Ecological responses and functional significance of paddy crust in the southern Chinese environment.

Paddy Crusts (PC) play a pivotal role in the migration and transformation of heavy metals within paddy ecosystems, situated at the critical intersection of air, water, and soil. This study focused on PC samples from heavy metal-contaminated rice paddies in six southern Chinese provinces. It's the first time we've screened and quantified the impact of nutrition, physicochemical properties, and heavy metals on bacterial diversity in PC. Our results highlight the significant influence of zinc, total nitrogen, and soil manganese on bacterial diversity. Using structural equation models, we identified the pathways through which these three types of environmental factors shape bacterial diversity. Heavy metal indicators and physical and chemical indicators exerted a direct negative effect on bacterial diversity in PC, while nutritional indicators had a direct and significant positive effect on bacterial diversity. Variance partitioning analysis revealed heavy metals had the most significant impact, accounting for 7.77% of the total effect. Moreover, the influence of heavy metals on bacterial diversity increased as diversity decreased, ranging from 3.81% to 42.09%. To remediate specific heavy metal pollution, our proposed method involves cultivating indigenous bacteria by controlling these environmental factors, based on an analysis of the interplay among bacterial diversity, environmental variables, and heavy metal bioconcentration factors. These findings enhance our understanding of PC and provide insights into rice field heavy metal pollution mitigation.

Weakened casual feedback loops following intensive restoration efforts and climate changes in a large shallow freshwater lake.

Understanding how phytoplankton interacts with local and regional drivers as well as their feedbacks is a great challenge, and quantitative analyses of the regulating role of human activities and climate changes on these feedback loops are also limited. By using monthly monitoring dataset (2000-2017) from Lake Taihu and empirical dynamic modelling to construct causal networks, we quantified the strengths of causal feedbacks among phytoplankton, local environments, zooplankton, meteorology as well as global climate oscillation. Prevalent bidirectional causal linkages between phytoplankton biomass (chlorophyll a) and the tested drivers were found, providing holistic and quantitative evidence of the ubiquitous feedback loops. Phytoplankton biomass exhibited the highest feedbacks with total inorganic nitrogen and ammonia and the lowest with nitrate. The feedbacks between phytoplankton biomass and environmental factors from 2000 to 2017 could be classified into two groups: the local environments (e.g., nutrients, pH, transparency, zooplankton biomass)-driven enhancement loops promoting the response of the phytoplankton biomass, and the climate (e.g., wind speed)-driven regulatory loops suppressing it. The two counterbalanced groups modified the emergent macroecological patterns. Our findings revealed that the causal feedback networks loosened significantly after 2007 following nutrient loading reduction and unsuccessful biomanipulation restoration attempts by stocking carp. The strength of enhancement loops underwent marked decreases leading to reduced phytoplankton responses to the tested drivers, while the climate (decreasing wind speed, warming winter)-driven regulatory loops increased- like a tug-of-war. To counteract the self-amplifying feedback loops, the present eutrophication mitigation efforts, especially nutrient reduction, should be continued, and introduction of alternative measures to indirectly regulate the critical components (e.g., pH, Secchi depth, zooplankton biomass) of the loops would be beneficial.

Linking human activities and global climatic oscillation to phytoplankton dynamics in a subtropical lake.

Human activities and climate change are two major stressors affecting lake ecosystems as well as phytoplankton communities worldwide. However, how the temporal dynamics of phytoplankton are directly or indirectly linked to anthropogenic activities and climatic oscillation remains unclear. We assessed the annual trends (1988-2018) in phytoplankton abundance (PA) in Lake Dongting, China and related it to five groups of variables characterizing human activities, global climate oscillation, water nutrients, hydrology, and meteorology. We found a significant increase in PA, urbanization (Upop), total nitrogen (TN), fertilizer application (FA), number of summer days (SU), and the warm speed duration index (WSDI) and a significant decrease in the water discharge of three inlets (TIWD) and the sediment discharge of three inlets (TISD) and four tributaries (FTSD) and the net sediment deposition (NSD). However, no significant annual trends were observed for the number of rainstorm days (R50mm), the simple precipitation intensity index (SDII) and yearly anomalies of El Niño-Southern oscillation events (ENSOi). Cross-correlation Function analyses demonstrated that the operation of the Three George Dam (TGD) strengthened the effects of hydrology, rainfall patterns and ENSOi on phytoplankton, but strongly weakened the association between water nutrients, human activities and phytoplankton abundance. Path analysis revealed that TP, TN, FA, R50 mm as well as WSDI had a direct positive effect on PA, while a direct negative effect was found for ENSOi, NSD and TISD. Human activities (Upop and FA), warming (WSDI and SU), and rainfall patterns (SDII and R50 mm) exerted indirect controls on phytoplankton through changes in water nutrients and hydrology. Climate change (ENSOi) had a direct effect on PA, but also showed twelve indirect pathways via changes in hydrology and meteorology (both positive and negative effects were found). Overall, meteorology contributed most markedly to the variations of PA (29.3%), followed by hydrology (25.3%), human activities (24%), water nutrients (10.5%), and ENSOi (1.9%). Our results highlight a strongly causal connection between human activities as well as global climate change and phytoplankton and the benefits of considering multiple environmental drivers in determining the temporal dynamics of lake biotic communities.

Smartphone-assisted visual ratio-fluorescence detection of hypochlorite based on copper nanoclusters.

A sensitive naked eye and ratio-fluorescence sensor for Curcumin (CCM) and hypochlorite (ClO-) determination based on copper nanoclusters (Cu NCs) was developed. The fluorescence of the Cu NCs can be quenched due to inner filter effect (IFE) between CCM and Cu NCs, and the ratio fluorescence probe was formed. After adding ClO- to Cu NCs-CCM system, the phenolic and methoxy groups of CCM were oxidized to quinones, then the fluorescence of CCM was quenched and the fluorescence of Cu NC was restored. Moreover, the continuous detection of CCM and ClO- is accompanied by the change of solution color. Therefore, CCM and ClO- semiquantitative visual and fluorescence dual channel detection were realized. The detection results show that the detection based on Cu NCs-CCM probe has a wide detection range (0-412 µM) and low detection limit (24 µM), and a good recovery rate is obtained in adulterated milk and tap water detection. Furthermore, smartphone was introduced for image digital colorimetric analysis through the acquisition, recognition and RGB data processing of solution colors, providing an effective scheme for the field rapid detection of hypochlorite.

Different fluorescence emitting copper nanoclusters protected by egg white and double-emission fluorescent probe for fast detection of ethanol.

Green emitting copper nanoclusters (G-Cu NCs), yellow emitting Cu NCs (Y-Cu NCs), orange emitting Cu NCs (O-Cu NCs) and red emitting Cu NCs (R-Cu NCs) were prepared using chicken egg white as the stabilizer by changing the reaction conditions. This is a green, facile and cheap method to explore different color emitting CuNCs by the same precursor and stabilizers. The G-Cu NCs were employed for the detection of ethanol due to their aggregation induced emission enhancement (AIEE) effect. The fluorescence emission of Cu NCs at 526 nm under the excitation of 444 nm can be effectively enhanced in the presence of ethanol due to AIEE effect, thus realizing the quantitative determination of ethanol content in the range 5-60%. In addition, a visual dual-emission fluorescence probe with the combination of G-Cu NCs and silicon nanoparticles (Si NPs/G-Cu NCs) was designed to evaluate ethanol content conveniently and rapidly. Desirable linear relationship is observed between ratio of fluorescence intensity (I525/I441) and ethanol content under the excitation of 383 nm. Visible color transformation of this probe is observed in the ethanol content range 2-20%. Moreover, the ethanol sensing platforms were applied to the detection and evaluation of the alcohol content of liquor, and the recoveries in liquor were in the range 99.7% to 113%, broadening the applications of Cu NCs and providing a sensitive detection method for ethanol.

Facile microwave-assisted synthesis of Ti3C2 MXene quantum dots for ratiometric fluorescence detection of hypochlorite.

A dual-channel "naked-eye" colorimetric and ratio fluorescent probe has been developed based on titanium carbide quantum dots for the detection of curcumin and hypochlorite (ClO-). The fluorescence emission of Ti3C2 MXene quantum dots (Ti3C2 MQDs) is in the range 350-600 nm, and the maximum emission peak is at 430 nm that overlaps with the UV absorption of curcumin at 430 nm to a large extent. This facilitates the fluorescence resonance energy transfer (FRET) between Ti3C2 MQDs and curcumin. When ClO- is added, the phenolic and methoxy groups of curcumin are oxidized to quinones, resulting in the restoration of the fluorescence of Ti3C2 MQD. In addition, the probe designed makes it easier to distinguish colors with the naked eye to detect curcumin and ClO-. The linear detection range of curcumin was 0.05-10 µM, and the detection limit was 20 nM. The linear detection ranges of ClO- are 25-150 µM and 150-275 µM, and the detection limit is 5 µM. This study is the first report on the determination of curcumin and ClO- based on Ti3C2 MQDs by dual-channel "naked-eye" colorimetric and ratio fluorescence method.

A ''naked-eye'' colorimetric and ratiometric fluorescence probe for uric acid based on Ti3C2 MXene quantum dots.

In this work, we developed a ''naked-eye'' colorimetric and ratiometric fluorescence probe for a very important biomarker of uric acid (UA). The method was based on the oxidation of UA by uricase to allantoin and hydrogen peroxide, and then o-Phenylenediamine (OPD) was oxidized to the yellow-colored 2,3-diaminophenazine (oxOPD) in the presence of horseradish peroxidase (HRP) and hydrogen peroxide. The fluorescence emission of glutathione functionalized Ti3C2 MQDs (GSH-Ti3C2 MQDs) centered at 430 nm overlaps with the UV absorption of oxOPD at 425 nm to a large extent, which facilitates fluorescence resonance energy transfer (FRET) between GSH-Ti3C2 MQDs and oxOPD. With the increase of the UA concentration, the emission at 430 nm of GSH-Ti3C2 MQDs is progressively quenched and the emission at 568 nm of oxOPD was gradually increased. Moreover, the probe we designed is easier to distinguish with color change by naked eye for the detection of UA. This is the first report about the determination of UA by a ''naked-eye'' colorimetric and ratiometric fluorescence method combining GSH-Ti3C2 MQDs and uricase/HRP enzymes. This work enables assays to perform fluorescence and visual detection of biomarker in biological fluids based on Ti3C2 MQDs.

A dual-modal fluorometric and colorimetric nanoprobe based on graphitic carbon nitrite quantum dots and Fe (II)-bathophenanthroline complex for detection of nitrite in sausage and water.

A fluorometric and colorimetric dual-mode sensing platform based on graphitic carbon nitrite quantum dots (g-CNQDs) and Fe (II)-bathophenanthroline complex (BPS-Fe2+) was designed to the sensitive detection of nitrite (NO2-) in sausage and water. In this system, the fluorescence of g-CNQDs was quenched by BPS-Fe2+ complex due to the inner filter effect (IFE). When NO2- was present, Fe2+ was oxidized by nitrite to form BPS-Fe3+ complex with BPS, leading to the recovery of the fluorescence from g-CNQDs. Therefore, we constructed a "turn-off-on" fluorescence probe for detection of NO2-. Moreover, with the increase of NO2- concentration, the color of the solution changed from red to colorless, so the UV-vis measurements and on-site visual detection were realized. The method is capable of detecting NO2- in the concentration range of 2.32-34.8 µM with good selectivity and high sensitivity. In addition, the method has the potential to determine NO2- in water samples and sausage samples.

ε-Poly-L-lysine-protected Ti3C2 MXene quantum dots with high quantum yield for fluorometric determination of cytochrome c and trypsin.

Titanium carbide quantum dots functionalized with ε-poly-L-lysine (PLL) were synthesized by sonication cutting and hydrothermal synthesis. The deprotonated Ti3C2 MXene quantum dots (Ti3C2 MQDs) exhibit excitation wavelength-dependent blue photoluminescence with typical excitation/emission peaks at 330/415 nm and a quantum yield of 22% due to strong quantum confinement. The fluorescence of ε-poly-L-lysine protected Ti3C2 MQDs (PLL-protected Ti3C2 MQDs) is reduced via an inner filter effect after the addition of cytochrome c (cyt-c). Response to cyt-c is linear in the 0.2 to 40 µM concentration range and the detection limit is 20.5 nM. In the presence of trypsin, cyt-c is hydrolyzed to small peptides, and the Fe3+ ion in cyt-c probably is reduced to Fe2+ with the aid of the digestive enzyme. This results in the restoration of the blue fluorescence of the modified MQDs. Fluorescence increases linearly in the 0.5 to 80 µg mL-1 trypsin concentration range with the detection limit of 0.1 µg mL-1. The method was successfully applied to the determination of cyt-c and trypsin in spiked serum samples. Graphical abstractSchematic of a method for the fluorometric "turn-off-on" determination of cytochrome c and trypsin based on ε-poly-L-lysine (PLL) protect MXene quantum dots (Ti3C2 MQDs).

Carbon dots co-doped with nitrogen and chlorine for "off-on" fluorometric determination of the activity of acetylcholinesterase and for quantification of organophosphate pesticides.

Nitrogen and chlorine dually-doped carbon dots (N,Cl-CDs) were hydrothermally prepared starting from 4-chloro-1,2-diaminobenzene and dopamine. The N,Cl-CDs exhibit strong orange fluorescence, with excitation/emission maxima at 420/570 nm and a relative high quantum yield (15%). The N,Cl-CDs were employed to detect acetylcholinesterase (AChE) activity and organophosphate pesticides (OPs) which are enzyme inhibitors. Acetylthiocholine is enzymatically split by AChE to produce thiocholine which triggers the decomposition of Ellmans's reagent to form a yellow colored product (2-nitro-5-thiobenzoate anion). The product causes an inner filter effect (IEF) on the fluorescence of the N,Cl-CDs. Fluorescence decreases linearly in the 0.017 to 5.0 Unit·L-1 AChE activity range, and the detection limit is 2 mUnit·L-1. If organophosphates are present, the activity of AChE becomes increasingly blocked, and this leads to a less expressed IFE and an increasing recovery of fluorescence. This was used for the quantification of OPs. Response is linear in the 0.3-1000 µg·L-1 OP concentration range with a 30 ng·L-1 detection limit. Graphical abstractSchematic representation of the synthesis of nitrogen and chlorine dually-doped carbon dots (N,Cl-CDs) and the recognition of organophosphate pesticides by N,Cl-CDs.

Fabrication of Fluorescence Turn-off-on Sensor Based on g-C3N4 Quantum Dots and MgFe Layered Double Hydroxide for the Detection of Citrate.

We fabricated a new and selective fluorescent sensor for the detection of citrate by employing g-C3N4 quantum dots (g-CNQDs) and MgFe layered double hydroxide (MgFe-LDH). The g-CNQDs interacted with MgFe-LDH via electrostatic interaction and the fluorescence of g-CNQDs was effectively quenched by MgFe-LDH due to inner filter effect. Upon addition of citrate, the fluorescence of the g-CNQDs was significantly enhanced, arising from the replacement of g-CNQDs by citrate because citrate competed with g-CNQDs to form more stable complexes with MgFe-LDH. Therefore, we developed a "turn-off-on" fluorescence assay method for the detection of citrate. This method enabled the selective detection of citrate with linear range of 0.5-3.0 µM and 3.0-10.0 µM with a 12.3 nM limit of detection. This method exhibited advantages including easy preparation, environmentally friendly process and rapid response toward citrate.

Determination of the activity of alkaline phosphatase based on aggregation-induced quenching of the fluorescence of copper nanoclusters.

A rapid method is described for synthesis of copper nanoclusters (CuNCs) by utilizing L-histidine as the stabilizer and ascorbic acid (AA) as the reductant. The CuNCs display blue-green fluorescence with excitation/emission peaks at 390/485 nm. A sensitive fluorometric assay was worked out for determination of alkaline phosphatase (ALP) activity. If the ALP substrate p-nitrophenylphosphate (PNPP) is enzymatically hydrolyzed, it forms p-nitrophenol (PNP) which reduces the fluorescence of CuNCs because its absorption band at 410 nm overlaps the excitation peak of CuNCs at 390 nm. In addition, the amino groups and imidazole groups on the surface of CuNCs possibly form a complex with the phenol groups of PNP. This induces aggregation-induced quenching of the fluorescence of CuNCs. The fluorescent probe has a linear analytical range that extends from 0.5 mU·mL-1 to 40 mU·mL-1 and a detection limit of 45 µU·mL-1. Graphical abstract Schematic illustration of a fluorometric assay for alkaline phosphatase (ALP) activity that uses L-histidine protected copper nanoclusters (CuNCs), aggregation-induced quenching, and the inner filter effect between PNP and CuNCs.

Poly(styrene-4-sulfonate)-protected copper nanoclusters as a fluorometric probe for sequential detection of cytochrome c and trypsin.

Stable copper nanoclusters (CuNCs) were prepared by utilizing D-penicillamine as both the stabilizer and reductant. The emission of the CuNCs (with excitation/emission peaks at 390/645 nm) is largely stabilized by coating with poly(sodium-p-styrenesulfonate) (PSS). Cytochrome c (Cyt c) quenches the fluorescence of the PSS-coated CuNCs, and this effect was exploited to design a quenchometric fluorometric assay for Cyt c. If trypsin is added to the loaded CuNCs, it will hydrolyze Cyt c to form peptide fragments, and fluorescence is gradually restored. A highly sensitive and fluorometric turn-off-on assay was constructed for sequential detection of Cyt c and trypsin. The linear ranges for Cyt c and trypsin are from 8.0 nM to 680 nM, and from 0.1 to 6.0 µg mL-1, and the lower detection limits are 0.83 nM and 20 ng mL-1 for Cyt c and trypsin, respectively. Graphical abstract Schematic illustration of the fluorometric assay for trypsin based on the electron transfer between poly(p-styrenesulfonate)-protected copper nanoclusters (PSS-CuNCs) and cytochrome c (Cyt c).

Ultrasensitive detection of heparin by exploiting the silver nanoparticle-enhanced fluorescence of graphitic carbon nitride (g-C3N4) quantum dots.

A composite (Ag-g-CNQDs) was prepared from graphitic carbon nitride quantum dots and silver nanoparticles by water phase synthesis. Aided by metal-enhanced fluorescence, the composite exhibits excitation-dependent red emission with a peak at 600 nm with a quantum yield of 21%. If the composite is coated with polyethylenimine (PEI) to form the Ag-g-CNQD/PEI complexe, fluorescence is strongly reduced. Upon addition of heparin, the fluorescence of the system is enhanced because PEI has a higher affinity for heparin than Ag-g-CNQDs. The effect was used to design a fluorometric  assay for heparin. The emission at 600 nm increases linearly in the 0.025 to 2.5 µM heparin concentration range, with a 8.2 nM limit of detection. Graphical abstract Schematic illustration for fabricating a composite consisting of silver nanoparticles and graphitic carbon nitride quantum dots (Ag-g-CNQDs). Its red fluorescence is weak in presence of polyethyleneimine but restored on addition of heparin. This forms the basis for a new method for heparin detection.

Dually emitting gold-silver nanoclusters as viable ratiometric fluorescent probes for cysteine and arginine.

Glutathione coated gold and silver nanoclusters (GSH-Au/AgNCs) were synthesized by one-pot reduction methods and are found to be viable fluorescent nanoprobes for cysteine (Cys) and arginine (Arg), with good selectivity over other amino acids. The GSH-Au/AgNCs have two emissions at 616 nm and 412 nm when excited at 360 nm. With the increased concentration of Cys, the ratio of the emission intensities (I616/I412) linearly decreases with Cys in concentration ranging from 0.05 to 10 µM and from 10 to 50 µM, respectively. With increased concentrations of Arg, the ratio of I616/I412 linearly decreases with Arg concentration ranging from 0 to 50 µM and from 50 to 100 µM, respectively. The probe was applied to the determination of Cys and Arg in spiked samples of serum and urine where it gave good recoveries. Graphical abstract Glutathione-coated gold and silver nanoclusters (GSH-Au/AgNCs) were synthesized by one-pot reduction and are found to be viable fluorescent nanoprobes for cysteine (Cys) and arginine (Arg).

Fluorescence Detection of p-Nitrophenol in Water Using Bovine Serum Albumin Capped ag Nanoclusters.

We developed a sensitive and selective method for detection of 4-Nitrophenol (PNP) using Ag nanoclusters (NCs) as fluorescence probe. Bovine serum albumin (BSA) was used as the template for preparing Ag NCs via wet chemistry. The fluorescence of as-prepared Ag NCs was significantly quenched in the presence of PNP, showing excellent linear relationships between the quenching degree of the fluorescence intensity and the concentrations of PNP in the range of 0.5 µM to 60 µM. The fluorescence quenching mechanism was also investigated. Furthermore, this method was applied for the recognition of PNP in environmental samples.

Inner filter effect based fluorometric determination of the activity of alkaline phosphatase by using carbon dots codoped with boron and nitrogen.

Boron and nitrogen codoped carbon dots functionalized with cyclodextrin (ß-CD-N/B-C-dots) were obtained from ß-cyclodextrin. The material displays strong fluorescence (with excitation/emission peak wavelengths of 400/500 nm) and was characterized by UV-vis, transmission electron microscopy and FTIR. If the substrate p-nitrophenylphosphate is enzymatically cleaved by alkaline phosphatase (ALP), a yellow product is formed whose absorption overlaps the excitation spectrum of the ß-CD-N/B-C-dots. Hence, fluorescence is reduced due to an inner filter effect. In additon, the ß-CD cavity offers a pocket for substrate recognition. The findings were used to design a method for the determination of the activity of ALP. It has a working range that extends from 0.003 to 5.5 U·L-1, with a 0.3 mU·L-1 detection limit. The method is fast, simple, inexpensive, and highly sensitive and selective. Graphical abstract Schematic of an inner filter effect based probe for alkaline phosphatase based on the use boron and nitrogen co-doped carbon dots (N/B-C-dots) modified with ß-cyclodextrin (ß-CD). PNPP: p-Nitrophenylphosphate; PNP: p-Nitrophenol anion.

Brightly near-infrared to blue emission tunable silver-carbon dot nanohybrid for sensing of ascorbic acid and construction of logic gate.

Recently, carbon dots (CDs) have drawn extensive attention owing to their unique properties. While more and more research had been focusing on the exploration of CDs as fluorescence sensors, the blue or green fluorescence emission of CDs restricts further applications of CDs, particularly in the biology-relevant fields due to the commonly blue auto-fluorescence of biological matrix. Therefore, the preparation of CDs with strong emission at long wavelengths is highly desirable. For these situations, a facile, straightforward and extra-reductant needless method was established to fabricate silver-carbon dot nanohybrid with enhanced near-infrared fluorescence through metal-enhanced fluorescence (MEF). We applied the Ag-CD nanohybrid as a probe to detect antioxidants such as ascorbic acid in near-infrared window, and the probe possesses a linear range of 0.2-60µM. More importantly, this sensor could not only function in aqueous solution, but also display well selective response in intricate biological fluids. In addition, an IMPLICATION logic gate was constructed based on the unique characteristics of Ag-CD nanohybrid which presages more opportunities for application in single and multiple biological sensing.

Fluorescent Detection of Phosphate in Aqueous Solution Based on Near Infrared Emission Ag2S QDs/Metal - Organic Shell Composite.

A novel fluorescence method for sensitive and selective detection of phosphate was developed based on near infrared emission Ag2S QDs/ Metal - Organic Shell Composite via the deposition of metal-organic (zinc-nitrogen) coordination shell around Ag2S QDs . Under optimal conditions described, the fluorescence intensity of the composite was decreased at 685 nm in the presence of phosphate, which was linearly related to the concentration of phosphate in the range of 0. 7 to 4.2 µM and 11.2 to 88.2 µM with the relative correlation coefficient of R2 = 0.998 and 0.987 respectively and detection limit as low as 6 nM. In addition, the proposed method was successfully utilized in serum samples, tap water and Yangtze River water samples with the recoveries ranged from 94.76 to 100.86 %, which presaged more opportunities for application in related bioassay and water sample researches.

"Turn-Off-On" Fluorescence Switching of Ascorbic Acid-Reductive Silver Nanoclusters: a Sensor for Ascorbic Acid and Arginine in Biological Fluids.

In this report, a novel one-step chemical reduction method was reported for synthesis of water-soluble and stable fluorescent glutathione-templated silver nanocluster (GSH-Ag NCs) with ascorbic acid as an environmental-friendly reducing agent. On the basis of an oxidoreduction-induced fluorescence quenching mechanism, the prepared GSH-Ag NCs found to act as a cheap, non-toxic and highly sensitive "turn-off" fluorophore for ascorbic acid (AA). Furthermore, the fluorescence of the fluorophore/AA system could be recovered through addition of arginine (Arg), which made the system function as a highly selective "turn-on" sensor for arginine. Therefore, a "turn-off-on" switch sensor was proposed for detection of AA and Arg. Under optimized conditions, the probe gives a fluorescent response that is linear in the 2-300 µM concentration range of AA, with a detection limit of 0.1 µM. The probe for Arg, in turn, has a linear range in the 10-180 µM concentration range, and the limit of detection is 0.5 µM. In addition, the developed method showed great accuracy when employed to detect AA and Arg in human urine and serum, which shows its great potential in biological molecular recognition applications.