ABSTRACT
Trichinella spp. from a sylvatic cycle has been found in several animal species such as pumas (Puma concolor), armadillos (Chaetophractus villosus), rats (Rattus norvegicus), and wild boars (Sus scrofa) in Argentina. Moreover, Trichinella infection has been detected in a wide range of marine mammals around the world, including polar bears (Ursus maritimus) and walruses (Odobenus rosmarus). Until the present time, Trichinella spp. infection has not been detected in marine mammals of South America. Samples from four South American sea lions (Otaria flavescens) found dead in Rio Negro, Argentina, were analyzed by artificial digestion, and in the case of one animal, Trichinella larvae were identified at the species level by nested multiplex PCR as Trichinella spiralis. This is the first report of a Trichinella species infecting marine mammals from South America.
Subject(s)
Muscle, Skeletal/parasitology , Sea Lions/parasitology , Trichinella spiralis/isolation & purification , Trichinellosis/veterinary , Animals , Argentina , Larva , Puma/parasitology , Rats , South America , Sus scrofa/parasitology , Swine , Trichinellosis/parasitology , Ursidae/parasitology , Walruses/parasitologyABSTRACT
Trichinella spiralis has been documented in wild animals in Argentina, including puma, armadillos, rats and wild boars. In 2008, molecular analysis identified Trichinella T12 from a naturally infected puma (Puma concolor) from Patagonia. The aim of the present work was to study the relationship between the infectivity and pathology of Trichinella T12 in the puma and in domestic cats, and the possible risks that may be present for transmission among these animals. Two cats (A and B) were orally-infected with 3300 and 1850 Trichinella T12 muscle larvae, respectively; one additional cat was used as a control. During the 54 days post-infection, a daily examination was performed which included monitoring body temperature, and cardiac and respiration rates; the animals were then euthanized. Hematological studies included hematocrit (%), hemoglobin (g/dl), and white cell, neutrophil, lymphocyte and eosinophil counts. Blood biochemistry included urea, creatinine, AST, ALT, CK, LDH and ALP. An ELISA assay was also performed. At necropsy, organs (liver, spleen, brain, cerebellum and kidney), nails and muscle samples were obtained for histopathology studies and artificial digestion. The muscles that were studied included the diaphragm, massetter, cutaneous, temporal, intercostals, lumbar, tongue, limbs, neck and tail. Clinical signs, such as anorexia, diarrhea, vomiting, shaggy hair, decay and muscle pain, were observed in both cats. The eosinophil counts were elevated in both cats A and B. Trichinella larvae were recovered from all of the muscles analyzed where the histopathology showed larvae in several muscles without degenerative reaction. Neither larvae nor lesions were observed in non-muscular organs. Cat A had a maximum of 246 larvae per gram (lpg) in the temporal muscle and a minimum of 80 lpg in the tongue, while cat B had a maximum of 65 lpg in muscles of the leg and a minimum of 10 lpg in tail muscles. This study represents the first record of experimental infection of cats with Trichinella T12.
Subject(s)
Antibodies, Helminth/blood , Cat Diseases/parasitology , Puma/parasitology , Trichinella spiralis/immunology , Trichinellosis/veterinary , Animals , Argentina , Blood Chemical Analysis , Cat Diseases/immunology , Cat Diseases/pathology , Cat Diseases/transmission , Cats , Enzyme-Linked Immunosorbent Assay , Larva , Muscles/parasitology , Trichinellosis/immunology , Trichinellosis/parasitology , Trichinellosis/pathologyABSTRACT
Patient. We report a 51-year-old male presenting with Grade III rhabdomyosarcoma.Discussion. A case of rhabdomyosarcoma which developed in proximity to a metal surgical implant is described. Few cases have been reported in the world in humans.The therapeutic approach to the disease is presented, together with a brief review of literature.
