An improved source of spin-polarized electrons based on spin exchange in optically pumped rubidium vapor.

We have improved a polarized electron source in which unpolarized electrons undergo collisions with a mixture of buffer gas molecules and optically spin-polarized Rb atoms. With a nitrogen buffer gas, the source reliably provides spin polarization between 15% and 25% with beam currents >4 µA. Vacuum pump upgrades mitigate problems caused by denatured diffusion pump oil, leading to longer run times. A new differential pumping scheme allows the use of higher buffer gas pressures up to 800 mTorr. With a new optics layout, the Rb polarization is continuously monitored by a probe laser and improved pump laser power provides more constant high polarization. We have implemented an einzel lens to better control the energy of the electrons delivered to the target chamber and to preferentially select electron populations of higher polarization. The source is designed for studies of biologically relevant chiral molecule samples, which can poison photoemission-based GaAs polarized electron sources at very low partial pressures. It operates adjacent to a target chamber that rises to pressures as high as 10-4 Torr and has been implemented in a first experiment with chiral cysteine targets.

A simple, isotropic depolarized light source.

Unpolarized light can be an important tool in optical experiments. Producing it, however, can prove to be a challenge. Natural sources of light that are commonly thought of as unpolarized are, in fact, either weakly polarized or not practical sources of light in a laboratory setting. Standard, commercially available light depolarizers produce unpolarized light only after the polarization state of the light across the diameter of the output beam has been averaged. Locally, such beams are highly polarized. In this work, we report a simple, low cost light depolarizer capable of producing light with a total polarization of less than 1% for a 15-mm diameter output beam. Based upon diffuse scattering, the light transmitted through the depolarizer discussed here produces only small polarizations locally, with the total polarization for a 1.25-mm diameter area being <6%. The effects of the depolarizer on the transmitted beam's intensity are also reported.

A search for chiral asymmetry in secondary electron emission from cysteine induced by longitudinally polarized electrons.

We performed experiments searching for chirality-dependent secondary electron emission for a 141 eV longitudinally spin-polarized electron beam incident on a thick solid cysteine target. We determined the secondary electron yield by measuring the positive current produced when the cysteine target was negatively biased. No spin-dependent effects to a level of 10-3 were found for the secondary electron emission yield.

Non-Poissonian Ultrashort Nanoscale Electron Pulses.

The statistical character of electron beams used in current technologies, as described by a stream of particles, is random in nature. Using coincidence measurements of femtosecond pulsed electron pairs, we report the observation of sub-Poissonian electron statistics that are nonrandom due to two-electron Coulomb interactions, and that exhibit an antibunching signal of 1 part in 4. This advancement is a fundamental step toward observing a strongly quantum degenerate electron beam needed for many applications, and in particular electron correlation spectroscopy.

Helmholtz spacing of thin rectangular magnetic field coils.

In this Note, we discuss the Helmholtz spacing for a pair of thin rectangular coils of arbitrary aspect ratio and consider how best to use such coils to compensate for Earth's magnetic field along the coils' Cartesian symmetry axes. Such coils are frequently used in conjunction with charged-particle beam machines. The Helmholtz spacing varies non-monotonically between that for square coils and that for four optimally spaced infinite wires. We consider other coil spacings that extend the length over which the field varies by less than some tolerance along the Cartesian symmetry axes. The calculations also provide a convenient means to evaluate when the length of the coils is sufficiently long to be considered infinite at the center point within a fixed tolerance.

Demonstration of vacuum strain effects on a light-collection lens used in optical polarimetry.

The precision by which an electron spin polarization measurement can be made using a noble-gas polarimeter depends directly on the accuracy of a light-polarization measurement. Since the electron-noble gas collisions occur in a vacuum chamber and the optical polarimeter is generally outside the chamber, this work examines the effect the vacuum window has on the perceived optical polarization. A model light source, lens system, and optical polarimeter are used that approximate the situation found in a typical atomic physics experiment. It was demonstrated that a pressure difference of 1 atm on a lens will alter the perceived polarization by as much as 0.05% with typical borosilicate (BK) lenses. This effect was demonstrated to scale with the thickness of the lens used and changes signs when the direction of the stress is reversed.

Alignment of the (3d

We measure the integrated Stokes parameters of light from Zn (4s4p)4^{3}P_{0,1}-(4s5s)5^{3}S_{1} transitions excited by a transversely polarized electron impact at energies between 7.0 and 8.5 eV. Our results for the electron-polarization-normalized linear polarization Stokes parameter P_{2}, between incident electron energies 7.0 and 7.4 eV, are consistent with zero, as required by basic angular-momentum coupling considerations and by recent theoretical calculations. They are in qualitative disagreement with previous experimental results for the P_{2} parameter.

A cylindrically symmetric "micro-Mott" electron polarimeter.

A small, novel, cylindrically symmetric Mott electron polarimeter is described. The effective Sherman function, Seff, or analyzing power, for 20 kV Au target bias with a 1.3 keV energy loss window is 0.16 ± 0.01, where uncertainty in the measurement is due primarily to uncertainty in the incident electron polarization. For an energy loss window of 0.5 keV, Seff reaches its maximum value of 0.24 ± 0.02. The device's maximum efficiency, I/Io, defined as the detected count rate divided by the incident particle rate, is 3.7 ± 0.2 × 10(-4) at 20 keV. The figure-of-merit of the device, η, is defined as Seff (2)IIo and equals 9.0 ± 1.6 × 10(-6). Potential sources of false asymmetries due to detector electronic asymmetry and beam misalignment have been investigated. The new polarimeter's performance is compared to published results for similar compact retarding-field Mott polarimeters, and it is concluded that this device has a relatively large Seff and low efficiency. SIMION(®) electron trajectory simulations and Sherman function calculations are presented to explain the differences in performance between this device and previous designs. This design has an Seff that is insensitive to spatial beam fluctuations and, for an energy loss window >0.5 keV, negligible background due to spurious ion and X-ray production at the target.

Anomalously Large Chiral Sensitivity in the Dissociative Electron Attachment of 10-Iodocamphor.

We have studied dissociative electron attachment (DEA) between low energy (≤0.6 eV) longitudinally polarized electrons and gas-phase chiral targets of 3-bromocamphor (C_{10}H_{15}BrO), 3-iodocamphor (C_{10}H_{15}IO), and 10-iodocamphor. The DEA rate depends on the sign of the incident electron helicity for a given target handedness, and it varies with both the atomic number (Z) and location of the heaviest atom in the molecule. While simple dynamic mechanisms can account for the asymmetry dependence on Z, they fail to explain the large asymmetry variation with the heavy atom location.

New technique for the reduction of helicity-correlated instrumental asymmetries in photoemitted beams of spin-polarized electrons.

We present a new optical system that significantly reduces helicity-dependent instrumental intensity asymmetries. It is an extension of a previous scheme [Appl. Opt.47, 2465 (2008)], where one laser beam is split using a polarizing beam splitter into two with orthogonal linear polarizations. The beams are sent through a chopper, allowing only one to pass at a time. The two temporally separated beams are then spatially recombined using a second beam splitter. A liquid crystal retarder preceding the first beam splitter controls the relative intensity of the two oppositely polarized beams, allowing reduction of instrumental asymmetries. This system has been modified to include a spatial filter and a Pockels cell placed after the second beam splitter to act as a second active polarization element. Using this method, we can control instrumental asymmetries to ∼5×10(-7) in 1 h of data taking, which is comparable to the precision achieved in "second-generation" high energy electron-nuclear scattering parity violation experiments.

Chirally sensitive electron-induced molecular breakup and the Vester-Ulbricht hypothesis.

We have studied dissociative electron attachment in sub-eV collisions between longitudinally polarized electrons and chiral bromocamphor molecules. For a given target enantiomer, the dissociative Br anion production depends on the helicity of the incident electrons, with an asymmetry that depends on the electron energy and is of order 3×10^{-4}. The existence of chiral sensitivity in a well-defined molecular breakup reaction demonstrates the viability of the Vester-Ulbrict hypothesis, namely, that the longitudinal polarization of cosmic beta radiation was responsible for the origins of biological homochirality.

Compact inline optical electron polarimeter.

A compact optical electron polarimeter using a helium target is described. It offers a maximum fluorescence detection efficiency of ~20 Hz/nA, which is an order of magnitude higher than that of earlier designs. With an argon target, this device is expected to have a polarimetric figure-of-merit of 270 Hz/nA. By relying on a magnetic field to guide a longitudinally spin-polarized electron beam, the present instrument employs fewer electrodes. It also uses a commercially available integrated photon counting module. These features allow it to occupy a smaller volume and make it easier to operate.

Threshold alignment reversal and circularly polarized fluorescence in rotationally resolved H2.

Using H2 and D2 targets, we have measured the polarization of Fulcher-band fluorescence resulting from spin-polarized electron-impact excitation of vibrationally and rotationally resolved d 3Π(u) → a 3Σ(g)+ transitions for incident electron energies from 14.3 to 28.5 eV. Near threshold, the linear polarization P1 descends from positive values through zero to negative values, indicating a dynamic production of M(N)=0 states. The circular polarization P3 is measured to be nonzero, indicating the orientation of rotationally resolved molecular states. For Q-branch transitions, P3 is consistent with theory based on Hund's case (b) coupling. The R-branch P3 values do not agree with theory equally well, indicating the effect of Σ-symmetry perturbation of the parent d 3Π(u)+ state.

Active feedback scheme for minimization of helicity-dependent instrumental asymmetries.

A method for the active feedback reduction of optical instrumental intensity asymmetries is presented. It is based on the fast chopping of two spatially separated beams of light with orthogonal linear polarizations that are recombined and passed through a quarter-wave plate to yield a single beam with rapidly flipping helicity. Active electro-optic feedback has been successfully employed to maintain this asymmetry below 10(-5).

Assignments of normally unoccupied orbitals to the temporary negative ion states of several lanthanide NMR shift reagents and comments on resonance involvement in electron circular dichroism.

We have measured the total electron scattering cross sections of several NMR shift reagent molecules X(hfc)3, where X = Yb, Er, Eu and Pr, by means of electron transmission spectroscopy (ETS) to determine their vertical attachment energies. A strong low-energy resonance (<1 eV) is observed in all of the compounds except for Yb(hfc)3. We explain this anomaly in terms of the ground-state electron configuration of each molecule. Also, with the aid of restricted open-shell Hartree-Fock (ROHF) calculations on analogous molecules with truncated fluorocarbon chains, we have assigned specific normally unoccupied orbitals to the resonances observed in ETS. To our knowledge, these molecules are the largest for which this procedure has been successfully completed. Nolting et al. (J. Phys. B 1997, 30, 5491) have demonstrated that the above NMR shift reagents exhibit electron circular dichroism (ECD) between 1 and 10 eV. Using our new total cross section data, we comment on the possibility of resonance involvement in the generation of ECD.

Detection of U.S., Lelystad, and European-like porcine reproductive and respiratory syndrome viruses and relative quantitation in boar semen and serum samples by real-time PCR.

Transmission of porcine reproductive and respiratory syndrome virus (PRRSV) via boar semen has been documented. Since semen is widely disseminated for artificial insemination and the virus can cause significant health and economic consequences, it is essential to have well-validated, rapid diagnostic techniques to detect and quantitate the virus for diagnostic and research purposes. Previously, boar semen was tested by a nested PCR (nPCR) assay which was compared to the "gold standard" swine bioassay. A correlation of 94% was observed, indicating that, most of the time, PCR detected infectious virus. Subsequently, a real-time PCR targeting the 3' untranslated region of the PRRSV genome was compared with nPCR by testing 413 serum and semen samples from PRRSV-inoculated and control boars. There was 95% agreement between the results of the two tests, with the majority of samples with discordant results containing virus at the lower range of detection by the assays. The virus in all samples was quantitated by using a standard curve obtained by serial dilution of an in vitro transcript. By using the in vitro transcript, the lower limit of sensitivity was observed to be approximately 33 copies/ml. Reactivity with a panel of more than 100 PRRSV isolates from various geographical regions in the United States was also documented. No reactivity with nine nonrelated swine viruses was noted. A real-time PCR was also developed for the detection of the European Lelystad virus and the European-like PRRSV now found in the United States. In six of six PRRSV-inoculated boars, peak levels of viremia occurred at 5 days postinoculation (DPI) and were most consistently detectable throughout 22 DPI. In five of six boars, PRRSV was shed in semen for 0 to 2 days during the first 10 DPI; however, one of six boars shed the virus in semen through 32 DPI. Therefore, in general, the concentration and duration of PRRSV shedding in semen did not correlate with the quantity or duration of virus in serum. These differences warrant further studies into the factors that prevent viral replication in the reproductive tract.

Spin-exchange-induced circularly polarized molecular fluorescence.

We have measured the circular polarization of light emitted from both atomic H and molecular H2 after bombarding H2 with longitudinally polarized electrons. For both atomic and molecular fluorescence near threshold we observe a circular polarization as great as 10% of the electron polarization. This represents the first direct observation of spin transfer in electron-molecule collisions.

Effect of relativistic many-electron interactions on photoelectron partial wave probabilities.

We obtain relative cross sections for the production of photoelectrons with specific angular momentum quantum numbers. These cross sections are obtained from the polarization analysis of the visible fluorescence of ions produced when circularly polarized vacuum ultraviolet radiation photoionizes ground state Ar. The ratio of cross sections for the production of photoelectrons with the same orbital angular momentum but different total angular momenta shows strong deviations from the statistical ratio, demonstrating the importance of relativistic interactions in many-electron photoionization dynamics.

Induction of CD4(+) T cell-dependent CD8(+) type 1 responses in humans by a malaria DNA vaccine.

We assessed immunogenicity of a malaria DNA vaccine administered by needle i.m. or needleless jet injection [i.m. or i.m./intradermally (i.d.)] in 14 volunteers. Antigen-specific IFN-gamma responses were detected by enzyme-linked immunospot (ELISPOT) assays in all subjects to multiple 9- to 23-aa peptides containing class I and/or class II restricted epitopes, and were dependent on both CD8(+) and CD4(+) T cells. Overall, frequency of response was significantly greater after i.m. jet injection. CD8(+)-dependent cytotoxic T lymphocytes (CTL) were detected in 8/14 volunteers. Demonstration in humans of elicitation of the class I restricted IFN-gamma responses we believe necessary for protection against the liver stage of malaria parasites brings us closer to an effective malaria vaccine.