In vitro effect of insulin on rats erythrocytes rheological behaviour.

Blood viscosity is an important cardiovascular risk factor that might be related to diabetes complications. Hyperinsulinemia has been suggested as "the most important candidate" to characterise diabetes as a risk factor for cardiovascular disease. There is no evidence of the beneficial effect of insulin on type 2 diabetes erythrocytes in patients without cardiovascular disease, whereas the opposite is observed in those with cardiovascular disease. In the present study we analysed the in vitro effect of different doses of insulin on red blood cell rheological aspects in an obesity model. Previous studies carried out in beta strain rats had shown that this strain possess insulin blood levels higher than the ones observed in alpha strain (eumetabolic), as well as blood hyperviscosity and erythrocyte deformability decrease. Our results points out that in vitro insulin produced an increase in erythrocyte aggregability, although it did not modified either their osmotic fragility or erythrocyte deformability estimated by viscometry, even against decreased viscosity of treated erythrocytes submitted to increased shear rate.

Oleoyl-estrone metabolic effects in relation with caloric restriction in inbred Beta rats with spontaneous obesity and type 2 diabetes.

Spontaneously hypertriacylglycerolemic obese and diabetic inbred IIM Beta rats were treated with oleoylestrone for 10 days. Pair-feeding was performed to determine some oleoyl-estrone effects dependent on the caloric restriction it promotes. Twenty-five 200 day-old Beta males receiving a daily gavage of 0.2 ml sunflower oil were divided into the following groups: 1) daily dose of 10 nmol/g oleoyl-estrone; 2) pair-fed; 3) control. The variables measured were: whole body protein, water and lipid; retroperitoneal and epididymal fat depot weights; plasma urea, glucose, insulin, triacylglycerols and cholesterol. Biomass and food intake were assessed daily. Oleoyl-estrone and pair-fed groups expressed similar variations in body composition and significant body weight losses due to reduction in food intake. Oleoyl-estrone and pair-fed treatments significantly reduced retroperitoneal fat depot weights, but not epididymal ones. In oleoyl-estrone and pair-fed groups hyperglycemia decreased and insulinemia lowered significantly. Plasma normal total cholesterolemia and hypertriacylglycerolemia values typical of Beta rats decreased strongly compared to controls, though attaining significantly different values between oleoyl-estrone and pair-fed groups. Plasma total cholesterol appeared as more sensitive to caloric restriction than triacylglycerols through a specific oleoyl-estrone-mediated effect.

Oleoyl-estrone metabolic effects in relation with caloric restriction in inbred Beta rats with spontaneous obesity and type 2 diabetes.

Spontaneously hypertriacylglycerolemic obese and diabetic inbred IIM Beta rats were treated with oleoylestrone for 10 days. Pair-feeding was performed to determine some oleoyl-estrone effects dependent on the caloric restriction it promotes. Twenty-five 200 day-old Beta males receiving a daily gavage of 0.2 ml sunflower oil were divided into the following groups: 1) daily dose of 10 nmol/g oleoyl-estrone; 2) pair-fed; 3) control. The variables measured were: whole body protein, water and lipid; retroperitoneal and epididymal fat depot weights; plasma urea, glucose, insulin, triacylglycerols and cholesterol. Biomass and food intake were assessed daily. Oleoyl-estrone and pair-fed groups expressed similar variations in body composition and significant body weight losses due to reduction in food intake. Oleoyl-estrone and pair-fed treatments significantly reduced retroperitoneal fat depot weights, but not epididymal ones. In oleoyl-estrone and pair-fed groups hyperglycemia decreased and insulinemia lowered significantly. Plasma normal total cholesterolemia and hypertriacylglycerolemia values typical of Beta rats decreased strongly compared to controls, though attaining significantly different values between oleoyl-estrone and pair-fed groups. Plasma total cholesterol appeared as more sensitive to caloric restriction than triacylglycerols through a specific oleoyl-estrone-mediated effect.

Oleoyl-estrone metabolic effects in relation with caloric restriction in inbred beta rats with spontaneous obesity and type 2 diabetes

Rats endocriadas de la línea IIMBeta con obesidad, hipertriacilglicerolemia y diabetes espontáneas fueron tratadas con oleoil-estrona durante 10 días. Un grupo con restricción alimentaria fue incluido en el estudio a fin de aislar algunos efectos de la oleoil-estrona dependientes de la restricción calórica que ésta promueve. Veinticinco ratas Beta macho de 200 dias de edad a los que se suministró 0.2 ml de aceite de girasol por día se dividieron en los seguientes grupos: (1) dosis diaria de 10 nmol/g de oleoil-estrona ; (2) restringido; (3) control. Las variables medidas fueron: proteínas corporales totales, agua y lípidios; pesos de los panículos adiposos retroperitoneal y epididimario; urea, glucosa, insulina, colesterol y triacilgliceroles plasmáticos. Los valores de biomasa y de ingesta de alimentos se registraron diariamente. Los grupos tratados con oleoil-estrona y restringido mostraron variaciones similares en composicíon corporal y disminuciones significativas en peso corporal debidas a reducciones en la ingesta de alimentos. Los tratamientos con oleoil-estrona y restringido disminuyeron significamente los pesos de los panículos adiposos retroperitoneales, pero no de los epididimarios. En los grupos tratados con oleoil-estrona y restringido la hiperglicemia disminuyó y la insulinemia lo hizo en forma significativa. Los valores de colesterol total plasmático normal y la hipertriacilglicerolemia característicos de las ratas Beta disminuyeron fuertemente comparados con los controles, aunque alcanzaron valores significa-tivamente diferentes entre los grupos tratados con oleoil-estrona y restringido. El colesterol total plasmático aparece como más sensible a la restricción calórica que los traicilgliceroles a través de un efecto específico mediado por la oleoil-estrona.

Oleoyl-estrone metabolic effects in relation with caloric restriction in inbred beta rats with spontaneous obesity and type 2 diabetes

Rats endocriadas de la línea IIMBeta con obesidad, hipertriacilglicerolemia y diabetes espontáneas fueron tratadas con oleoil-estrona durante 10 días. Un grupo con restricción alimentaria fue incluido en el estudio a fin de aislar algunos efectos de la oleoil-estrona dependientes de la restricción calórica que ésta promueve. Veinticinco ratas Beta macho de 200 dias de edad a los que se suministró 0.2 ml de aceite de girasol por día se dividieron en los seguientes grupos: (1) dosis diaria de 10 nmol/g de oleoil-estrona ; (2) restringido; (3) control. Las variables medidas fueron: proteínas corporales totales, agua y lípidios; pesos de los panículos adiposos retroperitoneal y epididimario; urea, glucosa, insulina, colesterol y triacilgliceroles plasmáticos. Los valores de biomasa y de ingesta de alimentos se registraron diariamente. Los grupos tratados con oleoil-estrona y restringido mostraron variaciones similares en composicíon corporal y disminuciones significativas en peso corporal debidas a reducciones en la ingesta de alimentos. Los tratamientos con oleoil-estrona y restringido disminuyeron significamente los pesos de los panículos adiposos retroperitoneales, pero no de los epididimarios. En los grupos tratados con oleoil-estrona y restringido la hiperglicemia disminuyó y la insulinemia lo hizo en forma significativa. Los valores de colesterol total plasmático normal y la hipertriacilglicerolemia característicos de las ratas Beta disminuyeron fuertemente comparados con los controles, aunque alcanzaron valores significa-tivamente diferentes entre los grupos tratados con oleoil-estrona y restringido. El colesterol total plasmático aparece como más sensible a la restricción calórica que los traicilgliceroles a través de un efecto específico mediado por la oleoil-estrona. (AU)