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Fitoterapia ; 73(7-8): 583-96, 2002 Dec.
Article in English | MEDLINE | ID: mdl-12490216

ABSTRACT

A novel HPLC-UV method has been developed for the fingerprint analysis of the steroidal saponins in the rhizomes of three Ruscus species (Ruscus aculeatus, Ruscus hypoglossum and Ruscus colchicus). Saponins were identified by HPLC-ESI-MS. During the study a new major saponin was detected in the rhizomes of R. hypoglossum and R. colchicus. The structure of the new compound was defined as 1-O-[alpha-L-rhamnopyranosyl-(1-->2)-6-O-acetyl-beta-D-galactopyranosyl]-1beta,3beta,22xi,26-tetrahydroxy-furost-5(6)-en-26-O-beta-D-glucopyranoside (8) by spectral analysis.


Subject(s)
Ruscus/chemistry , Saponins/analysis , Saponins/chemistry , Chromatography, High Pressure Liquid , Mass Spectrometry , Molecular Structure , Plant Leaves/chemistry , Rhizome/chemistry , Ruscus/classification
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