Temperature and photoperiod effects on dormancy status and life cycle parameters in Aedes albopictus and Aedes aegypti from subtropical Argentina.

Aedes albopictus (Diptera: Culicidae) distribution is bounded to a subtropical area in Argentina, while Aedes aegypti (Diptera: Culicidae) covers both temperate and subtropical regions. We assessed thermal and photoperiod conditions on dormancy status, development time and mortality for these species from subtropical Argentina. Short days (8 light : 16 dark) significantly increased larval development time for both species, an effect previously linked to diapause incidence. Aedes albopictus showed higher mortality than Ae. aegypti at 16 °C under long day treatments (16 light : 8 dark), which could indicate a lower tolerance to a sudden temperature decrease during the summer season. Aedes albopictus showed a slightly higher percentage of dormant eggs from females exposed to a short day, relative to previous research in Brazilian populations. Since we employed more hours of darkness, this could suggest a relationship between day-length and dormancy intensity. Interestingly, local Ae. aegypti presented dormancy similar to Ae. albopictus, in accordance with temperate populations. The minimum dormancy in Ae. albopictus would not be sufficient to extend its bounded distribution. We believe that these findings represent a novel contribution to current knowledge about the ecophysiology of Ae. albopictus and Ae. aegypti, two species with great epidemiological relevance in this subtropical region.

Thermal response in pre-imaginal biology of Ochlerotatus albifasciatus from two different climatic regions.

The biological processes on mosquito could be variable in response to local climatic characteristics. The thermal effects on time and the rate larval development, immature survival and adult size in local populations of Ochlerotatus albifasciatus (Macquart) (Diptera: Culicidae) from cold (Sarmiento) and temperate (Buenos Aires) regions from Argentina were evaluated. This species affects livestock production and human health. Larvae of both regions were placed in breeding thermal baths (11-32 °C range). Development and survival were recorded daily until adult emergence. The development temperature threshold and thermal constant for Sarmiento (4.59 ± 3.08 °C, 204.08 ± 7.83 degree days) was lower and higher than Buenos Aires, respectively (8.06 ± 1.81 °C, 149.25 ± 2.6 degree days). At cold temperatures (11-16 °C), Sarmiento larvae demonstrated 5 days faster development and higher survival (56%) than Buenos Aires (15%), whereas at warm temperatures (20-32 °C) were up to 2 days slower and similar survival (16% vs. 18%). The size did not show differences between populations. An Ochlerotatus albifasciatus population seems to present local thermal responses. The favourable temperature for survival and rate of development would vary within a cold or warm range, and these differential responses would explain the wide geographical distribution in different climatic regions of southern South America.

Liver Injury in Psoriasis Patients Receiving Ustekinumab: A Retrospective Study of 44 Patients Treated in the Clinical Practice Setting.

INTRODUCTION: The therapy of patients with psoriasis and liver disease can be a challenge due to the increased risk of adverse effects from traditional systemic treatments; in addition, although the anti-tumor necrosis factor agents are considered safer, they have also been associated with drug-induced liver injury and reactivation of viral hepatitis. Ustekinumab has a different mechanism of action and the little that is known of its effects on the liver comes from pivotal studies. The objectives of this study were to estimate the incidence of drug-induced liver injury in patients treated with ustekinumab in daily clinical practice and to analyze liver alterations in those patients with pre-existing liver disease. METHOD: All patients treated with the standard regimen of ustekinumab were included in the study. Variables gathered included age, sex, type of psoriasis, nail involvement, arthritis, previous treatments, history of liver disease, viral serology, Psoriasis Area Severity Index (at baseline and at 12, 16, and 52 weeks), transaminase levels, manifestations of liver disease, liver ultrasound, and factors such as body mass index, alcohol consumption, and ferritin levels. RESULTS: Grade 1 elevation of the transaminases was only observed in 6 patients; no cases of severe hypertransaminasemia were observed. None of the patients with elevation of the transaminases at baseline developed problems during treatment. CONCLUSIONS: Ustekinumab-related liver injury is uncommon and mild. From a hepatic point of view, the drug appears safe, even in patients with pre-existing liver disease and those who have developed altered liver function previously with other drugs.

Autoantibodies against TIF-1-γ and CADM-140 in Spanish patients with clinically amyopathic dermatomyositis (CADM): clinical significance and diagnostic utility.

BACKGROUND: Patients with clinically amyopathic dermatomyositis (CADM) appear to be at risk for developing cancer and interstitial lung diseases, but population data to confirm this hypothesis are limited. Moreover, CADM presents cutaneous and histological findings that may overlap with subacute cutaneous lupus erythematosus (SCLE). OBJECTIVES: To determine the association between myositis-specific autoantibodies, myositis-associated autoantibodies and CADM in Spanish patients. In addition, to study the usefulness of these autoantibodies in the differential diagnosis between CADM and SCLE. METHODS: Serum samples were tested for myositis-specific autoantibodies and myositis-associated autoantibodies through immunoprecipitation and other standardized methods. RESULTS: Anti-CADM-p140 and anti-p155 antibodies were the only myositis-specific autoantibodies found and were associated with interstitial lung diseases and cancer respectively. No myositis-associated autoantibodies were found in CADM. Moreover, clinical subsets and proportions seemed to differ from Asian cohorts, where anti-CADM-p140 is considered a CADM hallmark antibody and a risk factor for the development of interstitial lung disease. Interestingly, anti-SSA was highly associated with SCLE, whereas no myositis-specific autoantibodies were found in this entity. LIMITATIONS OF THE STUDY: Association between CADM and myositis-specific autoantibodies and differences between CADM and SCLE were tested on a relatively small cohort of patients. CONCLUSION: There is an association between cancer-associated myositis and interstitial lung diseases and their hallmark autoantibodies in our cohort. In addition, the combined determination of myositis-specific autoantibodies and SSA autoantibodies may help to accurately discriminate SCLE from CADM.

Lupus eritematoso subagudo inducido por terbinafina en un paciente con lupus eritematoso sistémico / Lupus erythematosus induced by terbinafine in a patient with systemic lupus erythematosus

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Environmental factors related to the abundance and activity of Ochlerotatus albifasciatus (Diptera: Culicidae) in an agricultural landscape of steppe arid climate.

Ochlerotatus albifasciatus (Macquart) is a flood water mosquito whose highest density has been found associated both with natural landscapes (prairies or grazing fields) in temperate and subtropical regions and with rainfall events. In the current study, we aimed to find out how the marked differences between environmental factors of agricultural landscape patches in a steppe arid region affect the relative abundance of this species. In wetland patches, the high activity of adults was closely associated with the flood irrigation system, suggesting that the agricultural activity contributes to the proliferation of this mosquito. The steppe patches would constitute an adverse environment reflected by the abrupt decrease in abundance. Multiple linear regression showed that some explanatory variables, such as wetland patches and moment of the day (midday), did not contribute significantly to the relative abundance variation. In contrast, temperature, wind, and cloud cover seemed to regulate the biting activity of females. Temperature affected the activity of mosquitoes in the steppe but seemed to have no effect in wetland patches, where the activity of mosquitoes was permanent and more stable against changes in temperature. In the steppe, which presents low levels of humidity, scarce vegetation, and greater wind exposure, the activity seemed to be unstable against small thermal variations. The variability of the relative abundance of Oc. albifasciatus in an agricultural landscape was widely explained by temperature in combination with the microenvironment type, wind speed, and cloud cover and indirectly by human activity.

Pioderma gangrenoso tras cirugía de cadera / Pyoderma gangrenosum following a hip surgical procedure

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Resistance to freezing temperatures in Aedes (Ochlerotatus) albifasciatus (Macquart) eggs (Diptera: Culicidae) from two different climatic regions of Argentina.

Aedes (Ochlerotatus) albifasciatus (Macquart) has the capacity to proliferate in different kinds of climates within its distribution range in South America. With the aim of studying local thermal adaptations of eggs, we exposed egg stocks from two climatically different localities: temperate humid pampa (Buenos Aires) and cold arid Patagonian (Sarmiento), to freezing conditions and then evaluated the effect on some features at this level. First, we thermally described the substrate where this species lays its eggs in the arid region. A typical thermal condition during winter was 10 h at -12° C. Second, we evaluated the effect of freezing on primary hatching (vs total hatching) and embryo survival. We also compared the proportion of embryonated eggs from both populations. The proportions of embryonated eggs were not different between localities, with averages of 78% and 83% in Sarmiento and Buenos Aires, respectively. Survival was equally successful after freezing in the two localities with an average range between 94-99%. Whether or not the eggs from Buenos Aires and Sarmiento were under freezing conditions, hatching was more than 98% after the first flooding. The results suggest that eggs of Ae. albifasciatus from Sarmiento and Buenos Aires have the same ability to survive at extreme temperatures (<0° C), showing a regional thermal adaptation rather than a local one.

Reliability of field-based fitness tests in youth.

The objective of this systematic review was to study the reliability of the existing field-based fitness tests intended for use with children and adolescents. The medical electronic databases MEDLINE, SCOPUS and SPORTS DISCUS were screened for papers published from January 1990 to December 2009. Each study was classified as high, low or very low quality according to the description of the participants, the time interval between measurements, the description of the results and the appropriateness of statistics. 3 levels of evidence were constructed according to the number of studies and the consistency of the findings. 32 studies were finally included in the present review. The reliability of tests assessing cardiorespiratory fitness (9 studies), musculoskeletal fitness (12 studies), motor fitness (3 studies), and body composition (10 studies) was investigated. Although some fitness components warrant further investigation, this review provides an evidence-based proposal for most reliable field-based fitness tests for use with children and adolescents: 20-m shuttle run test to measure cardiorespiratory fitness; handgrip strength and standing broad jump tests to measure musculoskeletal fitness; 4×10 m shuttle run test for motor fitness; and height, weight, BMI, skinfolds, circumferences and percentage body fat estimated from skinfold thickness to measure body composition.

Role of cardiorespiratory fitness on the association between physical activity and abdominal fat content in adolescents: the HELENA study.

We examined the role of cardiorespiratory fitness (CRF) on the association between physical activity (PA) and abdominal fat content in adolescents. Abdominal fat content was measured by waist circumference, waist-to-height ratio and dual energy X-ray absorptiometry (DXA) at 3 regions (R1, R2, and R3). PA and CRF were assessed by accelerometry and the 20 m-shuttle run test, respectively. Vigorous PA was inversely associated with waist circumference and waist-to-height ratio (ß from -0.149 to -0.197; p<0.05). In both CRF groups (i. e., low and high), vigorous and moderate-to-vigorous PA (also average PA in the low CRF group) were inversely associated with abdominal fat (i. e., R1, R2 and R3; ß from -0.146 to -0.244; p<0.05). This association did not differ according to CRF group (P for interaction >0.05), yet the percentage of variance in abdominal fat content, when assessed by DXA, explained by PA was slightly higher in adolescents with low CRF (3-8%) compared to those with high CRF (2-4%). The findings indicate that there is not a clear interaction effect of CRF in the association between PA and abdominal fat in adolescents, yet slightly stronger association was observed in the low fit group.

Assessing health-related fitness tests in the school setting: reliability, feasibility and safety; the ALPHA Study.

The aim of this study was to determine the reliability, feasibility and safety of a health-related fitness test battery administered by Physical Education (PE) teachers in the school setting. Six PE teachers, from three primary schools and three secondary schools, assessed twice (7 days apart) the 20 m shuttle run, handgrip and standing long jump tests, as well as weight, stature, triceps and subscapular thickness and waist circumference in 58 children (age: 6-11 yr) and 80 adolescents (age: 12-18 yr). Feasibility and safety were assessed by researches by means of direct observation. Significant inter-trial differences were found for the standing long jump test (3.8+/-12.7 cm, P<0.05) and for stature (0.73+/-0.8 cm, P<0.001) in children, and for waist circumference in both children and adolescents (-0.82+/-1.2 cm and -0.35+/-0.8 cm respectively, P=0.001). The feasibility and safety items assessed presented a successful answer. Therefore, the results indicate that health-related fitness tests administered by PE teachers are reliable, feasible and safe to be performed in the school setting.