Analysis of the Use of Sample Size and Effect Size Calculations in a Temporomandibular Disorders Randomised Controlled Trial-Short Narrative Review.

BACKGROUND/OBJECTIVES: Temporomandibular disorder (TMD) is the term used to describe a pathology (dysfunction and pain) in the masticatory muscles and temporomandibular joint (TMJ). There is an apparent upward trend in the publication of dental research and a need to continually improve the quality of research. Therefore, this study was conducted to analyse the use of sample size and effect size calculations in a TMD randomised controlled trial. METHODS: The period was restricted to the full 5 years, i.e., papers published in 2019, 2020, 2021, 2022, and 2023. The filter article type-"Randomized Controlled Trial" was used. The studies were graded on a two-level scale: 0-1. In the case of 1, sample size (SS) and effect size (ES) were calculated. RESULTS: In the entire study sample, SS was used in 58% of studies, while ES was used in 15% of studies. CONCLUSIONS: Quality should improve as research increases. One factor that influences quality is the level of statistics. SS and ES calculations provide a basis for understanding the results obtained by the authors. Access to formulas, online calculators and software facilitates these analyses. High-quality trials provide a solid foundation for medical progress, fostering the development of personalized therapies that provide more precise and effective treatment and increase patients' chances of recovery. Improving the quality of TMD research, and medical research in general, helps to increase public confidence in medical advances and raises the standard of patient care.

Pharmacologically Induced Accommodation Palsy and the Bioelectrical Activity of the Muscular System: A Preliminary Investigation.

The aim of this study was to pharmacologically induce accommodative paralysis and evaluate its effects on the bioelectrical activity of the muscular system. The study included two participant groups: those with myopia and those with normal vision (emmetropes). Electromyographic assessments were performed using the Noraxon Ultium DTS 8-K MR 3 myo Muscle Master Edition system. The muscles analyzed in this study were the temporalis, masseter, sternocleidomastoid, trapezius, abdominal muscles, biceps brachii, and the external oblique muscles of the abdomen. It is important to acknowledge that, based on the current findings, it cannot be definitively stated that the observed effects have clinical significance, and additional studies are encouraged.

Surface Electromyography in Dentistry-Past, Present and Future.

Surface electromyography (sEMG) is a technique for measuring and analyzing the electrical signals of muscle activity using electrodes placed on the skin's surface. The aim of this paper was to outline the history of the development and use of surface electromyography in dentistry, to show where research and technical solutions relating to surface electromyography currently lie, and to make recommendations for further research. sEMG is a diagnostic technique that has found significant application in dentistry. The historical section discusses the evolution of sEMG methods and equipment, highlighting how technological advances have influenced the accuracy and applicability of this method in dentistry. The need for standardization of musculoskeletal testing methodology is highlighted and the needed increased technical capabilities of sEMG equipment and the ability to specify parameters (e.g., sampling rates, bandwidth). A higher sampling rate (the recommended may be 2000 Hz or higher in masticatory muscles) allows more accurate recording of changes in the signal, which is essential for accurate analysis of muscle function. Bandwidth is one of the key parameters in sEMG research. Bandwidth determines the range of frequencies effectively recorded by the sEMG system (the recommended frequency limits are usually between 20 Hz and 500 Hz in masticatory muscles). In addition, the increased technical capabilities of sEMG equipment and the ability to specify electromyographic parameters demonstrate the need for a detailed description of selected parameters in the methodological section. This is necessary to maintain the reproducibility of sEMG testing. More high-quality clinical trials are needed in the future.

Neck pain, disability and mobile phone usage among physiotherapy students - a cross-sectional study.

INTRODUCTION AND OBJECTIVE: Modern mobile devices have become tools used for educational, research, business or recreational purposes. Incorrect position during excessive use of a smartphone can lead to biomechanical changes, the most visible of which is the position of the head in protraction, characterized by the protrusion of the head and neck forward in relation to the shoulder girdle and trunk. The aim of the study was to analyze the relationship between disability, neck pain (NP), use of phones before bedtime, and hours of using smartphones. MATERIAL AND METHODS: The study involved 146 physiotherapy students aged 18-26. Students were asked to complete pain surveys (VAS pain scale), and Neck Disability Index (NDI). Participants were also asked if their pain lasted longer than 3 months, how long they used their smartphone during the day, and whether they used it before bedtime. RESULTS: Statistically significant differences were found between groups with and without neck pain regarding NDI score (p<0.001). Participants who suffered from neck pain longer than 3 months had greater NDI scores (p=0.03), greater intensity of symptoms (p=0.04), greater problems with reading (p<0.01) and driving (p=0.04) than participants who experienced pain for less than 3 months. Using phones before bedtime was related to problems with focusing (p<0.01). There were statistically significant correlations between the time of phone use and disability in terms of reading (p=0.04), focusing (p<0.001), work (p<0.001) and sleeping (p=0.02). CONCLUSIONS: Dysfunctions associated with pain in the cervical section may affect the learning abilities of students and thus the acquisition of professional competencies. Not using a smartphone before bedtime is recommended, as it causes poorer concentration. The longer the time spent using the phone, the more significant the disability.

Myopia & painful muscle form of temporomandibular disorders: connections between vision, masticatory and cervical muscles activity and sensitivity and sleep quality.

The main aim of this study is to evaluate the effects of painful muscle form of temporomandibular disorders and myopia on the connections between the visual organ, the bioelectrical activity and sensitivity of the masticatory and cervical muscles, and sleep quality. Subjects were divided into 4 groups (Myopia & TMDs, Myopia (Without TMDs), Emmetropic & TMDs and Emmetropic (Without TMDs)). The study was conducted in the following order of assessment: examination for temporomandibular disorders, assessment of the muscle activity by electromyograph, pressure pain thresholds examination, ophthalmic examination and completion of the Pittsburgh Sleep Quality Index. It was observed that the Myopia & TMDs group had higher muscle tenderness, higher resting and lower functional muscle bioelectrical activity. The visual organ is clinical related to the masticatory and cervical muscles. TMDs and myopia alter masticatory and cervical muscle activity. The thickness of the choroid in people with myopia is related to muscle tenderness. TMDs and myopia impair sleep quality. It is recommended to determine the number of people with refractive error and its magnitude in the sEMG study in order to be able to replicate the research methodology.

Association between pain intensity, neck disability index, and working conditions among women employed in horticulture.

INTRODUCTION AND OBJECTIVE: One of the most frequent musculoskeletal disorders is neck pain (NP). NP can be associated with occupational activities and is more common among females than males. Horticulture is a branch of agriculture in which work is intensively manual, and characterized by repetitive tasks. The aim of the cross-sectional study was to analyze the association between pain intensity, neck disability index (NDI), and working conditions in terms of selected factors related to work in horticulture. MATERIAL AND METHODS: 44 women employed in horticulture met eligibility criteria (experienced necked pain). Five factors related to working conditions were investigated: work experience, upper extremity position, head position, prophylaxis, and stress frequency. NDI and visual analog scale (VAS) were used to investigate pain intensity and disability. RESULTS: It was found that the position of the upper limb at work and the frequency of stress were significantly associated with the VAS score (p=0.046 and p=0.02, respectively). With regard to NDI total score, a statistically significant association was found between work experience and stress frequency (p=0.02 and p=0.01, respectively). Analysis of the relationship between VAS and NDI total score showed a statistically significant weak positive correlation (R=0.39; p=0.01). CONCLUSIONS: NP and NDI are related to the activities that women working in horticulture have to perform. Stress seems to be an important factor in cervical problems among female workers leading to an increase in NP and disability.

Study protocol: Accommodative effects on the musculoskeletal system.

Accommodation is a phenomenon of the eyeball, which consists of adapting to view objects at different distances. The main aim of this study is to evaluate the effect of accommodations on the musculoskeletal system in myopic and emmetropic subjects. To date, the effect of accommodative paralysis on the musculoskeletal system has not been studied. The research hypothesis based on the current research reports is that accommodation affects the musculoskeletal system in myopic and emmetropic subjects. A smaller aim of the study is to evaluate the effect of unilateral accommodative palsy on the musculoskeletal system in myopic and emmetropic subjects. According to the authors of the previous reports, unilateral accommodative palsy will affect increased musculoskeletal asymmetry in myopic and emmetropic subjects. The surface electromyography (sEMG) of the musculoskeletal system will be performed by using the Noraxon Ultium DTS 8-K MR 3 myo Muscle Master Edition. Cycloftyal (10 mg/ml, eye drops, solution) will be used to paralyze accommodation. After the paralysis of unilateral accommodation, the test will be repeated. Both-sided accommodation will be paralyzed next, and the test will be repeated. The study may provide insight into the effects of accommodation on the musculoskeletal system, and it may also help in understanding the connections between these systems.

Correlation between refractive error, muscle thickness, and bioelectrical activity of selected masticatory muscles.

OBJECTIVE: The aim of this study was to analyze the correlation between the refractive error, muscle thickness, and the bioelectrical activity of selected masticatory and neck muscles in subjects with myopia. METHODS: For bioelectrical activity analysis within the masticatory muscles, an 8-channel BioEMG III electromyograph was used. An M-Turbo ultrasound machine was used to analyze the masticatory and neck muscle thickness. RESULTS: Statistical analysis showed significant positive correlations between masseter muscle thickness at rest on the right side. Statistical analysis showed negative correlations between the digastric muscle and Activity Index within masticatory muscles at rest on the left side when the eyes were closed. CONCLUSIONS: As refractive error increases in myopic subjects, the resting tension on the temporal muscles increases, an increase in masseter muscle thickness is noted, and a decrease in bioelectrical activity on the digastric muscle at rest.

The Influence of Visual Input on Electromyographic Activity and Patterns of Masticatory and Cervical Spine Muscles in Emmetropic Caucasian Subjects by Gender.

(1) Background: The objective of the research was to analyze the change of visual input on electromyographic activity and patterns of masticatory and cervical spine muscles in emmetropic Caucasian subjects by gender. Supposedly, visual input should not influence activity and electromyographic patterns of masticatory and cervical spine muscles in emmetropic Caucasian subjects by gender. (2) Methods: After applying the inclusion criteria, 50 emmetropic Caucasian subjects were included in the study. Four muscle pairs were analyzed: the temporalis muscle (TA), the masseter muscle (MM), the digastric muscle (DA), and the sternocleidomastoid muscle (SCM), during resting and functional activity. (3) Results: It was observed that there were no significant statistical differences in activity and bioelectrical patterns between open and closed eyes in women and men, with the exception of clenching on dental cotton rollers in DA-left and DA mean between tests in women. The observed statistical results had a small effect size, successively equal to 0.32 and 0.29. (4) Conclusions: Changes in the influence of visual input do not affect electromyographic activity and patterns of masticatory and cervical spine muscles in emmetropic Caucasian women and men.

Exploratory Study on Central Sensitization and Bioelectrical Activity of the Selected Masticatory Muscles in Subjects with Myopia.

BACKGROUND: Myopia is one of the most common eye disorders involving abnormal focusing of light rays. The studies recognize the association between the stomatognathic and visual systems. This compound may have a neurological basis associated with disorders such as central sensitization. The main aim of this study was to evaluate the influence of central sensitization on the bioelectrical activity of selected muscles of the masticatory organ in subjects with myopia. METHODS: Selected masticatory and cervical spine muscles were analyzed using an eight-channel BioEMG III electromyograph. Central sensitization was analyzed using the central sensitization inventory. RESULTS: Statistical analysis revealed significantly higher scores on the central sensitization inventory in subjects with axial myopia compared to subjects without refractive error. Repeated positive correlations were observed in the sternocleidomastoid muscle activity and negative correlations in the digastric muscle activity during open and closed eyes in myopic subjects. CONCLUSIONS: Subjects with myopia have an increased score in the central sensitization inventory. The increase in the central sensitization inventory score is connected with the changes within the electromyographic activity of the masticatory and neck muscles. The effect of central sensitization on masticatory muscle activity in myopic subjects requires further study.

The influence of sedentary behaviour on lumbar-pelvic kinematics during squatting and forward bending among physically active students.

Prolonged sitting may involve several mechanisms that make it a risk factor for low back pain. The aim of this study was to investigate lumbar-pelvic kinematics and multifidus muscle (MF) activity during squatting and forward bending in relation to the sedentary behaviour of physically active students. Sixty-three students were divided into two groups according to the time spent in a sitting position during the day: 'high' (>7 h/day); 'low' (≤7 h/day). Lumbar-pelvic ratios, ranges of motion, angular velocities, and MF flexion-relaxation phenomenon were investigated. Data were obtained using the optical motion analysis system, and surface electromyography. The results indicated that lumbar-pelvic ratios during both tasks and velocity of lumbar spine during squatting were significantly greater in the 'high' than in the 'low' sitting group. Muscle activity showed no differences between groups. Prolonged sitting can be considered a factor that slightly, but statistically significantly influences the lumbar-pelvic kinematics in physically active people.Practitioner summary: Lumbar-pelvic kinematics can be altered by prolonged sitting in physically active students. Lumbar-pelvic ratios during squatting and forward bending and lumbar spine velocity during squatting were significantly greater in the 'high' than in the 'low' sitting group. Sedentary behaviour should be considered during an assessment of movement patterns.Abbreviations: BMI: body mass index; ERR: extension-relaxation ratio; FRP: flexion-relaxation phenomenon; FRR: flexion-relaxation ratio; IPAQ: International Physical Activity Questionnaire; LBP: low back pain; METs: metabolic equivalent of tasks; MF: multifidus muscle; PA: physical activity; ROM: range of motion; sEMG: surface electromyography.

Relationship of lumbar-hip kinematics during trunk flexion and sex, body mass index, and self-reported energy expenditure: a cross-sectional analysis.

PURPOSE: Physical activity (PA) is a well-known, simple and effective preventive and therapeutic intervention for low back pain (LBP). In spite of the growing interest in active lifestyles and its benefits, more needs to be known about the relationship between energy expenditure, body mass and lumbar-pelvic kinematics during the forward bending movement in a group of young asymptomatic people who met PA guidelines. Young people can be identified as a future risk group of civilisation diseases and lumbar-hip kinematics can be considered as a predictor of LBP occurrence. The aim of this study was to identify the association of gender, self-reported energy expenditure, body mass index, and lumbar-hip kinematics in young people. METHODS: Sixty-four students at pre-employment stage participated in the study. They declared moderate-to-high PA and activity-induced energy expenditure (AEE) was self-reported. Kinematic data of the lumbar spine, pelvis and hip were collected during forward bending using a 3D motion capture system. RESULTS: Sex was found to be associated with pelvis (ß = -0.38 p = 0.002) and lumbar mobility (ß = 0.49, p < 0.001) during forward bending and BMI was related only to lumbar mobility (ß = -0.41, p = 0.001). Recreation AEE significantly predicted hip flexion mobility (ß = 0.38, p = 0.002). CONCLUSIONS: This study showed that among a sample of physically active young people, BMI, self-reported AEE and sex can partially predict lumbar-hip kinematics during trunk flexion. Recreational PA can be regarded as improving hip mobility and thus making forward bending more effective and less prone to injury.

The Axial Length of the Eyeball and Bioelectrical Activity of Masticatory and Neck Muscles: A Preliminary Report.

Objective: The present study aimed to evaluate the correlation of eye length and bioelectric activity of temporalis, masseter, digastric, and sternocleidomastoid muscles in women with myopia compared to healthy women. Methods: Based on the exclusion and inclusion criteria, 42 women aged 24 years (±2 years) were eligible for the study. Two equally sized groups with myopic (n = 21) and emmetropic healthy subjects (n = 21) were formed. An electromyographic study of the examined muscles was performed in four conditions: at rest, during maximal voluntary clenching in the intercuspal position, during maximal voluntary clenching on dental cotton rollers, and during maximal mouth opening using BioEMG III (BioResearch Associates, Inc. Milwaukee, WI, USA). The IOL Master 500 (Carl Zeiss Meditec, Jena, Germany) was used to examine the eyeball length. Statistical analysis showed significant positive correlations during mouth opening in both groups with open and closed eyes. Results: A greater number of correlations between the analyzed variables was observed in emmetropic women. In almost all cases, the longer axial eye length was associated with an increase in the bioelectrical activity of the analyzed muscles. Significant correlations were most often observed within the masseter and digastric muscles during the maximum mouth opening and at rest. Conclusion: There is a relationship between the bioelectrical activity of the masticatory muscles and the axial length of the eyeball on the same side.

Effects of visual input on changes in the bioelectrical activity of the cervical and masticatory muscles in myopic subjects.

The study aimed to analyze the changes within the bioelectrical activity of the cervical spine and masticatory muscles during the change of visual stimulus-open and closed eyes test. After applying the inclusion criteria, 50 subjects were included in the study, with visual impairment ranging from -0.5 to -5.75 Diopters. Four muscle pairs were analyzed: the anterior part of the temporalis muscle (TA), the superficial part of the masseter muscle (MM), the anterior belly of the digastric muscle (DA), and the middle part of the sternocleidomastoid muscle (SCM) belly during rest, teeth clenching, teeth clenching on dental cotton rollers, and active mouth opening. Statistical analysis showed a significant decrease in the bioelectrical activity during teeth clenching of all analyzed muscles during the closed eyes test. Significant decreases of electromyographic values were also observed during resting activity within TA muscles, during teeth clenching with dental cotton rollers within SCM and DA muscles, and during active mouth opening within the right masseter. Changing the visual stimulus from open eyes to closed eyes in people with myopia affects the bioelectrical activity of the masticatory and cervical spine muscles.

Does Application of Lymphatic Drainage with Kinesiology Taping Have Any Effect on the Extent of Edema and Range of Motion in Early Postoperative Recovery following Primary Endoprosthetics of the Knee Joint?

BACKGROUND: The surgery of knee replacement due to degenerative changes is the last step of the treatment. After surgery, a major problem in patients is pain, swelling, intraarticular hematoma, and the restriction of the mobility of the joint. The aim of this work was to determine the effect of Kinesio Taping (KT) on reducing edema of the subcutaneous tissue and improving the range of motion in the joint. METHODS: 82 patients were qualified for the study. After surgery, 42 patients received postoperative edema treatment with KT bands, and 40 patients did not receive the treatment. The swelling thickness and range of mobility were measured on the third and eighth days after the operation. RESULTS: A statistical difference between the longitudinal measurements of the KT group and the group without KT application was shown at the level of the fibula head, 25 mm below the fibula neck, and 50 mm below the fibular neck. There were no statistically significant differences in the change in knee angle between the applied and non-applied patients. CONCLUSION: The lymphatic application technique KT influences the absorption of subcutaneous edema after primary knee joint replacement surgery but has no influence on mobility.

Masticatory Muscle Thickness and Activity Correlates to Eyeball Length, Intraocular Pressure, Retinal and Choroidal Thickness in Healthy Women versus Women with Myopia.

This study aims to examine the correlations between masticatory and neck muscle thickness and activity versus eyeball length, retinal thickness, choroidal thickness, and intraocular pressure in healthy women versus women with myopia. The study group consisted of 21 women aged 24 years and a control group of 19 women (mean age 23 years). For bioelectrical activity analysis within the temporalis anterior, the superficial part of the masseter muscle, the middle part of the sternocleidomastoid muscle, and the anterior belly of the digastric muscle, an eight-channel BioEMG III electromyograph were used. An M-Turbo ultrasound machine was used to analyze masticatory and neck muscle thickness. The eyeball length was examined by IOL Master 500; choroidal and retinal thickness by Optovue Angiovue; and intraocular pressure by Tono-Pen XL. Refractive errors are related to differences in muscle thickness and electromyographic activity. Bioelectrical activity within the temporalis anterior seems to be associated with ocular length, retinal thickness, and choroidal thickness in women with myopia.

Assessment of Growth Changes in the Width of Dental Arches Caused by Removable Appliances over a Period of 10 Months in Children with Malocclusion.

(1) Background: A large number of patients of orthodontic clinics are diagnosed with improper jaw relationships. Intraoral scanners have become an important part of orthodontic practice and provide an opportunity to measure the changes in the width of dental arches. The purpose of the study was to evaluate the impact of removable appliances used over a 10-month period on growth changes in children with narrowed jaw dimensions. (2) Methods: Twenty four patients were included in the study (a study group-patients, treated with removable appliances in the upper dental arch for a minimum of 10 months; a control group-patients with no craniofacial abnormalities and who did not require orthodontic treatment). A panoramic radiograph and digital intraoral scan were taken, followed by palatal width measurements in Ortho-CAD before treatment, and after a period of 10 months of treatment with removable appliances. (3) Results: After a period of 10 months of the treatment, the study group had a statistically significantly greater mean change in the anterior width of the upper dental arch than the control group. (4) Conclusions: The use of removable appliances in children with narrowed maxillary transverse dimension contributes to offsetting growth changes in comparison to children with normal occlusion.

The Influence of Visual Input on Electromyographic Patterns of Masticatory and Cervical Spine Muscles in Subjects with Myopia.

This study aimed to analyze the change of visual input on electromyographic patterns of masticatory and cervical spine muscles in subjects with myopia. After applying the inclusion criteria, 50 subjects (18 males and 32 females) with myopia ranging from -0.5 to -5.75 Diopters (D), were included in the study. Four muscle pairs were analyzed: the anterior part of the temporalis muscle (TA), the superficial part of the masseter muscle (MM), the anterior belly of the digastric muscle (DA), and the middle part of the sternocleidomastoid muscle belly (SCM) during resting and functional activity. Statistical analysis showed a significant decrease within functional indices (FCI) for the sternocleidomastoid muscle (FCI SCM R, FCI SCM L, FCI SCM total) during clenching in the intercuspal position with eyes closed compared to eyes open. During maximum mouth opening, a statistically significant increase of functional opening index for the left temporalis muscle (FOI TA L) was observed. Within the activity index (AcI), there was a statistically significant decrease during clenching on dental cotton rollers with eyes closed compared to eyes open.

Influence of regular climbing on depression, generalized anxiety and lower back pain.

INTRODUCTION AND OBJECTIVE: Anxiety and depression disorders are increasingly affecting society. Anxiety disorders are the most prevalent psychiatric disorders, and depressive disorders are increasing in society worldwide. Lower back pain is one of the common health problems experienced by 50-80% of adults at some point in their lives. The aim of the study was to assess the climbing environment in terms of depression, generalized anxiety and pain in the lumbar spine. MATERIAL AND METHODS: 139 people training on climbing walls Lublin in December 2019 were invited to participate in the study. After applying exclusion criteria, 113 people with an average age of 30±8 years, 88 men (average age: 30 years) and 35 women (average age: 30 years) were qualified for the study. They were examined by the author's own questionnaire and the PHQ-9, GAD-7 and ODI questionnaires. Statistical analyses were applied and the significance threshold was set at 0.05. RESULTS: Overall percentage results suggest mild depression at 27%, moderate anxiety at 27%, and mild back pain at 26% in the group of climbers tested. Climbing level and climbing sub-discipline did not affect the average results of PHQ-9, GAD-7 (p>0.05). Back pain was more common in boulder climbers (p<0.05). CONCLUSIONS: The level of sport climbing did not affect the level of depression, generalized anxiety, and back pain. The sport climbing level did not affect the incidence of injuries. The association in climbing clubs is connected with overcoming more difficult climbing routes and a higher level of generalized anxiety. Bouldering was associated with a greater level of pain in the lumbar spine and more injuries.

Alternative Splicing Role in New Therapies of Spinal Muscular Atrophy.

It has been estimated that 80% of the pre-mRNA undergoes alternative splicing, which exponentially increases the flow of biological information in cellular processes and can be an attractive therapeutic target. It is a crucial mechanism to increase genetic diversity. Disturbed alternative splicing is observed in many disorders, including neuromuscular diseases and carcinomas. Spinal Muscular Atrophy (SMA) is an autosomal recessive neurodegenerative disease. Homozygous deletion in 5q13 (the region coding for the motor neuron survival gene (SMN1)) is responsible for 95% of SMA cases. The nearly identical SMN2 gene does not compensate for SMN loss caused by SMN1 gene mutation due to different splicing of exon 7. A pathologically low level of survival motor neuron protein (SMN) causes degeneration of the anterior horn cells in the spinal cord with associated destruction of α-motor cells and manifested by muscle weakness and loss. Understanding the regulation of the SMN2 pre-mRNA splicing process has allowed for innovative treatment and the introduction of new medicines for SMA. After describing the concept of splicing modulation, this review will cover the progress achieved in this field, by highlighting the breakthrough accomplished recently for the treatment of SMA using the mechanism of alternative splicing.