A Novel Marine Mammal Coxiella burnetii-Genome Sequencing Identifies a New Genotype with Potential Virulence.

The obligate intracellular bacterial pathogen Coxiella burnetii has been identified in a few species of marine mammals, some of which are showing population declines. It has been hypothesized that C. burnetii in marine mammals is a distinct genotype that varies significantly from the typical terrestrial genotypes. It appears to lack an IS1111. Isolates originating from Australian marine animals have a distinctly non-Australian profile of multiple-locus variable-number tandem-repeat analysis (MLVA). Extracted Coxiella DNA of Australian fur seal placental origin was sequenced using the Novaseq platform. Illumina 150 bp paired-end reads were filtered and trimmed with Trimgalore. The microbial community present in the sequenced genome was evaluated with Kraken and Bracken software using the NCBI database. A phylogenetic analysis was performed using 1131 core genes. Core genes were identified using Panaroo and inputted into Iqtree to determine the maximum-likelihood tree. A second phylogenetic tree was created using Rickettsiella grylii and using seven housekeeping genes. Results were compared with the C. burnetii Nine Mile RSA439 virulent genome. This new Australian marine mammal isolate of Coxiella (PG457) appears to be a novel genotype that lacks IS1111 and has a distinct MLVA signature (ms26, ms27, ms28, ms30, and ms31). The presence of genes for multiple virulence factors appears to give this genotype sufficient pathogenicity for it to be considered a possible causative agent of abortion in Australian fur seals as well as a potential zoonotic risk.

Perceptions of blind proficiency testing among latent print examiners.

In recent years, scholars have levied multiple criticisms against traditional proficiency testing procedures in forensic laboratories. Consequently, on several occasions, authorities have formally recommended that laboratories implement blind proficiency testing procedures. Implementation has been slow, but laboratory management has increasingly expressed interest in initiating blind testing in at least some forensic disciplines, with some laboratories conducting blind testing in almost all disciplines. However, little is known about how a key population perceives blind proficiency testing, i.e., forensic examiners. We surveyed active latent print examiners (N = 338) to explore perceptions of blind proficiency testing and determine whether beliefs varied between examiners who work for laboratories with and without blind proficiency testing. Results suggest that examiners do not hold particularly strong beliefs about such procedures, but that examiners who work in laboratories with blind proficiency testing procedures view them significantly more positively than those who do not. Further, examiner responses provide insight into potential obstacles to continued implementation.

What types of information can and do latent print examiners review? A survey of practicing examiners.

Understanding typical work practices is important to understanding the decision-making process underlying latent print comparison and improving the reliability of the discipline. Despite efforts to standardize work practices, a growing literature has demonstrated that contextual effects can influence every aspect of the analytic process. However, very little is known about what types of information are available to latent print examiners, and what types of information latent print examiners routinely review. We surveyed practicing latent print examiners (N = 284) regarding what types of information are accessible during routine casework, and what types of information they routinely review during casework. We also explored whether access and inclination to review different types of information vary according to unit size and examiner role. Results indicated that information describing the physical evidence is accessible by almost all examiners (94.4%), and most examiners have access to offense type (90.5%), method of evidence collection (77.8%), and the names of both suspect (76.1%) and victim (73.9%). However, evidence description (86.3%) and method of evidence collection (68.3%) were the only information types consistently reviewed by most examiners. Findings also indicate that examiners in smaller laboratories have access to more information types and often review more information types than examiners from larger laboratories, but both populations choose to not review information at similar rates. Further, examiners in supervisory positions are more likely to choose to not review information than examiners in non-supervisory positions. Although there is some consensus regarding what types of information examiners routinely review, findings suggest that there is little absolute consensus regarding what information examiners can even access, and highlight two sources of variability in examiner work practices: employment setting and examiner role. This is concerning in light of efforts to maximize the reliability of analytic procedures (and ultimately, conclusions) and represents an important area of future study as the field progresses.

Pacific Gulls (Larus pacificus) as Potential Vectors of Coxiella burnetii in an Australian Fur Seal Breeding Colony.

Recently, Coxiella burnetii has been described as a novel pathogen potentially contributing to decreased pup production in Australian fur seals (AusFS, Arctocephalus pusillus doriferus). Pacific gulls (PGs, Larus pacificus) are known to scavenge AusFS placental material during the fur seal breeding season. It is hypothesized that PGs may act as vectors for this pathogen. In the present study, cloacal swabs, oral swabs and serum were collected from PGs on Kanowna Island (KI, an AusFS breeding colony) and a nearby island, Seal Island (SI), not occupied by pinnipeds. All sample sets were evaluated with qPCR for the com1, htpAB and IS1111 markers. Most oral and cloacal swabs from KI tested positive on both the com1 (94.1%; 88.2%) and htpAB targets (76.5%; 76.5%). Amplification was very low from the SI oral swabs and cloacal swabs. Only the KI serum samples had amplification (17.7% for both com1 and htpAB). There was no IS1111 amplification in either colony. The results demonstrate that PGs can potentially act as vectors for the spread of C. burnetii. In some birds, C. burnetii was detectable in the serum, indicating that gulls can experience bacteraemia. It appears that different feeding strategies in the same species within the same ecosystem can have profound effects on the prevalence of pathogens. Further studies are required to better understand the epidemiology and potential risks of this organism.

Does image editing improve the quality of latent prints? An analysis of image-editing techniques in one crime laboratory.

Field research within latent print comparison has remained sparse in the context of an otherwise growing body of literature examining the discipline. Studies examining how ACE-V procedures are implemented within active crime laboratories are especially lacking in light of research suggesting significant variability in examiner practices despite standardized ACE-V procedures. To date, no studies have examined a potentially important aspect of the Analysis phase: digital image editing. We provide information on the prevalence and types of latent print image editing within one laboratory (i.e., Houston Forensic Science Center), examine the potential effect of image editing on objective print quality and clarity (i.e., LQMetrics scores), and explore potential examiner differences in editing effectiveness. Results indicate that most latent prints are edited in some manner, and that image editing improves the quality and clarity of print images as defined by an objective quality metric, although examiners varied in their ability to improve the clarity of print images. Findings suggest that formal guidance or documentation of standard editing procedures would likely improve the reliability of examiner conclusions early in the latent print comparison process.

Gun violence among young adults with a juvenile crime record in North Carolina: Implications for firearm restrictions based on age and risk.

Youth who acquire a juvenile crime record may be at increased risk of perpetrating gun violence as adults. North Carolina and 22 other states permit young adults who were adjudicated by a juvenile court - even for some felony-equivalent offenses - to legally access firearms. Effectiveness of gun restrictions for adults with juvenile crime histories has not been systematically studied. This article reports findings from a longitudinal study of arrests and convictions for gun-involved and other offenses in 51,059 young adults in North Carolina, comparing those with gun-disqualifying and not-disqualifying juvenile records. The annualized rate of arrest for gun-involved crime in those with a felony-level juvenile record was 9 times higher than the rate of reported comparable offenses in the same age group in the North Carolina general population (3349 vs. 376 per 100,000). Among those with a felony-equivalent juvenile delinquency adjudication who became legally eligible to possess firearms at age 18, 61.8% were later arrested for any criminal offense, 14.3% for a firearm-involved offense. Crimes with guns were most likely to occur among young adults who had committed more serious (felony or equivalent) offenses before age 18; had been adjudicated at younger ages; acquired a felony conviction as a youth; and spent time in prison. The prevalence of arrests for crimes involving guns among young adults in North Carolina with a gun-disqualifying felony record acquired before age 18 suggests that the federal gun prohibitor conferred by a felony record is not highly effective as currently implemented in this population. From a risk-based perspective, these restrictions appear to be justified; better implementation and enforcement may improve their effectiveness. Gun crime prevention policies and interventions should focus on these populations and on limiting illegal access to firearms.

MEDETOMIDINE-KETAMINE-BUPRENORPHINE ANESTHESIA OF THE SOLITARY SUBTERRANEAN CAPE DUNE MOLE-RAT (BATHYERGUS SUILLUS) AND THE CAPE MOLE-RAT (GEORYCHUS CAPENSIS).

A prospective, descriptive study was conducted to evaluate the safety and efficacy of a field-ready anesthetic drug combination of medetomidine-ketamine-buprenorphine for data logger implantation surgery or recheck in free-ranging Cape dune (Bathyergus suillus: n = 41) and Cape (Georychus capensis: n = 37) mole-rats. All anesthesia data were reported as mean (±standard deviation). Medetomidine-ketamine-buprenorphine doses were 0.1 (±0.03), 10.6 (±2.8), and 0.06 (±0.03) mg/kg, respectively, for Cape dune mole-rats; and 0.2 (±0.03), 19.4 (±4.0), and 0.14 (±0.03) mg/kg, respectively, for Cape mole-rats. Induction was calm and took 2.00 (range: 1.00-6.00) min for the Cape dune and 1.75 (range 1.25 to 8.16) min for Cape mole-rats. A surgical plane of anesthesia was achieved in most Cape dune mole-rats (92%) and Cape mole-rats (90%). The remainder required supplementation with a single intramuscular injection of ketamine (3-9 mg/kg) during surgery. Heart and respiratory rates were 149 (±37) beats and 24 (±8) breaths per minute, respectively, for Cape dune mole-rats and 179 (±40) beats and 25 (±10) and breaths per minute, respectively for Cape mole-rats. Surgical time for mole-rats ranged from 25 to 38 min. Recovery was calm and took 8.50 (range: 2.00-19.00) min for Cape dune mole-rats and 9.75 (range: 2.00-34.00) min for Cape mole-rats to recover. For recovery, atipamezole was administered intramuscularly at 0.5 (±0.15) mg/kg for Cape dune mole-rats and 1 (±0.15) mg/kg for Cape mole-rats. All mole-rats were returned to their original burrows within 48 h of recovery. The medetomidine-ketamine-buprenorphine combination induced a predictable, safe anesthesia in Cape dune and Cape mole-rats suitable for short intraabdominal surgery. This combination is suited to in situ studies where the use of a formal surgery or laboratory is not feasible.

Disentanglement of Cape fur seals (Arctocephalus pusillus pusillus) with reversible medetomidine-midazolam-butorphanol.

Anaesthesia in pinnipeds is considered a much higher risk than in most terrestrial mammals because of their frequent proximity to water and physiological and anatomical adaptations related to diving, which also influence their anaesthesia management. Anaesthetising and immobilising entangled seals does not allow for selection of animals that are at a safe distance from the water's edge. Medetomidine-midazolam-butorphanol (MMB) sedation was trialled on eight entangled Cape fur seals (CFS) (Arctocephalus pusillus pusillus) to determine if it was safe to use on animals that entered the water post-darting. The MMB was given at an estimated dose of 0.03 mg/kg, 0.2 mg/kg and 0.2 mg/kg, respectively, via remote darting. Sedation was reversed with intramuscular atipamezole (0.15 mg/kg) and naltrexone (0.4 mg/kg) to antagonise the effects of medetomidine and butorphanol, respectively. Moderate sedation was achieved in six animals. Six of the animals entered the water after being darted. There was a single mortality and a single animal that was too lightly sedated for capture. The preliminary results indicate that MMB produces suitable sedation for disentanglement of CFS. Additionally, MMB might be suitable for application to field-based biological research.

Latent print quality in blind proficiency testing: Using quality metrics to examine laboratory performance.

Calls for blind proficiency testing in forensic science disciplines intensified following the 2009 National Academy of Sciences report and were echoed in the 2016 report by the President's Council of Advisors on Science and Technology. Both practitioners and scholars have noted that "open" proficiency tests, in which analysts know they are being tested, allow for test-taking behavior that is not representative of behavior in routine casework. This study reports the outcomes of one laboratory's blind quality control (BQC) program. Specifically, we describe results from approximately 2.5 years of blind cases in the latent print section (N = 376 latent prints submitted as part of 144 cases). We also used a widely available quality metrics software (LQMetrics) to explore relationships between objective print quality and case outcomes. Results revealed that nearly all BQC prints (92.0%) were of sufficient quality to enter into AFIS. When prints had a source present in AFIS, 41.7% of print searches resulted in a candidate list containing the true source. Examiners committed no false positive errors but other types of errors were more common. Average print quality was in the midpoint of the range (53.4 on a 0-to-100 scale), though prints were evenly distributed across the Good, Bad, and Ugly categories. Quality metrics were significantly associated with sufficiency determinations, examiner conclusions, and examiner accuracy. Implications for blind testing and the use of quality metrics in routine casework as well as proficiency testing are discussed.

Judges and forensic science education: A national survey.

In criminal cases, forensic science reports and expert testimony play an increasingly important role in adjudication. More states now follow a federal reliability standard, which calls upon judges to assess the reliability and validity of scientific evidence. Little is known about how judges view their own background in forensic scientific evidence, and what types of specialized training they receive on it. In this study, we surveyed 164 judges from 39 different U.S. states, who attended past trainings at the National Judicial College. We asked these judges about their background in forensic science, their views concerning the reliability of common forensic disciplines, and their needs to better evaluate forensic science evidence. We discovered that judges held views regarding the scientific support for different forensic science disciplines that were fairly consistent with available literature; their error rate estimates were more supported by research than many estimates by laypersons, who often assume forensic methods are nearly infallible. We did not find any association between how judges rate forensic reliability and prior training. We did, however, find that training corresponded with judges' views that they should, and do in fact, take on a more active gatekeeping role regarding forensics. Regarding the tools judges need to vet forensic experts and properly evaluate forensic science evidence, they reported having very different backgrounds in relevant scientific concepts and having forensic science education needs. Judges reported needs in accessing better material concerning reliability of forensic science methods. These results support new efforts to expand scientific evidence education in the judiciary.

Are southern African solitary mole-rats homeothermic or heterothermic under natural field conditions?

Abandoning of a stable body temperature (Tb), a phenomenon known as heterothermy, is an adaptation to cope mainly with a lack of food and water, especially in species inhabiting daily or seasonally variable environments. There is increasing evidence that African mammals avoid adverse conditions by heterothermy and eventually by entering torpor. Members of subterranean rodent family, the African mole-rats (Bathyergidae), are suitable candidates to study both phenomena, because of the diversity of their strategies in respect of maintaining stable Tb ranging from homeothermic species to a mammal with the most labile Tb, the naked mole-rat. Currently, there are field data on daily and seasonal Tb in one social species only and such information are lacking for any solitary mole-rat. In our study, we recorded yearly Tb in two solitary bathyergids, the Cape mole-rat Georychus capensis and the Cape dune mole-rat Bathyergus suillus from South Africa using intraperitoneally implanted dataloggers. Since this region is characterised by changing ecological characteristics, we expected either decreases of Tb within 24 h indicating daily torpor and/or longer-term decreases of Tb, which would indicate multiday torpor. Although we found seasonally phase shifted low amplitude daily Tb cycles, we did not find any remarkable and regular daily and/or seasonal Tb deviations, likely showing an absence of torpor in both species. Due to absence of this energy saving mechanism, we may speculate that both species could be vulnerable to ongoing global climatic change.

Latent print comparison and examiner conclusions: A field analysis of case processing in one crime laboratory.

Scholarship on the latent print comparison process has expanded in recent years, responsive to the call for rigorous research by scholarly groups (e.g., National Academy of Sciences, 2009; President's Council of Advisors on Science and Technology, 2016). Important to the task of ultimately improving accuracy, consistency, and efficiency in the field is understanding different workflows and case outcomes. The current study describes the casework completed by a latent print unit in a large laboratory during one calendar year (2018), including a unique workflow that involves Preliminary AFIS Associations reported out as investigative leads. Approximately 45% of all examined prints were deemed to be of sufficient quality to enter into AFIS, and 22% of AFIS entries resulted in potential identifications. But examiner conclusions and AFIS outcomes (across three AFIS databases) varied according to case details, print source, and AFIS database. Moreover, examiners differed in case processing, sufficiency determinations, and AFIS conclusions. Results are discussed with respect to implications for future research (e.g., comparing these data to case processing data for other laboratories) and ultimately improving the practice of latent print examination.

Disease risk analysis in sea turtles: A baseline study to inform conservation efforts.

The impact of a range of different threats has resulted in the listing of six out of seven sea turtle species on the IUCN Red List of endangered species. Disease risk analysis (DRA) tools are designed to provide objective, repeatable and documented assessment of the disease risks for a population and measures to reduce these risks through management options. To the best of our knowledge, DRAs have not previously been published for sea turtles, although disease is reported to contribute to sea turtle population decline. Here, a comprehensive list of health hazards is provided for all seven species of sea turtles. The possible risk these hazards pose to the health of sea turtles were assessed and "One Health" aspects of interacting with sea turtles were also investigated. The risk assessment was undertaken in collaboration with more than 30 experts in the field including veterinarians, microbiologists, social scientists, epidemiologists and stakeholders, in the form of two international workshops and one local workshop. The general finding of the DRA was the distinct lack of knowledge regarding a link between the presence of pathogens and diseases manifestation in sea turtles. A higher rate of disease in immunocompromised individuals was repeatedly reported and a possible link between immunosuppression and environmental contaminants as a result of anthropogenic influences was suggested. Society based conservation initiatives and as a result the cultural and social aspect of interacting with sea turtles appeared to need more attention and research. A risk management workshop was carried out to acquire the insights of local policy makers about management options for the risks relevant to Queensland and the options were evaluated considering their feasibility and effectiveness. The sea turtle DRA presented here, is a structured guide for future risk assessments to be used in specific scenarios such as translocation and head-starting programs.

The impact of misdemeanor arrests on forensic mental health services: A state-wide review of Virginia sanity evaluations.

OBJECTIVE: We examined the prevalence of criminal defendants facing only misdemeanor charges and referred for forensic mental health evaluations of legal sanity (criminal responsibility) in a state-wide sample of sanity reports. We sought to describe this population of defendants, particularly as compared to defendants facing felony charges and referred for evaluation of legal sanity. HYPOTHESES: We hypothesized that, among those referred for sanity evaluations, defendants facing only misdemeanor charges would have higher rates of serious mental illness than would defendants charged with felonies, as evidenced by their mental status during the evaluation and at the time of alleged offenses. We also hypothesized that defendants charged with only misdemeanors would be more often opined insane, as compared to those charged with felonies. METHOD: We reviewed a statewide sample of 926 court-ordered sanity evaluation reports in Virginia and coded numerous variables describing the defendants, sanity evaluation process, resulting reports, and legal opinions offered within the reports. RESULTS: Approximately 22.3% of sanity evaluations involved defendants charged only with misdemeanor offenses. Defendants facing only misdemeanor charges were 1.82 times more likely to be opined insane than were defendants facing only felony charges, primarily due to their increased likelihood of experiencing psychotic symptoms at the time of the offense (1.83 times more likely than defendants facing felony charges). CONCLUSIONS: The merits of pursuing the insanity defense in response to misdemeanor charges are questionable given the cost- and resource-intensive outcomes associated with insanity acquittals. Diversion strategies may be a more efficient response to those defendants with serious mental illness facing only misdemeanor charges. (PsycInfo Database Record (c) 2020 APA, all rights reserved).

How do latent print examiners perceive proficiency testing? An analysis of examiner perceptions, performance, and print quality.

Proficiency testing has the potential to serve several important purposes for crime laboratories and forensic science disciplines. Scholars and other stakeholders, however, have criticized standard proficiency testing procedures since their implementation in laboratories across the United States. Specifically, many experts label current proficiency tests as non-representative of actual casework, at least in part because they are not sufficiently challenging (e.g., [1-4]. In the current study, we surveyed latent print examiners (n = 322) after they completed a Collaborative Testing Services proficiency test about their perceptions of test items. We also evaluated respondents' test performance and used a quality metric algorithm (LQMetrics) to obtain objective indicators of print quality on the test. Results were generally consistent with experts' concerns about proficiency testing. The low observed error rate, examiner perceptions of relative ease, and high objective print quality metrics together suggest that latent print proficiency testing is not especially challenging. Further, examiners indicated that the test items that most closely resembled real-world casework were also the most difficult and contained prints of the lowest quality. Study findings suggest that including prints of lower quality may increase both the difficulty and representativeness of proficiency testing in latent print examination.

Competency to stand trial evaluations: A state-wide review of court-ordered reports.

Competence to stand trial (CST) evaluations are a critical part of certain criminal proceedings, and competence-related evaluation and treatment are an increasing part of public mental health services. Whereas more research describes the defendants undergoing competence evaluations, less research has examined the actual reports detailing those competence evaluations. This study reviewed 3,644 court-ordered CST evaluation reports submitted by 126 evaluators in Virginia since Virginia initiated an oversight system allowing for comprehensive review. The base rate of incompetence opinions was 38.8%, but these rates varied significantly across evaluation type (initial versus post-restoration efforts) and evaluators (ranging from 9.1% to 76.8% incompetence rate). Results suggest generally strong compliance with state statutes guiding CST evaluations, but also highlight marked variability in forensic conclusions and reveal a few areas in which some reports fell short of statutory requirements and practice guidelines.

Latent Print Proficiency Testing: An Examination of Test Respondents, Test-Taking Procedures, and Test Characteristics.

Proficiency testing is a key component of quality assurance programs within crime laboratories and can help improve laboratory practices. However, current proficiency testing procedures contain significant limitations and can be misinterpreted by examiners and court personnel (Garrett & Mitchell, 2018). To evaluate some of these limitations, we surveyed latent print examiners (n = 198) after they completed a Collaborative Testing Services, Inc. proficiency test. Additionally, we evaluated test performance and used a quality metric algorithm to evaluate the quality of test prints. Results do not suggest that respondents are dissimilar to the broader examiner population, although they may engage in different behaviors when completing tests versus casework. Findings show that proficiency testing contains prints of high quality and is perceived as both relatively easy and representative of casework. The test discriminated between inexperienced and experienced respondents, and verification procedures were largely ineffective in reducing errors. Objective quality metrics may provide a path forward to improving proficiency testing in a measurable manner.

What do forensic analysts consider relevant to their decision making?

In response to research demonstrating that irrelevant contextual information can bias forensic science analyses, authorities have increasingly urged laboratories to limit analysts' access to irrelevant and potentially biasing information (Dror and Cole (2010) [3]; National Academy of Sciences (2009) [18]; President's Council of Advisors on Science and Technology (2016) [22]; UK Forensic Science Regulator (2015) [26]). However, a great challenge in implementing this reform is determining which information is task-relevant and which is task-irrelevant. In the current study, we surveyed 183 forensic analysts to examine what they consider relevant versus irrelevant in their forensic analyses. Results revealed that analysts generally do not regard information regarding the suspect or victim as essential to their analytic tasks. However, there was significant variability among analysts within and between disciplines. Findings suggest that forensic science disciplines need to agree on what they regard as task-relevant before context management procedures can be properly implemented. The lack of consensus about what is relevant information not only leaves room for biasing information, but also reveals foundational gaps in what analysts consider crucial in forensic decision making.

Perceptions and estimates of error rates in forensic science: A survey of forensic analysts.

Every scientific technique features some error, and legal standards for the admissibility of scientific evidence (e.g., Daubert v. Merrill Dow Pharmaceuticals, Inc., 1993; Kumho Tire Co v. Carmichael, 1999) guide trial courts to consider known error rates. However, recent reviews of forensic science conclude that error rates for some common techniques are not well-documented or even established (e.g., NAS, 2009; PCAST, 2016). Furthermore, many forensic analysts have historically denied the presence of error in their field. Therefore, it is important to establish what forensic scientists actually know or believe about errors rates in their disciplines. We surveyed 183 practicing forensic analysts to examine what they think and estimate about error rates in their various disciplines. Results revealed that analysts perceive all types of errors to be rare, with false positive errors even more rare than false negatives. Likewise, analysts typically reported that they prefer to minimize the risk of false positives over false negatives. Most analysts could not specify where error rates for their discipline were documented or published. Their estimates of error in their fields were widely divergent - with some estimates unrealistically low.

Do evidence submission forms expose latent print examiners to task-irrelevant information?

Emerging research documents the ways in which task-irrelevant contextual information may influence the opinions and decisions that forensic analysts reach regarding evidence (e.g., Dror and Cole, 2010; National Academy of Sciences, 2009; President's Council of Advisors on Science and Technology, 2016). Consequently, authorities have called for forensic analysts to rely solely on task-relevant information-and to actively avoid task-irrelevant information-when conducting analyses (National Commission on Forensic Science, 2015). In this study, we examined 97 evidence submission forms, used by 148 accredited crime laboratories across the United States, to determine what types of information laboratories solicit when performing latent print analyses. Results indicate that many laboratories request information with no direct relevance to the specific task of latent print comparison. More concerning, approximately one in six forms (16.5%) request information that appears to have a high potential for bias without any discernible relevance to latent print comparison. Solicitations for task-irrelevant information may carry meaningful consequences and current findings inform strategies to reduce the potential for cognitive bias.