La aplicación de un programa de intervención para el beneficio de la salud física y emocional en mujeres adultas mayores en Hermosillo, Sonora

Se llevó a cabo un estudio cuantitativo de diseño cuasiexperimental para identificar los beneficios físicos y emocionales que promueve un programa de activación física sistematizado en mujeres adultas mayores del Instituto Nacional de las Personas Adultas Mayores (INAPAM) y del Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado de Sonora (ISSSTESON). Se aplicó para ello el Test de Senior Fitness (SFT) y la escala de ansiedad y depresión de Goldberg (EADG) a una muestra no probabilística de 70 personas de Hermosillo, Sonora del sexo femenino, mayores de 65 años (M= 68.4, DS= 5.12 años). Los resultados mostraron una disminución del 61 % en la prevalencia de ansiedad y un 30 % en el síntoma de depresión después del programa. Se observaron cambios estadísticamente significativos en la flexibilidad muscular de las extremidades inferiores (p<0.05). Respecto a la resistencia aeróbica, las participantes lograron aumentar en promedio 36 metros más de lo que habían recorrido en los 6 minutos de marcha al inicio del programa. El programa de actividad física sistematizado en mujeres adultas mayores disminuye la prevalencia de ansiedad y depresión, desarrolla la capacidad de estiramiento, capacidad aeróbica, agilidad y equilibrio dinámico.

A quantitative study of a quasi-experimental design was conducted to identify the physical and emotional benefits promoted by a systematized physical activation program in older women of the National Institute for Older Persons and the Institute of Worker Safety and Social Services of the State of Sonora. The Senior Fitness Test (SFT) and the Goldberg Anxiety and Depression Scale (GADS) were applied to a non-probabilistic sample of 70 women over 65 years old (M = 68.4, SD = 5.12 years) from Hermosillo, Sonora. The results showed a decrease of 61% in the prevalence of anxiety and a 30% decrease in the symptom of depression after the program. Statistically significant changes were observed in the muscular flexibility of the lower extremities (p <0.05). Regarding aerobic resistance, the participants managed to increase an average of 36 meters more than they had traveled in the six minutes of walking at the beginning of the program. The program of physical activity systematized in older women reduces the prevalence of anxiety and depression and develops the ability to stretch, aerobic capacity, agility, and dynamic equilibrium.

Um estudo quantitativo de desenho quase experimental para identificar os benefícios físicos e emocionais que promove um programa de activação física sistematizado em mulheres idosas do Instituto Nacional de Idosos e do Instituto de Segurança do Trabalhador e Serviços Sociais do estado de Sonora. Aplicou-se para isso o Teste de Senior Fitness (SFT) e a escala de ansiedade e depressão de Goldberg (EADG) a uma amostra não probabilística de 70 pessoas de Hermosillo, Sonora, do sexo feminino, maiores de 65 anos (M= 68.4, DS= 5.12 anos). Os resultados mostraram uma diminuição de 61% na prevalência de ansiedade e uma diminuição de 30% no sintoma de depressão após o programa. Observaram-se mudanças estatisticamente significativas na flexibilidade muscular das extremidades inferiores (p <0,05). Em relação à resistência aeróbica, as participantes conseguiram aumentar em média 36 metros a mais do que haviam percorrido nos 6 minutos de caminhada no início do programa. O programa de atividade física sistematizado em mulheres idosas diminui a prevalência de ansiedade e depressão, desenvolve a capacidade de alongamento, capacidade aeróbica, agilidade e equilíbrio dinâmico.

Yeast Biodiversity from DOQ Priorat Uninoculated Fermentations.

Climate, soil, and grape varieties are the primary characteristics of terroir and lead to the definition of various appellations of origin. However, the microbiota associated with grapes are also affected by these conditions and can leave a footprint in a wine that will be part of the characteristics of terroir. Thus, a description of the yeast microbiota within a vineyard is of interest not only to provide a better understanding of the winemaking process, but also to understand the source of microorganisms that maintain a microbial footprint in wine from the examined vineyard. In this study, two typical grape varieties, Grenache and Carignan, have been sampled from four different vineyards in the DOQ Priorat winegrowing region. Afterward, eight spontaneous alcoholic fermentations containing only grapes from one sampling point and of one variety were conducted at laboratory scale. The fermentation kinetics and yeast population dynamics within each fermentation experiment were evaluated. Yeast identification was performed by RFLP-PCR of the 5.8S-ITS region and by sequencing D1/D2 of the 26S rRNA gene of the isolates. The fermentation kinetics did not indicate clear differences between the two varieties of grapes or among vineyards. Approximately 1,400 isolates were identified, exhibiting high species richness in some fermentations. Of all the isolates studied, approximately 60% belong to the genus Hanseniaspora, 16% to Saccharomyces, and 11% to Candida. Other minor genera, such as Hansenula, Issatchenkia, Kluyveromyces, Saccharomycodes, and Zygosaccharomyces, were also found. The distribution of the identified yeast throughout the fermentation process was studied, and Saccharomyces cerevisiae was found to be present mainly at the end of the fermentation process, while Aureobasidium pullulans was isolated primarily during the first days of fermentation in three of the eight spontaneous fermentations. This work highlights the complexity and diversity of the vineyard ecosystem, which contains yeasts from different species. The description of this yeast diversity will lead to the selection of native microbiota that can be used to produce quality wines with the characteristics of the Priorat.

Fungal diversity in grape must and wine fermentation assessed by massive sequencing, quantitative PCR and DGGE.

The diversity of fungi in grape must and during wine fermentation was investigated in this study by culture-dependent and culture-independent techniques. Carignan and Grenache grapes were harvested from three vineyards in the Priorat region (Spain) in 2012, and nine samples were selected from the grape must after crushing and during wine fermentation. From culture-dependent techniques, 362 isolates were randomly selected and identified by 5.8S-ITS-RFLP and 26S-D1/D2 sequencing. Meanwhile, genomic DNA was extracted directly from the nine samples and analyzed by qPCR, DGGE and massive sequencing. The results indicated that grape must after crushing harbored a high species richness of fungi with Aspergillus tubingensis, Aureobasidium pullulans, or Starmerella bacillaris as the dominant species. As fermentation proceeded, the species richness decreased, and yeasts such as Hanseniaspora uvarum, Starmerella bacillaris and Saccharomyces cerevisiae successively occupied the must samples. The "terroir" characteristics of the fungus population are more related to the location of the vineyard than to grape variety. Sulfur dioxide treatment caused a low effect on yeast diversity by similarity analysis. Because of the existence of large population of fungi on grape berries, massive sequencing was more appropriate to understand the fungal community in grape must after crushing than the other techniques used in this study. Suitable target sequences and databases were necessary for accurate evaluation of the community and the identification of species by the 454 pyrosequencing of amplicons.

Overcoming soft-tissue deficiency in toe-to-hand transfer using a dorsalis pedis fasciosubcutaneous toe free flap: Surgical technique.

Reconstruction of combined finger and soft-tissue defects poses a technical surgical challenge. We present our experience with a hybrid flap: the dorsalis pedis fasciosubcutaneous-toe free flap. In a single stage, this flap solves the problem of medium-sized defects associated with digit losses in the hand. Donor-site morbidity has been minimal.