ABSTRACT
Four healthy, male, adult African lions (Panthera leo) were presented for vasectomy, which was performed for management purposes. After immobilisation with medetomidine and tiletamine/zolazepam the lions were intubated and anaesthesia was maintained with isoflurane. In each animal, the ductus deferens was located bilaterally, dissected and transected. Following ligation, a technique commonly used in human medicine called fascial interposition, was used to decrease the chances of recanalisation. Using this technique, the prostatic end of the ductus was fixated outside the tunica vaginalis, while the testicular end remained within the tunic. Histopathology was performed in all cases to confirm the presence of the ductus deferens tissue. During the follow up, twelve months later, no complications were noticed by the owner and no new litters have been born since.
