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1.
Article in English | MEDLINE | ID: mdl-26478265

ABSTRACT

The Eurasian perch (Perca fluviatilis) is a freshwater carnivorous species of high interest to diversify inland aquaculture. However, little is known about its ability to bioconvert polyunsaturated fatty acids (PUFAs) from plant oils into long chain polyunsaturated fatty acids (LC-PUFAs). In this study, special attention has been given to the fatty acid desaturase 2 (FADS2) which is commonly described to be a rate-limiting enzyme of the LC-PUFA biosynthesis. This work reports on the cloning, tissue expression and functional characterization of the Eurasian perch fads2, but also on the cloning of two alternative splicing transcripts named fads2-AS1 and fads2-AS2. The fads2 cDNA cloned is composed of an open reading frame (ORF) of 1338 nucleotides (nt) and encodes a protein of 445 amino acids. This deduced amino acid sequence displays the typical structure of microsomal FADS2 including two transmembrane domains and an N-terminal cytochrome b5 domain with the "HPGG" motif. Quantitative real-time PCR assay of fads2, fads2-AS1 and fads2-AS2 expressions revealed that the fads2 transcript was mainly expressed in the liver and intestine and exhibited a typical gene expression pattern of freshwater species while fads2-AS1 and fads2-AS2 genes were highly expressed in the brain, followed by the liver and intestine. Functional characterization of Eurasian perch FADS2 in transgenic yeast showed a fully functional Δ6 desaturation activity toward C18 PUFA substrates, without residual Δ5 and Δ8 desaturase activities.


Subject(s)
Linoleoyl-CoA Desaturase/genetics , Linoleoyl-CoA Desaturase/metabolism , Perches/genetics , Amino Acid Sequence , Animals , Base Sequence , Cloning, Molecular , Humans , Linoleoyl-CoA Desaturase/chemistry , Molecular Sequence Data , Phylogeny , Protein Transport , RNA, Messenger/genetics , RNA, Messenger/metabolism
3.
Fish Shellfish Immunol ; 47(2): 782-96, 2015 Dec.
Article in English | MEDLINE | ID: mdl-26497094

ABSTRACT

This study was designated to investigate the effects of dietary fish oil (FO diet) replacement by linseed oil (LO diet) on regulation of immune response and disease resistance in Eurasian perch (Perca fluviatilis). A control diet containing fish oil (FO = cod liver oil) and characterized by high levels of n-3 high LC-PUFA (6% EPA, 7.5% of total fatty acids (FAs)) was compared to linseed oil diet (LO diet) composed of low LC-PUFA contents (1% EPA, 2.3% DHA of total FAs) but high C18 fatty acids levels. The experiment was conducted in quadruplicate groups of 80 fish each. After 10 weeks of feeding, the innate immune status was evaluated in various organs (liver, spleen, and head-kidney) (feeding condition). Two days later, a bacterial challenge was performed on fish from 2 rearing conditions: fish infected with Aeromonas salmonicida (bacteria condition) and fish injected with sterile medium but maintained in the same flow system that fish challenged with bacteria (sentinel condition). Three days after injection of bacteria, a significant decrease of lymphocyte, thrombocyte and basophil populations was observed while neutrophils were not affected. In addition, plasma lysozyme activity and reactive oxygen species production in kidney significantly increased in fish challenged with A. salmonicida while the plasma alternative complement pathway activity was not affected. Increase of plasma lysozyme activity as well as reactive oxygen species production in spleen and kidney of sentinel fish suggest that these immune defenses can also be activated, but at lower bacteria concentration than infected fish. No differences in leucocyte populations, plasma lysozyme and alternative complement pathway activities were observed between dietary treatments. Similarly, expression of genes related to eicosanoid synthesis in liver were not affected by the dietary oil source but were strongly stimulated in fish challenged with A. salmonicida. These findings demonstrated that the use of linseed oil does not deplete the innate immune system of Eurasian perch juveniles.


Subject(s)
Dietary Fats, Unsaturated/pharmacology , Fish Diseases/immunology , Gram-Negative Bacterial Infections/veterinary , Immunity, Innate , Linseed Oil/pharmacology , Perches , Aeromonas salmonicida/physiology , Animal Feed/analysis , Animals , Diet/veterinary , Fatty Acids, Unsaturated/metabolism , Fish Diseases/microbiology , Gene Expression , Gram-Negative Bacterial Infections/immunology , Gram-Negative Bacterial Infections/microbiology , Time Factors
4.
Lipids ; 50(12): 1219-32, 2015 Dec.
Article in English | MEDLINE | ID: mdl-26439838

ABSTRACT

The aim of this study was to evaluate the impact of replacing dietary fish oil (FO) with linseed oil (LO) on growth, fatty acid composition and regulation of lipid metabolism in Eurasian perch (Perca fluviatilis) juveniles. Fish (17.5 g initial body weight) were fed isoproteic and isoenergetic diets containing 116 g/kg of lipid for 10 weeks. Fish fed the LO diet displayed lower growth rates and lower levels of DHA in the liver and muscle than fish fed the FO diet, while mortality was not affected by dietary treatment. However, DHA content recorded in the liver and muscle of fish fed the LO diet remained relatively high, despite a weight gain of 134 % and a reduced dietary level of long-chain polyunsaturated fatty acids (LC-PUFA), suggesting endogenous LC-PUFA biosynthesis. This was supported by the higher amounts of pathway intermediates, including 18:4n-3, 20:3n-3, 20:4n-3, 18:3n-6 and 20:3n-6, recorded in the liver of fish fed the LO diet in comparison with those fed the FO diet. However, fads2 and elovl5 gene expression and FADS2 enzyme activity were comparable between the two groups. Similarly, the expression of genes involved in eicosanoid synthesis was not modulated by dietary LO. Thus, the present study demonstrated that in fish fed LO for 10 weeks, growth was reduced but DHA levels in tissues were largely maintained compared to fish fed FO, suggesting a physiologically relevant rate of endogenous LC-PUFA biosynthesis capacity.


Subject(s)
Diet/veterinary , Dietary Fats, Unsaturated/administration & dosage , Linseed Oil/administration & dosage , Lipid Metabolism , Muscle, Skeletal/metabolism , Perches/metabolism , Seafood/analysis , Acetyltransferases/metabolism , Animals , Aquaculture , Dietary Fats, Unsaturated/metabolism , Fatty Acid Desaturases/metabolism , Fatty Acid Elongases , Fatty Acids, Omega-3/administration & dosage , Fatty Acids, Omega-3/analysis , Fatty Acids, Omega-3/metabolism , Fatty Acids, Omega-6/administration & dosage , Fatty Acids, Omega-6/analysis , Fatty Acids, Omega-6/metabolism , Fish Oils/metabolism , Fish Proteins/metabolism , France , Gene Expression Regulation, Developmental , Linseed Oil/metabolism , Liver/enzymology , Liver/growth & development , Liver/metabolism , Muscle, Skeletal/growth & development , Perches/growth & development , Survival Analysis , Weight Gain
5.
Encephale ; 40(3): 276-85, 2014 Jun.
Article in French | MEDLINE | ID: mdl-24954172

ABSTRACT

With the publication of the DSM-5, the alcoholic disease becomes Alcohol-use disorders taking into account dependence and damages according to a gradient of severity. This conceptual evolution should improve the screening of people affected by this chronic and progressive disease associated with a heavy impact on morbidity and mortality. This identification is provided by the clinical interview and examination. Its sensitivity can be improved by questionnaires and biological markers. Damage and related pathologies have to be systematically revealed. In this context, the MoCA test allows the early detection of cognitive disorders. Care management aims for a change in consumption and consists in supporting the patient in his/her approach. Care management should be personalized, appropriated to the patient's informed choice and graded according to disorders' severity. This includes a psychosocial intervention which can be associated with drugs. Abstinence is no longer the only alternative: decreasing consumption with the aim of regulation has been shown interesting in the reduction of harmful consumption. When therapeutic withdrawal turns out to be necessary, use of a treatment should not be systematic. If necessary, benzodiazepines remain the first-line treatment with a limited duration of prescription. According to his/her profile, the patient can receive a long-term treatment to help to maintain abstinence, prevent relapse or reduce consumption. There is evidence that identifying and involving patients in their care significantly improve their health. Effective treatments are available for treating alcohol-use disorders. However, only a relatively small number of patients receive specific management appropriated to their needs and according to concrete goals.


Subject(s)
Alcohol-Related Disorders/diagnosis , Alcohol-Related Disorders/rehabilitation , Alcoholism/diagnosis , Alcoholism/rehabilitation , Alcohol Drinking/psychology , Alcohol-Related Disorders/psychology , Alcoholism/psychology , Case Management , Cognition Disorders/diagnosis , Cognition Disorders/psychology , Cognition Disorders/rehabilitation , Diagnostic and Statistical Manual of Mental Disorders , Early Diagnosis , Humans , Interview, Psychological , Mass Screening , Neuropsychological Tests , Patient Care Planning , Recurrence , Surveys and Questionnaires , Temperance
6.
Comp Biochem Physiol B Biochem Mol Biol ; 156(4): 237-43, 2010 Aug.
Article in English | MEDLINE | ID: mdl-20363355

ABSTRACT

Supplies of marine fish oils are limited, and continued growth in aquaculture production dictates that lipid substitutes in fish diets must be used without compromising fish health and product quality. In this study, the total substitution of a fish meal and fish oil by a blend of vegetable meals (corn, soybean, wheat and lupin) and linseed oil in the diet of European sea bass (Dicentrachus labrax) was investigated. Two groups of European sea bass were fed with fish diet (FD) or vegetable diet (VD) for 9months. VD, totally deprived of eicosapentaenoate (EPA; 20:5n-3) and docosahexaenoate (DHA; 22:6n-3), revealed a nutritional deficiency and affected growth performance. Whilst VD induced a significant increase in fatty acid desaturase 2 (FADS2) and sterol binding regulatory element-binding protein 1 (SREBP-1) mRNA levels, the desaturation rate of [1-(14)C]18:3n-3 into [1-(14)C]18:4n-3, analysed in microsomal preparations using HPLC method, did not show an upregulation of FADS2 activities in liver and intestine of fish fed VD. Moreover Western-blot analysis did not revealed any significant difference of FADS2 protein amount between the two dietary groups. These data demonstrate that sea bass exhibits a desaturase (FADS2) activity whatever their diet, but a post-transcriptional regulation of fads2 RNA prevents an increase of enzyme in fish fed a HUFA-free diet. This led to a lower fish growth and poor muscle HUFA content.


Subject(s)
Bass/metabolism , Diet , Fatty Acid Desaturases/metabolism , Fish Proteins/metabolism , Animal Feed , Animals , Aquaculture , Bass/genetics , Bass/growth & development , Fatty Acid Desaturases/genetics , Fatty Acids/analysis , Fish Proteins/genetics , Gene Expression Regulation , Intestines/enzymology , Liver/enzymology , PPAR alpha/genetics , PPAR alpha/metabolism , Sterol Regulatory Element Binding Protein 1/genetics , Sterol Regulatory Element Binding Protein 1/metabolism , Vegetables
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