ABSTRACT
The localization of enhanced green fluorescent protein (EGFP) was studied in preimplantation embryos obtained from reciprocal mating of hemizygous C57Bl/6-Tgn (ACTbEGFP)1Osb/J mice with C57Bl/6 mice. Specific fluorescence of EGFP was observed in all oocytes and embryos obtained from transgenic females during all preimplantation stages and in embryos inheriting the EGFP gene from transgenic males starting from the 8 blastomere stage during the compactization period. EGFP mRNA or EGFP synthesized during oogenesis can be retained in embryos during the entire preimplantation period, while expression of EGFP gene transferred from the father coincides with the onset of compactization. The possibility of using these embryos in experimental mammalian embryology is discussed.
Subject(s)
Blastocyst/physiology , Green Fluorescent Proteins/metabolism , Animals , Blastocyst/cytology , Female , Green Fluorescent Proteins/genetics , Male , Mice , Mice, Inbred C57BL , Oocytes/cytology , Oocytes/metabolismABSTRACT
Experimental studies of composite materials formed on the basis of fluorine-containing latex and bioactive polysaccharides showed that physicochemical properties of composite materials and their adhesion characteristics can be modulated by variations of polysaccharide-latex ratio and the nature of polysaccharides. The ratio of components ensuring the formation of biosynthetic films that meet the standards for modern wound coating and maintain adhesion and growth of substrate-dependent mammalian cells was determined. These materials can considerably increase the efficiency of treatment of extensive and deep skin wounds in cases when application of cell cultures is indicated.
Subject(s)
Bandages , Biocompatible Materials/chemistry , Cell Culture Techniques/methods , Wounds and Injuries , Cell Adhesion , Humans , Latex/chemistry , Materials Testing , Methylcellulose/chemistry , Polysaccharides/chemistryABSTRACT
We propose a method of creation of a 3D matrix consisting of native collagen fibers and natural polysaccharide chitosan. The collagen-chitosan hydrogels maintain viability and prolipherative activity of embryonic stem cells obtained from internal cells of mouse blastocyst. The proposed system forming hydrogels in situ can be used in cell therapy for immobilization and targeted delivery of stem cells.
Subject(s)
Cell Culture Techniques/methods , Cell- and Tissue-Based Therapy/methods , Embryonic Stem Cells/cytology , Animals , Blastocyst/cytology , Chitosan/chemistry , Collagen/chemistry , Hydrogels/chemistry , Mice , Mice, Inbred C57BL , Mice, Inbred CBAABSTRACT
Reconstruction of mouse embryos was performed by injection of donor genetic material from differentiated cells of various types (cumulus cells, cardiomyocytes, and epithelial cells) into recipient cells (mature oocytes and zygotes). A medium for microsurgery was selected, which enhanced survival of both embryonic and somatic cells during the reconstructive manipulations. Special preparation of somatic cells to transplantation was carried out, which employed factors synchronizing the cells in a certain phase of the cell cycle in order to enhance their capacity to maintain the development of reconstructed embryos. The processes of nucleus reprogramming in specialized cells under the action of cytoplasmic factors of oocytes and zygotes were examined. During in vitro culturing of reconstructed embryos, the most successful development was observed in embryos implanted with donor material from cumulus cells. Mouse embryos reconstructed with a certain genome and subsequent production and use of stem cells are considered as the model system for developing the basic principles of replacement therapy.
Subject(s)
Cell Culture Techniques , Embryo, Mammalian/cytology , Embryonic Development , Nuclear Transfer Techniques , Stem Cells/physiology , Animals , Embryo, Mammalian/physiology , Embryo, Mammalian/ultrastructure , Mice , Mice, Inbred BALB CABSTRACT
A controlled study of healing time in donor sites covered with Biocol, a new latex-based material, compared with donor sites covered with Jelonet showed a shorter healing time after treatment with Biocol (P < 0.001). No significant difference in cosmetic results 3 months post wounding was observed. No difference in discomfort was observed.