ABSTRACT
A new antitumor antibiotic, fredericamycin A (FCRC-A48, NSC-305263), has been isolated from a strain of Streptomyces griseus (FCRC-48). Based on its unique ultraviolet-visible spectrum, infrared spectrum, proton and carbon-13 nuclear magnetic resonance spectra and mass spectra, it is judged to be a novel acid-base indicator type of compound. Its production, isolation and physicochemical properties are discussed. The isolation, ultraviolet-visible spectrum and some biological properties of two minor components, fredericamycin B and fredericamycin C, are also described.
Subject(s)
Antibiotics, Antineoplastic/isolation & purification , Antibiotics, Antineoplastic/biosynthesis , Chemical Phenomena , Chemistry, Physical , Chromatography, High Pressure Liquid , Chromatography, Thin Layer , Fermentation , Isoquinolines/isolation & purification , Spiro Compounds/isolation & purification , Streptomyces griseus/metabolismABSTRACT
Isolation of a pentaene macrolide antibiotic (NSC-277813) from Streptomyces griseus (FCRC-21) fermentation broth is described. Using field desorption mass spectrometry and high resolution field desorption mass spectrometry on the intact and derivatized antibiotic and degradation products, the antibiotic was identified as fungichromin. The application of field desorption mass spectrometry in the identification of polyene antibiotics is discussed.
Subject(s)
Antibiotics, Antineoplastic , Mass Spectrometry , Antibiotics, Antineoplastic/analysis , Antibiotics, Antineoplastic/isolation & purification , Macrolides , Methods , Polyenes/analysis , Polyenes/isolation & purification , Streptomyces griseus/analysisABSTRACT
Three antibiotics possessing cytotoxic properties were isolated from a strain of Streptomyces griseus (FCRC-57). One was found to be identical with griseorhodin A. A second, FCRC-57-U, was found to be identical to griseorhodin C. FCRC-57-G is a new antibiotic structurally related to griseorhodins A and C, and is active against KB cells in vitro. The structure of this new antibiotic was determined using mass spectrometry, proton and carbon nuclear magnetic resonance spectroscopy and synthesis.
