Was there a significant difference in sleep shifts in the high school population due to the COVID-19 pandemic depending on chronotype? A nationwide cross-sectional study.

The aim of this study was to detect whether the COVID-19 pandemic has caused changes in the sleep cycle (subjective sleep shifts) of high school students divided into a sample of young women - W (n = 1999, age = 17.65 ± 2.39 y) and young men - M (n = 1094, age = 17.49 ± 1.74 y) in Slovakia depending on circadian preference in comparison with the term before COVID-19. The present cross-sectional study employed a self-reported standardized questionnaire (Morningness-Eveningness Questionnaire) to study circadian preference, which was complemented by a question focused on subjective sleep shifts before and during the pandemic. The results revealed significant strong dependence between circadian preference and subjective sleep shift in both W (χ2(8) = 153.1, p < .01, Cramer's V = .20, p < .01) and M (χ2(8) = 98.3, p < .01, Cramer's V =.21, p < .01). The delay of the sleep cycle has mainly become apparent in the case of definite evening types (W: 75.7%; M: 71.8%) and moderate evening types (W: 83.1%; M: 70.3%). The delay also prevailed in the intermediate types (W: 61.9%; M: 53.8%). Subjective sleep shifts were not confirmed (W: 93.8%; M: 35.3%) in the definite morning type. The sleep cycle was changed to earlier hours of definite morning types (W: 6.3%; M: 52.9%). It is necessary to focus on definite and moderate evening types and regulate the unsuitable state to time shift of the sleep cycle.

Modulation of cough response by sensory inputs from the nose - role of trigeminal TRPA1 versus TRPM8 channels.

BACKGROUND: Cough, the most important airways defensive mechanism is modulated by many afferent inputs either from respiratory tussigenic areas, but also by afferent drive from other organs. In animal models, modulation of cough by nasal afferent inputs can either facilitate or inhibit the cough response, depending on the type of trigeminal afferents stimulated. METHODS: In this study we addressed the question of possible bidirectional modulation of cough response in human healthy volunteers by nasal challenges with TRPA1 and TRPM8 agonists respectively. After nasal challenges with isocyanate (AITC), cinnamaldehyde, (-) menthol and (+) menthol (all 10-3 M) nasal symptom score, cough threshold (C2), urge to cough (Cu) and cumulative cough response were measured). RESULTS: Nasal challenges with TRPA1 relevant agonists induced considerable nasal symptoms, significantly enhanced urge to cough (p<0.05) but no statistically significant modulation of the C2 and cumulative cough response. In contrast, both TRPM8 agonists administered to the nose significantly modulated all parameters including C2 (p<0.05), Cu (p<0.01) and cumulative cough response (p <0.01) documenting strong anti irritating potential of menthol isomers. CONCLUSIONS: In addition to trigeminal afferents expressing TRP channels, olfactory nerve endings, trigemino - olfactoric relationships, the smell perception process and other supramedullar influences should be considered as potential modulators of the cough response in humans.