ABSTRACT
BACKGROUND: MicroRNAs (miRNAs) are non-coding small RNAs, involved in post-transcriptional regulation of many target genes. METHODS: Five miRNAs that have been consistently found deregulated in PCa (miR-21, miR-141, miR-214, miR-375, and let-7c) were analyzed in urinary pellets from 60 prostate cancer (PCa) patients and 10 healthy subjects by qRT-PCR. Besides, urinary exosomes were isolated by differential centrifugation and analyzed for those miRNAs. RESULTS: Significant upregulation of miR-21, miR-141, and miR-375 was found comparing PCa patients with healthy subjects in urinary pellets, while miR-214 was found significantly downregulated. Regarding urinary exosomes, miR-21 and miR-375 were also significantly upregulated in PCa but no differences were found for miR-141. Significant differences were found for let-7c in PCa in urinary exosomes while no differences were observed in urinary pellets. A panel combining miR-21 and miR-375 is suggested as the best combination to distinguish PCa patients and healthy subjects, with an AUC of 0.872. Furthermore, the association of miRNAs with clinicopathological characteristics was investigated. MiR-141 resulted significantly correlated with Gleason score in urinary pellets and let-7c with clinical stage in urinary exosomes. Additionally, miR-21, miR-141, and miR-214 were found significantly deregulated in intermediate/high-risk PCa versus low-risk/healthy subjects in urinary pellets. Significant differences between both groups were found in urinary exosomes for miR-21, miR-375, and let-7c. CONCLUSIONS: These findings suggest that the analysis of miRNAs-especially miRNA-21 and miR-375- in urine could be useful as biomarkers in PCa. Prostate 77: 573-583, 2017. © 2016 Wiley Periodicals, Inc.
Subject(s)
Biomarkers, Tumor/urine , Exosomes/metabolism , MicroRNAs/urine , Prostatic Neoplasms/diagnosis , Prostatic Neoplasms/urine , Aged , Aged, 80 and over , Biomarkers, Tumor/genetics , Exosomes/genetics , Humans , Male , MicroRNAs/genetics , Middle Aged , Prognosis , Prostatic Neoplasms/geneticsABSTRACT
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Subject(s)
Practice Patterns, Physicians' , Clinical Competence , Process Assessment, Health Care , Consensus Development Conferences as TopicABSTRACT
INTRODUCTION: A spectacular increase was noticed in the number of consultations for insect bites at the Sant Cugat Primary Care Center (Barcelona, Spain). Subsequent study of the species in the area identified Aedes albopictus. OBJECTIVE: To analyze consultations for insect bites in this center (1998-2004). METHODS: A descriptive, cross sectional study with a 7-year retrospective review was performed. A total of 2,760 patients consulted. RESULTS: A continual increase in the number of consultations for insect bites was detected, reaching 16 consultations per 1,000 inhabitants. The mean age of the patients was 32 years and 62% were women. Seventy-one percent of the bites were located on the extremities and 19% were infected. Sixty-seven percent of the patients received systemic treatment. CONCLUSIONS: Primary care alerted the town council of the significant increase in consultations for insect bites. Patients consulting for this reason were predominantly young women who frequently received systemic treatment and showed a high percentage of local complications.
Subject(s)
Aedes , Insect Bites and Stings/epidemiology , Adult , Animals , Cross-Sectional Studies , Female , Humans , Insect Bites and Stings/complications , Insect Vectors , Male , Public Health , Retrospective Studies , Spain/epidemiology , Species Specificity , Wound Infection/epidemiologyABSTRACT
Introducción: En el Centro de Atención Primaria de Sant Cugat del Vallès (Barcelona) se detectó un espectacular aumento de consultas por picaduras de insectos. El posterior estudio de las especies del área permitió identificar a Aedes albopictus. Objetivo: Analizar las consultas por picaduras de insecto (1998-2004). Métodos: Estudio descriptivo transversal retrospectivo. Consultaron 2.760 pacientes durante el período 1998-2004. Resultados: Se detectó un continuo aumento de consultas por picaduras, hasta alcanzar las 16 consultas por 1.000 habitantes. Los pacientes tenían una media de edad de 32 años, y un 62% eran mujeres. El 71% de las lesiones se localizaron en las extremidades y se infectaron el 19% de ellas. El 67% de los pacientes recibió tratamiento sistémico. Conclusiones: La atención primaria alertó sobre el llamativo aumento de consultas por picaduras de insecto. El colectivo de pacientes que consultó por este motivo fue mayoritariamente femenino, joven, recibió con frecuencia tratamiento sistémico y presentó un elevado porcentaje de complicaciones locales
Introduction: A spectacular increase was noticed in the number of consultations for insect stings at the Sant Cugat Primary Care Center (Barcelona, Spain). Subsequent study of the species in the area identified Aedes albopictus. Objective: To analyze consultations for insect stings in this center (1998-2004). Methods: A descriptive, cross sectional study with a 7-year retrospective review was performed. A total of 2,760 patients consulted. Results: A continual increase in the number of consultations for insect stings was detected, reaching 16 consultations per 1,000 inhabitants. The mean age of the patients was 32 years and 62% were women. Seventy-one percent of the stings were located on the extremities and 19% were infected. Sixty-seven percent of the patients received systemic treatment. Conclusions: Primary care alerted the town council of the significant increase in consultations for insect stings. Patients consulting for this reason were predominantly young women who frequently received systemic treatment and showed a high percentage of local complications
