Preparation of cyclic 2',3'-monophosphates of oligoadenylates (A2'p)nA > p and A3'p(A2'p)n-1A > p.

The action of the guanylyl-preferring RNase from Bacillus intermedius (binase) on a mixture of oligoadenylates with randomly distributed 2'-5' and 3'-5' internucleotide bonds [(A2'/3'p)n] under conditions sufficient for complete hydrolysis of poly(A) results in a mixture containing a single circular oligoadenylate and two series of linear oligoadenylates ending in cyclic 2',3'-phosphate. Individual compounds formed upon digestion of (A2'/3'p)n with binase have been isolated. Their structure was determined on the basis of their chemical and enzymatic conversions and confirmed by 1H-, 13C- and 31P-NMR spectra. According to these data, the circular triadenylate contains one 2'-5' and two 3'-5' internucleotide bonds, linear oligoadenylates of one series contain exclusively 2'-5' internucleotide bonds [(A2'p)nA > p], while each compound of the other series contains a single 3'-5' internucleotide bond connecting the 5'-ultimate nucleotide residue with the penultimate one [A3'p(A2'p)n-1A > p]. The incubation of compounds of the former series A3' p(A2'p)n > p at pH 1.0 and the subsequent action of phosphatase results in successive formation of compounds of two other new series: A3'p(A2'p)nA2'(3')p and A3'p(A2'p)nA.

[Isolation of influenza virus from Chlidonias nigra and serologic examination of the birds for antibodies to influenza virus]. / Izoliatsiia virusa grippa ot chernoi krachki (Chlidonias nigra) i serologicheskoe obsledovanie ptits na antitela k virusu grippa.

Influenza A virus with the antigenic formulae Hav4Neq2 has been isolated from Chlidonias nigra in the region of mass moulting in the territory of the Kazakh SSR. Antihemagglutinins for the newly isolated virus were detected in the sera of some specimens of the sea gull order. The data obtained suggest an active circulation of the virus in this region during the summer of 1977.