Estimation Based on Emission Wavelength of Dabigatran Etexilate Mesylate in Bulk and Capsule Dosage Form.

A simple, rapid, specific and highly sensitive spectrofluorimetric method has been developed for the quantification of dabigatran etexilate mesylate in bulk and capsule dosage form. A linear relationship was found between fluorescence intensity and concentration in the range of 0.01-1.0 µg/ml in dimethyl sulphoxide as solvent at an emission wavelength of 391 nm after excitation at 334 nm, with a good correlation coefficient (0.989). The detection and quantification limits were found to be 0.005 and 0.015 µg/ml, respectively. The proposed method was applied for dabigatran etexilate mesylate capsules, results reveal with percentage recovery of 102% and percentage relative standard deviation values were found to be less than 2 for accuracy and precision studies. The proposed method was validated for linearity, range, accuracy, precision, limit of detection and quantification according to International Conference on Harmonization guidelines. Statistical analysis of the results revealed high accuracy and good precision. The suggested procedures could be used for the determination of dabigatran etexilate mesylate in bulk and capsule dosage form in quality control laboratories of industries as well as in academic institutions.

Seed-specific increased expression of 2S albumin promoter of sesame qualifies it as a useful genetic tool for fatty acid metabolic engineering and related transgenic intervention in sesame and other oil seed crops.

The sesame 2S albumin (2Salb) promoter was evaluated for its capacity to express the reporter gusA gene encoding ß-glucuronidase in transgenic tobacco seeds relative to the soybean fad3C gene promoter element. Results revealed increased expression of gusA gene in tobacco seed tissue when driven by sesame 2S albumin promoter. Prediction based deletion analysis of both the promoter elements confirmed the necessary cis-acting regulatory elements as well as the minimal promoter element for optimal expression in each case. The results also revealed that cis-regulatory elements might have been responsible for high level expression as well as spatio-temporal regulation of the sesame 2S albumin promoter. Transgenic over-expression of a fatty acid desaturase (fad3C) gene of soybean driven by 2S albumin promoter resulted in seed-specific enhanced level of α-linolenic acid in sesame. The present study, for the first time helped to identify that the sesame 2S albumin promoter is a promising endogenous genetic element in genetic engineering approaches requiring spatio-temporal regulation of gene(s) of interest in sesame and can also be useful as a heterologous genetic element in other important oil seed crop plants in general for which seed oil is the harvested product. The study also established the feasibility of fatty acid metabolic engineering strategy undertaken to improve quality of edible seed oil in sesame using the 2S albumin promoter as regulatory element.

Comparative study of Copper IUDs.

PIP: A comparative study of 4 copper IUDs, Cu T 200, Cu T 380, and Cu Y, was undertaken in the Peripheral Contraceptive Testing Unit of Indian Council of Medical Research at Madurai. 250 women wearing Cu T 200, 227 wearing Cu T 220, 281 Cu T 380 A and ll9 Cu Y were followed up at 1, 3, 6, 9, 12, and 18 months. Continuation rates were lower with Cu Y and Cu T 380. Expulsion and termination rates due to expulsion were higher with Cu T 200 and Cu T 380 A. Menstrual disorders were highest with Cu T 380A. There were 2 pregnancies with Cu T 200, none in the other groups. Expulsion rates were the lowest for Cu Y, Cu T 220 second lowest. Menstrual disorders occurred least among Cu T 200 users. Minor side effects, abdominal pain, backache, leucorrhea, did not vary with device used. The removal rate for menstrual side effects was highest for Cu T 380 A, 3x that of Cu T 200. Cu T 220 and Cu T 200 had similar removal rates. The Cu T 200 retains its contraceptive efficacy up to a period of 4 years.^ieng