ABSTRACT
The antioxidant properties of the wild growing sage species, Salvia reflexa Hornem., were investigated. The presence of superoxide (O(2)(.-)) and hydroxyl ((.)OH) radicals, malonyldialdehyde (MDA), reduced glutathione (GSH) and total flavonoids were observed in the above-ground parts of plant, as well as activities of the antioxidant enzymes superoxide-dismutase (SOD) and peroxidase (P-ase). The potential antioxidant activity of the methanol:water extract has been assessed based on scavenging activity of stable DPPH free radicals. By the means of TLC and LC/MS a screening for secondary plant products was performed. Significant quantities of O(2)(.-), (.)OH and MDA were observed. Thus, this species exhibited high SOD and P-ase activities as well as a content of total flavonoids. The dominant naturally occurring compound was rosmarinic acid. The results obtained suggested a high antioxidant activity of Salvia reflexa Hornem.
Subject(s)
Antioxidants/chemistry , Free Radical Scavengers/chemistry , Lamiaceae , Cinnamates/analysis , Depsides , Humans , Hydroxyl Radical/analysis , Malondialdehyde/analysis , Plant Extracts/chemistry , Reactive Oxygen Species , Superoxides/analysis , Rosmarinic AcidABSTRACT
The major pathway of metabolic transformation of N-deacetyl-N-formylcolchicine is the oxidative cytochrome P450 dependent O-demethylation of substituents in the aromatic ring A and tropolone ring C. It was found that O-demethylation (both, in vitro and in vivo) takes place predominantly in the aromatic ring, especially at position 2.
Subject(s)
Colchicine/analogs & derivatives , Colchicine/pharmacokinetics , Animals , Biotransformation , Dealkylation , In Vitro Techniques , Male , Microsomes, Liver/metabolism , Rats , Rats, WistarABSTRACT
Diallel crosses between five divergent vulgare wheat cultivars were made in order to evaluate the mode of inheritance and combining ability of grain proteins. Significant differences in grain protein content were found between cultivars and their hybrids. It was established that the inheritance of seed protein in the F1 generation included both additive and non-additive gene action.
Subject(s)
Cholelithiasis/metabolism , Trace Elements/metabolism , Female , Humans , Male , YugoslaviaABSTRACT
From the bulbs, flowers and flowerstalks of Colchicum visiani Parl. of Yugoslavian origin, were isolated and identified the neutral-phenolic and alkaline alkaloids listed in Table II. This investigation showed that the alkaloid content of Colchicum visiani Parl. is similar to that of Colchicum autumnale L., with the exception of a tropolonic alkaloid hR (f)-16 (found as well in Colchicum dörfleri) and a tropolonic alkaloid hR (f)-13, which had so far been unidentified in Colchicum species.