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1.
Oncogene ; 30(10): 1147-58, 2011 Mar 10.
Article in English | MEDLINE | ID: mdl-21151176

ABSTRACT

In contrast to other cytotoxic agents including anthracyclins and oxaliplatin (OXP), cisplatin (CDDP) fails to induce immunogenic tumor cell death that would allow to stimulate an anticancer immune response and hence to amplify its therapeutic efficacy. This failure to induce immunogenic cell death can be attributed to CDDP's incapacity to elicit the translocation of calreticulin (CRT) from the lumen of the endoplasmic reticulum (ER) to the cell surface. Here, we show that, in contrast to OXP, CDDP is unable to activate the protein kinase-like ER kinase (PERK)-dependent phosphorylation of the eukaryotic translation initiation factor 2α (eIF2α). Accordingly, CDDP also failed to stimulate the formation of stress granules and macroautophagy, two processes that only occur after eIF2α phosphorylation. Using a screening method that monitors the voyage of CRT from the ER lumen to the cell surface, we identified thapsigargin (THAPS), an inhibitor of the sarco/ER Ca(2+)-ATPase as a molecule that on its own does not stimulate CRT exposure, yet endows CDDP with the capacity to do so. The combination of ER stress inducers (such as THAPS or tunicamycin) and CDDP effectively induced the translocation of CRT to the plasma membrane, as well as immunogenic cell death, although ER stress or CDDP alone was insufficient to induce CRT exposure and immunogenic cell death. Altogether, our results underscore the contribution of the ER stress response to the immunogenicity of cell death.


Subject(s)
Antineoplastic Agents/pharmacology , Apoptosis/drug effects , Cisplatin/pharmacology , Endoplasmic Reticulum/drug effects , Neoplasms/pathology , S100 Calcium Binding Protein G/metabolism , Calbindin 2 , Cell Line, Tumor , Cell Separation , Endoplasmic Reticulum/metabolism , Endoplasmic Reticulum/pathology , Flow Cytometry , Fluorescent Antibody Technique , Humans , Immunohistochemistry , Microscopy, Confocal , Neoplasms/immunology , Neoplasms/metabolism , Organoplatinum Compounds/pharmacology , Oxaliplatin , Protein Transport/drug effects , S100 Calcium Binding Protein G/immunology , Stress, Physiological/drug effects , Thapsigargin/pharmacology
2.
Rev Stomatol Chir Maxillofac ; 98(1): 7-11, 1997 Jan.
Article in French | MEDLINE | ID: mdl-9273681

ABSTRACT

Primary teratomas rarely occur in the orbit leading to major exophthalmia in the neonate. We report a case in a female infant who presented globular right exophthalmia extending beyond the orbit caused by a teratoma of the orbit. There was right ophthalmoplegia. Computed tomography showed enlargement of the orbit without modification of the bony structure despite the tumor volume. Tumoral enucleation was performed via the transconjunctive route. Histology reported benign mature multi-tissue teratoma. The postoperative course was uneventful. The CT-scan at 6 months evidence the absence of recurrence, outcome which has been confirmed after a 5-years follow-up.


Subject(s)
Orbital Neoplasms/pathology , Teratoma/pathology , Exophthalmos/etiology , Eye Enucleation , Female , Follow-Up Studies , Humans , Infant, Newborn , Ophthalmoplegia/etiology , Orbital Neoplasms/diagnostic imaging , Orbital Neoplasms/surgery , Teratoma/diagnostic imaging , Teratoma/surgery , Tomography, X-Ray Computed , Treatment Outcome
3.
Rev Stomatol Chir Maxillofac ; 90(2): 95-9, 1989.
Article in French | MEDLINE | ID: mdl-2658010

ABSTRACT

A technique of secondary reconstruction of the buccal floor after non interruptive pelvimandibulectomy by thin-split graft enables insertion of a dental prosthesis to assist functional recovery. Results of 46 grafts of this type performed at the Institut Gustave Roussy, Villejuif, France, including 21 cases previously analysed in the Annales d'oto-laryngologie, Paris 1985.


Subject(s)
Mandible/surgery , Mouth Floor/surgery , Mouth Neoplasms/surgery , Skin Transplantation , Adult , Aged , Denture, Complete , Denture, Partial , Female , Humans , Male , Middle Aged , Mouth/physiology , Mouth Neoplasms/pathology , Neoplasm Staging
4.
Rev Stomatol Chir Maxillofac ; 89(6): 342-7, 1988.
Article in French | MEDLINE | ID: mdl-3068788

ABSTRACT

The authors relate the case of two patients of 61 and 64 years old, each one having a high latero-cervical tumor, arching the soft palate, developed from the right vagus nerve and which was a chromaffin paraganglioma. After a recall about the APUD system tumors and the "paraganglioma" concept, they relate their observations at state of the vagal paraganglioma histology, pathology and treatment.


Subject(s)
Head and Neck Neoplasms , Paraganglioma , Vagus Nerve , Female , Head and Neck Neoplasms/diagnostic imaging , Head and Neck Neoplasms/pathology , Head and Neck Neoplasms/surgery , Humans , Middle Aged , Paraganglioma/diagnostic imaging , Paraganglioma/pathology , Paraganglioma/surgery , Tomography, X-Ray Computed
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