[Research on immune system response, skin and hepatic reactivity in laboratory animals after retro-auricular topical administration of two reactive textile dyes]. / Testarea pe animale de laborator a raspunsului sistemului imunitar, a reactivitatii cutanate si hepatice, dupa administrarea topica retroauriculara a doi coloranti reactivi textili.

AIM: To evaluate immune system response, skin and hepatic reactivity in Swiss mice after retro auricular local administration of two textile dyes. MATERIAL AND METHODS: On 3 groups of white Swiss mice: group I (Cibacron Yellow F-4G), group II (Cibacron Orange P-2R) and control group (DMSO dimethylsulfoxide), substances were applied on left retro auricular ear, in dose of 25 microL solution 10% in DMSO (the dyes were not soluble in water), once a day, for 7 days. After euthanasia, blood samples were taken to assay differential cell count, peripheral neutrophils activity (NBT test), serum opsonic capacity, peritoneal macrophages activity (phagocytic and bactericidal capacity), and activity of spleenic T-lymphocytes with rossetting capacity, and spleen cells forming Jerne plaques. There were taken skin from local application of dyes, retro auricular and later cervical nodes from left side and hepatic samples. RESULTS: Retro auricular and later cervical nodes mass increases after administration of textile dyes, compared to control group. Basophiles percentages were increased after administration of two dyes (stronger after yellow dye). Peripheral neutrophils activity was increased after administration of two dyes, especially after orange dye. Administration of the two dyes decreased serum opsonic capacity and peritoneal macrophages activity, but did not influenced the activity of spleenic T-lymphocytes with rossetting capacity and spleen cells forming Jerne plaques. There were significant skin and hepatic changes in microscopy. CONCLUSION: The studied dyes influenced the activity of immune system, increased skin and hepatic reactivity.

[Experimental study of immune reactivity in Swiss mice due to topical administration of three textile dyes]. / Studiu experimental pe soareci swiss privind modul de reactie a sistemului imunitar în urma administrarii topice a trei coloranti reactivi textili.

UNLABELLED: Reactive Red 183, Reactive Red 2 and Reactive Blue 204 (red dye, green dye and blue dye) are three reactive dyes frequently used in textile industry. In some atmospheric conditions ( high temperature, perspiration, pH values, UV/IR radiations), some quantities of these hydrolyzed dyes, could pass from textile clothes directly into the human skin. MATERIAL AND METHOD: There were used 4 groups of white Swiss mice (with similar weight and number of both sexes), control group and 3 groups, treated once daily with a retro-auricular application of different reactive dyes. After 14 days of treatment, blood samples were taken from retro-orbitary plexus to assess leukocyte count, phagocytic capacity of peripheral neutrophils, serum opsonic capacity, phagocyte capacity and bactericidal capacity of peritoneal macrophages, splenic T lymphocytes with rossetting capacity and spleen cells forming Jerne plaques. The retro-acuricular and latero-cervical nodes were weighted. RESULTS: Red dye did not influence the weight of the studied nodes, but determined statistically significant modifications on non-specific immune system parameters. Blue and grena dyes determined modifications of weight especially of retroauricular nodes. Grena dye determined important effects of non-specific immune system parameters (serum opsonic capacity, phagocyte capacity and bactericidal capacity of peritoneal macrophages). The blue dye did not determine a biological response. CONCLUSION: Red and green dye determined important effects on non-specific immune system parameters.

[Dietary survey in an obese population]. / Ancheta alimentara într-o populatie obeza.

The body mass index (BMI) shows a rate of 17.4% patients suffering from first degree fatness and 56.5% from second degree fatness. The frequency of the illness increases with age, the differences established being statistically important for a p of 0.001. 45.6% persons were diagnosed with HTA, with differences statistically important at a p of 0.01, so the number of cases increases with age. The same situation is to be found in cardiovascular illnesses (angina pectoris, ischaemic cardiopathy). The high level of cholesterol is more frequent at older people, and still the differences established are statistically not significant. In these conditions, the diet must be adapted to the patient's condition. A comparative study was made on groups of age, using the following groups of food: cereal derivatives, vegetables, fruits, dairy product, meat, fish. In most of the cases, the differences established are statistically not significant. So, even if the diagnosis is known, the patient doesn't give up his eating habits.