ABSTRACT
Studies with 6-n-propyl-2-thiouracil (PTU) in laboratory rodents have shown that transient neonatal hypothyroidism leads to increased Sertoli cell (SC) number, testis size and sperm production. However, scarce and inconclusive data are available for farm animals. In the present study, Piau pigs received PTU in a gel capsule containing 8 mg/kg of body weight for 14 weeks starting from the first week of age, whereas control animals received only the vehicle. Blood samples were collected during the experimental period for hormonal evaluation in the serum. The animals were orchiectomized at adulthood and had their testes used for histomorphometric analysis. Indicating that the PTU concentration used was effective in promoting hypothyroidism, PTU-treated pigs showed a 30% lower body weight and reduced thyroxine levels (p < 0.05) during the treatment period. At adulthood, the body weight was similar in both groups but, surprisingly, PTU-treated pigs showed 30% lower testis weight (p < 0.05). In general, treated pigs presented increased follicle-stimulating hormone levels, whereas testosterone levels tended to be lower from 9 to 23 weeks of age. No significant differences were observed for estradiol, Leydig cell volume and number, tubular diameter, SC number per gram of testis, SC efficiency and meiotic index. However, seminiferous tubule occupancy, total tubular length, SC number per testis, and daily sperm production per testis and per gram of testis (DSP/g/T) were significantly lower (p < 0.05) in PTU-treated pigs. Therefore, in contrast to laboratory rodents, our results showed that SC proliferation and DSP/g/T (spermatogenic efficiency) in Piau pigs is diminished by postnatal PTU treatment.
Subject(s)
Antimetabolites/toxicity , Hypothyroidism/pathology , Propylthiouracil/toxicity , Sertoli Cells/pathology , Spermatogenesis/drug effects , Spermatozoa/pathology , Animals , Animals, Newborn , Cell Count , Hypothyroidism/chemically induced , Leydig Cells/drug effects , Leydig Cells/pathology , Male , Seminiferous Tubules/drug effects , Seminiferous Tubules/pathology , Sertoli Cells/drug effects , Spermatozoa/drug effects , SwineABSTRACT
Avaliou-se o bagaço de cana-de-açúcar não tratado (BN) e tratado (BT) com 2, 4 e 6 por cento de hidróxido de sódio (NaOH) na dieta para coelhos em crescimento. Os coeficientes de digestibilidade (CD) dos nutrientes do BN e BT foram determinados em delineamento inteiramente casualizado, com cinco tratamentos (ração básica e rações com 40 por cento de bagaço com 0, 2, 4 e 6 por cento de NaOH) e quatro repetições. Para obtenção dos valores de CD apenas do bagaço, e não da dieta total, adotou-se o método de substituição isométrica da dieta basal proposto por Matterson et al. (1965). A adição de NaOH ao bagaço não melhorou significativamente (P>0,05) os CD da matéria seca, proteína bruta, fibra em detergente neutro, fibra bruta e energia bruta e piorou significativamente (P=0,0023) o CD da fibra em detergente ácido (y = 20,042 - 2,7615x, r² = 0,984). Concluiu-se que o tratamento do bagaço de cana-de-açúcar com NaOH não trouxe vantagens práticas sobre os valores de CD que justificassem o seu uso, já que a adoção dessa técnica demanda aumento de mão-de-obra e do custo da alimentação.
The NaOH treated sugar cane bagasse (BT) (2, 4 e 6 percent) and untreated cane bagasse (BN) was evaluated as rabbit diet ingredients. The digestion coefficients (CD) were determined in a completely randomized design, with five treatments (basic diet) and 40 percent bagasse diets (0, 2, 4 e 6 percent NaOH) and four replications, using substitution methodology (Matterson et al., 1965). The nutritive values of bagasse (DM, CP, NDF, CF and GE) were not significantly (P>0,05) affected by NaOH treatment, whereas AFD was significantly (P = 0,0023) linearly decreased by the chemical treatment (y = 20,042 - 2,7615x , r² = 0,984). It was concluded that the treatment was not effective to improving the CD of sugar cane bagasse, being the practice not justifiable.
