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1.
Metallomics ; 14(10)2022 10 20.
Article in English | MEDLINE | ID: mdl-36208212

ABSTRACT

Understanding the nanoscale chemical speciation of heterogeneous systems in their native environment is critical for several disciplines such as life and environmental sciences, biogeochemistry, and materials science. Synchrotron-based X-ray spectromicroscopy tools are widely used to understand the chemistry and morphology of complex material systems owing to their high penetration depth and sensitivity. The multidimensional (4D+) structure of spectromicroscopy data poses visualization and data-reduction challenges. This paper reports the strategies for the visualization and analysis of spectromicroscopy data. We created a new graphical user interface and data analysis platform named XMIDAS (X-ray multimodal image data analysis software) to visualize spectromicroscopy data from both image and spectrum representations. The interactive data analysis toolkit combined conventional analysis methods with well-established machine learning classification algorithms (e.g. nonnegative matrix factorization) for data reduction. The data visualization and analysis methodologies were then defined and optimized using a model particle aggregate with known chemical composition. Nanoprobe-based X-ray fluorescence (nano-XRF) and X-ray absorption near edge structure (nano-XANES) spectromicroscopy techniques were used to probe elemental and chemical state information of the aggregate sample. We illustrated the complete chemical speciation methodology of the model particle by using XMIDAS. Next, we demonstrated the application of this approach in detecting and characterizing nanoparticles associated with alveolar macrophages. Our multimodal approach combining nano-XRF, nano-XANES, and differential phase-contrast imaging efficiently visualizes the chemistry of localized nanostructure with the morphology. We believe that the optimized data-reduction strategies and tool development will facilitate the analysis of complex biological and environmental samples using X-ray spectromicroscopy techniques.


Subject(s)
Nanoparticles , Nanostructures , X-Rays , Software , Algorithms
2.
Front Neurosci ; 12: 195, 2018.
Article in English | MEDLINE | ID: mdl-29643761

ABSTRACT

We have calculated key characteristics of associative (content-addressable) spatial-temporal memories based on neuromorphic networks with restricted connectivity-"CrossNets." Such networks may be naturally implemented in nanoelectronic hardware using hybrid memristive circuits, which may feature extremely high energy efficiency, approaching that of biological cortical circuits, at much higher operation speed. Our numerical simulations, in some cases confirmed by analytical calculations, show that the characteristics depend substantially on the method of information recording into the memory. Of the four methods we have explored, two methods look especially promising-one based on the quadratic programming, and the other one being a specific discrete version of the gradient descent. The latter method provides a slightly lower memory capacity (at the same fidelity) then the former one, but it allows local recording, which may be more readily implemented in nanoelectronic hardware. Most importantly, at the synchronous retrieval, both methods provide a capacity higher than that of the well-known Ternary Content-Addressable Memories with the same number of nonvolatile memory cells (e.g., memristors), though the input noise immunity of the CrossNet memories is lower.

3.
Ambio ; 42(2): 146-59, 2013 Mar.
Article in English | MEDLINE | ID: mdl-23475652

ABSTRACT

Barriers and bridges to implement policies about sustainable development and sustainability commonly depend on the past development of social-ecological systems. Production of metals required integration of use of ore, streams for energy, and wood for bioenergy and construction, as well as of multiple societal actors. Focusing on the Swedish Bergslagen region as a case study we (1) describe the phases of natural resource use triggered by metallurgy, (2) the location and spatial extent of 22 definitions of Bergslagen divided into four zones as a proxy of cumulative pressure on landscapes, and (3) analyze the consequences for natural capital and society. We found clear gradients in industrial activity, stream alteration, and amount of natural forest from the core to the periphery of Bergslagen. Additionally, the legacy of top-down governance is linked to today's poorly diversified business sector and thus municipal vulnerability. Comparing the Bergslagen case study with other similar regions in Russia and Germany, we discuss the usefulness of multiple case studies.


Subject(s)
Conservation of Natural Resources , Metallurgy/history , Cities , Forestry , Geography , History, 16th Century , History, 17th Century , History, 18th Century , History, 19th Century , History, 20th Century , Sweden , Water Supply
4.
Biosens Bioelectron ; 39(1): 152-5, 2013 Jan 15.
Article in English | MEDLINE | ID: mdl-22857907

ABSTRACT

Single photon counting is the most sensitive and accurate method for detection of very weak fluorescent signals obtained in many applications such as DNA sequencing, detection of biological reporters on micro-beads, detection of droplets in micro-fluidic systems, etc. In this paper we describe the use of single photon spectrometer for detection and characterization of very weak multicolor fluorescence produced by mixtures of various fluorescent dyes and quantum dots.


Subject(s)
Fluorescent Dyes/analysis , Quantum Dots , DNA/analysis , Photons , Spectrometry, Fluorescence
5.
Electrophoresis ; 28(21): 3890-900, 2007 Nov.
Article in English | MEDLINE | ID: mdl-17922519

ABSTRACT

We propose a novel method for electrokinetic injection of DNA samples into capillaries from nanoliter gel micropads, deposited on glass slides, which are coated with electroconducting film. Theoretical and experimental proof is presented for the proposed method. The method allows efficient and highly precise injection without physical contact between the gel pad and the capillary. Read length of more than 700 bp at Q20 has been reproducibly demonstrated in fused-silica capillaries using the proposed injection technique. Based on the obtained results we discuss a novel DNA sequencing system which combines DNA amplification and cycle sequencing in arrays of subnanoliter gel micropads and high-throughput electrophoretic separation in monolith multicapillary arrays.


Subject(s)
DNA/isolation & purification , Electrophoresis, Microchip/methods , Models, Chemical , Nanotechnology/methods , Polymerase Chain Reaction/methods , Sequence Analysis, DNA/methods , Base Sequence , Electrophoresis, Microchip/instrumentation , Equipment Design , Gels/chemistry , Indicators and Reagents , Polymerase Chain Reaction/instrumentation , Reproducibility of Results , Sensitivity and Specificity , Sequence Analysis, DNA/instrumentation , Spectrophotometry, Ultraviolet
6.
Electrophoresis ; 28(3): 317-21, 2007 Feb.
Article in English | MEDLINE | ID: mdl-17154326

ABSTRACT

A novel, nondamaging method for experimental characterization of the formation and propagation of high-resistivity zones in CE, based on the measurement of time-dependent Joule heating on the outer capillary surface is proposed. The method detects propagation of resistive regions in capillaries in real time and allows the estimation of their velocity and resistance. The presented experimental data are in agreement with the results of the computer simulation as well as with previous data on the subject. The proposed method is useful for the development of new polymers as well as for the refinement and optimization of new CE protocols.


Subject(s)
Computer Simulation , Temperature , Buffers , Electric Conductivity , Electrophoresis, Capillary , Sequence Analysis, DNA/instrumentation , Sequence Analysis, DNA/methods
7.
Electrophoresis ; 26(18): 3430-7, 2005 Sep.
Article in English | MEDLINE | ID: mdl-16167363

ABSTRACT

A novel design of the detection zone in multicapillary arrays used for electrophoretic separation is presented. The use of a detection gap (DG), in which the reflective surfaces separating the channels of the array are eliminated, is proposed to improve the illumination and detection of the separated DNA fragments. The electric field compression in the DG is achieved by optimization of the gap geometry. The results of the computer simulation and experiment demonstrate no substantial band-broadening in the DG. We believe that the proposed method will be useful for application in the microfabricated devices.


Subject(s)
Electrophoresis, Capillary/instrumentation , Sequence Analysis, DNA/methods , Computer Simulation , Electrophoresis, Capillary/methods , Fluorescence
8.
Electrophoresis ; 24(7-8): 1176-83, 2003 Apr.
Article in English | MEDLINE | ID: mdl-12707909

ABSTRACT

We have studied the formation of a resistive region in the capillary during DNA separation. This effect is caused by an unequal change in the mobilities of cations and anions at the interface between the running buffer solution and the capillary. We studied the motion of the resistive region boundary by sequential removal of portions of the affected capillary end. We found that in the process of developing the resistive region the distribution of the electric fields in the capillary changes from uniform to extremely nonuniform, with a very high field (above 1 MV/cm) in the resistive region and a reduced field (80 V/cm) in the rest of the capillary. Though theoretically a resistive region may appear either at the anode (detection) or the cathode (injection) end of the capillary, all previous publications report the formation of the resistive region at the cathode side. In our experiments, however, the anomalous region is formed at the anode. Thus, the separated DNA peaks move towards the slowly progressing resistive region. Our results indicate that the DNA is stopped at the boundary and does not enter the region. When the resistive region is clipped off the peak motion resumes. This suggests that there exists a potential barrier at the resistive layer boundary that prevents the drift of the peaks towards the anode. The formation of the resistive region interferes with a normal separation process causing a gradual decrease of the capillary current and the deceleration and eventual quenching of the peak motion. For the ABI chemistry, we experimented with adding polymers to the electrode buffer to equate the transference numbers for anions and cations, and found the conditions at which this effect is completely eliminated.


Subject(s)
DNA/isolation & purification , Electrophoresis, Capillary/standards , Buffers , Electric Impedance , Electrophoresis, Capillary/methods , Equipment Failure Analysis , Polymers
9.
Electrophoresis ; 24(7-8): 1184-92, 2003 Apr.
Article in English | MEDLINE | ID: mdl-12707910

ABSTRACT

Recently, we developed a family of high-performance automated capillary DNA sequencing instruments based on a single-photon detection of fluorescently labeled DNA fragments. Our machines employ digital and broadband techniques, essential for achieving superior instrument sensitivity and dynamic range. In the present paper, we discuss limitations of the instrument's performance caused by the nonlinearity of single-photon detectors as well as methods for nonlinearity compensation which increase the detection dynamic range and base-calling accuracy.


Subject(s)
Electrophoresis, Capillary/instrumentation , Nucleotides/analysis , Sequence Analysis, DNA/instrumentation , Electronics , Electrophoresis, Capillary/standards , Equipment Design , Fluorescent Dyes/analysis , Photons , Reference Values , Reproducibility of Results , Sequence Analysis, DNA/standards
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