ABSTRACT
Coronavirus disease 2019 (COVID-19) is the latest respiratory pandemic resulting from zoonotic transmission of severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2). Severe symptoms include viral pneumonia secondary to infection and inflammation of the lower respiratory tract, in some cases causing death. We developed primary human lung epithelial infection models to understand responses of proximal and distal lung epithelium to SARS-CoV-2 infection. Differentiated air-liquid interface cultures of proximal airway epithelium and 3D organoid cultures of alveolar epithelium were readily infected by SARS-CoV-2 leading to an epithelial cell-autonomous proinflammatory response. We validated the efficacy of selected candidate COVID-19 drugs confirming that Remdesivir strongly suppressed viral infection/replication. We provide a relevant platform for studying COVID-19 pathobiology and for rapid drug screening against SARS-CoV-2 and future emergent respiratory pathogens. ONE SENTENCE SUMMARY: A novel infection model of the adult human lung epithelium serves as a platform for COVID-19 studies and drug discovery.
ABSTRACT
The pathogenesis of idiopathic pulmonary fibrosis (IPF) is probably the result of interplay between cytokines/chemokines and growth factors. The renin-angiotensin (Ang) system is involved, although its profibrotic effect is attributed to Ang II. However, recent studies suggest that renin, through a specific receptor, is implicated in fibrogenesis. In this study, the expression of renin and renin receptor was examined in normal and IPF lungs and fibroblasts. Normal human lung fibroblasts were stimulated with renin or transfected with renin small interfering RNA (siRNA), and the expression of transforming growth factor (TGF)-ß1 and α-1-type I collagen was analysed. Normal lungs and lung fibroblasts expressed renin, which was strongly upregulated in IPF lungs and fibroblasts (â¼10-fold increase; p<0.05). Immunocytochemistry showed intense renin staining in IPF fibroblasts. Renin-stimulated lung fibroblasts displayed an increase in the expression of TGF-ß1 (mean ± sd 1.8 × 10(3) ± 0.2 × 10(3) versus 1.2 × 10(3)± 0.3 × 10(3) mRNA copies per 18S ribosomal RNA; p<0.01) and collagen (5.93 × 10(2)± 0.66 × 10(2) versus 3.28 × 10(2) ± 0.5 × 10(2); p<0.01), while knocking down renin expression using siRNA provoked a strong decrease of both molecules. These effects were independent of Ang II, since neither losartan nor captopril decreased these effects. Renin also decreased matrix metalloprotease-1 expression and induced TGF-ß1 activation (163 ± 34 versus 110 ± 15 pg active TGF-ß1 per mg total protein). These findings highlight the possible role of renin as an Ang II-independent profibrotic factor in lung fibrosis.
Subject(s)
Angiotensins/metabolism , Lung/metabolism , Pulmonary Fibrosis/metabolism , Renin/blood , Cells, Cultured/cytology , Collagen/metabolism , Fibroblasts/cytology , Fibrosis , Gene Expression Regulation , Humans , Matrix Metalloproteinase 2/metabolism , RNA, Small Interfering/metabolism , Recombinant Proteins/metabolism , Renin/biosynthesis , Renin-Angiotensin System , Transforming Growth Factor beta1/metabolismSubject(s)
Hypertension/epidemiology , Adolescent , Adult , Aged , Female , Humans , Hypertension/therapy , Male , Mexico , Middle Aged , Rural PopulationABSTRACT
El presente estudio da a conocer prevalencia y algunas caracteristicas epidemiologicas de una poblacion de 1.564 personas con cifras de presion altas, utilizando tres tipos de criterio. Se observo una prevalencia declarada de 7,7% en hombres y de 10,8% en mujeres y una prevalencia real que varia segun el criterio utilizado y el sexo. Las mayores frecuencias estuvieron en las cifras altas de presion distolica segun el criterio de la OMS. Se discute la influencia del factor sobrepeso y bajo peso asociado a cifras altas de presion arterial
