Meloidogyne incognita Survival in Soil Infested with Paecilomyces lilacinus and Verticillium chlamydosporium.

Meloidogyne incognita-infected tomato seedlings were transplanted into sterilized soil or unsterilized soil collected from 20 California tomato fields to measure suppression caused by Paecilomyces lilacinus, Verticillium chlamydosporium, and other naturally occurring antagonists. Unsterilized soils Q, A, and H contained 35, 39, and 55% fewer M. incognita second-stage juveniles (J2) than did sterilized soil 1 month after infected tomato seedlings were transplanted to these soils and placed in a greenhouse. Three months after infected seedlings were transplanted to unsterilized or sterilized soil, unsterilized soils K, L, and Q had 97, 62, and 86% fewer J2 than the corresponding sterilized soils. Unsterilized soils of M. incognita-infected seedlings that were maintained 1 month in a greenhouse followed by 1 or 2 months of post-harvest incubation contained J2 numbers equal to, or greater than, numbers in the corresponding sterilized soil. The most suppressive of the unsterilized soils, K and Q, were not infested with V. chlamydosporium. Paecilomyces lilacinus and V. chlamydosporium increased in colony forming units in unsterilized soil of all bioassays, but they were not associated with lower numbers of J2.

Overwintering Stages of Meloidogyne incognita in Vitis vinifera.

The overwintering of Meloidogyne incognita in and around Vitis vinifera cv. French Colombard roots was studied in a naturally infested vineyard at the Kearney Agricultural Center, in a growth chamber, in inoculated vines in microplots at the University of California, Davis, and in a greenhouse. Infected roots were sampled at intervals from onset of vine dormancy until plants accumulated about 800 degree days (DD - base 10 C). Embryogenesis within eggs, classified as less than or more than 16 cells and fully differentiated, and numbers of juveniles (second to fourth stage) and preovipositional and mature (egg-laying) adult stages in roots were determined. All stages were present at the onset of dormancy. Juveniles and immature females were not recovered during the dormant period. Mature females and eggs were always present in roots, although the number of mature females generally decreased with time after onset of dormancy. In contrast, in a greenhouse experiment that accumulated comparable DD without the host plant going through dormancy, the number of mature females increased. After bud break, the number of eggs per female increased and all nematode stages were found in host roots. Eggs in all stages of embryogenesis were observed at all times of sampling, indicating that females overwinter and are capable of laying eggs when conditions improve in the spring and need to be considered in nematode management decisions.

Density-dependent parasitism of the soil-borne nematodeCriconemella xenoplax by the nematophagous fungusHirsutella rhossiliensis.

Spatial sampling was used to investigate temporal density-dependent parasitism of the plant-parasitic nematodeCriconemella xenoplax byHirsutella rhossiliensis in three peach orchards on eight sample dates. The patches of soil in which the nematode and fungus interacted were assumed to possess similar density-dependent dynamics and to be small, independent, and asynchronous. Furthermore, sampling of separate patches was assumed to provide similar information with respect to density dependence as would temporal (repeated) sampling of the same patch. Percent parasitism was dependent on the number ofC. xenoplax/100 cm(3) soil (P=0.0001). The slope was unaffected by orchard or date but ranged from 0.0001 to 0.0043 depending on distance from the irrigation furrow. The relative shallowness of the slope and the large variation in percent parasitism not explained by nematode density suggest thatH. rhossiliensis is a weak regulator ofC. xenoplax population density.