search was performed in PubMed, MEDLINE, and EMBASE databases via OVID using the keywords mentioned in the PubMed and MeSH headings regarding the role of angiogenesis in implant dentistry from January 2000-April 2014. RESULTS: Of the 5,622 articles identified in our initial search results, only 33 met the inclusion criteria set for this review. Among bone substitute materials the autogenous bone-grafts, and among the barrier membranes the collagenous membranes, had the highest angiogenic potentials. Other bone-grafting materials or membranes were mostly used with pro-angiogenic factors to enhance their angiogenic properties. CONCLUSIONS: Angiogenesis is one of the key factors, which plays a critical role in success rate of GBR technique and is seriously considered in manufacturing bone-grafting and barrier membrane materials. However, there is still lack of clinical and in-vivo studies addressing the effect of angiogenesis in treatments using bone-grafting and barrier membrane materials.

Missing Concepts in De Novo Pulp Regeneration.

Regenerative endodontics has gained much attention in the past decade because it offers an alternative approach in treating endodontically involved teeth. Instead of filling the canal space with artificial materials, it attempts to fill the canal with vital tissues. The objective of regeneration is to regain the tissue and restore its function to the original state. In terms of pulp regeneration, a clinical protocol that intends to reestablish pulp/dentin tissues in the canal space has been developed--termed revitalization or revascularization. Histologic studies from animal and human teeth receiving revitalization have shown that pulp regeneration is difficult to achieve. In tissue engineering, there are 2 approaches to regeneration tissues: cell based and cell free. The former involves transplanting exogenous cells into the host, and the latter does not. Revitalization belongs to the latter approach. A number of crucial concepts have not been well discussed, noted, or understood in the field of regenerative endodontics in terms of pulp/dentin regeneration: (1) critical size defect of dentin and pulp, (2) cell lineage commitment to odontoblasts, (3) regeneration vs. repair, and (4) hurdles of cell-based pulp regeneration for clinical applications. This review article elaborates on these missing concepts and analyzes them at their cellular and molecular levels, which will in part explain why the non-cell-based revitalization procedure is difficult to establish pulp/dentin regeneration. Although the cell-based approach has been proven to regenerate pulp/dentin, such an approach will face barriers--with the key hurdle being the shortage of the current good manufacturing practice facilities, discussed herein.

Effect of thermo-mechanical loading on marginal quality and wear of primary molar crowns.

AIM: To evaluate the effect of thermo-mechanical loading (TML) on marginal quality and wear of different crown types for primary molars. METHODS: Eighty extracted human primary molars were used. After preparation, five groups received different crowns (n=16): preformed metal crowns (3M ESPE) and NuSmile crowns (Orthodontic Technologies Inc.) were inserted as preformed metal crowns; as semi-preformed crowns Protemp crowns (3M ESPE) were luted; and as individually manufactured resin composite crowns Filtek Z250 (3M ESPE) and Heliomolar (Ivoclar Vivadent) were used. Specimens were subjected to 2,500 thermal cycles between 5-55(o)C and chewing simulation for 100,000 cycles at 50N at a frequency of 0.5 Hz. Before and after thermo-mechanical loading, impressions of the teeth were taken and replicas were made. The replicas received marginal quality evaluation under a SEM at x200 magnification. Occlusal wear was measured as vertical height loss using a 3-D laser scanning microscope. RESULTS: After TML, all crowns were intact. The adhesively bonded crowns showed significantly better marginal quality to dentine/cementum compared with GIC luted crowns (p<0.05). Laboratory made crowns showed a good fit and nearly transition-free margins also after TML. Preformed metal crowns showed the significantly lowest wear rates compared to the resin composites (p<0.05). Among the resin composite groups, Protemp exhibited the most pronounced wear depths (p<0.05). CONCLUSIONS: The different crown types under investigation showed a good performance concerning the evaluated parameters marginal quality and wear.

Nanomodification of mineral trioxide aggregate for enhanced physiochemical properties.

AIM: To analyse the physicochemical properties of a Nano white mineral trioxide aggregate (NWMTA) and compare it with white mineral trioxide aggregate (WMTA). METHODOLOGY: White mineral trioxide aggregate and NWMTA were prepared and mixed according to the manufacturer's instructions. Surface area of powder before hydration, setting time, X-ray diffraction and microhardness at pH values of 4.4 and 7.4 were evaluated by Brunauer-Emmett-Teller, ISO Specification no.6876, Vickers microhardness, and energy-dispersive X-ray spectroscopy equipped with X-ray colour (dot) map for both cements. anova and Mann-Whitney were used for statistical analysis at a significance level of 0.5. RESULTS: The mean ± SD of surface area and setting time were 1.8 ± 0.2 m(2) g(-1) and 43 ± 2 min for WMTA and 7.8 ± 1.2 m(2) g(-1) and 6 ± 1 min for NWMTA, respectively. Mean ± SD of Microhardness were 16 ± 2, 51 ± 1, 69 ± 1 and 81 ± 2 for WMTA at pH values of 4.4 and 7.4 and for NWMTA correspondingly. Numbers of open porosity over the surface were 88 ± 24 and 44 ± 13 for WMTA and NWMTA, respectively. Statistical tests revealed significant differences between the groups (P < 0.001) in surface area, setting time and surface hardness for both cements. Uniform distribution of strontium was only observed in NWMTA. However, other compounds were not significantly different. CONCLUSION: Increasing surface area of powder can reduce setting time and increase microhardness even at lower pH values after hydration.

A new approach for locating the minor apical foramen using an artificial neural network.

AIM: To develop a new approach for locating the minor apical foramen (AF) using feature-extracting procedures from radiographs and then processing data using an artificial neural network (ANN) as a decision-making system. METHODOLOGY: Fifty straight single-rooted teeth were selected and placed in a socket within the alveolar bone of a dried skull. Access cavities were prepared and a file was place in the canals to determine the working length. A radiograph was taken to evaluate the location of the file in relation to the minor foramen and further checked after retrieving the tooth from the alveolar socket. The location of the file tip was categorized into: beyond the AF (long), within the root canal (short) and just at the minor AF (exact). Each radiograph was used to extract relevant features using K-means, Otsu method and Wavelet protocol. Thirty-six extracted features were used for training and the rest were used for evaluating the multi-layer Perceptron ANN model. RESULTS: Analysis of the images from radiographs (test samples) by ANN showed that in 93% of the samples, the location of the AF had been determined correctly by false rejection and acceptation error methods. CONCLUSION: Artificial neural networks can act as a second opinion to locate the AF on radiographs to enhance the accuracy of working length determination by radiography. In addition, ANN can function as a decision-making system in various similar clinical situations.

Retrospective long-term evaluation of autotransplantation of premolars to the central incisor region.

AIM: This retrospective case-series study aimed to examine the long-term outcomes of autogenously transplanted premolars. METHODOLOGY: Twelve patients in whom donor premolars were used to replace maxillary central incisors lost by trauma were clinically and radiologically monitored. Standardized clinical and radiographic records were systematically obtained during the follow-up period of 14 years, to determine the influence of specific clinical criteria on the overall success rate of transplantation. RESULTS: The success rate of premolar autotransplantation in the maxillary central incisor area was 80% after 14 years follow-up. The highest success rate occurred in those teeth transplanted with two-thirds of full root development. Complete pulp obliteration was positively related to autotransplant viability, followed by root formation in the bony crypt. CONCLUSIONS: Autotransplantation of donor teeth, at the stage of ½ to ¾ of their expected root length, can provide a successful treatment solution for over 14 years.

Microbiological analysis of gingivitis in pediatric patients under orthodontic treatment.

AIM: The aim of this study was to determine the relationship between gingival inflammation and changes in bacteria of the gingival sulcus in children in orthodontic treatment with brackets. STUDY DESIGN: this prospective study assessed gingival and plaque index of two groups: children with brackets (Group 1) and without brackets (Group 2). The sample was selected from patients treated at the Faculty of Dentistry, Complutense University of Madrid, Spain. Microbiological assessment was performed in every child and all data were statistically analysed. RESULTS: Group 1 showed significantly higher microbiological values and the difference was greater in lower teeth. Comparing the total plaque percentage, it was significantly higher in Group 1. STATISTICS: there was no significant correlation between gingival and plaque indexes in any group. No significant correlation was found between plaque index and bacteria. CONCLUSION: Children using brackets showed significantly higher gingival and plaque indices than children without brackets. No direct relationship was found between the increase in gingival and plaque indices and the presence and quantity of bacteria; therefore it was not possible to identify specific bacteria as responsible for the high gingival index in patients with brackets.

Assessment of bioactive and bio-adhesive therapies to enhance stem cell attachment to root surface dentine.

AIM: To compare bioactive and bio-adhesive therapies to enhance stem cell attachment to the root dentine of human teeth. METHODOLOGY: Dentine slabs (n = 72) were cut from the lower 3 mm of the roots of extracted human permanent teeth. The root dentine slabs were untreated, or coated with bio-adhesive, or human recombinant transforming growth factor-beta1 (hrTGF-B1), or human recombinant bone morphogenic protein-2 (hrBMP-2). The dentine slabs were placed with the root surface in contact with confluent periodontal stem cell (PSC) cultures using aseptic techniques. The cells and dentine slabs were submerged in culture media for 4, 24 and 72 h. The specimens were fixed in formalin, dehydrated and processed for scanning electron microscopy (SEM). RESULTS: SEM micrographs at x2000 magnification revealed PSC extensive adherence to root dentine for all of the bio-adhesive and bioactive treatments. The addition of bioactive molecules did not improve PSC attachment. Few cells attached to the negative control treatments. CONCLUSIONS: Bio-adhesive and bioactive growth factors were not needed to promote PSC attachment to the root dentine of human teeth, because it already appears to have good natural properties to promote PSC attachment. This suggests PSC can be used for the clinical replantation of avulsed teeth without the need for bio-adhesive and bioactive treatments.

Standards for mesiodistal and buccolingual crown size and height of primary molars in a sample of Spanish children.

AIM: The aim of this study was to obtain mesiodistal, buccolingual and height sizes of dental crowns of first and second primary molars in a sample of Spanish children given, not only their anthropological and forensic significance, but also their clinical interest for the future analysis of restorative and orthodontic treatments. MATERIALS AND METHODS: Measurements of mesiodistal, buccolingual size and height of the crowns of primary molars were studied. A transversally descriptive design was carried out and the interrelationship between these three dimensions was established. A sample of 703 first and second primary molars was selected from healthy Spanish boys and girls from 4 to 9 years old. Molars presenting anomalies of the crowns, caries or restorations were excluded. Measurements were made on dental plaster models, using a vernier caliper. Descriptive statistics and Pearson correlation coefficient was obtained and Wilcoxon's non- parametric test was applied for comparison of the mean, analysis of sexual dimorphism and comparison of antimeric teeth. RESULTS: Average sizes of each group of molars were obtained. Sexual dimorphism was studied utilizing the Wilcoxon test. A variability of relationship between the measurements was found in each molar studied. Primary second molars had less variability than first molars in the three measurements obtained. The measurement with the least variability was height, followed by mesiodistal size. CONCLUSIONS: Measurements of primary molars showed that average sizes of boys' molars were significantly larger than those of girls. Relationship between the three measurements studied was variable but second molars showed less variability than first molars. The relationship between height and width was found significant in most of the molars. i.

Comparison of operative procedure variables on pulpal viability in an ex vivo model.

AIM: To measure and compare the responses of pulp tissue to cavity preparation and restoration variables using a novel tooth slice culture model. METHODOLOGY: Experimental cavities (265) were continuously cut, under carefully controlled conditions, into the dentine of the labial aspect of 28-day-old Wistar rat incisors, and slices of these teeth maintained in organ culture for up to 2 weeks. The experimental variables examined were: the preparation method, remaining dentine thickness, coolant, drill speed, conditioning with EDTA and filling materials. The reactions of the dentine-pulp complex to the experimental variables were measured using pathohistometric analysis and the correlations between variables were determined using analysis of variance statistical tests. RESULTS: In rank order of surgically induced restorative pulpal injury, from the most to the least injurious were: remaining dentine thickness, absence of coolant during cavity preparation, bur speed, cavity conditioning treatments and the filling material. CONCLUSIONS: To reduce pulp injury and to promote pulpal repair activity, the correct use of appropriate materials are important. However, of relatively greater importance is the operative technique adopted, the need to avoid the excess removal of dentine and to minimize trauma during preparation.

Antagonist enamel wears more than ceramic inlays.

Wear phenomena of ceramic inlays are not fully understood. The aim of the present study was to evaluate ceramic wear, antagonist enamel wear, and luting cement wear over 8 years. The two-fold null hypothesis was that there would be (1) no difference in wear behavior between ceramic and enamel, and (2) no influence of filler content of luting composites on composite wear. From 96 restorations, 36 Class II inlays from 16 participants were selected. For inlays with opposing enamel cusps (n=17), replicas of inlays and enamel were scanned with a 3-D laser scanner. Luting gaps of inlays (n=36) were analyzed with a profilometer, including 3-D data analysis. Ceramic and enamel wear increased between 4 and 8 years, with significantly higher values for enamel after 6 years (p<0.05). Luting gap wear increased continuously up to 8 years (p<0.05), with no influence of luting composites (p>0.05) and location of teeth (p>0.05).