ABSTRACT
In this paper, we present a review of current literature on the application of intraoperative fluorescence diagnosis and fluorescence spectroscopy using 5-aminolevulinic acid in surgery for various types of brain tumors, both alone and in combination with other neuroimaging methods. Authors' extensive experience with these methods allowed them to develop a set of clinical guidelines for the use of intraoperative fluorescence diagnosis and fluorescence spectroscopy in surgery of brain tumors.
Subject(s)
Aminolevulinic Acid , Brain Neoplasms/pathology , Glioma/pathology , Monitoring, Intraoperative/methods , Neuroimaging/methods , Neurosurgical Procedures , Photosensitizing Agents , Brain Neoplasms/surgery , Glioma/surgery , Humans , Microscopy, Fluorescence , Practice Guidelines as Topic , Spectrometry, FluorescenceABSTRACT
The visual evaluation of the degree of GBM resection based upon postoperative enhanced MRI is still difficult. It is explained by intricate complex of tumor residual fragments, blood cells, hemostatic tissues and perifocal edema that located in the postoperative area. We introduce the new software-based method for postoperative MRI data's objective estimation. Five independent specialists had examined 16 patients' MRI data (including 12 GBM and 4 patients with noninfiltrative intracerebral tumors) and tested the method's specificity and sensitivity against the enhanced residual tumor (ERT). Our software determines the 100% sensitivity and specificity against hemostatic agent Surgicel, the high recurrence rate of results while estimating the volume of ERT (0,14 ± 0,02 cm³) and low considerable time (5,21 ± 0,14 min). The software was can be used both daily practice and research of malignant glioma management.
Subject(s)
Brain Neoplasms/surgery , Glioblastoma/surgery , Software , Surgery, Computer-Assisted/methods , Algorithms , Case-Control Studies , Humans , Magnetic Resonance Imaging , Sensitivity and SpecificityABSTRACT
An adaption of an RNA/RNA duplex, base pair-mismatch assay is capable of detecting rifampin resistance in Mycobacterium tuberculosis. The specificity and sensitivity of the mismatch assay in detecting rifampin resistance were 100% and 96%, respectively, when tested against 46 rifampin-resistant and rifampin-susceptible strains of M. tuberculosis. By use of a range of mycobacterial and nonmycobacterial prokaryote pathogens, the mismatch assay was shown to be specific for M. tuberculosis and Mycobacterium bovis. The assay is cost-effective compared with DNA sequencing and other molecular methods and is simple to perform and interpret. Furthermore, the assay can return a result within 24 h after receipt of an isolated organism and potentially can be used directly with smear-positive specimens.
