Oxidative damage in the Vesper mouse (Calomys laucha) exposed to a simulated oil spill-a multi-organ study.

Small wild mammals have been used to measure the damage caused by exposure to oil-contaminated soil, including deer mice. However, the study of toxic effects of crude oil using oxidative damage biomarkers in the wild rodent Calomys laucha (Vesper mouse) is absent. This investigation aimed to evaluate the effects of acute exposure to contaminated soil with different concentrations of crude oil (0, 1, 2, 4 and 8% w/w), simulating an accidental spill, using oxidative stress biomarkers in the liver, kidneys, lungs, testes, paw muscle, and lymphocytes of C. laucha. Animals exposed to the contaminated soil showed increases in lipid peroxidation and protein carbonylation at the highest exposure concentrations in most organ homogenates analyzed and also in blood cells, but responses to total antioxidant capacity were tissue-dependent. These results showed that acute exposure to oil-contaminated soil caused oxidative damage in C. laucha and indicate these small mammals may be susceptible to suffer the impacts of such contamination in its occurrence region, threatening the species' survival.

Emissions monitoring and carcinogenic risk assessment of PM10-bounded PAHs in the air from Candiota's coal activity area, Brazil.

The city of Candiota contains a great amount of coal resources. Coal activities, including coal combustion, are major releasers of polycyclic aromatic hydrocarbons (PAHs). The PAHs are considered priority air pollutants regarding their large carcinogenic potential. So, the carcinogenic risk assessment of populations living near areas with PAH sources is mandatory. This study aimed to evaluate the carcinogenic health risk of the PAH inhalation exposure of individuals living in Candiota City. A total of 158 individuals were enrolled in the study. Monitoring of PAH and meteorological parameters were carried out, and the health risk assessment was determined through the benzo(a)pyrene equivalent toxic equivalent quotient (BaP-TEQ) and the incremental lifetime cancer risk (ILCR) estimation. The coal activity area of Candiota demonstrated an annual PAH concentration of 27.7 ng/m3, PM10 concentration of 26.3 µg/m3, SO2 concentration of 9.5 µg/m3, a BaP-TEQ value of 0.3 ng/m3, and a daily inhalation of 62.4 ng/day. The comparison among seasons showed no difference in PAH concentration and BaP-TEQ. It was observed ILCR values of 2.8 × 10-6 and 2.6 × 10-6 for estimation based on reference and real values, respectively, and these levels were above the reference limit of 10-6, indicating cancer risk. Therefore, an epidemiological survey of cancer cases in the region and its relationship with environmental exposure and air pollutants levels must be required.

DNA damage in Brazilian newborns admitted to NICUs - association with maternal and neonatal outcomes.

Growing evidence suggests that early-life events can predispose the newborn to a variety of health problems in postnatal life, which can lead to the need for specialized care in the neonatal intensive care unit (NICU). These events may be caused by factors intrinsically related to the mother (i.e., lifestyle, socioeconomic conditions), and this interplay between maternal exposure factors and negative outcomes in the neonate can be efficiently monitored through effect biomarkers, such as DNA damage. Thus, the present study aimed to evaluate the DNA damage and the maternal and neonatal factors associated with the genotoxic outcome using newborns admitted to the NICUs of three hospitals located in the extreme south of Brazil. A total of 81 newborns were evaluated. DNA damage was assessed using the comet assay, and according to the result obtained for the evaluated parameters (tail length, % of tail DNA and tail moment). The investigation of associated factors was performed using the bivariate and multivariate Poisson regression analysis. As a result, we observed that the tail moment was the most sensitive parameter to detect differences between variables and genetic outcomes in newborns from NICU. Birthweight and the presence of respiratory diseases were associated with greater risks of DNA damage. Furthermore, the variables family income, sex, head circumference, preterm, birthweight and the presence of respiratory and/or infectious diseases showed a significant statistical difference regarding the groups with and without DNA damage (based on the median of the parameter). While the results of this study will serve as the basis for investigating genetic damage, we encourage that similar studies should be conducted elsewhere in order to confirm these and other outcomes as associated factors with DNA damage in newborns.

Lung function among residents from the largest coal region in Brazil.

Coal mining and burning activities in coal-fired power plants are among the most polluting activities in developing countries. In Brazil, the Candiota coal deposit concentrates 40% of the national mineral coal. Although, previous studies indicate several negative health outcomes to residents of this coal region, there is no information about lung function. Thus, this study aimed to evaluate lung function by spirometry among residents from the largest coal mining region of Brazil and its related factors. It was carried out a cross-sectional study with 300 male adults residing in four cities from this region. Socioeconomic, demographic, life style, and health conditions were collected through a structured questionnaire, and lung function was assessed by spirometry. Almost 18% of the residents have disorders in pulmonary function. There was significant statistical difference in the spirometry main parameters between the cities. Candiota city (host city of coal exploration activities) have the highest prevalence of obstructive ventilatory disorder. Curiously, upper economic class had significant higher risk of altered lung function (P<0.001), as well as inappropriate sewage destination (P<0.001). Residents of Candiota city had 20% higher risk of altered lung function. Regarding air quality, the PM10, SO2, and NO2 of the region were 20.6± 3.9, 7.0± 2.2, and 6.0± 1.6, respectively. Two air quality stations exceed the limit of 20 µg/m3 to PM10 proposed by Brazilian legislation and WHO, and three stations had PM10 quite close to the limit. This study points out the need for urgent action to protect residents from this coal mining region.

Oral cytological changes in young adults related to alcohol consumption.

OBJECTIVE: The study aimed to investigate the oral cytological changes in young adults with recent history of alcohol consumption, as well as its relation with the consumption of alcohol. DESIGN: The sample included 67 young adults, who performed a smear of the oral mucosa and answered a questionnaire about recent and lifetime consumption of alcohol and other drugs. The methods used were sensitive to show the association between alcohol consumption and other drugs with the damage to oral cavity cells. RESULTS: DNA fragmentation index, mitochondrial functionality and cell viability, showed a significant difference between alcohol users and nonusers. However, there was no distinction between these parameters and different frequency consumption. Alcohol consumption, economic class and use of illicit drugs were related to the cytological parameters evaluated. CONCLUSIONS: This result shows the existence of cell damages among the evaluated students and can direct future studies towards more in-depth investigations of the mechanisms involved.

Maternal, neonatal and socio-economic factors associated with intellectual development among children from a coal mining region in Brazil.

Coal is the most aggressive energy sources in the environment. Several adverse outcomes on children's health exposure to coal pollutants have been reported. Pollutants from coal power plants adversely affect the intellectual development and capacity. The present study aimed to evaluate the intellectual development and associated factors among children living a city under the direct influence (DI) and six neighboring municipalities under the indirect influence (II) of coal mining activity in the largest coal reserve of Brazil. A structured questionnaire was completed by the child's guardian, and Raven's Progressive Color Matrices were administered to each child to assess intellectual development. A total of 778 children participated. In general, no significant difference was observed between the two cities. The DI city had better socioeconomic conditions than the II municipalities according to family income (< 0.001). The prevalence of children who were intellectually below average or with intellectual disabilities was 22.9%, and there was no significant difference (p > 0.05) between municipalities. In both unadjusted and adjusted analyses, intellectual development was associated with maternal age, marital situation and maternal education level, birth weight, breast feeding, frequent children's daycare, paternal participation in children's care and child growth. Living in the DI area was not associated with intellectual disability. The results suggest that socioeconomic conditions and maternal and neonatal outcomes are more important than environmental factors for intellectual development of children living in a coal mining area.

Health risk assessment in urban parks soils contaminated by metals, Rio Grande city (Brazil) case study.

Soils and artificial surfaces of urban parks can be contaminated by toxic substances and offer risk to the human health, especially to children. Rio Grande city, southern Brazil, is a peculiar site from the point of view of environmental contamination, since the current levels of contamination reflect an accumulation of different polluting sources started in the 18th century up to the urban and industrial sources of the present. The history of Hg contamination refers to the use of Hg salts in textile activities in the 18th century and the consequent use of contaminated sediments to land urban areas. The current contamination involves metals such as Cu, Ni, Pb and Zn is related to the high degree of urbanization in the city, as well as the petrochemical and fertilizer industry. The study aimed to achieve a human health risk assessment of urban parks soils, specifically for Cu, Hg, Ni, Pb and Zn. To carry out the risk assessment using the USEPA model, three urban parks with a history of contamination were studied, using different soil exposure pathways (oral, dermal and inhalation) in the carcinogenic (Ni and Pb) and non-carcinogenic (Cu, Hg, Ni, Pb and Zn) scenarios for children and adults. The non-carcinogenic risk of Pb was found in the three urban parks studied, for children and Hg in two urban parks. Pb and Ni showed no carcinogenic risk. Even so, the high non-carcinogenic risk of Hg and Pb showed that the contamination of the past and the present are found in the urban parks of Rio Grande city, putting the health of children who use these collective spaces at risk.

Multimarker approach to assess the exposure of the wild rodent Calomys laucha to a simulated crude oil spill.

A mysterious oil spill occurred in the ocean near Brazil in 2019, which affected coastal areas in northeastern Brazil. When oil pollution occurs in coastal zones, organisms such as small mammals can suffer deleterious effects to their health. This study aimed to evaluate the effects of exposure to contaminated sandy soil with different crude oil concentrations in males of the species Calomys laucha. The exposure to crude oil resulted in multiple health issues for the subjects in the very first days of exposure. Furthermore, the exposure resulted in mutagenic damage to bone marrow blood cells and behavioral and morphological alterations, which were almost always in a dose-dependent form. The present study demonstrates the sensibility of the biomarkers used and highlights that small wild mammals such as C. laucha are useful for predicting environmental damage caused by the exposure to crude oil.

Blood markers among residents from a coal mining area.

Coal extraction and processing generate environmental contamination, which has several negative impacts on human health. Hematological and biochemical parameters are effective biomarkers of the exposure of a population to air pollutants. Thus, this study aimed to evaluate hematological and biochemical parameters in adult residents in a coal mining region in the largest national reserve in Brazil, as well as to investigate their relations with air quality. The study included three cities in the south of Brazil, which are located near an important power plant and coal mine. The air quality was analyzed by PM10, NOx, and SO2 at five air quality monitoring stations, socioeconomic and demographic characteristics were evaluated with a questionnaire, and biochemical parameters were assessed in blood samples. The mean PM10 in the study area was 19.18 µg/m3. The air quality varied among the five monitoring stations, and the Candiota station exceeded the reference limit by 63.3%. In general, participants had lived more than 10 years in the municipality and had hepatic and renal parameters within the reference values. A higher prevalence of alterations was found in hematological parameters (43.1%) and liver function (30%). Among the three cities, the population of Pedras Altas seems to have a greater impairment of the blood parameters evaluated. The only parameter that was correlated with PM10 was the hematocrit level (r = 0.33; P < 0.001).

Genotoxic risk in health-care professionals occupationally exposed to low doses of ionizing radiation.

The purpose of this study was to evaluate the potential influence of occupational ionizing radiation (IR) exposure on health professionals, assessing DNA damage using the comet and micronucleus (MN) assays and analyzing relative risks, correlations, and associated factors between outcomes and socioeconomic and lifestyle factors. Blood and buccal samples were collected from 36 workers, who actively participated in an imaging sector of a hospital, who were either exposed to IR directly or indirectly (9 internal control and 27 exposed), and 27 individuals living in the same city but with no occupational exposure (external control, unexposed/healthy). All radiation dosages performed on the 36 workers were less than 20 mSv/y, not exceeding the effective dose limit for occupational exposure. A questionnaire identified socioeconomic and lifestyle factors associated with the outcomes. The results of the MN assay showed a significant difference between both internal control and the exposed group when compared to the external control. For the comet assay, there were significant differences between the percent of tail DNA of the exposed group and external controls, but no difference was found between the exposed group and internal controls. Relative risk associations were found in time of exposure, hours worked per week, and perceived stress. Correlations were found between the outcomes and age, consumption of alcohol, and frequencies of X-rays during life. Variables that showed to be significant in the adjusted analysis were skin color and recent exposure to radiation. Albeit limited, the findings of this study suggest genotoxicity in both blood and buccal mucosa cells of workers exposed directly or indirectly to IR and that lifestyle and socioeconomic factors are associated and correlated with the risk of developing these outcomes.

Effect of mixing two environmental stressors, pH and metal contaminants, on offspring of rats exposed during gestation and lactation.

In large urban centers, the toxicity of metal mixtures may be enhanced by physicochemical factors and environmental variables, including pH. Rio Grande, a municipality located in the extreme south of Brazil, has soils with high levels of contamination due to urban and industrial activities and a high prevalence of acid rain events. Previous studies have shown that contact with elutriate of these soils can cause physiological and reproductive changes. Thus, the objective of the present study was to evaluate, through animal experimentation, the effects of a metal-contaminated soil, acidified by hydrofluoric acid at two different pH values (5.2 and 3.6), on the health of offspring of rats exposed during gestation and lactation. Female Wistar rats were gavaged daily for 42 days (gestation and breastfeeding) with soil elutriate contaminated with metals, using solvent with different pH values (6.0, 5.2, and 3.6). The following parameters were evaluated in their offspring: body and organ weight, length, appearance of developmental characteristics, and swimming. Experimental groups in which the progenitors were exposed to the solution at pH 3.6 exhibited a delayed increase in weight as well as motor deficit, with a decreased weight (onset) and length (beginning and end), while exposure in association with soil was an aggravating factor for the damages to the body. Exposure to the solution at pH 5.2 decreased the initial weight of the animals, impaired some parameters of weight development, and caused motor deficit on the 14th day. These novel findings reveal that the exposure of progenitors to environmental stressors can compromise the health of the offspring. Special attention should be given to populations living in areas with high prevalence of acid rain.

Feet in danger: short exposure to contaminated soil causing health damage-an experimental study.

In this study, hematological and behavioral changes in Wistar rats exposed to soil collected from urban areas next to an industrial complex were investigated. Animals were exposed to soil samples placed at the bottom of cages for 4 days. After this period, behavioral parameters were measured by the open field test and the elevated plus-maze. Blood was collected to measure hematological parameters. The soil from the vicinity of the oil refining industry caused changes in hematological parameters and altered behavioral parameters in both tests. The soil from the vicinity of the petroleum refining industry and fertilizer industries increased the density of white blood cells and decreased exploratory activity in the exposed animals. The results demonstrate that contact with contaminated soils, even for short periods, can cause physiological damage in organisms and that special attention should be given to people who live under constant exposure to these soils.

Antinociceptive and Anti-inflammatory Activities of Marine Sponges Aplysina Caissara, Haliclona sp. and Dragmacidon Reticulatum

ABSTRACT Marine sponges are a rich source of bioactive natural products with multiple pharmacological properties. In this study, the anti-inflammatory and antinociceptive effects of extracts obtained from Aplysina caissara, Haliclona sp. and Dragmacidon reticulatum were evaluated by using the writhing test and formalin-induced mouse paw edema model in mice. All extracts were administered via oral pathway in the doses of 60 and 90 mg/kg. In the writhing test the pre-treatment with all sponges resulted in significant inhibition of the acetic acid-induced response, suggesting an antinociceptive effect. The formalin test showed that the extracts from A. caissara, Haliclona sp. and D. reticulatum, in the tested doses, did not affect the first formalin phase, however, they were effective in the late phase. To assess the potential anti-inflammatory activity of the extracts, the test of formalin-induced paw edema was used. The oral administration of A. caissara, Haliclona sp. and D. reticulatum extracts significantly reduced the formalin-induced paw edema in mice. In conclusion, our data show that marine sponges can be an important source of anti-inflammatory and antinocicpetive products that can be promising therapeutical leads. Furthermore, pharmacological and chemical studies have been developed not only to characterize the mechanism(s) that is/are responsible for the antinociceptive and anti-inflammatory action but also to identify the active principles of sponges.

Genotoxicity in adult residents in mineral coal region-a cross-sectional study.

The present study assessed the DNA damage in environmentally exposed volunteers living in seven municipalities in an industrial coal region, through the use of the comet assay with blood cells and the micronucleus test with buccal cells. Blood and buccal smears were collected from 320 male volunteers living in seven cities inserted in a coal region. They were ages of 18 and 50 years and also completed a questionnaire intended to identify factors associated with DNA damage through a Poisson regression analysis. The comet assay detected significant differences in DNA damage in volunteers from different municipalities, and neighboring cities (Pedras Altas, Aceguá, and Hulha Negra) had a higher level of DNA damage in relation to control city. Some of the risk factors associated with identified DNA lesions included residence time and life habits. On the other hand, the micronucleus test did not identify differences between the cities studied, but the regression analysis identified risk factors such as age and life habits (consumption of mate tea and low carbohydrates diet). We conclude that there are differences in the DNA damage of volunteers from different cities of the carboniferous region, but the presence of micronuclei in the oral mucosa does not differ between the same cities. Furthermore, we alert that some related factors may increase the risk of genotoxicity, such as residence location and time, and living and food habits. Finally, we suggest the need for continuous biomonitoring of the population, as well as for investing in health promotion in these vulnerable populations.

Mutagenic effect of contaminated soil on the offspring of exposed rats / Efeito mutagênico da exposição a solos contaminados sobre a prole

This study aimed to measure irreversible DNA damage in the offspring of rats exposed to contaminated soil. Bone marrow cells were removed from the femurs of the offspring of rats that were exposed to contaminated soil via gavage during three different periods: 21 days prior to detection of pregnancy, during pregnancy and 21 days after birth, during the lactation period. For each group, we also had a control group under the same experimental conditions but exposed to uncontaminated soil. The results showed that pups borne of rats exposed to contaminated soil had an increase in the number of micronuclei in the different exposure groups compared with their respective control groups. This suggests a potential risk of mutagenic damage to the offspring of pregnant women who live at the sampling site.

Este estudo objetivou medir os danos irreversíveis de DNA na prole de ratas expostas a um solo contaminado, através do teste do micronúcleo. O mesmo foi realizado na medula óssea retirada do fêmur de filhotes de ratas expostas, via gavagem, em três diferentes períodos, ratas expostas durante 21 dias antes da detecção da prenhez, durante a prenhez e 21 dias após o nascimento, durante o período de aleitamento. Para cada grupo foi utilizado um grupo controle, mantido nas mesmas condições experimentais e expostos a um solo não contaminado. Os resultados obtidos nos filhotes expostos ao solo contaminado mostraram um aumento no número de micronúcleos nos três diferentes grupos expostos quando comparados aos seus respectivos grupos controle. Assim, os dados sugerem um potencial risco de dano mutagênico aos descendentes das gestantes residentes no local de amostragem.

Developmental effects of parental exposure to soil contaminated with urban metals.

Soil is a highly complex material, and because of rapid population growth, intense industrial activity and petrochemical development, it has suffered from contamination with substances of various origins. These environmental contaminants may have detrimental effects on human health, particularly during development. Due to the ability to transmit contaminants to the fetus, evaluating the effects of exposure of pregnant women on the psychomotor development of their offspring is of particular interest. Therefore, this study aimed to investigate the effects of exposure of female rats to an urban soil influenced by the dispersion of air contaminants during periods of pre-pregnancy, pregnancy and lactation on offspring development. Using physiological, behavioral and hematological parameters, deleterious effects on offspring were assessed. In behavioral parameters, parental exposure during pregnancy and lactation resulted in no significant differences in the evaluated parameters when compared to the control group. In contrast, pups from the pre-pregnancy group displayed decreased locomotor and exploratory activity in addition to increased levels of anxiety. Furthermore, offspring of rats exposed to contaminated urban soil during pre-pregnancy demonstrated significant changes in weight gain and development length and a reduction in the number of platelets compared to controls. Significantly, pups born to mothers exposed to contaminated urban soil during the pregnancy displayed changes in birth weight, weight gain during the growth, development length, incisor eruption and opening of the ears in addition to a reduction in their physical performance and a change in the number of lymphocytes. These results clearly show the negative influence of parental exposure to contaminated urban soil on the general development of the rats during the periods studied. These data indicate that developing organisms are highly sensitive to external factors. Further, they demonstrate the utility of these various biomarkers for identifying and displaying toxic effects of exposure to contaminated soils.

Toxic effects of the ingestion of water-soluble elements found in soil under the atmospheric influence of an industrial complex.

In this study, we investigated the toxic effects of water-soluble elements from a contaminated soil via gavage in a single dose, simulating a geophagy event. The contaminated soil was collected in a field located in an industrial complex, and the control soil was collected in a reference area. Metabolic and behavioral parameters in Wistar male rats were measured after 24 and 96 h of gavage. After 96 h, the major organs were weighed, blood was collected to check hematological parameters, the bone marrow was taken for the micronucleus test, and the liver was used for evaluating the total antioxidant capacity, lipoperoxidation and protein carbonylation. Animals exposed to contaminated soil presented a few significant alterations by comparison with control animals: TBARS and protein carbonyl levels increased, the relative weight of the kidneys increased, metabolic parameters (body weight gain, food intake, water consumption, urine and feces production) depressed and there was behavioral alteration. These findings suggest that soils impacted by atmospheric contaminants can affect the organism physiological status jeopardizing the health of populations living in industrial areas. Finally, this study reassures that ingestion of potentially contaminated soils, even for short periods of time, can cause health risks.