[An immunoglobulin preparation as a calibrating agent for the gel filtration method]. / Preparat immunoglobulina v kachestve kalibranta dlia metoda gel-fil'tratsii.

The fractional composition of commercial immunoglobulin was studied by the method of gel filtration on ultragel AcA-34 and the possibility of its use for the calibration of a chromatographic column was shown. The fractionation of a specimen of IgG revealed the presence of 4 fractions. Their molecular weights corresponded to dimers, monomers, Fab fragments and low-molecular peptides. The qualitative fractional composition of the preparation was shown to be stable during 3 years of storage, as well as after keeping the preparation at 37 degrees C for 1 month. The attested characteristic of each IgG fraction, the distribution coefficient (Kav), was established. The use of a specimen of immunoglobulin obtained with the use of the modified Cohn's method--as calibrant will make it possible to calibrate the columns for a shorter period and to control the correctness of the analysis.

[A gel filtration study of the fractional composition of tetanus antitoxic sera]. / Izuchenie fraktsionnogo sostava stolbniachnykh antitoksicheskikh syvorotok metodom gel'-fil'tratsii.

The fraction composition of tetanus antitoxic sera has been studied by the method of gel filtration in columns packed with Sephadex G-200. Differences in the fraction composition of preparations manufactured by different enterprises have been revealed. The specific activity of antitoxic sera has been found to depend on the degree of their fragmentation.

[Molecular composition of the fractions of immunoglobulin preparations studied by gel filtration]. / Izuchenie molekuliarnogo sostava fraktsii immunoglobulinovykh preparatov metodom gel'-fil'tratsii.

The method of gel filtration in columns permitted the separation of aggregated fractions into polymers whose content did not exceed 10% and dimers, their content ranging from 3.6% to 22.11%. The preparations were also found to contain fractions of monomers and fragments, Fab-fragments being detected in 10 out of 20 batches under study (4.04-27.36%). The shelf life of all preparations did not exceed 8-12 months. The use of spectrophotometric techniques ensured obtaining the most objective results in the calculation of the percentage of fractions contained in immunoglobulin preparations. The evaluation of the molecular composition of immunoglobulin preparations by the method of gel filtration is conducive to the improvement of their quality.

[Dependence of the specific activity of an immunoglobulin against tick-borne encephalitis on the degree of its fragmentation]. / Izuchenie zavisimosti spetsificheskoi aktivnosti immunoglobulina protiv kleshchevogo éntsefalita ot stepeni ego fragmentatsii.

Immunoglobulin preparations against tick-borne encephalitis with the increasing content of Fab-fragments (from 16% to 45%) have been experimentally obtained. As revealed by testing these samples in vivo for specific activity (in the biological neutralization test), the preparations containing 16% of Fab-fragments show no perceptible decrease of specific activity; its sharp decrease (2-16 times) can be observed in preparations with a high degree of fragmentation (the content of Fab-fragments being equal to 30-45%).

[Dependence of the specific activity of antistaphylococcal immunoglobulin on the degree of its fragmentation]. / Izuchenie zavisimosti spetsificheskoi aktivnosti antistafilokokkovogo immunoglobulina ot stepeni fragmentatsii.

In vitro experiments have revealed no perceptible decrease in the content of antitoxic antibodies to alphastaphylolysin in the preparations of antistaphylococcal immunoglobulin, containing 13-50% of Fab-fragments. These data confirm the necessity of controlling the molecular composition of the preparations of antistaphylococcal IgG by physico-chemical methods with the aim to evaluate their quality, as the fragmented preparations are rapidly eliminated from a human body.

[Dependence of the specific activity of antitetanus immunoglobulin on the degree of its fragmentation]. / Izuchenie zavisimosti spetsificheskoi aktivnosti protivostolbniachnogo immunoglobulina ot stepeni fragmentatsii.

Antitetanus immunoglobulin preparations with the increasing content of Fab-fragments (15, 30, 53%) have been obtained under specific experimental conditions. Tests for specific activity have revealed an insignificant decrease (13%) in this activity in the preparation containing 15% of Fab-fragments and its sharp drop in the preparations containing 30-50% of Fab-fragments. The specific activity of antitetanus immunoglobulin has been found to be related to the degree of its fragmentation.

[Ratio of admixtures of blood group substances and heme pigments in immunoglobulin preparations from different types of raw material]. / Sootnoshenie primesei gruppovykh veshchestv krovi i gempigmentov v preparatakh immunoglobulinov iz razlichnykh vidov syr'ia.

The study of 192 batches of immunoglobulin preparations for the presence of the admixtures of blood group substances and blood pigments has revealed that preparations obtained from abortion and placental blood sera significantly differ from donor immunoglobulins by a greater content of the above admixtures. The absence of correlation between the content of blood pigments and blood group substances in preparations from abortion and placental blood has been significantly demonstrated.