The molecular mechanism of serum microRNA124b induced coronary heart disease by inducing myocardial cell senescence.

The article "The molecular mechanism of serum microRNA124b induced coronary heart disease by inducing myocardial cell senescence" by Department of Cardiology, Qilu Hospital of Shandong University, Jinan, Shandong, China, published in Eur Rev Med Pharmacol Sci 2018; 22 (7): 2070-2076 has been withdrawn.

The molecular mechanism of serum microRNA124b induced coronary heart disease by inducing myocardial cell senescence.

OBJECTIVE: The incidence and mortality of coronary heart disease are rapidly increasing in recent years. Myocardial cell dysfunction and cell senescence may play a role in coronary heart disease. MicroRNA controls a variety of biological processes, but leaving its role in coronary heart disease has yet to be explored. PATIENTS AND METHODS: Patients with coronary heart disease were regarded as subjects, and healthy volunteers as the control, on both of which microRNA124b level of serum was studied by Real-time PCR, and the heart function of patients was detected by using ultrasound. The relationship between serum microRNA124b level and cardiac function was analyzed along with the model of rat coronary artery disease; the level of aging proteins P21 and P53 in cardiac muscle cells was also tested. RESULTS: MicroRNA124b in the serum of patients with coronary heart disease was increased, and the heart function of patients was decreased (p < 0.05). Serum level of microRNA124b in a rat model of coronary heart disease was increased, and the cardiac function was decreased (p < 0.05). When myocardial cell appeared ageing, the level of P21 and P53 was increased, and the level of microRNA124b was related with P53. CONCLUSIONS: The level of microRNA124b in the serum of coronary heart disease patients and rat model may be related to the occurrence of coronary heart disease; microRNA124b may lead to the occurrence of coronary heart disease by causing cell senescence.

Features of stroke in Chinese diabetes patients: a hospital-based study.

In this study, demographic characteristics, risk factors, stroke subtypes and outcome were compared in 2532 patients with and without diabetes hospitalized for first-ever stroke. Diabetes was present in 471 (18.6%) of the patients. Patients with diabetes presented more frequently with ischaemic stroke (92.1% versus 71.3%), especially lacunar infarction (41.2% versus 35.2%), compared with non-diabetics. Cerebral haemorrhage was less frequent in diabetics than non-diabetics (4.2% versus 18.1%). In-hospital mortality rates from ischaemic stroke were similar in the two groups (18.2% in diabetics and 16.9% in non-diabetics). Predictors of in-hospital mortality in diabetic patients included decreased consciousness, congestive heart failure and atrial fibrillation. In conclusion, stroke in diabetic patients was different to stroke in non-diabetic patients: in diabetics the frequency of cerebral haemorrhage was lower and the rate of lacunar infarct syndrome was higher, but in-hospital mortality from ischaemic stroke was not increased. Clinical factors evident at the onset of stroke have a major influence on in-hospital mortality and may help clinicians provide a more accurate prognosis.

Synthesis and biological activities of diquaternary dipiperazinium salts containing dithiocarboxyl groups.

A series of diquaternary dipiperazinium salts containing dithiocarboxyl groups 6a-f and 9 were synthesized and evaluated for their analgesic and sedative activities. The result showed that the presence of two quaternary ammonium cations and the distance between them are very important for the activities of the salts. Compound 6b exhibited the best activities (at dose 2 mg/kg, analgesic, 57%; sedative, 59%) among compounds 6a-f. Compound 9 not only showed the most potent analgesic (85.4%, dose 1 mg/kg) and sedative (93.1%, dose 1 mg/kg) activities, but also exhibited anticancer activity against KB (68.7%, dose 10 microM).

[Synthesis and anti-tumor activities of 1,4-bis[3-(amino-dithiocarboxy)propionyl] piperazine derivatives].

AIM: To synthesize piperazine derivatives and screen anti-tumor compounds with higher activity and lower toxicity. METHODS: Selecting 1,4-bis(3-bromopropionyl)piperazine as leading compound, a series of 1,4-bis[3-(amino-dithiocarboxy)propionyl] piperazine derivatives (4a-j) were synthesized through the use of aminodithiocarboxylate. All the synthetic compounds (4a-j) were tested for their anti-tumor activity against eight kinds of tumor cells. RESULTS: Compounds (4a-j) are new compounds, among them, compounds 4c, 4d and 4e showed anti-tumor activity against HL-60. The inhibition of compounds 4c, 4d and 4e against HL-60 are 44%, 90% and 70% respectively, at the concentration of 10 mumol.L-1. However, the inhibition of the other kinds of anti-tumor cells are not distinctive. CONCLUSION: These results suggest that this may be one of the effective routes to improve the anti-tumor activity and reduce the toxicity of 1,4-bis(3-bromopropionyl)piperazine.

Quantification of left-side intracardiac pressures and gradients using mitral and aortic regurgitant velocities by simultaneous left and right catheterization and continuous-wave Doppler echocardiography.

Noninvasive determination of left-side intracardiac pressures is of clinical importance in many cardiac diseases. To test the reliability and accuracy of left-side intracardiac pressure measurements by continuous-wave Doppler echocardiography, using left-side valvular regurgitations, 47 patients with mitral regurgitation, with or without associated aortic regurgitation, underwent simultaneous Doppler and left and right catheterization. Doppler-derived left atrial and ventricular end-diastolic pressures were respectively estimated by subtracting mitral regurgitant gradient from systolic blood pressure and by diastolic blood pressure minus aortic regurgitant gradient. There were high correlations of mitral (r = 0.961) and aortic regurgitant gradients (r = 0.896) and of left atrial (r = 0.945) and ventricular end-diastolic pressures (r = 0.854) between noninvasive and invasive measurements. Also, agreement analyses showed that there was close agreement between the two technical measurements for each parameter. The present study concluded that continuous-wave Doppler echocardiography provides a reliable and accurate method for the noninvasive evaluation of left-side intracardiac pressures and gradients in patients with mitral and aortic regurgitations.

Reliability and accuracy of measurement of transductal gradient by Doppler ultrasound.

Simultaneous continuous wave Doppler echocardiography, aortic and pulmonary artery pressure measurements were performed during cardiac catheterization in 46 patients with patent ductus arteriosus. Doppler-derived systolic, mean and diastolic transductal gradients correlated well with those measured by catheterization, respectively (r = 0.972, SEE = 6.8 mmHg; r = 0.965, SEE = 5.4 mmHg; r = 0.939, SEE = 6.2 mmHg), and there were clinically acceptable agreements between the two technical measurements. It is concluded that Doppler echocardiography is a reliable and accurate technique for noninvasive estimation of transductal gradients.

[Noninvasive estimation of the pulmonary artery pressures in patent ductus arteriosus with continuous-wave Doppler echocardiography].

To develop a new method for noninvasive measuring of the pulmonary artery pressure (PAP) in patient ductus arteriosus (PDA), left and right heart catheterization (Cath) and continuous-wave Doppler echocardiography (CWD) were performed simultaneously in 30 cases with PDA. The results showed that there was high correlation of the systolic peak pressure gradient (delta pp), the end-diastolic pressure gradient (delta Pd) and the mean pressure gradient (delta Pm), across PDA measured with the two techniques (r = 0.99, 0.96 and 0.98, respectively). The CWD-derived pulmonary artery systolic (PASP), diastolic (PADP) and mean (PAMP) pressure, which were estimated by subtracting delta Pp, delta Pd and delta Pm from the brachial artery systolic, diastolic and mean pressure, correlated well with the corresponding PAP measured with Cath (r = 0.92, 0.89 and 0.94, respectively). It is concluded that CWD offers a reliable technique for estimating shunt pressure gradients and PAP in patients with PDA.

[Diagnosis of pulmonary hypertension by pulsed Doppler echocardiography--comparison with cardiac catheterization].

31 patients undergoing diagnostic cardiac catheterization for suspected congenital heart disease and 30 normal subjects were examined by pulsed Doppler echocardiography. Spectrum analysis of the shape of flow velocity curve and instantaneous flow velocities of the pulmonary artery during systolic period was made and their ratio calculated. The results showed that there was a remarkable difference between the flow velocity curve of pulmonary hypertension (PH) and that of normal pulmonary pressure. Combining with the characteristics of pulmonary arterial hemodynamics, a preliminary exploration was made on the mechanism of flow velocity curve changes. A ratio of flow velocities--PV% was proposed. The sensitivity and specificity for diagnosing PH with PV% were 94% and 100% respectively. There was a high correlation between PV% and pulmonary artery systolic pressure (r = -0.84) or pulmonary artery mean pressure (r = -0.82). It is concluded that PV% may eliminate many factors that influence the pulmonary artery hemodynamics and is a reliable, practical qualitative and quantitative index for diagnosing PH noninvasively.